FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 mx12-3.3410000000cdba.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 mx12-3.3410000000cdba.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17839626
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT254390.14259828092808674TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA251690.1410847962844064TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT21800.029.8614461
CACACGT121750.023.0092712-13
TATGCCG105850.023.00578348-49
CGTATGC107600.022.98176446-47
CTCGTAT110050.022.64312744-45
ACATGCG42650.020.8159734
CATGCGT42600.020.728965
CGTCTGA141600.019.71665816-17
GCGTATT31250.018.8408012
AGCACAC153750.018.38836910-11
AGTCACA152600.018.32238628-29
ACATGTC156450.018.0246332-33
CACGTCT162300.017.24584614-15
TAAGTCG31350.017.1125149
TCCGTAC43750.016.9285855
ATGTCAG168200.016.73823734-35
TCTGAAC171450.016.42142518-19
TCACATG173200.016.21219830-31
CCAGTCA181900.015.55276126-27
CTGACAT104950.015.0767781
Produced by FastQC (version 0.11.2)