FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 mx0-3.3410000000cbd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 mx0-3.3410000000cbd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20943685
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT976540.4662694267985792TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA381920.1823556838254586TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACGT185950.036.77083634-35
TACGTAA185350.036.72626536-37
TATGCCG191600.035.37390548-49
ACCGTAC194700.035.3605132-33
CGTATGC193650.035.2525546-47
CGTAATC191250.034.54957638-39
CTCGTAT203500.032.79923244-45
GAGCACA319200.031.6817159
CGTCTGA221700.031.29104216-17
CACACGT226700.030.82082612-13
AGAGCAC326950.030.7718
CACGTCT226000.030.76921714-15
GATCGGA338950.029.13471
ATCGGAA340600.028.9707162
CGGAAGA352300.028.6515254
AGTCACC242750.028.42723328-29
TAATCTC233700.028.2429140-41
CCGTACG176900.027.90639932-33
TCGGAAG355900.027.761993
GTACGTA181200.027.2193734-35
Produced by FastQC (version 0.11.2)