FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 m12-3.3410000000ccd5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 m12-3.3410000000ccd5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18952035
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTA480090.2533184431117819TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT349230.1842704490573176TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG165850.026.41457648-49
CACACGT169600.026.34442512-13
CGTATGC169350.026.00547646-47
CTCGTAT170700.024.81126644-45
CGTCTGA181900.024.7328316-17
AGATCGG223500.022.859661
CACGTCT202050.022.17227714-15
ACAGTCA205000.021.8901532-33
ATCGGAA236800.021.5747263
AGTCACA209900.021.32088728-29
AGCACAC211850.021.23463410-11
AGAGCAC247200.021.146069
CCAGTCA220550.020.06343826-27
TCACAGT223250.020.003930-31
AGTCAAC226200.019.77749834-35
TCGGAAG263700.019.6433494
TCTGAAC239100.019.03357318-19
GATCGGA275600.018.6952172
CGGAAGA289600.017.984945
AACAATC263850.016.79480438-39
Produced by FastQC (version 0.11.2)