FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 kx24-2.3410000000cdfe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 kx24-2.3410000000cdfe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26285590
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT554190.21083414905276998TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA308200.020.8732959
AGAGCAC301200.020.8067048
AGTCACG157200.020.3397728-29
ATCGGAA306950.019.8010312
CGTATGC171350.018.7980446-47
CACGTGA167500.018.6500430-31
TATGCCG172800.018.5186148-49
TCACGTG175450.018.49550230-31
GTCACGT172850.018.12712528-29
CGGAAGA346100.018.0528624
ACGTCTG173850.018.01212314-15
TCGTATG176000.017.70752144-45
TCGGAAG351650.017.525283
CGTCTGA188800.017.37804416-17
TGAAACG179150.017.31977734-35
CACACGT192150.017.0874412-13
GATCGGA360200.016.9266031
ACACGTC187600.016.8564712-13
CTCGTAT180250.016.57837544-45
GCACACG198650.015.86962810-11
Produced by FastQC (version 0.11.2)