FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 kx12-3.3410000000cd6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 kx12-3.3410000000cd6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21188867
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA61850.012.59034514-15
CGCGTAT17350.012.3242441
CTCGTTT88850.011.9794771
CTCGGAT109650.011.35376551
CTCGGGT114150.011.155941
CTCGGTT94850.010.8710031
CTGGAAT194900.010.7760021
CTCGCAT83450.010.7617451
CGGAAAT66100.010.5672781
AGTCACG68950.010.54217128-29
CTCGATT112800.09.9835841
CGCGAAT29550.09.9696861
CTCGTGT83750.09.87214851
CCGGAAT80900.09.867521
CTTGAAT171150.09.8559551
CGCAAAT54550.09.8430791
CTCCCGT107250.09.6584281
GAGTGTC87000.09.57836524-25
CTCGACT117400.09.5114561
CTGGGAT152550.09.2199111
Produced by FastQC (version 0.11.2)