##FastQC	0.11.2
>>Basic Statistics	pass
#Measure	Value
Filename	C5B16ACXX l08n01 kx0-3.3410000000cb50.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	19951310
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76899782520546	33.0	33.0	33.0	33.0	33.0
2	32.76821456836669	33.0	33.0	33.0	33.0	33.0
3	32.60952358516809	33.0	33.0	33.0	33.0	33.0
4	36.60026459415447	37.0	37.0	37.0	37.0	37.0
5	36.65725147872496	37.0	37.0	37.0	37.0	37.0
6	36.601456646205186	37.0	37.0	37.0	37.0	37.0
7	36.62209829830723	37.0	37.0	37.0	37.0	37.0
8	36.621897860341	37.0	37.0	37.0	37.0	37.0
9	36.622526089765536	37.0	37.0	37.0	37.0	37.0
10-11	36.59920371143549	37.0	37.0	37.0	37.0	37.0
12-13	36.59996671897735	37.0	37.0	37.0	37.0	37.0
14-15	38.897837234747996	40.0	40.0	40.0	37.0	40.0
16-17	38.889414680038556	40.0	40.0	40.0	37.0	40.0
18-19	38.86361013387091	40.0	40.0	40.0	37.0	40.0
20-21	38.851784118436335	40.0	40.0	40.0	37.0	40.0
22-23	38.81280194132616	40.0	40.0	40.0	37.0	40.0
24-25	38.79809270669445	40.0	40.0	40.0	37.0	40.0
26-27	38.74139931162415	40.0	37.0	40.0	37.0	40.0
28-29	38.69923000043606	40.0	37.0	40.0	37.0	40.0
30-31	38.63702862117826	40.0	37.0	40.0	37.0	40.0
32-33	38.56406045517813	40.0	37.0	40.0	37.0	40.0
34-35	38.494920158124955	40.0	37.0	40.0	37.0	40.0
36-37	38.43206002513118	40.0	37.0	40.0	37.0	40.0
38-39	38.3424742285093	40.0	37.0	40.0	37.0	40.0
40-41	38.204747257197646	40.0	37.0	40.0	37.0	40.0
42-43	38.09227389078712	40.0	37.0	40.0	37.0	40.0
44-45	38.04957864922153	40.0	37.0	40.0	35.0	40.0
46-47	37.93626791924942	40.0	37.0	40.0	33.0	40.0
48-49	37.831044903818345	40.0	37.0	40.0	33.0	40.0
50-51	37.71146142784609	40.0	37.0	40.0	33.0	40.0
52-53	37.53575727608864	40.0	37.0	40.0	33.0	40.0
54-55	37.37569330033968	40.0	37.0	40.0	33.0	40.0
56-57	37.23703115234037	37.0	37.0	40.0	33.0	40.0
58-59	37.07210336063146	37.0	37.0	40.0	33.0	40.0
60-61	36.875214359357855	37.0	37.0	40.0	33.0	40.0
62-63	36.67404300770225	37.0	37.0	40.0	33.0	40.0
64-65	36.43463251285254	37.0	37.0	40.0	33.0	40.0
66-67	36.225133988695475	37.0	37.0	40.0	33.0	40.0
68-69	35.99357964464488	37.0	37.0	38.5	33.0	40.0
70-71	35.644712151733394	37.0	37.0	37.0	33.0	40.0
72-73	35.3105402352026	37.0	35.0	37.0	33.0	40.0
74-75	35.03496715253284	37.0	33.0	37.0	33.0	38.5
76-77	32.42271329551794	33.0	30.0	37.0	24.5	37.0
78-79	34.48472391035977	37.0	33.0	37.0	27.0	37.0
80-81	34.68974972069503	37.0	33.0	37.0	33.0	37.0
82-83	34.634603241591655	37.0	33.0	37.0	33.0	37.0
84-85	34.51830739435155	37.0	33.0	37.0	30.0	37.0
86-87	34.443714222274124	37.0	33.0	37.0	27.0	37.0
88-89	34.35015460137706	37.0	33.0	37.0	27.0	37.0
90-91	34.27251629091022	37.0	33.0	37.0	27.0	37.0
92-93	34.18596104716933	37.0	33.0	37.0	27.0	37.0
94-95	34.11127329483628	37.0	33.0	37.0	27.0	37.0
96-97	34.04586819111126	37.0	33.0	37.0	27.0	37.0
98-99	33.994740821530016	37.0	33.0	37.0	27.0	37.0
100-101	32.11662988545614	35.0	30.0	37.0	21.0	37.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
2306	1	0.09037449809675735
2306	2	0.1187251416309465
2306	3	0.24808556514136626
2306	4	0.2543382235474354
2306	5	0.20999604474702238
2306	6	0.2048180406554465
2306	7	0.19410359347856598
2306	8	0.20383508208503542
2306	9	0.2142684697690882
2306	10-11	0.22373733370030635
2306	12-13	0.22300431638761609
2306	14-15	0.45117998699436157
2306	16-17	0.46856779375888635
2306	18-19	0.43726913717767246
2306	20-21	0.4520539238197827
2306	22-23	0.47271079903778457
2306	24-25	0.4755973695146949
2306	26-27	0.4915728080385051
2306	28-29	0.4939976014417127
2306	30-31	0.5329163689680954
2306	32-33	0.5281025301571347
2306	34-35	0.5053998888803832
2306	36-37	0.5045380998930966
2306	38-39	0.5433693820723846
2306	40-41	0.5429261791496813
2306	42-43	0.568455693500411
2306	44-45	0.5382705952397515
2306	46-47	0.5501082491272058
2306	48-49	0.5490298599919115
2306	50-51	0.5416161691519576
2306	52-53	0.5142653312543572
2306	54-55	0.5413258567229065
2306	56-57	0.5358189075590758
2306	58-59	0.5517623799264655
2306	60-61	0.5605347846127415
2306	62-63	0.5916982120703764
2306	64-65	0.5468583875185473
2306	66-67	0.5594814102936425
2306	68-69	0.5684382081087236
2306	70-71	0.5059117047577217
2306	72-73	0.41895600941661115
2306	74-75	0.4832031985086047
2306	76-77	0.3269795569988716
2306	78-79	0.5078549045267451
2306	80-81	0.49866518647223046
2306	82-83	0.49564018589153136
2306	84-85	0.5333896921305765
2306	86-87	0.5410841835616438
2306	88-89	0.48585922615703936
2306	90-91	0.5486245791708697
2306	92-93	0.566911870389049
2306	94-95	0.5638806854017773
2306	96-97	0.5990925937652847
2306	98-99	0.4827814854034571
2306	100-101	0.31550428587456736
2307	1	0.04993757047868286
2307	2	0.08617577436302071
2307	3	0.23978342756225857
2307	4	0.22330769069642287
2307	5	0.1913394809438529
2307	6	0.1673061892113452
2307	7	0.17878867977473334
2307	8	0.19392657071469444
2307	9	0.1959230543206658
2307	10-11	0.20436279990264694
2307	12-13	0.19264414808503005
2307	14-15	0.4564448235521539
2307	16-17	0.4402896753867296
2307	18-19	0.43188063154940437
2307	20-21	0.438500895030991
2307	22-23	0.45120894284573865
2307	24-25	0.4583347625311731
2307	26-27	0.5014352797931352
2307	28-29	0.47134242187460273
2307	30-31	0.46979055964923333
2307	32-33	0.4889833696271566
2307	34-35	0.4804706717682308
2307	36-37	0.5067251970727398
2307	38-39	0.49085050751136805
2307	40-41	0.48737645604943225
2307	42-43	0.4633899801196648
2307	44-45	0.44256874699245685
2307	46-47	0.4650224678966097
2307	48-49	0.47647754181686963
2307	50-51	0.5186424954890754
2307	52-53	0.516556740058185
2307	54-55	0.5076723583741725
2307	56-57	0.5203267397583886
2307	58-59	0.532702746171033
2307	60-61	0.5304013464423392
2307	62-63	0.4882544362039667
2307	64-65	0.5344740461582873
2307	66-67	0.5336907237684656
2307	68-69	0.5541777077450121
2307	70-71	0.5284744162630375
2307	72-73	0.44437672911427484
2307	74-75	0.41134331363181786
2307	76-77	0.42661547517131737
2307	78-79	0.5504110206285446
2307	80-81	0.505109681779885
2307	82-83	0.44402300892180335
2307	84-85	0.5109473078564761
2307	86-87	0.5337386737430521
2307	88-89	0.4986168026215765
2307	90-91	0.4591886375004677
2307	92-93	0.49279648571707924
2307	94-95	0.5214801224594936
2307	96-97	0.49004219945978633
2307	98-99	0.4325758782175413
2307	100-101	0.31361149123731735
2201	1	0.07122066524689785
2201	2	0.09689350244004657
2201	3	0.22712143693684084
2201	4	0.2160272167315611
2201	5	0.18409964293260117
2201	6	0.1735642573327283
2201	7	0.16362809368688858
2201	8	0.15804783650120413
2201	9	0.17841632791709117
2201	10-11	0.18603971650575346
2201	12-13	0.18120312066110245
2201	14-15	0.3813682490636552
2201	16-17	0.38115436797490077
2201	18-19	0.3710989210664124
2201	20-21	0.39680198400704825
2201	22-23	0.3809388872118049
2201	24-25	0.38869680506260096
2201	26-27	0.38796568006195997
2201	28-29	0.44625688384858364
2201	30-31	0.42924291000878867
2201	32-33	0.4653424144365914
2201	34-35	0.4408876525337604
2201	36-37	0.40958976927197455
2201	38-39	0.4701423355404586
2201	40-41	0.45963333228285563
2201	42-43	0.46173661061714455
2201	44-45	0.4718513677058098
2201	46-47	0.4665156844864313
2201	48-49	0.5066478996607842
2201	50-51	0.4928090458922867
2201	52-53	0.4976106532986435
2201	54-55	0.5322686803984524
2201	56-57	0.5043338010757381
2201	58-59	0.47922556868773825
2201	60-61	0.4762680023827599
2201	62-63	0.5036939983332829
2201	64-65	0.48211581069253384
2201	66-67	0.4660162404470185
2201	68-69	0.44001297442712684
2201	70-71	0.4289257059998306
2201	72-73	0.4042843821751134
2201	74-75	0.374149461568436
2201	76-77	0.3374454068609083
2201	78-79	0.5710245035141881
2201	80-81	0.5592244893188578
2201	82-83	0.5261570565628162
2201	84-85	0.5166910883240732
2201	86-87	0.5141517409305791
2201	88-89	0.5302239423980097
2201	90-91	0.5700391684494264
2201	92-93	0.5642969556464763
2201	94-95	0.6205637038840379
2201	96-97	0.5317309749355488
2201	98-99	0.5239710603348655
2201	100-101	0.4739024761688242
1101	1	0.05301808259599028
1101	2	0.08200155232140105
1101	3	0.22335769885778944
1101	4	0.20744701941046628
1101	5	0.1518836744526837
1101	6	0.1737610997416965
1101	7	0.12905115575551918
1101	8	0.1290249021388945
1101	9	0.14810295676471696
1101	10-11	0.13124366388708353
1101	12-13	0.16194976046495668
1101	14-15	0.34230087536718656
1101	16-17	0.3323351716393077
1101	18-19	0.3398976900612567
1101	20-21	0.31938354834836247
1101	22-23	0.3418884036353447
1101	24-25	0.3557021655221817
1101	26-27	0.36223511694819877
1101	28-29	0.36764340404987905
1101	30-31	0.3645637219732336
1101	32-33	0.3285138784861559
1101	34-35	0.36947422971876875
1101	36-37	0.38065398149561247
1101	38-39	0.37910007447568717
1101	40-41	0.37070362287797565
1101	42-43	0.3732403443259287
1101	44-45	0.3883087288213787
1101	46-47	0.38167139416479046
1101	48-49	0.4392614974395386
1101	50-51	0.44227010767784947
1101	52-53	0.4169179631986495
1101	54-55	0.3874060355648581
1101	56-57	0.4399463628762419
1101	58-59	0.40300224531837614
1101	60-61	0.3944478149929296
1101	62-63	0.40045222929547464
1101	64-65	0.41381159836856796
1101	66-67	0.43237323674035366
1101	68-69	0.3821915131362559
1101	70-71	0.42872065841635276
1101	72-73	0.3719695645131722
1101	74-75	0.37942447557448133
1101	76-77	0.3079227173998831
1101	78-79	0.4365598946943905
1101	80-81	0.37458924830698237
1101	82-83	0.4390304547001307
1101	84-85	0.4177532004591953
1101	86-87	0.4391472567073009
1101	88-89	0.4166345541910843
1101	90-91	0.5240580465660045
1101	92-93	0.4906456573534399
1101	94-95	0.40410436371310254
1101	96-97	0.4130441561422131
1101	98-99	0.40701082560908475
1101	100-101	0.5207507132611866
2304	1	0.05638646911084777
2304	2	0.08769636255297542
2304	3	0.23347207143517323
2304	4	0.21420642043479177
2304	5	0.1774507825708156
2304	6	0.2069728287485546
2304	7	0.182546387930131
2304	8	0.18926398184269289
2304	9	0.21062442373801105
2304	10-11	0.19351204411002954
2304	12-13	0.18240211528897277
2304	14-15	0.3694680716946124
2304	16-17	0.3972491458992451
2304	18-19	0.4208168061413886
2304	20-21	0.4110104250977642
2304	22-23	0.4362504963078919
2304	24-25	0.40766169090048976
2304	26-27	0.44918458780973936
2304	28-29	0.4356773077100158
2304	30-31	0.46507322811803675
2304	32-33	0.4964845486055154
2304	34-35	0.4874300468571491
2304	36-37	0.5077424456954205
2304	38-39	0.5376502162679628
2304	40-41	0.5482227229572558
2304	42-43	0.45544183769958835
2304	44-45	0.4839254874413186
2304	46-47	0.48467440994761546
2304	48-49	0.527979755001418
2304	50-51	0.5210284265580682
2304	52-53	0.4905225467899683
2304	54-55	0.54978840886222
2304	56-57	0.5975169408051642
2304	58-59	0.5976521446969159
2304	60-61	0.6435031063330143
2304	62-63	0.5951427087889911
2304	64-65	0.6509117485548686
2304	66-67	0.6184949455043167
2304	68-69	0.6098784927061089
2304	70-71	0.5785404314967053
2304	72-73	0.5885082166478597
2304	74-75	0.5523094548365464
2304	76-77	0.7397626865116038
2304	78-79	0.6551115251370518
2304	80-81	0.5871830029088088
2304	82-83	0.5647806334092635
2304	84-85	0.5600275112396744
2304	86-87	0.4623176902886925
2304	88-89	0.5504128939625801
2304	90-91	0.6109081825541836
2304	92-93	0.6429521805725287
2304	94-95	0.5523106798609021
2304	96-97	0.5367126258159729
2304	98-99	0.6508597766301634
2304	100-101	0.7746599753149468
2202	1	0.06370121677863239
2202	2	0.06291206566768182
2202	3	0.2012445122091222
2202	4	0.1606060567980876
2202	5	0.1176690404324674
2202	6	0.13351194765946417
2202	7	0.08578191095176635
2202	8	0.0675872431217357
2202	9	0.10554975782212495
2202	10-11	0.10380408154679088
2202	12-13	0.08345847234306092
2202	14-15	0.23360079909316056
2202	16-17	0.23271668354977493
2202	18-19	0.24897541658656763
2202	20-21	0.21567920381497174
2202	22-23	0.25129526362684373
2202	24-25	0.22797157838734705
2202	26-27	0.21518696339261822
2202	28-29	0.2478327462875498
2202	30-31	0.2524922796101663
2202	32-33	0.2721086036098299
2202	34-35	0.2547878081214989
2202	36-37	0.2729420145640944
2202	38-39	0.26681087999026687
2202	40-41	0.23753343421966377
2202	42-43	0.24241140540227235
2202	44-45	0.26322805100426905
2202	46-47	0.26905471555033245
2202	48-49	0.31507820708070966
2202	50-51	0.280689967615551
2202	52-53	0.3055268505195272
2202	54-55	0.2984084947661714
2202	56-57	0.3035893941869574
2202	58-59	0.3088950788545972
2202	60-61	0.24858639392737558
2202	62-63	0.27303301866699314
2202	64-65	0.2925536024195594
2202	66-67	0.26888924865561137
2202	68-69	0.23579708301664226
2202	70-71	0.2507340111664149
2202	72-73	0.22732880515918197
2202	74-75	0.31544714384752837
2202	76-77	0.30495291799348223
2202	78-79	0.3398472676109776
2202	80-81	0.3400281805040066
2202	82-83	0.2708194579792078
2202	84-85	0.30160779522894643
2202	86-87	0.33980507533692617
2202	88-89	0.3343201921968202
2202	90-91	0.39658726652336185
2202	92-93	0.3869706505871875
2202	94-95	0.3880606189545901
2202	96-97	0.3354366458406375
2202	98-99	0.34466651073456234
2202	100-101	0.46217598869631615
1102	1	0.017385343433268474
1102	2	0.05188153427765485
1102	3	0.18763406682951
1102	4	0.13521824461194853
1102	5	0.11568484605145102
1102	6	0.11303910006898832
1102	7	0.09469485004624545
1102	8	0.09528014275458219
1102	9	0.07578256465095734
1102	10-11	0.08261450847463436
1102	12-13	0.0887351545469599
1102	14-15	0.18111914078221503
1102	16-17	0.20101587062346482
1102	18-19	0.20614447464463836
1102	20-21	0.21648806234231444
1102	22-23	0.22401293685857127
1102	24-25	0.2069900699970333
1102	26-27	0.23608392912473875
1102	28-29	0.20752818723244815
1102	30-31	0.17006440401703316
1102	32-33	0.16960065152571957
1102	34-35	0.19359296161717054
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1213	76-77	-0.19001515303708771
1213	78-79	-0.5698178259684639
1213	80-81	-0.6411184484054715
1213	82-83	-0.6208208728740701
1213	84-85	-0.9814894589396559
1213	86-87	-0.7866782466688633
1213	88-89	-0.7981473675949076
1213	90-91	-0.7115743089396531
1213	92-93	-0.6913998392173397
1213	94-95	-0.6132819658186719
1213	96-97	-0.5314080518919369
1213	98-99	-0.5014731359195892
1213	100-101	-0.15910960044332256
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	10.0
8	146.0
9	505.0
10	639.0
11	442.0
12	414.0
13	484.0
14	750.0
15	1599.0
16	3463.0
17	5704.0
18	8913.0
19	13132.0
20	20034.0
21	27648.0
22	38213.0
23	49935.0
24	65920.0
25	85960.0
26	112471.0
27	143870.0
28	192452.0
29	266533.0
30	291246.0
31	318023.0
32	406160.0
33	547071.0
34	766967.0
35	1207514.0
36	2446996.0
37	6218168.0
38	6705665.0
39	4262.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.914477247957954	13.224254030606591	12.83526982728684	52.025998894148614
2	22.334320001656227	19.00171903758558	39.22436430623703	19.439596654521168
3	23.745094737952208	23.55507198745071	27.023264115784208	25.676569158812878
4	26.08213195023284	28.07380066772558	19.688040534681683	26.156026847359897
5	28.725910825205826	29.51789722145896	22.6815593063276	19.07463264700761
6	21.502823624112903	32.62801790960093	23.77048925609396	22.098669210192213
7	18.703162849958225	15.810159834116156	43.1680476119112	22.318629704014423
8	20.820743099074697	21.54562783095446	28.65788261522677	28.975746454744073
9	22.026042400223346	20.267370914491327	32.05995997255318	25.646626712732147
10-11	25.082467656942665	29.147940762676367	22.1339063684877	23.635685211893268
12-13	22.300134171728285	23.565397778400072	28.92237281616328	25.212095233708364
14-15	23.059091198773494	25.353389799649438	27.06259883908844	24.524920162488623
16-17	24.648121628732913	25.088467371364874	25.729540982142325	24.533870017759888
18-19	23.863260719793068	25.24932715039326	26.175033877435737	24.71237825237794
20-21	24.209783718462596	25.39246295105434	26.421047039016486	23.976706291466577
22-23	23.663280757002923	26.566481098233652	25.74087616301887	24.029361981744557
24-25	23.43359709652896	25.630377079580178	26.29435912485369	24.641666699037167
26-27	23.582230103883536	25.51168695734723	25.980337108429346	24.92574583033989
28-29	24.2082597547015	25.861023179026443	25.598675044558185	24.332042021713875
30-31	23.63338620354626	25.422910322003005	26.319267711208028	24.624435763242705
32-33	23.727431032213175	26.102743667374995	25.68395860365697	24.48586669675486
34-35	24.531943631029346	25.838442544041563	25.485560446256066	24.144053378673025
36-37	23.66140289772861	25.587704984189585	26.126793259455876	24.624098858625928
38-39	24.38555943482191	26.13663472257758	25.668262930937765	23.80954291166275
40-41	24.104241634619704	25.939725966384398	26.02088997727287	23.935142421723025
42-43	23.57540595457638	25.39302382260365	26.345478595746975	24.686091627072997
44-45	24.315900421048386	25.479525101787043	25.861776221853177	24.342798255311397
46-47	24.11384580379235	25.831213375606637	26.068212256533695	23.986728564067313
48-49	24.20459439190178	25.45931311852495	26.16119386195971	24.174898627613562
50-51	23.981356520260178	25.463947835481715	25.716655540705062	24.83804010355305
52-53	24.62606791125847	25.339456867507447	25.92733480448674	24.107140416747335
54-55	23.63911651149525	25.42244418506691	26.24461368760307	24.69382561583477
56-57	23.798180492160903	25.487639313339983	26.456411602872638	24.257768591626476
58-59	24.457647757105626	25.368261949426156	26.04919038686197	24.124899906606245
60-61	23.81180994856549	25.374723823968264	26.104962387705466	24.708503839760784
62-63	24.379894481807764	25.573221612102692	26.389406080430465	23.657477825659075
64-65	23.97775042879552	26.629785328655796	25.529258225754752	23.863206016793928
66-67	23.70631737192675	26.74178388691506	25.519977599198622	24.031921141959565
68-69	23.94081200983198	26.77591117162092	25.57911344908133	23.704163369465764
70-71	24.034378583244635	26.642805120647818	25.403317225556695	23.91949907055086
72-73	23.865199058182185	26.563996682010366	25.5138794012759	24.05692485853155
74-75	23.991259572990117	26.603611399975257	25.58484521264115	23.820283814393477
76-77	24.044353468250417	26.42887543353475	25.49695213984936	24.029818958365475
78-79	23.903284209579766	26.34886986729396	25.594173247776336	24.15367267534993
80-81	24.052961583755053	26.420060783722572	25.53250164840824	23.994475984114132
82-83	24.11953271110484	26.39035528455806	25.40485636161538	24.085255642721723
84-85	23.891098105465034	26.3615888464506	25.59572719287946	24.151585855204914
86-87	24.110345923365138	26.499907837473675	25.6311616851495	23.758584554011687
88-89	24.15069315295678	26.247351531380243	25.36589616479289	24.236059150870084
90-91	24.07861867226798	26.189000255701	25.450567442949644	24.281813629081377
92-93	24.139532273856727	26.390333424798445	25.532487729210267	23.93764657213456
94-95	24.229757440881468	26.331415852676578	25.395761546643037	24.043065159798925
96-97	23.987414861480275	26.374784412652602	25.475226939985397	24.16257378588173
98-99	24.230662403178783	26.444308396299874	25.428515943383157	23.896513257138185
100-101	24.50975564212156	26.327787139346835	25.072767998816314	24.08968921971529
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	1.0
4	2.0
5	3.5
6	5.5
7	7.5
8	12.0
9	19.0
10	29.5
11	53.0
12	80.0
13	121.5
14	172.5
15	255.0
16	393.0
17	574.5
18	860.0
19	1262.5
20	1943.5
21	3045.0
22	4756.5
23	7491.5
24	11482.5
25	17140.0
26	25063.5
27	35544.0
28	48664.5
29	65389.5
30	86102.0
31	111481.0
32	141293.5
33	177240.0
34	223698.0
35	282579.0
36	354449.5
37	439108.5
38	534664.0
39	634753.0
40	731215.5
41	818357.5
42	894370.5
43	936102.0
44	943712.5
45	925663.5
46	882414.0
47	826605.0
48	766025.5
49	709660.5
50	652962.0
51	600584.0
52	559027.0
53	528800.0
54	510470.0
55	492952.0
56	478689.5
57	457888.0
58	422454.0
59	398344.0
60	377173.5
61	346953.5
62	327018.5
63	305118.0
64	266464.0
65	235638.5
66	217504.0
67	201876.0
68	186646.0
69	160752.5
70	132251.0
71	108713.0
72	87604.0
73	71971.5
74	55280.5
75	39259.0
76	28080.0
77	19926.5
78	13372.5
79	8721.0
80	5670.0
81	3503.5
82	2186.5
83	1247.0
84	727.5
85	501.5
86	367.0
87	246.5
88	168.0
89	123.0
90	76.5
91	40.5
92	25.5
93	22.0
94	18.0
95	10.5
96	8.0
97	5.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.07788461008324767
2	0.01181376060018114
3	0.0027116013935927015
4	0.0
5	0.0016840999413071122
6	0.0
7	0.0
8	0.0
9	0.0
10-11	1.7542707721949084E-5
12-13	6.515862868152516E-5
14-15	2.7567112134491416E-5
16-17	8.470621728598272E-4
18-19	0.0035837245774838844
20-21	0.0
22-23	0.0
24-25	8.771353860974543E-5
26-27	3.683968621609308E-4
28-29	7.443120276312684E-4
30-31	0.0123250052252208
32-33	0.04979622891930404
34-35	0.044175044144970935
36-37	0.04943785646155565
38-39	0.04206490701613077
40-41	0.06967963507158177
42-43	0.06220393548092832
44-45	0.0460320650623944
46-47	0.03570191631526952
48-49	0.011713516556055717
50-51	0.038671646122485186
52-53	0.05827436895121173
54-55	0.05430971700605123
56-57	0.046182431128582536
58-59	0.049791216717097776
60-61	0.05124475535691641
62-63	0.06631895349227696
64-65	0.061745318979054505
66-67	0.02804327134408718
68-69	0.0667249418709849
70-71	0.07403022658662514
72-73	0.09099903715595617
74-75	0.05101920625763422
76-77	0.028454271925001412
78-79	0.0035611696675556643
80-81	0.001227989540536436
82-83	0.0032779802429013434
84-85	0.007154918649452091
86-87	7.142388143936414E-4
88-89	7.919279485908444E-4
90-91	0.0013708373034151643
92-93	8.495682739629629E-4
94-95	0.002834400347646345
96-97	0.0
98-99	1.979819871477111E-4
100-101	3.032382334794056E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1.995131E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.7362762877518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.7283048974977	36.6697134260955
2	13.351805698591201	13.281381943310217
3	4.830017025815625	7.2068118381153345
4	2.5677587917510074	5.108430428273273
5	1.5310932415006318	3.807543824079245
6	0.8398039737361905	2.5061233479177014
7	0.5576312544505192	1.9414151496625651
8	0.4540508095004475	1.8066237207993292
9	0.2855991933050725	1.2784176347202107
>10	1.6909174278862127	14.988255700073902
>50	0.10079081332553827	3.4487462748694853
>100	0.058502412971162196	5.168398511020348
>500	0.003046342935874386	1.0312557661328974
>1k	6.350894978588153E-4	0.5943179124531266
>5k	2.2781622169561243E-5	0.060618228949912145
>10k+	2.0245612499774966E-5	1.1019462935270838
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC	173204	0.8681334709349913	TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG	45666	0.22888722595157912	TruSeq Adapter, Index 9 (100% over 49bp)
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA Adapter	Nextera Transposase Sequence
1	0.2524846739387038	6.515862868152518E-5	5.012202206271167E-6
2	0.2538680417476346	6.515862868152518E-5	1.5036606618813502E-5
3	0.2548253723690324	7.017083088779634E-5	1.5036606618813502E-5
4	0.25707585115964815	7.017083088779634E-5	1.5036606618813502E-5
5	0.25753196156041885	7.518303309406751E-5	2.004880882508467E-5
6	0.25881508532522424	1.0024404412542334E-4	3.508541544389817E-5
7	0.25911581745760054	1.0024404412542334E-4	4.009761765016934E-5
8	0.26025859956063035	1.0024404412542334E-4	4.510981985644051E-5
9	0.2612760766085034	1.0525624633169451E-4	4.510981985644051E-5
10-11	0.2634363357594063	1.1277454964110127E-4	4.510981985644051E-5
12-13	0.26511041129630086	1.3532945956932151E-4	5.012202206271167E-5
14-15	0.2670000115280651	1.528721672912706E-4	5.012202206271167E-5
16-17	0.26900489241057357	1.6540267280694851E-4	5.012202206271167E-5
18-19	0.27071906556511827	1.6540267280694851E-4	5.262812316584726E-5
20-21	0.27308733110758143	1.7292097611635524E-4	6.014642647525401E-5
22-23	0.2754881759643853	2.004880882508467E-4	6.515862868152518E-5
24-25	0.27647307369791757	2.155246948696602E-4	7.76891341972031E-5
26-27	0.27737527009504637	2.1803079597279578E-4	9.523184191915218E-5
28-29	0.27822483836900935	2.2053689707593135E-4	9.523184191915218E-5
30-31	0.27906939444076606	2.2554909928220251E-4	1.0525624633169451E-4
32-33	0.2798412735805318	2.2554909928220251E-4	1.1778675184737243E-4
34-35	0.28041517073314987	2.3807960479788043E-4	1.7292097611635527E-4
36-37	0.2810442021100369	2.4058570590101603E-4	1.8043927942576203E-4
38-39	0.2817935263398744	2.4058570590101603E-4	1.8795758273516876E-4
40-41	0.2825503688730214	2.606345147261007E-4	2.2304299817906692E-4
42-43	0.2832696198896213	2.7316502024177865E-4	2.681528180355074E-4
44-45	0.28398887090622116	2.8068332355118535E-4	2.882016268605921E-4
46-47	0.284650481597449	2.907077279637277E-4	2.932138290668633E-4
48-49	0.2854875193658963	3.057443345825412E-4	2.9822603127313443E-4
50-51	0.2872167291270598	3.182748400982191E-4	3.157687389950835E-4
52-53	0.28906372564007077	3.358175478201682E-4	3.182748400982191E-4
54-55	0.29089067334425656	3.5586635664525287E-4	3.257931434076259E-4
56-57	0.29202092494177073	3.658907610577952E-4	3.383236489233038E-4
58-59	0.2934644391771768	3.6839686216093076E-4	3.43335851129575E-4
60-61	0.2954843566663041	3.859395698828799E-4	3.483480533358461E-4
62-63	0.29770476224368225	4.03482277604829E-4	3.633846599546596E-4
64-65	0.30119826718145326	4.1851888422364243E-4	3.658907610577952E-4
66-67	0.3045689731651706	4.235310864299136E-4	3.6839686216093076E-4
68-69	0.30806247810294163	4.285432886361848E-4	3.8844567098601546E-4
70-71	0.31296691796177795	4.536042996675406E-4	4.034822776048289E-4
72-73	0.3196331468961186	4.561104007706762E-4	4.1100058091423573E-4
74-75	0.32906109924611465	4.586165018738118E-4	4.1851888422364243E-4
76-77	0.3397095228333378	4.686409062863541E-4	4.3355549084245595E-4
78-79	0.35269112654758006	4.736531084926253E-4	4.561104007706762E-4
80-81	0.3698654374073682	4.886897151114388E-4	4.8618361400830324E-4
82-83	0.39485126540563	4.962080184208455E-4	5.012202206271168E-4
84-85	0.4291597895075562	5.012202206271168E-4	5.062324228333879E-4
86-87	0.4716757947222513	5.137507261427946E-4	5.087385239365234E-4
88-89	0.5227451230019482	5.262812316584725E-4	5.11244625039659E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAGCAC	41590	0.0	44.918007	8
GAGCACA	43075	0.0	43.865433	9
ATCGGAA	43090	0.0	42.865345	2
TCGGAAG	43985	0.0	42.095913	3
CGGAAGA	45645	0.0	41.021507	4
GATCGGA	46685	0.0	39.595943	1
AGTCACG	29495	0.0	39.457256	28-29
TATGCCG	30365	0.0	37.95605	46-47
CGTATGC	30690	0.0	37.623905	44-45
CGTCTGA	31475	0.0	37.123566	16-17
CACACGT	32150	0.0	36.366253	12-13
CTCGTAT	31045	0.0	36.266613	42-43
CACGTCT	32635	0.0	35.789474	14-15
ACGATCA	35135	0.0	33.160107	32-33
AAGAGCA	57040	0.0	32.917862	7
TCACGAT	36485	0.0	32.01638	30-31
AGCACAC	37275	0.0	31.631308	10-11
CCAGTCA	36955	0.0	31.585308	26-27
CCGTCTT	37550	0.0	30.685976	50-51
GAAGAGC	61190	0.0	30.661968	6
>>END_MODULE