FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 kx0-3.3410000000cb50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 kx0-3.3410000000cb50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19951310
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1732040.8681334709349913TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG456660.22888722595157912TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC415900.044.9180078
GAGCACA430750.043.8654339
ATCGGAA430900.042.8653452
TCGGAAG439850.042.0959133
CGGAAGA456450.041.0215074
GATCGGA466850.039.5959431
AGTCACG294950.039.45725628-29
TATGCCG303650.037.9560546-47
CGTATGC306900.037.62390544-45
CGTCTGA314750.037.12356616-17
CACACGT321500.036.36625312-13
CTCGTAT310450.036.26661342-43
CACGTCT326350.035.78947414-15
ACGATCA351350.033.16010732-33
AAGAGCA570400.032.9178627
TCACGAT364850.032.0163830-31
AGCACAC372750.031.63130810-11
CCAGTCA369550.031.58530826-27
CCGTCTT375500.030.68597650-51
GAAGAGC611900.030.6619686
Produced by FastQC (version 0.11.2)