FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 k48-2.3410000000ce40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 k48-2.3410000000ce40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12586605
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTA301880.23984227676962933TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT251540.19984737743021252TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG84700.034.6940728-29
CGTATGC87550.033.3733546-47
TATGCCG87200.033.26661748-49
ACGTGGC89650.032.72817632-33
TCACGTG90150.032.46617530-31
CACACGT93000.031.73003812-13
CTCGTAT92600.031.245144-45
CACGTCT98600.030.0724514-15
AGATCGG110500.028.5112761
CGCGTAT33150.025.3720251
AGCACAC118000.025.26650610-11
ACATGCG41000.024.779834
GGCCTTA118750.024.71203236-37
CGTCTGA121400.024.62012916-17
ATCGGAA129250.024.427083
TTATCTC123800.023.60778640-41
CCAGTCA127650.023.29805426-27
CCTTATC127150.022.87389838-39
CATGCGT44000.022.7666025
CCGTCTT132300.022.05327252-53
Produced by FastQC (version 0.11.2)