FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l08n01 k12-3.3410000000cc5f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l08n01 k12-3.3410000000cc5f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20792259
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT459580.22103418392393054TruSeq Adapter, Index 18 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTA299580.14408246838402697TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG131300.029.30897328-29
TATGCCG141000.027.26266348-49
CCGCACA139550.027.20953836-37
CGTATGC143850.026.8016246-47
CACACGT154300.025.49374812-13
CTCGTAT153100.024.99612844-45
CGTCTGA157900.024.77725816-17
GTCCGCA156400.024.44301834-35
ACGTCCG159900.024.00989332-33
TCACGTC164000.023.65474530-31
CACGTCT179550.021.88218714-15
AGCACAC198200.020.00112510-11
GAGCACA251450.019.5224939
AGAGCAC251250.019.4057648
ATCGGAA246550.019.1617722
CCAGTCA211150.018.4487126-27
CGCACAT133450.017.25358836-37
TCTGAAC231950.017.22437118-19
GCACATC222750.017.18505738-39
TCGGAAG281750.016.9854323
Produced by FastQC (version 0.11.2)