##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 mx48-3.3420000000cdd1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 14284593 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.004115833051735 33.0 33.0 33.0 33.0 33.0 2 31.513538677650807 33.0 33.0 33.0 27.0 33.0 3 31.562059696065543 33.0 33.0 33.0 27.0 33.0 4 35.42829970724402 37.0 37.0 37.0 33.0 37.0 5 35.60846094809982 37.0 37.0 37.0 33.0 37.0 6 35.233472735274994 37.0 37.0 37.0 33.0 37.0 7 35.55825636754229 37.0 37.0 37.0 33.0 37.0 8 35.31049866103991 37.0 37.0 37.0 33.0 37.0 9 35.101020519100544 37.0 37.0 37.0 33.0 37.0 10-11 35.35015281849472 37.0 37.0 37.0 33.0 37.0 12-13 35.63012106820264 37.0 37.0 37.0 33.0 37.0 14-15 37.60456692745814 40.0 37.0 40.0 33.0 40.0 16-17 37.66469622200647 40.0 37.0 40.0 33.0 40.0 18-19 37.72590738147037 40.0 37.0 40.0 33.0 40.0 20-21 37.53033163072969 40.0 37.0 40.0 33.0 40.0 22-23 37.45964921786711 40.0 37.0 40.0 33.0 40.0 24-25 37.57406479834602 40.0 37.0 40.0 33.0 40.0 26-27 37.569136306508696 40.0 37.0 40.0 33.0 40.0 28-29 37.39717995465464 40.0 37.0 40.0 33.0 40.0 30-31 37.37012027574045 40.0 37.0 40.0 33.0 40.0 32-33 37.36177359060913 40.0 37.0 40.0 33.0 40.0 34-35 37.14577401680258 40.0 37.0 40.0 33.0 40.0 36-37 37.18328600611862 40.0 37.0 40.0 33.0 40.0 38-39 37.1274456682105 40.0 37.0 40.0 33.0 40.0 40-41 37.03098387192411 40.0 37.0 40.0 33.0 40.0 42-43 36.9349814166914 40.0 37.0 40.0 33.0 40.0 44-45 36.81015766427507 40.0 37.0 40.0 33.0 40.0 46-47 36.65376342889154 40.0 37.0 40.0 33.0 40.0 48-49 36.506555349529386 38.5 37.0 40.0 33.0 40.0 50-51 35.06555311026362 37.0 35.0 38.5 30.0 38.5 52-53 35.40271721427415 37.0 35.0 38.5 27.0 40.0 54-55 36.08190943907188 37.0 37.0 40.0 33.0 40.0 56-57 36.03004618332493 37.0 37.0 40.0 33.0 40.0 58-59 35.89002735324696 37.0 37.0 40.0 30.0 40.0 60-61 35.71301856482715 37.0 37.0 40.0 27.0 40.0 62-63 35.534351556253654 37.0 37.0 40.0 27.0 40.0 64-65 35.37107816092485 37.0 33.0 38.5 27.0 40.0 66-67 35.13622176704649 37.0 33.0 37.0 27.0 40.0 68-69 34.935242537186745 37.0 33.0 37.0 27.0 40.0 70-71 34.71667673695708 37.0 33.0 37.0 27.0 40.0 72-73 34.503728177624666 37.0 33.0 37.0 27.0 40.0 74-75 34.30666834539843 37.0 33.0 37.0 27.0 37.0 76-77 34.11169558698663 37.0 33.0 37.0 27.0 37.0 78-79 33.95144712908517 37.0 33.0 37.0 27.0 37.0 80-81 33.83569776891788 37.0 33.0 37.0 27.0 37.0 82-83 33.73628706117143 37.0 33.0 37.0 27.0 37.0 84-85 33.62817859073759 37.0 33.0 37.0 27.0 37.0 86-87 33.52186873647713 37.0 33.0 37.0 27.0 37.0 88-89 33.42335588420335 37.0 33.0 37.0 27.0 37.0 90-91 33.32591124577368 37.0 33.0 37.0 27.0 37.0 92-93 33.243881922292076 37.0 33.0 37.0 27.0 37.0 94-95 33.162263146034334 37.0 33.0 37.0 27.0 37.0 96-97 33.07809546971342 37.0 33.0 37.0 27.0 37.0 98-99 32.99960782921852 37.0 33.0 37.0 27.0 37.0 100-101 31.207694681955587 35.0 30.0 35.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.49829843121916895 2306 2 0.8795664935284755 2306 3 0.8162946157817181 2306 4 0.8785320759751372 2306 5 0.7328780318986219 2306 6 0.9839041315800756 2306 7 0.7755241529126806 2306 8 0.9454959064605646 2306 9 1.0837175028499502 2306 10-11 0.9123349416031132 2306 12-13 0.6760339090784768 2306 14-15 1.0589269584103747 2306 16-17 0.9966489740307338 2306 18-19 0.9129123560200085 2306 20-21 0.9727948159864255 2306 22-23 1.1000084047687082 2306 24-25 1.0274776712208862 2306 26-27 0.912797566996197 2306 28-29 1.0344222064962523 2306 30-31 0.9697712621221299 2306 32-33 0.9583535292603997 2306 34-35 1.0599530537481314 2306 36-37 0.9810916053899064 2306 38-39 0.9662734523292613 2306 40-41 0.9299247806994799 2306 42-43 0.9451836012997532 2306 44-45 0.9668918280794472 2306 46-47 0.9323737881166352 2306 48-49 0.9417192592295365 2306 50-51 0.8878051977109749 2306 52-53 0.9172964389686626 2306 54-55 0.9151551316719733 2306 56-57 0.8712848355813918 2306 58-59 0.884660099629933 2306 60-61 0.8900379499078923 2306 62-63 0.9417912878418591 2306 64-65 0.9613216968386595 2306 66-67 0.9679041204627126 2306 68-69 0.9608079052410616 2306 70-71 0.8564465830165133 2306 72-73 0.9366651040997382 2306 74-75 0.8491120282260525 2306 76-77 0.905315886100162 2306 78-79 0.8680410747150304 2306 80-81 0.8514291411230985 2306 82-83 0.837860397449532 2306 84-85 0.8651603079300898 2306 86-87 0.7582975245866805 2306 88-89 0.8078292087456731 2306 90-91 0.8455650945903628 2306 92-93 0.8646800829436856 2306 94-95 0.9028035271688495 2306 96-97 0.8474654680058151 2306 98-99 0.8238412699962723 2306 100-101 0.7081260403429148 2307 1 0.32578171021383895 2307 2 0.7264397542474548 2307 3 0.6955184693547629 2307 4 0.6636331548685348 2307 5 0.5973865077354148 2307 6 0.8434145042972787 2307 7 0.6519345798141885 2307 8 0.8288714769725871 2307 9 0.9110788135716135 2307 10-11 0.7388985732110882 2307 12-13 0.4950079232942173 2307 14-15 0.8334960958096858 2307 16-17 0.7802185864360425 2307 18-19 0.6916937177612255 2307 20-21 0.8069754228856354 2307 22-23 0.9226064579379383 2307 24-25 0.7548869215156131 2307 26-27 0.5964765704043842 2307 28-29 0.8574222499120552 2307 30-31 0.7932630302596451 2307 32-33 0.7281077897174342 2307 34-35 0.7889818199181207 2307 36-37 0.7158063587467893 2307 38-39 0.6736019481101323 2307 40-41 0.6820624469689278 2307 42-43 0.6834373415082879 2307 44-45 0.6779211405200343 2307 46-47 0.5885312407363728 2307 48-49 0.6759815593829401 2307 50-51 0.5690373164848097 2307 52-53 0.7095377029368279 2307 54-55 0.7275027096818079 2307 56-57 0.7188343924376994 2307 58-59 0.7317322668127133 2307 60-61 0.7389846240667381 2307 62-63 0.7602103228147215 2307 64-65 0.7174169361988945 2307 66-67 0.6409603056291289 2307 68-69 0.6584387338435747 2307 70-71 0.6769879792230213 2307 72-73 0.6552109190506314 2307 74-75 0.607502055329725 2307 76-77 0.6165492310705218 2307 78-79 0.6363536975618445 2307 80-81 0.5804033850205741 2307 82-83 0.6225433149158945 2307 84-85 0.6042779648984222 2307 86-87 0.5976834909870519 2307 88-89 0.5384725865712809 2307 90-91 0.5131390466117338 2307 92-93 0.5604969119730683 2307 94-95 0.5318958471165232 2307 96-97 0.5778530145466974 2307 98-99 0.5741640681878621 2307 100-101 0.496352773358435 2201 1 0.38318540158558534 2201 2 0.7628410913236259 2201 3 0.723493387638019 2201 4 0.7620268608397893 2201 5 0.6208514456254619 2201 6 0.9427887937524133 2201 7 0.706986004648499 2201 8 0.9025729895650443 2201 9 1.0716314351474452 2201 10-11 0.8295460545676434 2201 12-13 0.6096100638875086 2201 14-15 0.8966676676579297 2201 16-17 0.8688142689357576 2201 18-19 0.8512986950912804 2201 20-21 0.9771553499360053 2201 22-23 1.0399204709821461 2201 24-25 0.8733498281889283 2201 26-27 0.8463915553847059 2201 28-29 0.9709774234624291 2201 30-31 0.9736712286248306 2201 32-33 0.9432028389042273 2201 34-35 0.9962807196648384 2201 36-37 0.8904029173039589 2201 38-39 0.8915521782929261 2201 40-41 0.8286905749447229 2201 42-43 0.8798272619708101 2201 44-45 0.8154874642093333 2201 46-47 0.8223749951041412 2201 48-49 0.8906848166882426 2201 50-51 0.5118500705229678 2201 52-53 0.6714154398000005 2201 54-55 0.8534583242217408 2201 56-57 0.8610711038608088 2201 58-59 0.8937304615770074 2201 60-61 0.923292096723678 2201 62-63 0.8677814447909142 2201 64-65 0.900222727931471 2201 66-67 0.8792825815672032 2201 68-69 0.8645086103116384 2201 70-71 0.894508418749588 2201 72-73 0.8685344293937263 2201 74-75 0.9411735764519804 2201 76-77 0.9063182688833678 2201 78-79 0.9154597488460325 2201 80-81 0.9286726664488185 2201 82-83 0.8851636889993983 2201 84-85 0.8614342832451669 2201 86-87 0.8376023947134712 2201 88-89 0.8684898377624677 2201 90-91 0.8944438845650424 2201 92-93 0.9023159198945976 2201 94-95 0.866901805787684 2201 96-97 0.8526043461583939 2201 98-99 0.8096479919673882 2201 100-101 0.6777419470821116 1101 1 0.40256760423833526 1101 2 0.7887961902581715 1101 3 0.7189375059246039 1101 4 0.7621749908680542 1101 5 0.5988743853949785 1101 6 0.8996125597885083 1101 7 0.645031503670026 1101 8 0.8638199997846598 1101 9 1.013553863936309 1101 10-11 0.8093646292578001 1101 12-13 0.621281079325648 1101 14-15 0.9898266424350552 1101 16-17 0.833306957563714 1101 18-19 0.8289025220101749 1101 20-21 0.9258708315624418 1101 22-23 1.012913377048676 1101 24-25 0.9547381734813811 1101 26-27 0.861790962864589 1101 28-29 0.9453075767566261 1101 30-31 0.907434818535684 1101 32-33 0.9111915320983996 1101 34-35 0.99871643938652 1101 36-37 0.9120831738814701 1101 38-39 0.8781341798149924 1101 40-41 0.8257811109308122 1101 42-43 0.8966298277459757 1101 44-45 0.8937228142854394 1101 46-47 0.841890378883221 1101 48-49 0.8789643339746007 1101 50-51 0.7573318328626044 1101 52-53 0.828083737255966 1101 54-55 0.8944234796903245 1101 56-57 0.9354716528931988 1101 58-59 0.942980026699864 1101 60-61 0.9974470760670187 1101 62-63 0.9870815643821302 1101 64-65 1.0255093391512986 1101 66-67 0.932963273021862 1101 68-69 0.8445674546256186 1101 70-71 0.8595244548443901 1101 72-73 0.9076150719247096 1101 74-75 0.925421510785533 1101 76-77 0.939576314110596 1101 78-79 0.9070387605877173 1101 80-81 0.8851418717108288 1101 82-83 0.8565058285949618 1101 84-85 0.8602690842888663 1101 86-87 0.8530682568574122 1101 88-89 0.8774886690301358 1101 90-91 0.881526674301945 1101 92-93 0.8611704263090374 1101 94-95 0.8743549205952448 1101 96-97 0.8830592004745483 1101 98-99 0.9005824342374424 1101 100-101 0.8417562708481476 2304 1 0.37694211561824886 2304 2 0.7054851467852608 2304 3 0.6694033969480522 2304 4 0.6576742508007669 2304 5 0.4952692475272542 2304 6 0.81155564268483 2304 7 0.6546500176500558 2304 8 0.8250569442949853 2304 9 0.9862081031822783 2304 10-11 0.7184838448743136 2304 12-13 0.5347632606135804 2304 14-15 0.833134441250948 2304 16-17 0.7245183565763753 2304 18-19 0.6961933047685847 2304 20-21 0.8058311490227581 2304 22-23 0.9279291391642559 2304 24-25 0.766994522248531 2304 26-27 0.7445590066713024 2304 28-29 0.8267581031486984 2304 30-31 0.8000448513885274 2304 32-33 0.7546095449408767 2304 34-35 0.7775983613214947 2304 36-37 0.7560202220451373 2304 38-39 0.7580901326351466 2304 40-41 0.8150440686964657 2304 42-43 0.7025194018010197 2304 44-45 0.7770320734714957 2304 46-47 0.7360206241375664 2304 48-49 0.7445456052213473 2304 50-51 0.6948896163061846 2304 52-53 0.7139894083235134 2304 54-55 0.768593319642541 2304 56-57 0.7828948407165655 2304 58-59 0.7974249872149599 2304 60-61 0.7774737689983837 2304 62-63 0.7420664226493656 2304 64-65 0.7349358913161623 2304 66-67 0.7588404833415225 2304 68-69 0.7293314291602755 2304 70-71 0.7332463015428559 2304 72-73 0.8149441262636614 2304 74-75 0.8089468333846028 2304 76-77 0.8443460697346694 2304 78-79 0.7927205173800829 2304 80-81 0.7939659937318879 2304 82-83 0.7715883779400983 2304 84-85 0.7926641023403604 2304 86-87 0.812789600661489 2304 88-89 0.8335909575290046 2304 90-91 0.8093869684841835 2304 92-93 0.8037754357081113 2304 94-95 0.787770753862695 2304 96-97 0.8123065443576891 2304 98-99 0.7627945326905419 2304 100-101 0.6422222442232943 2202 1 0.19656692895229355 2202 2 0.6118177538868146 2202 3 0.5874706177034135 2202 4 0.5859394829618978 2202 5 0.41486508313997916 2202 6 0.6885895393630008 2202 7 0.546509128180638 2202 8 0.7128337786550318 2202 9 0.88932964620588 2202 10-11 0.6834131018973295 2202 12-13 0.47525252003705276 2202 14-15 0.760233502279057 2202 16-17 0.7058045864750682 2202 18-19 0.5990293847374204 2202 20-21 0.7571887016040151 2202 22-23 0.848404192399677 2202 24-25 0.6678540495322522 2202 26-27 0.6418766824615432 2202 28-29 0.7427413949301638 2202 30-31 0.7356418582030457 2202 32-33 0.6635509360256862 2202 34-35 0.7936881949784933 2202 36-37 0.6707546813627374 2202 38-39 0.6739418864221705 2202 40-41 0.6448573158232236 2202 42-43 0.6705724360193699 2202 44-45 0.6354135143066841 2202 46-47 0.6713427522481226 2202 48-49 0.6509685677905068 2202 50-51 0.4879475761325125 2202 52-53 0.54930494275537 2202 54-55 0.6942694158340643 2202 56-57 0.7461795840899015 2202 58-59 0.755358057899592 2202 60-61 0.7086350863252946 2202 62-63 0.660803263067983 2202 64-65 0.7010539876990549 2202 66-67 0.7258588844212994 2202 68-69 0.7405818442884282 2202 70-71 0.6897154697462113 2202 72-73 0.6998392479239826 2202 74-75 0.7308198916165125 2202 76-77 0.7646334858001751 2202 78-79 0.7450382256701147 2202 80-81 0.7159687367143164 2202 82-83 0.7428071579221083 2202 84-85 0.7352512157647837 2202 86-87 0.7352093688790262 2202 88-89 0.7537539009036394 2202 90-91 0.758247022415695 2202 92-93 0.7371346247672577 2202 94-95 0.7136350011743247 2202 96-97 0.675523140958326 2202 98-99 0.7383127902421691 2202 100-101 0.6524878370565297 1102 1 0.2432337764975614 1102 2 0.5627126706712211 1102 3 0.5266712078049629 1102 4 0.5262395367186201 1102 5 0.2765065326454774 1102 6 0.6482314056843705 1102 7 0.4807994842600465 1102 8 0.6759793908033984 1102 9 0.7947168630774399 1102 10-11 0.6146065160873775 1102 12-13 0.3721709508415856 1102 14-15 0.6632239563608877 1102 16-17 0.5300131536213328 1102 18-19 0.45730180521220376 1102 20-21 0.56885157627989 1102 22-23 0.6883472752688178 1102 24-25 0.5104104933475284 1102 26-27 0.4397439567213155 1102 28-29 0.5560752092813317 1102 30-31 0.5430670691229054 1102 32-33 0.4942469082069465 1102 34-35 0.6143582548085647 1102 36-37 0.5106543116249966 1102 38-39 0.501458817703309 1102 40-41 0.5220249834343278 1102 42-43 0.4965545218816487 1102 44-45 0.49532540754218957 1102 46-47 0.3766676882424349 1102 48-49 0.49531310098804227 1102 50-51 0.36150031720369213 1102 52-53 0.41963725949131003 1102 54-55 0.5155260918199502 1102 56-57 0.5098482524466874 1102 58-59 0.5048112926799249 1102 60-61 0.59249361162599 1102 62-63 0.6068157693328899 1102 64-65 0.5032894296297172 1102 66-67 0.5574977936755943 1102 68-69 0.507397412808281 1102 70-71 0.5266645058073394 1102 72-73 0.5556376664685345 1102 74-75 0.46765335817389797 1102 76-77 0.5598311319533167 1102 78-79 0.5200836684385877 1102 80-81 0.5543640880546121 1102 82-83 0.5273946667436675 1102 84-85 0.5367634911113228 1102 86-87 0.5140597263180098 1102 88-89 0.5680779033854506 1102 90-91 0.5763753850061448 1102 92-93 0.5156745229604311 1102 94-95 0.5392017857798024 1102 96-97 0.49117042946378575 1102 98-99 0.5228926783101215 1102 100-101 0.5563206616936291 2305 1 0.30954129448515033 2305 2 0.5874898777524358 2305 3 0.580531459498328 2305 4 0.536153681573623 2305 5 0.3609867351518403 2305 6 0.6796829790930303 2305 7 0.4189459682998091 2305 8 0.660184345137445 2305 9 0.8401612361722925 2305 10-11 0.6038087869786892 2305 12-13 0.39346430714471836 2305 14-15 0.5880750482521009 2305 16-17 0.5834483122751593 2305 18-19 0.5159839056233508 2305 20-21 0.601316973410313 2305 22-23 0.6500133976927742 2305 24-25 0.548203170270078 2305 26-27 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1213 86-87 -1.1401552129995238 1213 88-89 -1.2884469585780138 1213 90-91 -1.291051631296945 1213 92-93 -1.3429073740828628 1213 94-95 -1.1251014279186293 1213 96-97 -1.2882268579691996 1213 98-99 -1.1484878642214724 1213 100-101 -1.038947312728343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11575.0 3 971.0 4 580.0 5 476.0 6 447.0 7 865.0 8 1981.0 9 8229.0 10 30406.0 11 40717.0 12 30432.0 13 27977.0 14 30060.0 15 32917.0 16 37565.0 17 42030.0 18 47332.0 19 52614.0 20 57959.0 21 63785.0 22 70092.0 23 78194.0 24 87259.0 25 99480.0 26 115223.0 27 138748.0 28 153891.0 29 176850.0 30 214999.0 31 268836.0 32 337414.0 33 457117.0 34 645079.0 35 977599.0 36 1952024.0 37 4554231.0 38 3435760.0 39 2879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.048946692947922 12.869580191679672 14.6021687647173 50.479304350655106 2 24.00896213195725 18.38355810630205 35.59726260044826 22.010217161292438 3 25.770150888120842 23.0832279497184 24.629400272659733 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25.567856950298157 48-49 24.949659828934394 24.716584880724252 24.50739554828637 25.826359742054983 50-51 24.911346579375348 24.54695436622609 25.087391674734246 25.454307379664314 52-53 25.11173037654623 24.756208468444118 24.93117701709509 25.200884137914553 54-55 24.848400613730558 24.706761052866096 24.825711376434732 25.619126956968614 56-57 24.54381801787875 24.700746035262064 25.465351416378017 25.290084530481167 58-59 24.983504958498298 24.84705975512953 24.897668725327733 25.27176656104444 60-61 24.92840121638224 24.977375197807163 24.753828487917637 25.340395097892955 62-63 24.6699207487495 25.09338349099271 24.895384986193562 25.34131077406422 64-65 24.902662802127875 25.121427797444458 24.415770992549866 25.560138407877798 66-67 24.733970889833166 25.157409973730843 24.422984270390486 25.685634866045508 68-69 24.89658161791013 25.005319700790523 24.659357825832593 25.43874085546675 70-71 25.022052442628613 25.008146159693602 24.4021279380833 25.567673459594488 72-73 24.91090904694809 25.032832019690453 24.772316890400027 25.28394204296143 74-75 25.333728428119155 24.876924912733735 24.5779571603767 25.211389498770405 76-77 25.538066615604375 24.877482406053485 24.321256140780463 25.26319483756168 78-79 25.048793380044003 24.827618458870283 24.52633945076151 25.597248710324198 80-81 25.280673748773708 24.928209763752506 24.350684579692768 25.44043190778102 82-83 25.240509932424764 24.897700191112886 24.23232307446815 25.629466801994198 84-85 25.200124550910292 24.749903005593158 24.239112517997228 25.810859925499326 86-87 25.16622914041194 25.09593269482393 24.379971403457795 25.357866761306337 88-89 25.365674099821995 25.02558271546106 24.33449899785555 25.274244186861395 90-91 25.45763172293719 24.77324554268878 24.34185576625818 25.427266968115852 92-93 25.37567139989225 24.950971199810738 24.65042146618432 25.022935934112695 94-95 25.221021837308268 24.854891376881856 24.432621823531026 25.491464962278855 96-97 25.05267337225659 25.021157816693407 24.387931647927903 25.538237163122098 98-99 25.29281904733942 24.680032862905424 24.59323675688433 25.433911332870828 100-101 25.41938463440061 24.811917356870595 24.380372025534168 25.388325983194626 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10305.0 1 5469.5 2 517.0 3 342.0 4 294.5 5 297.0 6 294.5 7 281.0 8 283.0 9 281.0 10 286.5 11 342.5 12 374.5 13 420.5 14 487.5 15 551.0 16 650.0 17 814.0 18 1043.5 19 1295.5 20 1666.5 21 2311.0 22 3184.5 23 4419.5 24 6191.0 25 8612.0 26 12050.5 27 16527.0 28 22561.0 29 30326.5 30 39925.0 31 52284.5 32 67035.0 33 85460.5 34 110341.5 35 143983.5 36 184038.5 37 236168.0 38 291446.0 39 341442.5 40 394321.0 41 443714.0 42 486054.0 43 524961.5 44 562118.5 45 572753.0 46 561332.0 47 567693.5 48 553061.0 49 523535.5 50 526631.0 51 506603.0 52 467259.0 53 439196.5 54 419896.5 55 418150.0 56 428958.5 57 434719.5 58 408356.0 59 358537.0 60 328538.5 61 304872.5 62 272238.0 63 248462.5 64 219290.0 65 197694.0 66 195946.5 67 196973.0 68 183158.5 69 158277.5 70 135810.5 71 119636.0 72 96832.0 73 74999.5 74 70722.5 75 61437.5 76 41379.0 77 26029.5 78 19368.5 79 18407.0 80 15790.5 81 10787.0 82 5201.0 83 1589.0 84 934.5 85 644.0 86 484.5 87 360.5 88 301.5 89 259.0 90 195.0 91 177.5 92 159.0 93 108.0 94 81.0 95 55.5 96 29.0 97 20.0 98 18.0 99 10.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1572673439138238 2 0.131547325149551 3 0.12923014327394555 4 0.13926893121841133 5 0.14484136859902133 6 0.09763666350171825 7 0.08563072115530349 8 0.09487144645983263 9 0.10670937561889232 10-11 0.0790712062989824 12-13 0.0910456461727681 14-15 0.12540434298688105 16-17 0.1247252896879876 18-19 0.12454677567642285 20-21 0.14861816503977396 22-23 0.1262269075499736 24-25 0.11300987014470766 26-27 0.11603760779183558 28-29 0.10465821462326579 30-31 0.08351655521441878 32-33 0.10346462093809743 34-35 0.12083648445566492 36-37 0.11875032071267273 38-39 0.11210679926267414 40-41 0.15642377770231186 42-43 0.11941887318735647 44-45 0.1184702987337476 46-47 0.15262597961313984 48-49 0.16011306727465038 50-51 0.1305322454759474 52-53 0.09620505113446355 54-55 0.09457392310722469 56-57 0.10049988823622766 58-59 0.10077640994041621 60-61 0.09625755525551201 62-63 0.08584773818897044 64-65 0.08700632912677317 66-67 0.10506424649270722 68-69 0.10167248027297662 70-71 0.09846272833954736 72-73 0.11024465310282205 74-75 0.14945473070181278 76-77 0.16507295657636167 78-79 0.1533120334615064 80-81 0.12621290645102734 82-83 0.10512025088849225 84-85 0.1101116426628326 86-87 0.11015364595967138 88-89 0.10635934814523591 90-91 0.11485101465614035 92-93 0.10087091735830345 94-95 0.10276456599078462 96-97 0.09704511707123892 98-99 0.09654807805864682 100-101 0.09245625689160343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.4284593E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.053281943051985 #Duplication Level Percentage of deduplicated Percentage of total 1 77.4427835764357 39.537082643825286 2 13.37344174062783 13.655161834665053 3 3.879872797489588 5.942407195002413 4 1.732459418603324 3.5379095661140574 5 1.0082744508693844 2.5737859908105296 6 0.6575955423548788 2.014344637700269 7 0.38032825923315117 1.3591904094678162 8 0.2482041784013259 1.0137310319493171 9 0.19732385214360934 0.9066627231819113 >10 0.9173065100441479 8.325142436122727 >50 0.07541601251430381 2.611637231788414 >100 0.06826228035687365 7.239321824950301 >500 0.011938991278320576 4.198874872441958 >1k 0.006524862458156664 5.929257092319654 >5k 2.1233245268624222E-4 0.6979490383200961 >10k+ 5.5194736656474434E-5 0.4575414713401452 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 27865 0.19507031106871578 Illumina Single End PCR Primer 1 (100% over 50bp) CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA 14868 0.10408416956646928 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.21871116663946955 3.500274736564073E-5 0.0 2 0.21888618037629773 3.500274736564073E-5 0.0 3 0.2190541935636528 4.900384631189702E-5 0.0 4 0.21978225070885815 6.300494525815331E-5 1.4001098946256291E-5 5 0.21992226169832071 6.300494525815331E-5 1.4001098946256291E-5 6 0.22023728642461146 8.400659367753775E-5 1.4001098946256291E-5 7 0.22032829356776212 9.800769262379404E-5 1.4001098946256291E-5 8 0.22060831554668725 1.1200879157005033E-4 1.4001098946256291E-5 9 0.22093734137192428 1.1900934104317848E-4 1.4001098946256291E-5 10-11 0.22173190373712431 1.540120884088192E-4 1.4001098946256291E-5 12-13 0.2225999718717922 2.3451840734979286E-4 2.1001648419384437E-5 14-15 0.2236920575896002 2.6252060524230547E-4 3.1502472629076654E-5 16-17 0.22471763808741346 3.115244515542025E-4 3.500274736564073E-5 18-19 0.2256907144641783 3.220252757638947E-4 4.2003296838768874E-5 20-21 0.2268388045777713 3.395266494467151E-4 4.2003296838768874E-5 22-23 0.22775937683348765 3.6752884733922764E-4 4.550357157533295E-5 24-25 0.2286134438692093 3.885304957586121E-4 6.300494525815331E-5 26-27 0.2293730034870437 4.095321441779966E-4 8.050631894097368E-5 28-29 0.23017456640171685 4.2003296838768876E-4 9.10071431506659E-5 30-31 0.23081511667850807 4.270335178608169E-4 9.10071431506659E-5 32-33 0.23144166585635306 4.760373641727139E-4 1.1200879157005033E-4 34-35 0.2321102183310368 5.005392873286624E-4 1.1550906630661441E-4 36-37 0.23282427437729586 5.390423094308672E-4 1.1900934104317848E-4 38-39 0.23359433481933997 5.530434083771235E-4 1.1900934104317848E-4 40-41 0.2344379010308519 5.530434083771235E-4 1.2250961577974255E-4 42-43 0.2353759746602511 5.74045056796508E-4 1.2951016525287068E-4 44-45 0.23608653043177358 5.810456062696361E-4 1.3651071472599885E-4 46-47 0.2369510982917049 5.880461557427642E-4 1.4001098946256292E-4 48-49 0.23779466450321685 5.880461557427642E-4 1.4001098946256292E-4 50-51 0.23868723456104068 6.090478041621487E-4 1.6101263788194736E-4 52-53 0.23969181341043458 6.090478041621487E-4 1.6451291261851143E-4 54-55 0.2406158859408875 6.300494525815332E-4 1.7501373682820366E-4 56-57 0.24168346973553956 6.440505515277894E-4 1.820142863013318E-4 58-59 0.24322709089436428 6.510511010009176E-4 1.820142863013318E-4 60-61 0.24483021672371064 6.68552474683738E-4 1.820142863013318E-4 62-63 0.24638783898148164 6.965546725762505E-4 1.8901483577445994E-4 64-65 0.24803296810766678 7.105557715225068E-4 1.92515110511024E-4 66-67 0.24985661124541664 7.210565957321991E-4 2.0301593472071624E-4 68-69 0.25170125603158594 7.280571452053272E-4 2.1001648419384438E-4 70-71 0.25380142087352436 7.490587936247116E-4 2.1701703366697252E-4 72-73 0.2559995934080866 7.490587936247116E-4 2.3101813261322882E-4 74-75 0.25844978572368144 7.560593430978398E-4 2.3101813261322882E-4 76-77 0.26109949369926044 7.735607167806602E-4 2.4151895682292103E-4 78-79 0.2640572258516571 7.945623652000445E-4 2.450192315594851E-4 80-81 0.26771501295136657 8.190642883559932E-4 2.5552005576917733E-4 82-83 0.2723003728562655 8.295651125656853E-4 2.6252060524230547E-4 84-85 0.2776732945768914 8.330653873022493E-4 2.730214294519977E-4 86-87 0.2842538110816318 8.435662115119416E-4 2.730214294519977E-4 88-89 0.29149587951158284 8.785689588775823E-4 2.730214294519977E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGTATC 7050 0.0 27.832134 48-49 CGCGTGT 4335 0.0 27.506298 3 CATGCGT 5660 0.0 27.02547 5 ATCGGAA 12355 0.0 26.838621 3 ACATGCG 5810 0.0 26.819162 4 AGATCGG 11805 0.0 26.639858 1 TCGTGTA 7035 0.0 26.571495 14-15 GCCGTAT 6430 0.0 26.008617 48-49 GAGTGTA 6015 0.0 25.700834 28-29 TCGGAAG 12910 0.0 24.39691 4 GTAGGGA 7550 0.0 24.193485 18-19 CGCCGTA 8450 0.0 23.477856 46-47 TGTAGGG 6945 0.0 22.744083 18-19 AAGAGCG 13535 0.0 22.49108 8 GTCGTGT 7200 0.0 22.201786 14-15 CGTGTAG 7150 0.0 22.191332 16-17 GCGTGTA 5460 0.0 21.83879 4 AGAGTGT 8000 0.0 21.461685 26-27 GATCGGA 16090 0.0 21.404427 2 CGCGTAT 5610 0.0 21.169815 1 >>END_MODULE