FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 mx48-3.3420000000cdd1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 mx48-3.3420000000cdd1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14284593
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC278650.19507031106871578Illumina Single End PCR Primer 1 (100% over 50bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA148680.10408416956646928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC70500.027.83213448-49
CGCGTGT43350.027.5062983
CATGCGT56600.027.025475
ATCGGAA123550.026.8386213
ACATGCG58100.026.8191624
AGATCGG118050.026.6398581
TCGTGTA70350.026.57149514-15
GCCGTAT64300.026.00861748-49
GAGTGTA60150.025.70083428-29
TCGGAAG129100.024.396914
GTAGGGA75500.024.19348518-19
CGCCGTA84500.023.47785646-47
TGTAGGG69450.022.74408318-19
AAGAGCG135350.022.491088
GTCGTGT72000.022.20178614-15
CGTGTAG71500.022.19133216-17
GCGTGTA54600.021.838794
AGAGTGT80000.021.46168526-27
GATCGGA160900.021.4044272
CGCGTAT56100.021.1698151
Produced by FastQC (version 0.11.2)