FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 mx24-3.3420000000cc32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 mx24-3.3420000000cc32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19451787
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA71850.012.69467614-15
CTCGTAT52200.012.3765911
CGCGTAT16150.011.471621
GTGCACA94050.011.2086286
CTCGGAT106950.011.0154831
CCCGAAT49000.010.8581191
CCGGGAT92450.010.7905861
CTCGACT96050.010.7818131
CTCGAAT88650.010.7172631
TGCACAC102450.010.3359687
TTAGGTG88950.010.17444618-19
CTCGGTT81450.010.0315961
GTTTGGT145950.09.9923341
CTCGGGT110100.09.9668361
GAGTGTC95150.09.88299224-25
CTGGAAT170100.09.7186871
CCGCGTT61600.09.6396661
CTCAGAT148850.09.6061711
CTCGATT110050.09.582871
CCGATTA45700.09.5635933
Produced by FastQC (version 0.11.2)