##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 mx24-3.3420000000cc32.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 19451787 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06968228677396 33.0 33.0 33.0 33.0 33.0 2 31.56189613838564 33.0 33.0 33.0 27.0 33.0 3 31.63184297668898 33.0 33.0 33.0 27.0 33.0 4 35.51475851550297 37.0 37.0 37.0 33.0 37.0 5 35.691742974565784 37.0 37.0 37.0 33.0 37.0 6 35.282257306231045 37.0 37.0 37.0 33.0 37.0 7 35.62583761584475 37.0 37.0 37.0 33.0 37.0 8 35.381513636767664 37.0 37.0 37.0 33.0 37.0 9 35.18205653804455 37.0 37.0 37.0 33.0 37.0 10-11 35.43918461064786 37.0 37.0 37.0 33.0 37.0 12-13 35.726017409094595 37.0 37.0 37.0 33.0 37.0 14-15 37.70146935600313 40.0 37.0 40.0 33.0 40.0 16-17 37.769868136022666 40.0 37.0 40.0 33.0 40.0 18-19 37.83675769737762 40.0 37.0 40.0 33.0 40.0 20-21 37.63640235213351 40.0 37.0 40.0 33.0 40.0 22-23 37.53446421143723 40.0 37.0 40.0 33.0 40.0 24-25 37.67520104965163 40.0 37.0 40.0 33.0 40.0 26-27 37.67390255712753 40.0 37.0 40.0 33.0 40.0 28-29 37.52150478513876 40.0 37.0 40.0 33.0 40.0 30-31 37.50380029351545 40.0 37.0 40.0 33.0 40.0 32-33 37.52525446633772 40.0 37.0 40.0 33.0 40.0 34-35 37.306233869412615 40.0 37.0 40.0 33.0 40.0 36-37 37.35189237369297 40.0 37.0 40.0 33.0 40.0 38-39 37.297585872187476 40.0 37.0 40.0 33.0 40.0 40-41 37.20883896168512 40.0 37.0 40.0 33.0 40.0 42-43 37.12864134282367 40.0 37.0 40.0 33.0 40.0 44-45 36.99863796575605 40.0 37.0 40.0 33.0 40.0 46-47 36.871426440151744 40.0 37.0 40.0 33.0 40.0 48-49 36.71204072407332 40.0 37.0 40.0 33.0 40.0 50-51 35.2997408927005 37.0 35.0 38.5 30.0 38.5 52-53 35.635295641475 37.0 35.0 38.5 30.0 40.0 54-55 36.29882462726946 37.0 37.0 40.0 33.0 40.0 56-57 36.234406638320685 37.0 37.0 40.0 33.0 40.0 58-59 36.093954324093716 37.0 37.0 40.0 33.0 40.0 60-61 35.8935747394314 37.0 37.0 40.0 30.0 40.0 62-63 35.70499533024909 37.0 37.0 40.0 27.0 40.0 64-65 35.55159507967057 37.0 37.0 40.0 27.0 40.0 66-67 35.34621819064747 37.0 33.0 38.5 27.0 40.0 68-69 35.17450766862705 37.0 33.0 37.0 27.0 40.0 70-71 34.96260824262573 37.0 33.0 37.0 27.0 40.0 72-73 34.74491320514665 37.0 33.0 37.0 27.0 40.0 74-75 34.52878768927503 37.0 33.0 37.0 27.0 37.0 76-77 34.333744786532975 37.0 33.0 37.0 27.0 37.0 78-79 34.178891378977156 37.0 33.0 37.0 27.0 37.0 80-81 34.049812878374624 37.0 33.0 37.0 27.0 37.0 82-83 33.949058690597425 37.0 33.0 37.0 27.0 37.0 84-85 33.842767787864425 37.0 33.0 37.0 27.0 37.0 86-87 33.7411397729165 37.0 33.0 37.0 27.0 37.0 88-89 33.65024619588935 37.0 33.0 37.0 27.0 37.0 90-91 33.552503839364476 37.0 33.0 37.0 27.0 37.0 92-93 33.467971091807655 37.0 33.0 37.0 27.0 37.0 94-95 33.38271712516696 37.0 33.0 37.0 27.0 37.0 96-97 33.300382581816265 37.0 33.0 37.0 27.0 37.0 98-99 33.23581530067135 37.0 33.0 37.0 27.0 37.0 100-101 31.459536391180926 35.0 30.0 35.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.46575147078383594 2306 2 0.8166290097225826 2306 3 0.7703559569132032 2306 4 0.8024483226907364 2306 5 0.6560220265440506 2306 6 0.9720907713886646 2306 7 0.6971425999305367 2306 8 0.8811746095560977 2306 9 1.0485026380002864 2306 10-11 0.8281702940037547 2306 12-13 0.6315281445023402 2306 14-15 0.9740016511730119 2306 16-17 0.9689622052280669 2306 18-19 0.861200824737189 2306 20-21 0.9088676026676907 2306 22-23 1.0296823143764726 2306 24-25 0.9558323888932279 2306 26-27 0.8668160296797964 2306 28-29 0.9827783802563914 2306 30-31 0.9410989280399065 2306 32-33 0.9205546214575477 2306 34-35 1.099153220885718 2306 36-37 0.9367282021621577 2306 38-39 0.9269770191630258 2306 40-41 0.8899268094359698 2306 42-43 0.8898347563420188 2306 44-45 0.9245373497118266 2306 46-47 0.8985009122511869 2306 48-49 0.9230678319324213 2306 50-51 0.8797286255097063 2306 52-53 0.8813962834210614 2306 54-55 0.9782399095679182 2306 56-57 0.9423722375449799 2306 58-59 0.9346004386572488 2306 60-61 0.963185673951152 2306 62-63 0.9201397774544873 2306 64-65 0.9304833624375561 2306 66-67 0.9742763623986619 2306 68-69 0.9331221666425549 2306 70-71 0.8596489251988331 2306 72-73 0.9262833867732496 2306 74-75 0.8455056719647445 2306 76-77 0.8954219094843978 2306 78-79 0.861571511953791 2306 80-81 0.8745980915822784 2306 82-83 0.8544362670205103 2306 84-85 0.9110631716864077 2306 86-87 0.8680335997837716 2306 88-89 0.8617116562573415 2306 90-91 0.9030878637167845 2306 92-93 0.9204244014228777 2306 94-95 0.9190162243978861 2306 96-97 0.9530101332084584 2306 98-99 0.8265166576723431 2306 100-101 0.7695548559668026 2307 1 0.33924603103797324 2307 2 0.7804179558292361 2307 3 0.7317782702704427 2307 4 0.7559127273185808 2307 5 0.5838096574965448 2307 6 0.8851022600234089 2307 7 0.6564538512566642 2307 8 0.8690441991293483 2307 9 0.962771248888636 2307 10-11 0.787734206226375 2307 12-13 0.5189069211722881 2307 14-15 0.907985932065607 2307 16-17 0.8371793419714209 2307 18-19 0.7499224521918251 2307 20-21 0.8848398235035617 2307 22-23 0.9714360145759997 2307 24-25 0.8202021957677132 2307 26-27 0.7092660785812583 2307 28-29 0.9046464463870905 2307 30-31 0.8355787577460347 2307 32-33 0.8293385228776771 2307 34-35 0.8798864270058999 2307 36-37 0.8225638195685221 2307 38-39 0.7977885469618329 2307 40-41 0.7571722088918662 2307 42-43 0.7722427194086023 2307 44-45 0.8031638423849188 2307 46-47 0.6789968932418304 2307 48-49 0.7657769098221294 2307 50-51 0.6567368879533788 2307 52-53 0.7638140941778744 2307 54-55 0.7751882486946329 2307 56-57 0.8051553536118163 2307 58-59 0.7846489921576918 2307 60-61 0.8371052784318422 2307 62-63 0.8105096529215459 2307 64-65 0.7748833880707977 2307 66-67 0.7173110441720709 2307 68-69 0.7017539569586191 2307 70-71 0.7379595757549282 2307 72-73 0.722837138824282 2307 74-75 0.6687652478044868 2307 76-77 0.7139158857631926 2307 78-79 0.7842428199294957 2307 80-81 0.7055886222359149 2307 82-83 0.7541365974190484 2307 84-85 0.7771491457759936 2307 86-87 0.780208479600816 2307 88-89 0.6899815461510244 2307 90-91 0.719862546626338 2307 92-93 0.6836198432049372 2307 94-95 0.6777687216801098 2307 96-97 0.7167348700591063 2307 98-99 0.7648946628952018 2307 100-101 0.6523878114620629 2201 1 0.3408416495536102 2201 2 0.7583184006295411 2201 3 0.7504388043334949 2201 4 0.7541525441870647 2201 5 0.6172675071124161 2201 6 0.8782171721531853 2201 7 0.6805757884379133 2201 8 0.8715023426353099 2201 9 1.0353375782329834 2201 10-11 0.8305781270139363 2201 12-13 0.6050520571334275 2201 14-15 0.9069558105655418 2201 16-17 0.8702037953305819 2201 18-19 0.8546170020510644 2201 20-21 0.945162110305958 2201 22-23 1.0388644497339143 2201 24-25 0.8979576658355768 2201 26-27 0.8509963275765031 2201 28-29 0.9649959170326454 2201 30-31 0.9275140495109895 2201 32-33 0.8809865669382404 2201 34-35 0.9916107979204227 2201 36-37 0.9100369501414036 2201 38-39 0.9319870751371937 2201 40-41 0.8402310331051339 2201 42-43 0.8497474097372404 2201 44-45 0.836315013287539 2201 46-47 0.8541708922913571 2201 48-49 0.8920303113378694 2201 50-51 0.5139643759364887 2201 52-53 0.7045121330970474 2201 54-55 0.8770444075836821 2201 56-57 0.8859600219228341 2201 58-59 0.8925697113744562 2201 60-61 0.9414910716009004 2201 62-63 0.8419051285649672 2201 64-65 0.8683387910850016 2201 66-67 0.8585642510274027 2201 68-69 0.8553864003716924 2201 70-71 0.8765469752944384 2201 72-73 0.8490412797352604 2201 74-75 0.9188285033746837 2201 76-77 0.8581214710829101 2201 78-79 0.899223076666253 2201 80-81 0.9207948719336656 2201 82-83 0.9062759823321827 2201 84-85 0.8380824749433273 2201 86-87 0.8236183910977957 2201 88-89 0.7999453629663265 2201 90-91 0.8261058447672198 2201 92-93 0.8204177559543808 2201 94-95 0.8169855111446225 2201 96-97 0.8550746500578512 2201 98-99 0.8089539128957028 2201 100-101 0.6939941979704685 1101 1 0.33075622273934613 1101 2 0.7840872010407764 1101 3 0.715504580122289 1101 4 0.748818837993241 1101 5 0.6004689823379223 1101 6 0.8708953450118031 1101 7 0.6228968187400383 1101 8 0.844155005069986 1101 9 1.0081192112866688 1101 10-11 0.8392060930611436 1101 12-13 0.6261469854475834 1101 14-15 0.9975255637730669 1101 16-17 0.8318709882889266 1101 18-19 0.8266578629393635 1101 20-21 0.9020845319731947 1101 22-23 1.016859694720452 1101 24-25 0.9549030703981245 1101 26-27 0.8610936802073041 1101 28-29 0.9300557714316326 1101 30-31 0.9233731912609642 1101 32-33 0.8845504275554319 1101 34-35 0.9874623919027243 1101 36-37 0.9100366580805854 1101 38-39 0.8924692742969356 1101 40-41 0.8566846626928921 1101 42-43 0.8905707367282929 1101 44-45 0.8837424050653127 1101 46-47 0.8203188765159695 1101 48-49 0.866298350675855 1101 50-51 0.776365958099781 1101 52-53 0.8363674997491373 1101 54-55 0.8845601029747172 1101 56-57 0.9106255482962737 1101 58-59 0.9071368714942878 1101 60-61 0.9541054014547399 1101 62-63 0.9563098215538801 1101 64-65 0.9383521274407229 1101 66-67 0.8894969557664538 1101 68-69 0.8498665311796643 1101 70-71 0.9038435658618269 1101 72-73 0.9498451187108614 1101 74-75 0.9471090410943717 1101 76-77 0.9444422863595605 1101 78-79 0.9582888140977275 1101 80-81 0.9592877683876182 1101 82-83 0.895572176876577 1101 84-85 0.9228818194580697 1101 86-87 0.9087738751405823 1101 88-89 0.9718957485687767 1101 90-91 0.9368074643480853 1101 92-93 0.9033596538286446 1101 94-95 0.9357941662259321 1101 96-97 0.9458386065259745 1101 98-99 0.9697873873392666 1101 100-101 0.9626503655243113 2304 1 0.3564805216853273 2304 2 0.6773487189274192 2304 3 0.6611182541231351 2304 4 0.6228050329246315 2304 5 0.4652264702480977 2304 6 0.7776249995615814 2304 7 0.5699490205401858 2304 8 0.7619701696853269 2304 9 0.9133830698757777 2304 10-11 0.6809055348813473 2304 12-13 0.47612042823908496 2304 14-15 0.7889399576718148 2304 16-17 0.6983925471055841 2304 18-19 0.641343367903751 2304 20-21 0.7423804693428977 2304 22-23 0.9072573687409431 2304 24-25 0.7250393587269812 2304 26-27 0.7011222250254292 2304 28-29 0.7740207207418521 2304 30-31 0.7341857629057245 2304 32-33 0.6852542568116391 2304 34-35 0.7290942181977087 2304 36-37 0.722641294552524 2304 38-39 0.707655786214751 2304 40-41 0.6976130713998359 2304 42-43 0.6514836362254854 2304 44-45 0.7114398207887902 2304 46-47 0.70240037299655 2304 48-49 0.6929282059881317 2304 50-51 0.6344430134770036 2304 52-53 0.6670270451132012 2304 54-55 0.6897325804548515 2304 56-57 0.74832039822995 2304 58-59 0.7465736069156392 2304 60-61 0.7426127457483105 2304 62-63 0.6605515396435848 2304 64-65 0.6859583389560271 2304 66-67 0.7259341982921086 2304 68-69 0.7315213782691359 2304 70-71 0.7068533072559191 2304 72-73 0.777043204737403 2304 74-75 0.7397017525881537 2304 76-77 0.7685871174727623 2304 78-79 0.762761824644933 2304 80-81 0.7385266199707274 2304 82-83 0.7340807971958228 2304 84-85 0.7801037094680794 2304 86-87 0.8134739012625758 2304 88-89 0.7735780810134187 2304 90-91 0.7351116225854071 2304 92-93 0.7481285138777025 2304 94-95 0.7410203887326858 2304 96-97 0.749875544947308 2304 98-99 0.6840229059879732 2304 100-101 0.6882858300726369 2202 1 0.22113907153490686 2202 2 0.6280973019619438 2202 3 0.6094142953178867 2202 4 0.5791958983379502 2202 5 0.4164098565652452 2202 6 0.7004554635668114 2202 7 0.5065281816262797 2202 8 0.6733271313887599 2202 9 0.8267423844399104 2202 10-11 0.6685954581473936 2202 12-13 0.4693461276945001 2202 14-15 0.7356553839234081 2202 16-17 0.6646901008211898 2202 18-19 0.5617839315672839 2202 20-21 0.717186264776835 2202 22-23 0.8387193090140244 2202 24-25 0.6707354667599361 2202 26-27 0.6326224265572051 2202 28-29 0.7398818305553121 2202 30-31 0.7182034154057817 2202 32-33 0.6502121413261222 2202 34-35 0.7707824554729967 2202 36-37 0.6659185595550667 2202 38-39 0.6533450598848489 2202 40-41 0.6571938542634754 2202 42-43 0.6550752130918838 2202 44-45 0.6265391548264958 2202 46-47 0.659368671307206 2202 48-49 0.6558067216351375 2202 50-51 0.5353277796646836 2202 52-53 0.5958199841906477 2202 54-55 0.7020342287620167 2202 56-57 0.701097719980055 2202 58-59 0.7278143535002428 2202 60-61 0.7090381020494405 2202 62-63 0.6859194245508959 2202 64-65 0.7508250348201315 2202 66-67 0.7550592602161785 2202 68-69 0.7473893014023147 2202 70-71 0.742484349286606 2202 72-73 0.7427459137592862 2202 74-75 0.7275513019892941 2202 76-77 0.7746780655312193 2202 78-79 0.7493787045237923 2202 80-81 0.7462222689803184 2202 82-83 0.7408624274549069 2202 84-85 0.7721411441375778 2202 86-87 0.7535066837831295 2202 88-89 0.7700646412127767 2202 90-91 0.7888169100280322 2202 92-93 0.7761954544172127 2202 94-95 0.7349842775906268 2202 96-97 0.7362027502631392 2202 98-99 0.7130048895013061 2202 100-101 0.630193997144822 1102 1 0.23423227723411344 1102 2 0.5655374937083408 1102 3 0.48203595269356114 1102 4 0.4920776031293883 1102 5 0.29310741678216345 1102 6 0.6079435851022907 1102 7 0.44165347448723935 1102 8 0.6147177289227628 1102 9 0.738273020232576 1102 10-11 0.5761463779005567 1102 12-13 0.34448973476987277 1102 14-15 0.6167161467110347 1102 16-17 0.49357900909565444 1102 18-19 0.41996292459725026 1102 20-21 0.5645677445514394 1102 22-23 0.6668604635504849 1102 24-25 0.5157903813370055 1102 26-27 0.39969826257342334 1102 28-29 0.5254354651599726 1102 30-31 0.5414842672662985 1102 32-33 0.5023423659323569 1102 34-35 0.618617366279814 1102 36-37 0.4685504935548437 1102 38-39 0.4727189063319983 1102 40-41 0.47813009577892274 1102 42-43 0.46868757428112673 1102 44-45 0.5396124823371693 1102 46-47 0.4145120196219665 1102 48-49 0.523374368439498 1102 50-51 0.36904366644510134 1102 52-53 0.4462950169484401 1102 54-55 0.5211192424243407 1102 56-57 0.523513032335849 1102 58-59 0.5115395113087899 1102 60-61 0.5438480621684647 1102 62-63 0.5997865673959666 1102 64-65 0.5364043559239562 1102 66-67 0.6275614644203742 1102 68-69 0.5673261214851308 1102 70-71 0.5511645290357805 1102 72-73 0.5770433291144315 1102 74-75 0.5616177633811361 1102 76-77 0.5778331983855622 1102 78-79 0.5796206786338587 1102 80-81 0.5819919309629569 1102 82-83 0.5734545128734325 1102 84-85 0.5979268943676672 1102 86-87 0.5535865333309573 1102 88-89 0.5448641739787234 1102 90-91 0.581167635478856 1102 92-93 0.5202328343192093 1102 94-95 0.5377406927418065 1102 96-97 0.521239526200219 1102 98-99 0.5381064516589618 1102 100-101 0.5886623419531603 2305 1 0.2719071651442775 2305 2 0.49877110602307084 2305 3 0.5157429454740985 2305 4 0.4194762821877376 2305 5 0.29915299238047766 2305 6 0.6045026157740239 2305 7 0.38072987833666616 2305 8 0.5831873617047947 2305 9 0.7450479237189072 2305 10-11 0.5233325706802816 2305 12-13 0.3203964297454931 2305 14-15 0.5036904447867272 2305 16-17 0.5175038426883702 2305 18-19 0.42647155422419303 2305 20-21 0.5170460258144018 2305 22-23 0.5937064154059115 2305 24-25 0.4458062768025073 2305 26-27 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1213 78-79 -1.2755594008553501 1213 80-81 -1.1382188393390038 1213 82-83 -1.1066867015471828 1213 84-85 -1.1853117099457648 1213 86-87 -1.1277034410149156 1213 88-89 -1.2353479553244426 1213 90-91 -1.2349748199895032 1213 92-93 -1.2847750233123634 1213 94-95 -1.096572832289283 1213 96-97 -1.2461855953590941 1213 98-99 -1.1491496658659912 1213 100-101 -1.0102432059497275 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 16126.0 3 1318.0 4 813.0 5 610.0 6 644.0 7 1378.0 8 2721.0 9 6153.0 10 15458.0 11 24082.0 12 26850.0 13 31657.0 14 36206.0 15 42068.0 16 48885.0 17 55837.0 18 62980.0 19 69801.0 20 77359.0 21 85347.0 22 94128.0 23 104054.0 24 116279.0 25 130882.0 26 149343.0 27 176093.0 28 205526.0 29 243875.0 30 295718.0 31 369714.0 32 463382.0 33 620971.0 34 861715.0 35 1284106.0 36 2544325.0 37 6127636.0 38 5054194.0 39 3553.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.208204230147675 12.512525851746334 10.874377955427766 56.40489196267823 2 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25.024958829158955 24.71600284231993 24.96005159020162 25.298986738319496 46-47 25.15935087003203 24.53741193710123 24.631949826593434 25.67128736627331 48-49 24.8500067673476 24.549547862139924 24.73672932404097 25.86371604647151 50-51 24.98812360958979 24.726247174137185 24.894270719105304 25.391358497167722 52-53 25.185851283620273 24.535161127718162 24.685433803772096 25.593553784889465 54-55 24.71552934193225 24.52521970605001 24.88958575376739 25.869665198250352 56-57 24.804197660051848 24.824566156934484 25.071828799232854 25.29940738378082 58-59 25.057738553817536 24.68919010403245 24.65860360095284 25.594467741197167 60-61 24.7073672317425 24.771948895268896 24.816003367097537 25.70468050589107 62-63 24.849514389635367 24.889882977404472 24.878807407310408 25.381795225649757 64-65 25.004397443022913 24.794238643424908 24.558226454950336 25.64313745860184 66-67 24.77861969481682 24.767418513075416 24.768295977576642 25.685665814531127 68-69 24.988156585799004 24.939429469461103 24.79196413507207 25.28044980966782 70-71 25.13581212334429 24.752901094336966 24.562901906637975 25.548384875680764 72-73 24.862892420266846 24.702151281744857 24.78855376104516 25.646402536943132 74-75 25.034240813907164 24.947624162611525 24.810323666855666 25.20781135662564 76-77 25.18557284648742 24.81195926000889 24.476428733324887 25.526039160178804 78-79 24.93398417679798 24.752233581155316 24.74949179832352 25.56429044372318 80-81 25.17617190387447 24.98277633886941 24.634761610322748 25.206290146933373 82-83 25.28043688491716 24.83335023929005 24.404062452508285 25.4821504232845 84-85 24.877600016160535 24.833493073035463 24.74981340112524 25.53909350967876 86-87 25.104049124022353 25.109774804484097 24.644615377093317 25.141560694400233 88-89 25.250132219389005 24.865600435309485 24.378972870019815 25.505294475281698 90-91 25.095992648549913 24.71397273737754 24.586362553638637 25.603672060433908 92-93 25.19170716453205 25.01002030925053 24.6222818793466 25.175990646870822 94-95 25.379011313498644 24.82381679870157 24.448647189884372 25.348524697915416 96-97 25.05201109793082 24.87282023538603 24.65568031792297 25.419488348760176 98-99 25.252526058206794 25.000064967820347 24.62098995777606 25.1264190161968 100-101 25.5635150591564 24.82806893323198 24.21653549030235 25.391880517309275 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 14353.0 1 7620.0 2 723.0 3 498.0 4 408.0 5 390.5 6 389.0 7 351.0 8 344.5 9 377.5 10 376.5 11 381.0 12 423.5 13 464.5 14 513.0 15 615.0 16 714.0 17 842.0 18 1073.0 19 1347.5 20 1729.0 21 2365.5 22 3279.5 23 4557.5 24 6636.5 25 9781.0 26 13993.5 27 19844.0 28 28013.0 29 38387.5 30 51493.5 31 68493.0 32 89673.0 33 116460.0 34 150855.0 35 193826.5 36 247083.0 37 313923.5 38 392055.5 39 475069.5 40 558921.0 41 637801.5 42 709053.5 43 760847.0 44 788225.0 45 793714.5 46 782165.0 47 758987.5 48 724012.5 49 687901.5 50 654119.5 51 619536.0 52 593104.0 53 579015.0 54 568100.5 55 561558.5 56 559532.5 57 551368.0 58 529963.0 59 498307.5 60 473881.0 61 454171.0 62 430828.5 63 397920.5 64 350688.0 65 315816.5 66 298931.5 67 280865.0 68 251577.0 69 214825.5 70 182483.0 71 151961.0 72 124388.5 73 100279.0 74 76126.0 75 56008.5 76 39895.5 77 27699.5 78 18841.0 79 12589.0 80 8118.0 81 5075.0 82 3133.0 83 1952.0 84 1278.0 85 899.5 86 641.5 87 466.5 88 366.0 89 310.0 90 260.0 91 199.5 92 162.5 93 130.0 94 86.5 95 59.5 96 42.5 97 25.0 98 13.0 99 9.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15912162723147236 2 0.13321655228900048 3 0.13163829112461495 4 0.14149856771514105 5 0.1474568891793849 6 0.0991836893957352 7 0.08728761013062708 8 0.09674175436940574 9 0.10785641442608847 10-11 0.08098484730477462 12-13 0.09282180603766636 14-15 0.12682896435170712 16-17 0.12649480482178835 18-19 0.12600641781652244 20-21 0.14989882420571435 22-23 0.12815789109761483 24-25 0.1146964029577334 26-27 0.1179994413880843 28-29 0.10622160318740895 30-31 0.0857530467509232 32-33 0.10500834704801158 34-35 0.12260313152719594 36-37 0.12015605558502157 38-39 0.11423886144753692 40-41 0.15758449339384603 42-43 0.12164435072212132 44-45 0.11973964140158434 46-47 0.15394215451773147 48-49 0.16105204113123386 50-51 0.1318156527212641 52-53 0.09825575408573001 54-55 0.0962353741586827 56-57 0.10188267021430987 58-59 0.10288771926198863 60-61 0.09781363532306826 62-63 0.08802019063852591 64-65 0.08841090024273862 66-67 0.10693876094777309 68-69 0.10334011985634019 70-71 0.09940217831914362 72-73 0.1120154153446159 74-75 0.1512688782783813 76-77 0.16652197559021184 78-79 0.15483153295889987 80-81 0.12864113718703582 82-83 0.10675625843527899 84-85 0.11176094001029313 86-87 0.11197942893370157 88-89 0.10802863510689276 90-91 0.11667308510009904 92-93 0.10282602827184978 94-95 0.1044068598941578 96-97 0.09847681346706089 98-99 0.09813751302129722 100-101 0.09430753071684365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.9451787E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.766347911833634 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76007988157019 38.70056305065837 2 12.78174939653129 13.233289723654165 3 4.947773151812254 7.683844390966282 4 2.3227441577910923 4.809599287295708 5 1.3364543597742613 3.4591680678180636 6 0.8424447396226741 2.616617249268087 7 0.6058648020895323 2.1954385692750664 8 0.3714689060384 1.538367090272964 9 0.3462436979726507 1.6131394558378875 >10 1.5460620451548568 14.410183734576115 >50 0.08887778308833875 3.1551320486181886 >100 0.046502785509744085 4.461366582923409 >500 0.0023243764317751486 0.8137014160413412 >1k 0.0013683350488564727 1.1461292587548078 >5k 4.15815642193262E-5 0.16346007403956261 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.042952351884173935 4.626824260413709E-5 5.140915844904121E-6 2 0.0436617982707707 4.626824260413709E-5 1.5422747534712363E-5 3 0.045841546589010046 4.626824260413709E-5 1.5422747534712363E-5 4 0.054031025529942316 4.626824260413709E-5 1.5422747534712363E-5 5 0.055537313872499224 6.683190598375358E-5 1.5422747534712363E-5 6 0.0601281517219986 8.225465351846593E-5 2.0563663379616484E-5 7 0.06125915320787751 9.253648520827418E-5 2.0563663379616484E-5 8 0.0636085517489987 9.76774010531783E-5 2.0563663379616484E-5 9 0.06693472430065166 1.1824106443279479E-4 2.0563663379616484E-5 10-11 0.07445845463966884 1.2595243820015095E-4 2.3134121302068544E-5 12-13 0.08235233091951911 1.3880472781241127E-4 3.0845495069424726E-5 14-15 0.09126924945250531 1.5936839119202776E-4 4.626824260413709E-5 16-17 0.10083649383987188 1.9278434418390457E-4 5.140915844904121E-5 18-19 0.11012612877161362 1.953548021063566E-4 5.140915844904121E-5 20-21 0.11711263340483834 2.082070917186169E-4 5.140915844904121E-5 22-23 0.12420452681288358 2.1848892340842516E-4 6.426144806130151E-5 24-25 0.13068722169330765 2.287707550982334E-4 8.225465351846593E-5 26-27 0.1362239880582694 2.3648212886558958E-4 9.510694313072623E-5 28-29 0.1413340584081041 2.467639605553978E-4 1.0281831689808242E-4 30-31 0.145950600836828 2.801799135472746E-4 1.1310014858789067E-4 32-33 0.1502304132777107 3.4187090368612406E-4 1.3880472781241127E-4 34-35 0.1543996960279279 3.598641091432885E-4 1.5679793326957568E-4 36-37 0.15802918261443022 3.907096042127132E-4 1.69650222881836E-4 38-39 0.16175634660198573 3.907096042127132E-4 1.69650222881836E-4 40-41 0.16544495372070445 3.958505200576173E-4 1.825025124940963E-4 42-43 0.16921838595086405 4.2669601512704204E-4 2.3905258678804162E-4 44-45 0.17255741079212927 4.421187626617544E-4 2.4933441847784985E-4 46-47 0.17625372928461536 4.5497105227401474E-4 2.54475334322754E-4 48-49 0.1795747609204234 4.7810517357608324E-4 2.724685397799184E-4 50-51 0.18313227468509707 4.960983790332477E-4 2.8789128731463077E-4 52-53 0.18646615861051735 5.115211265679601E-4 2.9560266108198696E-4 54-55 0.18997997459050936 5.372257057924807E-4 3.0074357692689104E-4 56-57 0.19353234743933811 5.50077995404741E-4 3.0331403484934314E-4 58-59 0.19664517198342754 5.655007429394533E-4 3.187367823840555E-4 60-61 0.20032349727045645 5.783530325517137E-4 3.3672998784122E-4 62-63 0.20422545239673867 6.066280696986863E-4 3.39300445763672E-4 64-65 0.20834846690435177 6.349031068456589E-4 3.4187090368612406E-4 66-67 0.2125023269070343 6.503258543803713E-4 3.650050249881926E-4 68-69 0.21677699843207207 6.657486019150837E-4 3.650050249881926E-4 70-71 0.22100797217242818 6.965940969845083E-4 3.7528685667800085E-4 72-73 0.22587384901962992 7.145873024416729E-4 3.855686883678091E-4 74-75 0.2312204014983302 7.377214237437415E-4 3.958505200576173E-4 76-77 0.23742291646520702 7.917010401152346E-4 4.0356189382497354E-4 78-79 0.24414980484826407 7.994124138825908E-4 4.1898464135968586E-4 80-81 0.25246780668531893 8.251169931071114E-4 4.292664730494941E-4 82-83 0.26325344812792784 8.328283668744676E-4 4.626824260413709E-4 84-85 0.2767380703891113 8.45680656486728E-4 4.67823341886275E-4 86-87 0.29242814554775864 8.636738619438923E-4 4.755347156536312E-4 88-89 0.31122847479257304 9.176534783153856E-4 4.781051735760833E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 7185 0.0 12.694676 14-15 CTCGTAT 5220 0.0 12.376591 1 CGCGTAT 1615 0.0 11.47162 1 GTGCACA 9405 0.0 11.208628 6 CTCGGAT 10695 0.0 11.015483 1 CCCGAAT 4900 0.0 10.858119 1 CCGGGAT 9245 0.0 10.790586 1 CTCGACT 9605 0.0 10.781813 1 CTCGAAT 8865 0.0 10.717263 1 TGCACAC 10245 0.0 10.335968 7 TTAGGTG 8895 0.0 10.174446 18-19 CTCGGTT 8145 0.0 10.031596 1 GTTTGGT 14595 0.0 9.992334 1 CTCGGGT 11010 0.0 9.966836 1 GAGTGTC 9515 0.0 9.882992 24-25 CTGGAAT 17010 0.0 9.718687 1 CCGCGTT 6160 0.0 9.639666 1 CTCAGAT 14885 0.0 9.606171 1 CTCGATT 11005 0.0 9.58287 1 CCGATTA 4570 0.0 9.563593 3 >>END_MODULE