##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 mx0-2.3420000000ca91.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 23595947 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.244883326784894 33.0 33.0 33.0 33.0 33.0 2 31.773716096243138 33.0 33.0 33.0 27.0 33.0 3 31.84328940050594 33.0 33.0 33.0 27.0 33.0 4 35.76092495884993 37.0 37.0 37.0 33.0 37.0 5 35.93881245791915 37.0 37.0 37.0 33.0 37.0 6 35.50505940702443 37.0 37.0 37.0 33.0 37.0 7 35.85943683463944 37.0 37.0 37.0 33.0 37.0 8 35.612803842965064 37.0 37.0 37.0 33.0 37.0 9 35.43005271201872 37.0 37.0 37.0 33.0 37.0 10-11 35.6823109070384 37.0 37.0 37.0 33.0 37.0 12-13 35.966054975458285 37.0 37.0 37.0 33.0 37.0 14-15 38.00429669129194 40.0 37.0 40.0 33.0 40.0 16-17 38.078305248778534 40.0 37.0 40.0 33.0 40.0 18-19 38.14597496341215 40.0 37.0 40.0 35.0 40.0 20-21 37.942110905741565 40.0 37.0 40.0 33.0 40.0 22-23 37.82560897004897 40.0 37.0 40.0 33.0 40.0 24-25 37.978223124505234 40.0 37.0 40.0 33.0 40.0 26-27 37.99777044337318 40.0 37.0 40.0 33.0 40.0 28-29 37.84171338832046 40.0 37.0 40.0 33.0 40.0 30-31 37.83495981322555 40.0 37.0 40.0 33.0 40.0 32-33 37.84451639088696 40.0 37.0 40.0 33.0 40.0 34-35 37.61871040819002 40.0 37.0 40.0 33.0 40.0 36-37 37.6602021948939 40.0 37.0 40.0 33.0 40.0 38-39 37.599191950210766 40.0 37.0 40.0 33.0 40.0 40-41 37.50167450367641 40.0 37.0 40.0 33.0 40.0 42-43 37.39979151928083 40.0 37.0 40.0 33.0 40.0 44-45 37.247245999493046 40.0 37.0 40.0 33.0 40.0 46-47 37.122347409917474 40.0 37.0 40.0 33.0 40.0 48-49 36.95047210862103 40.0 37.0 40.0 33.0 40.0 50-51 35.499825563263045 37.0 35.0 38.5 30.0 38.5 52-53 35.83861978499952 37.0 35.0 38.5 33.0 40.0 54-55 36.48723823205739 37.0 37.0 40.0 33.0 40.0 56-57 36.399362589685424 37.0 37.0 40.0 33.0 40.0 58-59 36.24945080610666 37.0 37.0 40.0 33.0 40.0 60-61 36.03230559044738 37.0 37.0 40.0 33.0 40.0 62-63 35.831718917659884 37.0 37.0 40.0 33.0 40.0 64-65 35.67859003497507 37.0 37.0 38.5 33.0 40.0 66-67 35.49904583189647 37.0 35.0 37.0 33.0 40.0 68-69 35.33882715112048 37.0 33.0 37.0 33.0 40.0 70-71 35.148462488070514 37.0 33.0 37.0 33.0 40.0 72-73 34.95866010378816 37.0 33.0 37.0 27.0 38.5 74-75 34.76741022515434 37.0 33.0 37.0 27.0 37.0 76-77 34.5961295386873 37.0 33.0 37.0 27.0 37.0 78-79 34.46104780198057 37.0 33.0 37.0 27.0 37.0 80-81 34.348280872134524 37.0 33.0 37.0 27.0 37.0 82-83 34.258695910785015 37.0 33.0 37.0 27.0 37.0 84-85 34.159094716562976 37.0 33.0 37.0 27.0 37.0 86-87 34.06892437925886 37.0 33.0 37.0 27.0 37.0 88-89 33.98559788678963 37.0 33.0 37.0 27.0 37.0 90-91 33.8906390364413 37.0 33.0 37.0 27.0 37.0 92-93 33.819279599161675 37.0 33.0 37.0 27.0 37.0 94-95 33.74436533528406 37.0 33.0 37.0 27.0 37.0 96-97 33.667311381908085 37.0 33.0 37.0 27.0 37.0 98-99 33.613808528218854 37.0 33.0 37.0 27.0 37.0 100-101 31.81905411552247 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.3887237592328816 2306 2 0.74566203620304 2306 3 0.714279233991153 2306 4 0.713661001693886 2306 5 0.6090506599053569 2306 6 0.8911513900340182 2306 7 0.647567480424776 2306 8 0.8421544407529709 2306 9 0.9801261779728065 2306 10-11 0.7782704804201117 2306 12-13 0.5838145163465995 2306 14-15 0.912927281862487 2306 16-17 0.8918397034171051 2306 18-19 0.7828759873256601 2306 20-21 0.8605699224716901 2306 22-23 0.9610564140880555 2306 24-25 0.8790704526647914 2306 26-27 0.8072956681307133 2306 28-29 0.9124990502932562 2306 30-31 0.8617740764684925 2306 32-33 0.8111965910604582 2306 34-35 0.9441162395254565 2306 36-37 0.83343339907303 2306 38-39 0.8060468636094313 2306 40-41 0.7901687560993764 2306 42-43 0.768737796831779 2306 44-45 0.802304210095798 2306 46-47 0.7722466296013692 2306 48-49 0.8190333599473476 2306 50-51 0.746919763604275 2306 52-53 0.7411714017384341 2306 54-55 0.8196294618090363 2306 56-57 0.7339818109102936 2306 58-59 0.7655473857738215 2306 60-61 0.8054057564272696 2306 62-63 0.8130151371549701 2306 64-65 0.7881187702276264 2306 66-67 0.8079689482567289 2306 68-69 0.7515352206461117 2306 70-71 0.7261715065133814 2306 72-73 0.7913753111878421 2306 74-75 0.6893325722510539 2306 76-77 0.7260136348039836 2306 78-79 0.7389879144103091 2306 80-81 0.695308934715321 2306 82-83 0.7123395339585272 2306 84-85 0.7766806209337673 2306 86-87 0.6938312548083303 2306 88-89 0.7501868385239945 2306 90-91 0.7674393986261805 2306 92-93 0.7120851264675423 2306 94-95 0.7484767314296548 2306 96-97 0.7178622886073285 2306 98-99 0.6371008831453366 2306 100-101 0.5286328764310362 2307 1 0.27162114978037977 2307 2 0.6695659251502981 2307 3 0.6100238336685244 2307 4 0.580168802955285 2307 5 0.4736523039081959 2307 6 0.7715429168565819 2307 7 0.51838026481024 2307 8 0.7267597417102465 2307 9 0.8404163449561679 2307 10-11 0.6558524623878697 2307 12-13 0.4101065982014589 2307 14-15 0.7702382191405874 2307 16-17 0.6898836864115694 2307 18-19 0.5836835485819094 2307 20-21 0.6919856758693186 2307 22-23 0.8288203981387312 2307 24-25 0.620953839429248 2307 26-27 0.5318207013574181 2307 28-29 0.7292456947996158 2307 30-31 0.7041514576768861 2307 32-33 0.6820032834398759 2307 34-35 0.7034036718626098 2307 36-37 0.6459558123630913 2307 38-39 0.6023128960304902 2307 40-41 0.5766232526659465 2307 42-43 0.5900767315849507 2307 44-45 0.5936811947786538 2307 46-47 0.554938709597181 2307 48-49 0.6022266317878362 2307 50-51 0.5010080288853729 2307 52-53 0.6106958800394864 2307 54-55 0.6561431578721582 2307 56-57 0.6442717534722675 2307 58-59 0.6099624057273516 2307 60-61 0.6771398184541937 2307 62-63 0.6644588836984298 2307 64-65 0.6021096474505541 2307 66-67 0.5616645676324552 2307 68-69 0.5557263759701101 2307 70-71 0.5891300437710782 2307 72-73 0.5592673936920818 2307 74-75 0.5230669009733404 2307 76-77 0.5957912954440516 2307 78-79 0.6145328664356313 2307 80-81 0.5794356849215987 2307 82-83 0.6025416058390363 2307 84-85 0.603085741134727 2307 86-87 0.6019399965831482 2307 88-89 0.5360014914164495 2307 90-91 0.4943135189361385 2307 92-93 0.4885264620727412 2307 94-95 0.4773516599112497 2307 96-97 0.5751377601441163 2307 98-99 0.5606531666512353 2307 100-101 0.3907369213050025 2201 1 0.2766697292908944 2201 2 0.6879796777295901 2201 3 0.6498679146207103 2201 4 0.6491055880985641 2201 5 0.5124588604339806 2201 6 0.8139919117868217 2201 7 0.5790622910746066 2201 8 0.7718157003869166 2201 9 0.8974036637224785 2201 10-11 0.7028367406644236 2201 12-13 0.49749437338285674 2201 14-15 0.7775516218927407 2201 16-17 0.7451780673131907 2201 18-19 0.7373603206066761 2201 20-21 0.8324091580158353 2201 22-23 0.9372772300167256 2201 24-25 0.806030842394577 2201 26-27 0.7485367332791384 2201 28-29 0.8694708618557385 2201 30-31 0.8108446346812173 2201 32-33 0.7809582431960891 2201 34-35 0.8569861545420707 2201 36-37 0.7939757361117117 2201 38-39 0.7974563114912101 2201 40-41 0.7219842852026019 2201 42-43 0.7213275002480941 2201 44-45 0.7233621824937089 2201 46-47 0.7338098679896135 2201 48-49 0.7636762963090789 2201 50-51 0.41632782691487336 2201 52-53 0.5855529086952487 2201 54-55 0.7489259234196481 2201 56-57 0.7928652707381474 2201 58-59 0.7750068515383077 2201 60-61 0.7979777637663688 2201 62-63 0.6981413609118405 2201 64-65 0.7283875072190682 2201 66-67 0.7579497040787047 2201 68-69 0.7682265467841347 2201 70-71 0.8098829236956391 2201 72-73 0.7576532010958488 2201 74-75 0.7980031873396527 2201 76-77 0.7799754534675287 2201 78-79 0.7949676564710231 2201 80-81 0.8317681505196575 2201 82-83 0.789181215563147 2201 84-85 0.7311168361209965 2201 86-87 0.7283958445723258 2201 88-89 0.7773969054013392 2201 90-91 0.7822680550735512 2201 92-93 0.7530822089400147 2201 94-95 0.7218678462488697 2201 96-97 0.7418505465992453 2201 98-99 0.7217635945062284 2201 100-101 0.651817456704503 1101 1 0.2835168185211998 1101 2 0.707661878697234 1101 3 0.6441665694528744 1101 4 0.6606717676234055 1101 5 0.5099524359475964 1101 6 0.8177884220064229 1101 7 0.5719530942628523 1101 8 0.803154630014248 1101 9 0.9439955638169266 1101 10-11 0.7464701983397362 1101 12-13 0.5276915558696444 1101 14-15 0.8871764247992431 1101 16-17 0.7470655231924539 1101 18-19 0.7212132952668 1101 20-21 0.8468117418674908 1101 22-23 0.9392495639265235 1101 24-25 0.8427602169954511 1101 26-27 0.7820452486835805 1101 28-29 0.8634194956776682 1101 30-31 0.8360056201381525 1101 32-33 0.8021305946923434 1101 34-35 0.9159431321270262 1101 36-37 0.8373964708153352 1101 38-39 0.7854964351574836 1101 40-41 0.7626952350492218 1101 42-43 0.8056695058461898 1101 44-45 0.7949258401870622 1101 46-47 0.7506388662463479 1101 48-49 0.790265246222674 1101 50-51 0.6824321228970405 1101 52-53 0.7081083196074829 1101 54-55 0.8123122785617056 1101 56-57 0.838457005224285 1101 58-59 0.8380109086402925 1101 60-61 0.8817471537395534 1101 62-63 0.8539234589851361 1101 64-65 0.8575868053595812 1101 66-67 0.8180960487352422 1101 68-69 0.7657141570803319 1101 70-71 0.8229230624446444 1101 72-73 0.8669399586751467 1101 74-75 0.8707761816314417 1101 76-77 0.8673263079491065 1101 78-79 0.8973692220879528 1101 80-81 0.8621643080749948 1101 82-83 0.8177474729148386 1101 84-85 0.8810239755586693 1101 86-87 0.8178557917948552 1101 88-89 0.8517083036271842 1101 90-91 0.8347247819836383 1101 92-93 0.8664990688917911 1101 94-95 0.8339593193513934 1101 96-97 0.8289558807253243 1101 98-99 0.8123153386573705 1101 100-101 0.790762751211286 2304 1 0.2897213906616187 2304 2 0.6273852372139963 2304 3 0.583934058696844 2304 4 0.5420879754676449 2304 5 0.40324045115292506 2304 6 0.7195466406836459 2304 7 0.5424962598862848 2304 8 0.6929821638555325 2304 9 0.8536197018262897 2304 10-11 0.6347041814036558 2304 12-13 0.4280225603122645 2304 14-15 0.7058459324895736 2304 16-17 0.6105618351845834 2304 18-19 0.5317963873537721 2304 20-21 0.6723106416282647 2304 22-23 0.7804487222370753 2304 24-25 0.6364781589892274 2304 26-27 0.6305895766829579 2304 28-29 0.6880598967343374 2304 30-31 0.6561919504580942 2304 32-33 0.6244329813941576 2304 34-35 0.6941966373214399 2304 36-37 0.6685753932883287 2304 38-39 0.6391585600385099 2304 40-41 0.6598641260005706 2304 42-43 0.6239238486564815 2304 44-45 0.6617133432114599 2304 46-47 0.6124480241744266 2304 48-49 0.6138094061423374 2304 50-51 0.5783508270496256 2304 52-53 0.5800615614121867 2304 54-55 0.5822672566761895 2304 56-57 0.6562633732274747 2304 58-59 0.6408438772806377 2304 60-61 0.6618576838524746 2304 62-63 0.5917589866270774 2304 64-65 0.5963091173938366 2304 66-67 0.5862432280164285 2304 68-69 0.6052933020543989 2304 70-71 0.627511901124123 2304 72-73 0.6598600865764155 2304 74-75 0.6900934454953997 2304 76-77 0.7241390606080174 2304 78-79 0.6641752276338906 2304 80-81 0.6730835627805831 2304 82-83 0.6583383649878414 2304 84-85 0.6940931248819595 2304 86-87 0.6704006279930681 2304 88-89 0.6698144804148356 2304 90-91 0.6639462842958821 2304 92-93 0.679617749754378 2304 94-95 0.6297568727364364 2304 96-97 0.6556960403491701 2304 98-99 0.6236288210444911 2304 100-101 0.6315923308657112 2202 1 0.20034258679498862 2202 2 0.5970594579396682 2202 3 0.5456917512656325 2202 4 0.5305925501846289 2202 5 0.36920884853623903 2202 6 0.6384952744029277 2202 7 0.49236530954505753 2202 8 0.6567819767504446 2202 9 0.7993219661130908 2202 10-11 0.6155747240924541 2202 12-13 0.42728496172743036 2202 14-15 0.7049549721237227 2202 16-17 0.6232836707750664 2202 18-19 0.5391520124306126 2202 20-21 0.7018727965218474 2202 22-23 0.8076048808184098 2202 24-25 0.6325006430687665 2202 26-27 0.5806319062951886 2202 28-29 0.6858874395043912 2202 30-31 0.6710246259542956 2202 32-33 0.6035994521438752 2202 34-35 0.7253031163013048 2202 36-37 0.6187978290929621 2202 38-39 0.5968006457884059 2202 40-41 0.559998059629514 2202 42-43 0.5876121205153026 2202 44-45 0.5815761944166766 2202 46-47 0.6026977778236784 2202 48-49 0.5925452973405996 2202 50-51 0.4142378315650248 2202 52-53 0.48439083656227666 2202 54-55 0.6143299964023896 2202 56-57 0.6326342912074168 2202 58-59 0.6492597094323003 2202 60-61 0.6394613629496462 2202 62-63 0.6409767724789575 2202 64-65 0.6673665537257492 2202 66-67 0.6711954700608018 2202 68-69 0.6482308070343947 2202 70-71 0.6489914620284338 2202 72-73 0.6453324627365618 2202 74-75 0.6839630610000071 2202 76-77 0.7313637116329588 2202 78-79 0.6241676224692938 2202 80-81 0.6599295730074672 2202 82-83 0.6613823945573003 2202 84-85 0.6746457209617915 2202 86-87 0.6971474894704315 2202 88-89 0.7120164988828677 2202 90-91 0.6983243677839468 2202 92-93 0.6943508891053085 2202 94-95 0.6799563298692632 2202 96-97 0.635553962654825 2202 98-99 0.6609281955535664 2202 100-101 0.5861685841800188 1102 1 0.2371550485865228 1102 2 0.577804483496859 1102 3 0.5040848610612123 1102 4 0.514233964367186 1102 5 0.3170329606253972 1102 6 0.6264584841050578 1102 7 0.44656266769994346 1102 8 0.6455217216656095 1102 9 0.7454059951904952 1102 10-11 0.5867240188542198 1102 12-13 0.36734355327099877 1102 14-15 0.6344688177558453 1102 16-17 0.5690393868779893 1102 18-19 0.4556625744910434 1102 20-21 0.6113553940792471 1102 22-23 0.6776676344878965 1102 24-25 0.5529580569573938 1102 26-27 0.4196053614012669 1102 28-29 0.5485626633216825 1102 30-31 0.5568401352017389 1102 32-33 0.48940158372663944 1102 34-35 0.6173028917954255 1102 36-37 0.522607353220998 1102 38-39 0.4797435810424844 1102 40-41 0.478277042656579 1102 42-43 0.4729867939061876 1102 44-45 0.5062334425225998 1102 46-47 0.3836781451690996 1102 48-49 0.48945719722483716 1102 50-51 0.3550366952948494 1102 52-53 0.45046431209556914 1102 54-55 0.4937187142867572 1102 56-57 0.5100937611236631 1102 58-59 0.5210712535399296 1102 60-61 0.5247462554884095 1102 62-63 0.545554113643739 1102 64-65 0.5012978798185301 1102 66-67 0.5579605546139774 1102 68-69 0.5503218829400112 1102 70-71 0.5137989482364844 1102 72-73 0.5504241774973337 1102 74-75 0.5586456036420913 1102 76-77 0.5966250613929844 1102 78-79 0.5783877620704772 1102 80-81 0.5773300400169674 1102 82-83 0.5712674591567293 1102 84-85 0.5820211822493491 1102 86-87 0.5775559031233044 1102 88-89 0.5828736839752082 1102 90-91 0.5998731824404402 1102 92-93 0.5523183347494012 1102 94-95 0.5825790592525522 1102 96-97 0.5011299371717115 1102 98-99 0.4791101403454263 1102 100-101 0.5456211345469875 2305 1 0.26602816912360794 2305 2 0.5161522544596373 2305 3 0.4906189357598727 2305 4 0.43314669393384264 2305 5 0.29213108454005976 2305 6 0.6054611304768827 2305 7 0.3726718330677983 2305 8 0.6004029153151791 2305 9 0.7349191392022263 2305 10-11 0.5210166105892213 2305 12-13 0.33257980415742594 2305 14-15 0.5345289062624445 2305 16-17 0.48690147315116405 2305 18-19 0.43715562691473053 2305 20-21 0.5491151100653084 2305 22-23 0.5962427861553081 2305 24-25 0.4828405405286631 2305 26-27 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68-69 -1.2900173615517545 1213 70-71 -1.186379206490308 1213 72-73 -1.1308670028074062 1213 74-75 -1.3714487190919513 1213 76-77 -1.3236394506081623 1213 78-79 -1.2451385720046702 1213 80-81 -1.1184568381557867 1213 82-83 -1.028299902807916 1213 84-85 -1.1280939810608217 1213 86-87 -1.0604891817407278 1213 88-89 -1.183560840226228 1213 90-91 -1.1604491982632084 1213 92-93 -1.2324951026800406 1213 94-95 -1.0294272626944547 1213 96-97 -1.2157839576293838 1213 98-99 -1.0873632222626881 1213 100-101 -0.9640377888941991 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19210.0 3 1482.0 4 1007.0 5 706.0 6 779.0 7 1436.0 8 2779.0 9 5825.0 10 13036.0 11 19102.0 12 22055.0 13 25695.0 14 29650.0 15 34867.0 16 41870.0 17 49145.0 18 56675.0 19 64735.0 20 73538.0 21 83213.0 22 93371.0 23 106114.0 24 120116.0 25 137278.0 26 158761.0 27 188768.0 28 222892.0 29 268958.0 30 330974.0 31 417055.0 32 531056.0 33 724496.0 34 1030250.0 35 1573816.0 36 3241883.0 37 8136410.0 38 5759876.0 39 7068.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.90962888796278 11.301813787659052 10.680086113973248 56.10847121040492 2 23.100053563309565 17.462203323471382 38.20157559814838 21.236167515070676 3 24.938721800475104 22.530687264272387 24.187321496915626 28.343269438336886 4 28.547255961313056 27.245150148043447 17.616223419086744 26.591370471556758 5 29.788166326046817 28.650354687318362 21.2444035188486 20.317075467786225 6 21.566534815936066 31.616681872707137 23.07102287803831 23.74576043331849 7 19.922343006531378 14.56235960665644 41.26462956008227 24.250667826729916 8 21.289953359714243 19.539135538499572 28.200684731724607 30.970226370061578 9 22.275365104769087 19.240118626249885 30.962795254152407 27.52172101482862 10-11 25.81346139380578 27.818941732019898 21.144890669668097 25.222706204506224 12-13 23.74729566603473 22.10460890301578 27.828400074614752 26.319695356334737 14-15 24.672159456100466 23.89839080715345 25.784683548122793 25.64476618862329 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24.14614368888389 24.49325794534227 25.580860967893877 42-43 25.34429410223093 24.109014986389983 24.856691350326372 25.68999956105272 44-45 25.366421964590717 24.303233415145844 24.744161762618692 25.586182857644747 46-47 25.564163087186763 24.196106305152036 24.29851934977723 25.94121125788397 48-49 25.26952874564724 24.115597279359378 24.53597317265497 26.07890080233841 50-51 25.307025260424687 24.461232220682376 24.892493843241986 25.339248675650943 52-53 25.543455367320504 24.186086930036232 24.580972017063285 25.689485685579978 54-55 25.064034959546884 24.366493321260247 24.774902771194096 25.794568947998776 56-57 24.96367075189925 24.28787217925069 25.00360116077964 25.74485590807042 58-59 25.72829578876638 24.09024195067062 24.429350199699908 25.75211206086309 60-61 25.52515253945087 24.003940738581356 24.64571701326156 25.82518970870622 62-63 25.410929428774104 24.376421241998145 24.890801595491908 25.321847733735847 64-65 25.17849411592842 24.49732087567279 24.53541774662236 25.788767261776425 66-67 25.180513852262433 24.522882637682773 24.5493345064076 25.7472690036472 68-69 25.320747671512706 24.595442991663482 24.646641543026806 25.437167793797 70-71 25.516535304235383 24.309014394347848 24.31484951942092 25.859600781995844 72-73 25.3868979035187 24.591420883853974 24.45314945679446 25.56853175583287 74-75 25.515549882347234 24.374696116653347 24.599161538216833 25.51059246278259 76-77 25.657064905232367 24.32670319916813 24.42013821403387 25.596093681565634 78-79 25.379186793494462 24.346268727163164 24.518694803828144 25.755849675514224 80-81 25.345527924429845 24.53712224858474 24.339170332405565 25.778179494579845 82-83 25.588644568984904 24.354495547513693 24.188209290190105 25.868650593311298 84-85 25.316676477549375 24.62334133626153 24.40344236293263 25.656539823256463 86-87 25.44637861560002 24.98044807473862 24.272009383821025 25.301163925840335 88-89 25.61122944207252 24.40539699489561 24.12162117730362 25.86175238572825 90-91 25.56695166994133 24.346217626552196 24.19840601485384 25.888424688652634 92-93 25.52108655669874 24.790918744649794 24.42656027202989 25.26143442662157 94-95 25.70800895232508 24.643588776711077 24.08562858931984 25.562773681644007 96-97 25.28918467820398 24.727917453315932 24.22798713023773 25.754910738242366 98-99 25.577331205120675 24.80829297897474 24.36628365951417 25.248092156390417 100-101 25.998468827818467 24.59563454734383 23.875541187242135 25.53035543759557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 17144.0 1 9102.5 2 848.5 3 555.0 4 469.0 5 470.0 6 458.0 7 472.0 8 480.5 9 493.5 10 566.0 11 703.0 12 919.0 13 1263.5 14 1570.5 15 1865.0 16 2579.0 17 3610.5 18 3991.0 19 3625.0 20 4031.5 21 5696.0 22 8712.5 23 12370.0 24 15690.5 25 21390.5 26 29685.5 27 39437.5 28 53338.0 29 72539.0 30 93138.5 31 112794.5 32 137880.0 33 167697.0 34 201642.0 35 249966.5 36 301384.5 37 360054.5 38 428302.5 39 496015.5 40 569663.0 41 640711.5 42 703588.0 43 753082.0 44 785346.0 45 787623.0 46 769629.5 47 761779.0 48 744290.0 49 750886.5 50 797356.5 51 798864.0 52 786572.5 53 809476.0 54 843162.5 55 893434.0 56 912718.5 57 860685.0 58 742073.5 59 636572.5 60 596775.5 61 559020.5 62 517306.5 63 473247.5 64 415751.5 65 381290.0 66 363782.5 67 355180.0 68 341606.5 69 287404.0 70 239518.0 71 202446.5 72 164540.0 73 134443.5 74 105870.0 75 81197.0 76 57210.0 77 38661.0 78 26429.5 79 19425.5 80 12904.5 81 7695.5 82 4073.0 83 1962.0 84 1364.0 85 1030.0 86 780.0 87 602.5 88 513.0 89 447.5 90 360.0 91 283.0 92 241.0 93 199.0 94 142.0 95 101.5 96 80.0 97 62.0 98 42.0 99 30.0 100 25.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1585060349559185 2 0.13268804172174145 3 0.13086145684256706 4 0.14099455300522584 5 0.14616917049355976 6 0.0978049323470679 7 0.0856969207466011 8 0.09465608648807357 9 0.10641658077974153 10-11 0.07947339430792924 12-13 0.09119998447190952 14-15 0.12517403942295682 16-17 0.12454257504477358 18-19 0.12415267757636513 20-21 0.14837293879325972 22-23 0.12610640293436834 24-25 0.11334573687591348 26-27 0.11624453979321109 28-29 0.10464297110007918 30-31 0.08408647468143576 32-33 0.10350506381456104 34-35 0.12085126314277618 36-37 0.11866444690692007 38-39 0.11220571058241485 40-41 0.1561518170896044 42-43 0.119497217043249 44-45 0.11795246022547855 46-47 0.15239905395617306 48-49 0.15996603145446972 50-51 0.13046732135819766 52-53 0.09645724327148218 54-55 0.0945098749374204 56-57 0.10038376505931293 58-59 0.10098344431778898 60-61 0.09620720032978546 62-63 0.08644069254774983 64-65 0.08696408751892858 66-67 0.1052447693665357 68-69 0.1016742409194257 70-71 0.09826899509479319 72-73 0.11019477200893867 74-75 0.14961255846184093 76-77 0.16499655640013092 78-79 0.153778528151466 80-81 0.12659165576189843 82-83 0.1053019825820087 84-85 0.11032615050372845 86-87 0.11026258026431404 88-89 0.10617077585400576 90-91 0.11482904246224998 92-93 0.10099827737365234 94-95 0.1025324391515204 96-97 0.09688952089950024 98-99 0.09663312093386207 100-101 0.09260488676296824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.3595947E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.76721282581166 #Duplication Level Percentage of deduplicated Percentage of total 1 75.0150692111304 26.830799456174926 2 12.532358914144954 8.964950969833609 3 3.996065359867566 4.287843605767107 4 2.4096645410489668 3.447479379140407 5 1.1727492630579694 2.097298624155409 6 0.8780638414527759 1.884353777513471 7 0.5775908678097417 1.4461170846637397 8 0.5303429662714407 1.5175111796242302 9 0.2836373015841384 0.9130424157988926 >10 2.17410792380369 14.82653226696364 >50 0.22279918424091355 5.625966017271803 >100 0.17312339041221111 12.50587865016832 >500 0.02046260412074514 4.8971383925876495 >1k 0.013153400594104414 8.578933632773627 >5k 7.023481126212564E-4 1.6791533015629463 >10k+ 1.0888234770647207E-4 0.4970012460000987 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.029021933300663882 4.6618175570575744E-5 0.0 2 0.029216882034868108 5.0856191531537174E-5 0.0 3 0.03017467364204539 5.5094207492498604E-5 0.0 4 0.03415417062938817 5.9332223453460034E-5 0.0 5 0.03487463334275162 6.357023941442146E-5 0.0 6 0.03693430909977887 8.899833518019006E-5 8.476031921922862E-6 7 0.037260636328772904 8.899833518019006E-5 1.2714047882884294E-5 8 0.03800652713790212 8.899833518019006E-5 1.2714047882884294E-5 9 0.03986277812880322 9.323635114115149E-5 1.2714047882884294E-5 10-11 0.04200509519706923 1.1230742296547793E-4 1.2714047882884294E-5 12-13 0.04411350813764754 1.229024628678815E-4 1.2714047882884294E-5 14-15 0.04626218222985498 1.250214708483622E-4 1.2714047882884294E-5 16-17 0.04823709766766301 1.4197353469220793E-4 1.2714047882884294E-5 18-19 0.05014632385807613 1.4409254267268865E-4 1.6952063843845727E-5 20-21 0.05155546416509581 1.5256857459461154E-4 2.9666111726730017E-5 22-23 0.05280779788155991 1.737586543994187E-4 2.9666111726730017E-5 24-25 0.0539075630234294 1.801156783408608E-4 2.9666111726730017E-5 26-27 0.05508785046855716 1.8647270228230298E-4 3.178511970721073E-5 28-29 0.056119807355051274 2.0130575814566797E-4 3.390412768769145E-5 30-31 0.05695257749138019 2.0766278208711014E-4 3.602313566817216E-5 32-33 0.05776203853992383 2.2037682996999442E-4 4.02611516291336E-5 34-35 0.05850369133309208 2.2673385391143658E-4 4.6618175570575744E-5 36-37 0.05931527138961619 2.5216194967720513E-4 4.6618175570575744E-5 38-39 0.06003785311096012 2.5428095765768586E-4 4.6618175570575744E-5 40-41 0.06070322161683106 2.563999656381666E-4 5.721321547297932E-5 42-43 0.06136647111472152 2.669950055405702E-4 6.78082553753829E-5 44-45 0.062029720612611985 2.860660773648966E-4 7.204627133634432E-5 46-47 0.06279256348558504 3.0089913322826163E-4 7.416527931682505E-5 48-49 0.06368042782940647 3.157321890916266E-4 8.687932719970935E-5 50-51 0.06479290701915885 3.390412768769145E-4 9.323635114115149E-5 52-53 0.06599226553611093 3.4751730879883734E-4 9.747436710211292E-5 54-55 0.06726367032439937 3.750644125450867E-4 9.747436710211292E-5 56-57 0.06842912471366375 3.920164763889324E-4 9.747436710211292E-5 58-59 0.06988276418827352 4.004925083108552E-4 1.0171238306307435E-4 60-61 0.07155254247689233 4.0473052427181667E-4 1.0595039902403578E-4 62-63 0.07373300168880698 4.1108754821325884E-4 1.1018841498499721E-4 64-65 0.07644109388786133 4.153255641742203E-4 1.1018841498499721E-4 66-67 0.0794882273637926 4.216825881156624E-4 1.1018841498499721E-4 68-69 0.08297187648370291 4.301586200375852E-4 1.1442643094595864E-4 70-71 0.08762945602479952 4.7253877964719955E-4 1.1866444690692007E-4 72-73 0.09417083366054348 4.8101481156912244E-4 1.1866444690692007E-4 74-75 0.10294564570771413 5.085619153153717E-4 1.1866444690692007E-4 76-77 0.11338387901956212 5.297519951201789E-4 1.1866444690692007E-4 78-79 0.12684593671955613 5.361090190616211E-4 1.2714047882884293E-4 80-81 0.14583648624062429 5.657751307883511E-4 1.3137849478980437E-4 82-83 0.17254870084256418 5.678941387688318E-4 1.377355187312465E-4 84-85 0.20991316856238065 5.742511627102739E-4 1.5468758257509226E-4 86-87 0.2581227191262974 5.763701706907546E-4 1.652826224774958E-4 88-89 0.3168171211776327 5.996792584760425E-4 1.7375865439941867E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 1845 0.0 23.946165 1 ACATGCG 4440 0.0 18.938929 4 CTTACGG 4915 0.0 17.011343 1 TAAGTCG 2225 0.0 16.863865 9 AACCGAT 8935 0.0 15.419177 5 AAAGGGC 15020 0.0 15.370374 2 GTTAGTT 5675 0.0 15.31915 1 TTACGGT 5065 0.0 15.099568 2 GTCCGTA 4585 0.0 14.817053 4 GCGCTTA 8210 0.0 14.642192 38-39 TACCCTA 5460 0.0 14.614255 9 GCGTATT 2825 0.0 14.460993 2 CGTCAAT 10390 0.0 14.448441 1 CTCGGTT 9260 0.0 14.313395 1 CCGCGTT 6015 0.0 14.21629 1 CGTGCGA 6065 0.0 13.782941 9 TCCCCTA 7930 0.0 13.657768 2 CCTAGGC 8465 0.0 13.576753 8 TACCTAG 8435 0.0 13.454479 6 ACTCGCG 9010 0.0 13.420214 34-35 >>END_MODULE