FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 mx0-2.3420000000ca91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 mx0-2.3420000000ca91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595947
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT18450.023.9461651
ACATGCG44400.018.9389294
CTTACGG49150.017.0113431
TAAGTCG22250.016.8638659
AACCGAT89350.015.4191775
AAAGGGC150200.015.3703742
GTTAGTT56750.015.319151
TTACGGT50650.015.0995682
GTCCGTA45850.014.8170534
GCGCTTA82100.014.64219238-39
TACCCTA54600.014.6142559
GCGTATT28250.014.4609932
CGTCAAT103900.014.4484411
CTCGGTT92600.014.3133951
CCGCGTT60150.014.216291
CGTGCGA60650.013.7829419
TCCCCTA79300.013.6577682
CCTAGGC84650.013.5767538
TACCTAG84350.013.4544796
ACTCGCG90100.013.42021434-35
Produced by FastQC (version 0.11.2)