##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 m48-3.3420000000cd17.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11989871 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.328503367550827 33.0 33.0 33.0 22.0 33.0 2 29.379110751066463 33.0 33.0 33.0 15.0 33.0 3 29.377969037364956 33.0 33.0 33.0 15.0 33.0 4 32.788175452429805 37.0 37.0 37.0 15.0 37.0 5 33.085247289149315 37.0 37.0 37.0 15.0 37.0 6 33.014815505521284 37.0 33.0 37.0 15.0 37.0 7 33.306176021410074 37.0 37.0 37.0 15.0 37.0 8 33.068645942896296 37.0 33.0 37.0 15.0 37.0 9 32.45543267312885 37.0 33.0 37.0 15.0 37.0 10-11 32.679279785412206 37.0 37.0 37.0 15.0 37.0 12-13 32.847382219541814 37.0 37.0 37.0 15.0 37.0 14-15 34.721440330759194 40.0 37.0 40.0 15.0 40.0 16-17 34.39099899406757 40.0 37.0 40.0 15.0 40.0 18-19 34.41132731953496 40.0 37.0 40.0 15.0 40.0 20-21 34.31783215182215 40.0 37.0 40.0 15.0 40.0 22-23 35.102144593549006 40.0 37.0 40.0 18.5 40.0 24-25 34.64124280403017 40.0 37.0 40.0 15.0 40.0 26-27 34.36541760958062 40.0 37.0 40.0 15.0 40.0 28-29 34.10882994487597 40.0 35.0 40.0 10.5 40.0 30-31 34.25581505422369 40.0 35.0 40.0 15.0 40.0 32-33 34.07990615578766 40.0 37.0 40.0 15.0 40.0 34-35 33.87219628968485 40.0 33.0 40.0 6.0 40.0 36-37 34.00035146333101 40.0 35.0 40.0 15.0 40.0 38-39 34.05656520407935 40.0 33.0 40.0 15.0 40.0 40-41 34.06127392863526 40.0 33.0 40.0 15.0 40.0 42-43 33.84722658817597 38.5 33.0 40.0 15.0 40.0 44-45 34.19528604603002 37.0 33.0 40.0 15.0 40.0 46-47 33.52691955568163 37.0 33.0 40.0 15.0 40.0 48-49 33.45268293545443 37.0 33.0 40.0 10.5 40.0 50-51 31.923164477749594 37.0 30.0 38.5 6.0 38.5 52-53 32.18115516005135 37.0 33.0 38.5 6.0 40.0 54-55 33.051821616763014 37.0 33.0 40.0 15.0 40.0 56-57 33.405326629452475 37.0 33.0 40.0 15.0 40.0 58-59 33.02594173031553 37.0 33.0 40.0 15.0 40.0 60-61 33.605656182622816 37.0 33.0 40.0 18.5 40.0 62-63 33.83405167578533 37.0 33.0 38.5 22.0 40.0 64-65 33.57864421560499 37.0 33.0 37.0 22.0 40.0 66-67 33.06971622130046 37.0 33.0 37.0 22.0 40.0 68-69 32.235299612481235 37.0 33.0 37.0 15.0 40.0 70-71 31.81896581706342 37.0 33.0 37.0 15.0 40.0 72-73 31.75258770507206 37.0 33.0 37.0 15.0 38.5 74-75 31.474163400089957 37.0 33.0 37.0 15.0 37.0 76-77 31.202553889028497 37.0 33.0 37.0 15.0 37.0 78-79 31.060573337277773 37.0 33.0 37.0 15.0 37.0 80-81 30.923342753228955 37.0 30.0 37.0 15.0 37.0 82-83 30.805255452706703 37.0 27.0 37.0 15.0 37.0 84-85 30.697227893444392 37.0 27.0 37.0 15.0 37.0 86-87 30.536893140885333 37.0 27.0 37.0 10.5 37.0 88-89 30.459243723306116 37.0 27.0 37.0 15.0 37.0 90-91 30.377946518357035 37.0 27.0 37.0 15.0 37.0 92-93 30.336192190891794 33.0 27.0 37.0 10.5 37.0 94-95 30.178853717441996 33.0 27.0 37.0 6.0 37.0 96-97 30.135995583271914 33.0 27.0 37.0 6.0 37.0 98-99 30.08444452821886 33.0 27.0 37.0 6.0 37.0 100-101 28.524019524480288 33.0 24.5 35.0 6.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.40784229098355596 2306 2 0.8546597619108169 2306 3 0.5689953211918457 2306 4 0.7882429091963985 2306 5 0.6286144179278566 2306 6 0.7852125562274637 2306 7 0.8442423426216763 2306 8 0.56773474271629 2306 9 0.8710455061878548 2306 10-11 0.6627701939370283 2306 12-13 0.4389786622651357 2306 14-15 0.9589412415835881 2306 16-17 0.8395044944011119 2306 18-19 0.8572214378201366 2306 20-21 0.9260975993902676 2306 22-23 0.8233135647753187 2306 24-25 1.134772802665438 2306 26-27 0.5747678999523487 2306 28-29 0.8182704722131007 2306 30-31 0.8046660722498444 2306 32-33 0.8221774628330749 2306 34-35 0.8991808721829955 2306 36-37 0.7932604062672866 2306 38-39 0.8147832247393296 2306 40-41 0.6424435777059259 2306 42-43 0.7527419099654935 2306 44-45 0.7778457955825999 2306 46-47 1.0671414821527492 2306 48-49 0.8321561024242428 2306 50-51 0.7255525316892744 2306 52-53 0.6956361461994334 2306 54-55 0.6559679936490213 2306 56-57 0.5024722396327874 2306 58-59 0.7691483527672176 2306 60-61 0.29075638850979857 2306 62-63 0.660049495783575 2306 64-65 0.8426786263639627 2306 66-67 0.9264337526488973 2306 68-69 1.2403276561277181 2306 70-71 0.9922452235809764 2306 72-73 0.939372138164682 2306 74-75 0.8774343191014644 2306 76-77 0.7391998215198754 2306 78-79 0.5989405954415439 2306 80-81 0.6463098173720709 2306 82-83 0.6361004662610021 2306 84-85 0.5916417963147076 2306 86-87 0.4900884281112994 2306 88-89 0.5299321403783637 2306 90-91 0.5440921319851881 2306 92-93 0.522636550238289 2306 94-95 0.5631811059311858 2306 96-97 0.5771127317039877 2306 98-99 0.46301175106322034 2306 100-101 0.35358220793225215 2307 1 0.30280383426281077 2307 2 0.7547295503256954 2307 3 0.5955539666910106 2307 4 0.7932226234693616 2307 5 0.6462859066972584 2307 6 0.7507693077562863 2307 7 0.8072234867988541 2307 8 0.5292782174043822 2307 9 0.9104763123764243 2307 10-11 0.7251021966730065 2307 12-13 0.39445751927046757 2307 14-15 0.8754904845908698 2307 16-17 0.7588918981993729 2307 18-19 0.7611354781511253 2307 20-21 0.7977595981651788 2307 22-23 0.7402469802120564 2307 24-25 0.9071319481490079 2307 26-27 0.38410669706555467 2307 28-29 0.7987175209243063 2307 30-31 0.7239699863605367 2307 32-33 0.7428928060722342 2307 34-35 0.7975691472811093 2307 36-37 0.7556244915573629 2307 38-39 0.7992214075440671 2307 40-41 0.597866038782314 2307 42-43 0.7233030721940636 2307 44-45 0.7321419862457361 2307 46-47 0.8446010749289314 2307 48-49 0.6881608272005124 2307 50-51 0.6301084641384023 2307 52-53 0.6715088837858971 2307 54-55 0.6589559746518319 2307 56-57 0.5579918851549621 2307 58-59 0.7571800839037337 2307 60-61 0.4064664181410862 2307 62-63 0.7408936885741895 2307 64-65 0.7430197627779549 2307 66-67 0.7476766443970959 2307 68-69 0.9696633526048615 2307 70-71 1.0135708975432713 2307 72-73 0.9083718360748101 2307 74-75 0.7402349520074942 2307 76-77 0.5653572044798523 2307 78-79 0.5876313648305427 2307 80-81 0.5382024566694952 2307 82-83 0.5493132552342743 2307 84-85 0.543267580786182 2307 86-87 0.5658259522725579 2307 88-89 0.5407731355973269 2307 90-91 0.4564257776752285 2307 92-93 0.43100979719262966 2307 94-95 0.4511724785071216 2307 96-97 0.4844725326754151 2307 98-99 0.4711207651430378 2307 100-101 0.3441687391434165 2201 1 0.3487477198352984 2201 2 0.7343701748671236 2201 3 0.6981801025868322 2201 4 0.7574091637536071 2201 5 0.6515495175820263 2201 6 0.845419963970734 2201 7 0.8870876110874732 2201 8 0.6426521351413612 2201 9 0.9963927198198945 2201 10-11 0.7909605333457321 2201 12-13 0.5549818616531113 2201 14-15 0.9285011187165111 2201 16-17 0.823128903718036 2201 18-19 0.8452437077933652 2201 20-21 0.9296555881028539 2201 22-23 0.9121708189334967 2201 24-25 1.0486928751196274 2201 26-27 0.6239056249448893 2201 28-29 0.9147166364653998 2201 30-31 0.9561781245062804 2201 32-33 0.8566749082518399 2201 34-35 0.942588847327599 2201 36-37 0.8907587427302346 2201 38-39 0.9453475252688861 2201 40-41 0.7180938016619578 2201 42-43 0.867547037919131 2201 44-45 0.9212026323314433 2201 46-47 0.9626276381653298 2201 48-49 0.8189366898162334 2201 50-51 0.5810496860368559 2201 52-53 0.6798024284054591 2201 54-55 0.7985474033971016 2201 56-57 0.7416293241877483 2201 58-59 0.8638651243867344 2201 60-61 0.7039047392742788 2201 62-63 0.8533536662418157 2201 64-65 0.9529916306374346 2201 66-67 1.0578223703126 2201 68-69 1.193841853756652 2201 70-71 1.1688463937365121 2201 72-73 0.9865746216477085 2201 74-75 1.0295081313312515 2201 76-77 0.8773554083115584 2201 78-79 0.8691544717536317 2201 80-81 0.912852670847407 2201 82-83 0.8738544204710301 2201 84-85 0.8094975440575993 2201 86-87 0.8104629916879169 2201 88-89 0.8273797108581746 2201 90-91 0.8459336885254558 2201 92-93 0.8100572559757389 2201 94-95 0.7792874234873217 2201 96-97 0.8684948919587008 2201 98-99 0.8205563221130454 2201 100-101 0.6445629776108142 1101 1 0.3258541144685623 1101 2 0.6367537497616915 1101 3 0.5662922988298469 1101 4 0.6469786815770746 1101 5 0.5304473328188308 1101 6 0.811933250676006 1101 7 0.7867022472945635 1101 8 0.713723738706534 1101 9 0.8607118602081201 1101 10-11 0.6635739789637043 1101 12-13 0.45009646690970584 1101 14-15 0.864485065810868 1101 16-17 0.6422472472516318 1101 18-19 0.6544827894037937 1101 20-21 0.7222519210119955 1101 22-23 0.8964841503260601 1101 24-25 0.8479821015542441 1101 26-27 0.6426084216737067 1101 28-29 0.7758608767192072 1101 30-31 0.8229543057600068 1101 32-33 0.7152225791970395 1101 34-35 0.7749520551895159 1101 36-37 0.8038260600037503 1101 38-39 0.8208796124897901 1101 40-41 0.6767624634128495 1101 42-43 0.7961918994438051 1101 44-45 0.9380553394344631 1101 46-47 0.7786474340709333 1101 48-49 0.6371083615026407 1101 50-51 0.5843227384267919 1101 52-53 0.6404468031130364 1101 54-55 0.7024420444497537 1101 56-57 0.7589816292860547 1101 58-59 0.7644244666324624 1101 60-61 0.8811687804480854 1101 62-63 1.0078169346883072 1101 64-65 1.0194684487679169 1101 66-67 0.9340093858067391 1101 68-69 0.8608377797471931 1101 70-71 0.8233606852777022 1101 72-73 0.7404174821727274 1101 74-75 0.6853344210825405 1101 76-77 0.6479806653111879 1101 78-79 0.6950026446595743 1101 80-81 0.6535452331121618 1101 82-83 0.6301000454479144 1101 84-85 0.6295658315989421 1101 86-87 0.5765917536802263 1101 88-89 0.6322237407189668 1101 90-91 0.5606156895371726 1101 92-93 0.5654556865545857 1101 94-95 0.5648179119343446 1101 96-97 0.6551223895027611 1101 98-99 0.6557605880859114 1101 100-101 0.5982258806103609 2304 1 0.27310429879299747 2304 2 0.5960844558293985 2304 3 0.5696249441587966 2304 4 0.5315405991679327 2304 5 0.4858712194431831 2304 6 0.550111869462242 2304 7 0.7842257819503615 2304 8 0.5133874342221958 2304 9 0.9033652699998811 2304 10-11 0.6292619506380106 2304 12-13 0.429760505843511 2304 14-15 0.759235682912248 2304 16-17 0.538433606262366 2304 18-19 0.5681338884820306 2304 20-21 0.6285729700212599 2304 22-23 0.6998036283725284 2304 24-25 0.7648884892719323 2304 26-27 0.4467454451635078 2304 28-29 0.686690515387248 2304 30-31 0.7386965322449726 2304 32-33 0.6867099810074819 2304 34-35 0.6531828139613935 2304 36-37 0.6501322912208778 2304 38-39 0.717235315193399 2304 40-41 0.629366335137064 2304 42-43 0.6865951231590017 2304 44-45 0.8239755984418267 2304 46-47 0.7359214421531703 2304 48-49 0.5768181959470624 2304 50-51 0.6331004676899497 2304 52-53 0.5865698400227757 2304 54-55 0.6062846569647533 2304 56-57 0.5885506938290419 2304 58-59 0.6555450575639838 2304 60-61 0.6113310385135051 2304 62-63 0.7783974497734789 2304 64-65 0.8000348977261709 2304 66-67 0.7744366838348142 2304 68-69 0.803057151053693 2304 70-71 0.6805983891559428 2304 72-73 0.7957843718976569 2304 74-75 0.7235589632851251 2304 76-77 0.6709604642643967 2304 78-79 0.6565603560789981 2304 80-81 0.6110621744899873 2304 82-83 0.6241164660916887 2304 84-85 0.6157619114457624 2304 86-87 0.5822144373598768 2304 88-89 0.6105298324662805 2304 90-91 0.614363213772684 2304 92-93 0.6497390412172237 2304 94-95 0.5850373561295967 2304 96-97 0.6297437885182013 2304 98-99 0.6011888603001623 2304 100-101 0.5548724059195784 2202 1 0.16499448568730912 2202 2 0.4216718585129442 2202 3 0.526514658425576 2202 4 0.5282948839167858 2202 5 0.34788122828186374 2202 6 0.5401868950085742 2202 7 0.7226896772812879 2202 8 0.45775625373443773 2202 9 0.79724816593545 2202 10-11 0.5513756835643377 2202 12-13 0.39031403417101984 2202 14-15 0.6745889407555552 2202 16-17 0.563935494599491 2202 18-19 0.502293036568183 2202 20-21 0.6013565166617312 2202 22-23 0.7495002435988525 2202 24-25 0.6277841993893603 2202 26-27 0.43648049804618694 2202 28-29 0.6272798764871794 2202 30-31 0.6639662083538767 2202 32-33 0.5669779445206373 2202 34-35 0.6243761319316121 2202 36-37 0.5474173244650657 2202 38-39 0.5791847516529742 2202 40-41 0.46578773935149087 2202 42-43 0.6005766746593793 2202 44-45 0.7236412891598079 2202 46-47 0.5675543349650525 2202 48-49 0.46616050621562266 2202 50-51 0.3592578007777334 2202 52-53 0.4331711727237675 2202 54-55 0.5668471828709869 2202 56-57 0.5112330737313968 2202 58-59 0.6037729216189263 2202 60-61 0.6786600220437933 2202 62-63 0.8050112690765587 2202 64-65 0.8143359209919439 2202 66-67 0.7717824999674932 2202 68-69 0.7364520821505778 2202 70-71 0.6205228122108188 2202 72-73 0.5645467342313033 2202 74-75 0.5381338728475775 2202 76-77 0.5767674947890669 2202 78-79 0.5610129284494079 2202 80-81 0.5191801567181002 2202 82-83 0.5425002782184443 2202 84-85 0.5297507968216344 2202 86-87 0.5943980077946875 2202 88-89 0.5803172532943748 2202 90-91 0.5655159020726543 2202 92-93 0.5424399608852113 2202 94-95 0.558709456111508 2202 96-97 0.5246737649465807 2202 98-99 0.5078376907099944 2202 100-101 0.4877373626524282 1102 1 0.16453097426420982 1102 2 0.34779570592021614 1102 3 0.4491959543021373 1102 4 0.3905444880738216 1102 5 0.2601327908673241 1102 6 0.5246335923970236 1102 7 0.5981734742162033 1102 8 0.5854703800840184 1102 9 0.6593607301904356 1102 10-11 0.5034873502480366 1102 12-13 0.2966535471223892 1102 14-15 0.5186104723487617 1102 16-17 0.3669996261682158 1102 18-19 0.3235946822290785 1102 20-21 0.48918175602575076 1102 22-23 0.6603692859443768 1102 24-25 0.5110643571335061 1102 26-27 0.3196402700430454 1102 28-29 0.47384874244391995 1102 30-31 0.5611237222977792 1102 32-33 0.45924222987858343 1102 34-35 0.4533654356066279 1102 36-37 0.35699428238760333 1102 38-39 0.3717153306846228 1102 40-41 0.3645472327205823 1102 42-43 0.3998142449499298 1102 44-45 0.5536657549896873 1102 46-47 0.23458338602183915 1102 48-49 0.3424359389882383 1102 50-51 0.22745196147545244 1102 52-53 0.3302798511762717 1102 54-55 0.3904778682488086 1102 56-57 0.40587644892401187 1102 58-59 0.4462422734662681 1102 60-61 0.6305935769249658 1102 62-63 0.7511941568398868 1102 64-65 0.5795104981066359 1102 66-67 0.6391005018616127 1102 68-69 0.5606046311027697 1102 70-71 0.470657821057312 1102 72-73 0.5277822868897921 1102 74-75 0.451498233497329 1102 76-77 0.4430864101823566 1102 78-79 0.455396937094946 1102 80-81 0.46585743496139287 1102 82-83 0.42994416248223644 1102 84-85 0.4178517438394813 1102 86-87 0.4161853161567066 1102 88-89 0.4468301332420168 1102 90-91 0.44940180773911464 1102 92-93 0.425354954372807 1102 94-95 0.49452073734042656 1102 96-97 0.430311799890557 1102 98-99 0.37972698260438165 1102 100-101 0.4756387327909941 2305 1 0.29549170748494547 2305 2 0.4037302890820307 2305 3 0.5511493575242206 2305 4 0.4443563624398834 2305 5 0.324399586572774 2305 6 0.6633508706384319 2305 7 0.6335752127520777 2305 8 0.5894282133563635 2305 9 0.8345736195735824 2305 10-11 0.5507111127765896 2305 12-13 0.396210025070296 2305 14-15 0.6046774497434626 2305 16-17 0.5305448623403137 2305 18-19 0.5320675076765369 2305 20-21 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-1.6220770612542346 1213 70-71 -1.4120385364335952 1213 72-73 -1.279900134317554 1213 74-75 -1.3795265536883505 1213 76-77 -1.2451179114312403 1213 78-79 -1.1496672879774081 1213 80-81 -1.0266323359705325 1213 82-83 -1.012743658425304 1213 84-85 -1.01975994485365 1213 86-87 -0.8832928912958025 1213 88-89 -1.0158994645908948 1213 90-91 -1.0065490773479127 1213 92-93 -1.0337750317782586 1213 94-95 -0.88533347795968 1213 96-97 -0.9763093858499552 1213 98-99 -0.9355389545424018 1213 100-101 -0.8318365674410266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9596.0 3 781.0 4 480.0 5 398.0 6 549.0 7 2014.0 8 8538.0 9 69502.0 10 321998.0 11 416506.0 12 206521.0 13 113654.0 14 91780.0 15 81329.0 16 76734.0 17 73846.0 18 68365.0 19 64895.0 20 65805.0 21 68792.0 22 72809.0 23 78043.0 24 87186.0 25 102569.0 26 126295.0 27 169269.0 28 150117.0 29 145902.0 30 178427.0 31 223943.0 32 263893.0 33 351833.0 34 489543.0 35 710695.0 36 1379067.0 37 3274338.0 38 2441580.0 39 2279.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.63885429305978 12.595908409066874 13.333847885383197 46.43138941249015 2 26.507157015068962 23.131908625037216 30.172435606489227 20.1884987534046 3 24.203552567288412 28.635004763740756 23.844232316225202 23.31721035274563 4 29.10434504535646 24.185158364072212 20.271215923356067 26.439280667215257 5 29.85332270833279 25.158074166590733 18.706184296542084 26.282418828534393 6 29.43476348729511 31.3250101182263 18.847458889279224 20.392767505199366 7 29.44440293944439 15.144357404517518 35.548078163143586 19.863161492894505 8 20.45527003123273 29.174537876600297 23.19768597489177 27.1725061172752 9 24.036405582048985 27.0990500938685 25.315824126683985 23.54872019739853 10-11 28.744589293820443 31.005307403149416 17.739800231700944 22.510303071329194 12-13 29.384497327344896 19.930203976603597 23.936392206736805 26.7489064893147 14-15 27.927568702344256 21.697544343690005 24.444681972174184 25.930204981791547 16-17 28.65709998296485 23.224770443014087 23.771673887120425 24.34645568690064 18-19 29.837608111957824 22.83925037282073 24.49643366809713 22.826707847124315 20-21 28.91841128808087 25.996849758645208 23.742927075036484 21.34181187823744 22-23 34.04865531723041 22.632182591614157 21.955034346970812 21.364127744184618 24-25 30.438580963847777 25.375499516556477 22.310588665545588 21.875330854050155 26-27 23.673904057399582 30.679608553742742 21.35384648013283 24.292640908724845 28-29 27.111942718758392 28.92232573903452 22.033059451333482 21.932672090873602 30-31 32.12891544501866 23.72042902866713 22.462135200090284 21.688520326223923 32-33 29.871931369457094 23.26780701646364 24.627531489095517 22.232730124983746 34-35 27.24059691801229 27.920351366372824 21.451436281360305 23.38761543425458 36-37 24.16035622618646 25.429504283425835 27.20602181707536 23.20411767331234 38-39 23.442911912008277 22.623287277205034 28.0753322078762 25.858468602910484 40-41 30.960881247602135 21.938984125143786 22.22003741697102 24.880097210283058 42-43 32.04067429712633 21.560012787434946 24.1687405332671 22.230572382171623 44-45 33.93370562577754 22.359306714081224 22.009509130249665 21.69747852989157 46-47 28.71460490414737 22.32470536271126 22.823270750486728 26.13741898265464 48-49 26.779907075446935 22.171737715706303 21.42935038728031 29.61900482156646 50-51 28.343996570750278 21.49104636293177 23.592401302386687 26.572555763931266 52-53 25.04504512025459 24.660094360375155 26.61410880870757 23.68075171066269 54-55 22.414244403788345 23.540947835698507 26.233701877622 27.811105882891145 56-57 21.38626456946709 23.049875389584955 32.712467186035354 22.851392854912604 58-59 22.004971593873794 26.802780920099682 28.078451834814516 23.11379565121201 60-61 22.904657905640075 32.951073253167465 22.031186331669836 22.113082509522624 62-63 21.94765943447988 33.32466591701168 22.44636837899476 22.28130626951369 64-65 22.13803548458818 33.438731502331265 21.982463346283488 22.44076966679707 66-67 22.21338436783749 33.67404806546933 21.387808450290304 22.724759116402875 68-69 22.353883693365997 31.709903229611836 22.040891669110742 23.89532140791143 70-71 23.58091398629319 31.2779574653526 22.258674554785394 22.882453993568816 72-73 22.829822393788866 31.89502566013072 22.593906660345635 22.68124528573478 74-75 23.77344905819549 30.832106027527985 22.116608146470675 23.277836767805855 76-77 23.93919243476699 29.132955461528503 22.889808738946833 24.038043364757677 78-79 23.30802379675291 29.849073705040762 22.27619691146056 24.566705586745773 80-81 23.98937673339771 29.46078825984323 22.27416685845454 24.275668148304522 82-83 24.326033938940363 28.741015810470387 22.50692106219551 24.426029188393738 84-85 24.448273558794185 28.685921707947347 22.35550369668405 24.51030103657442 86-87 25.00266867541981 29.085431052761457 22.149125123242293 23.76277514857644 88-89 23.946523695384407 29.565043114053722 22.382346711315133 24.106086479246734 90-91 24.257207739522997 28.43446154966306 22.364196844844074 24.944133865969874 92-93 24.589064106066242 28.775038353571507 22.77790143015242 23.857996110209832 94-95 24.925509225359814 27.80103116379513 23.03459004307585 24.238869567769207 96-97 24.050298346415776 28.785459812456043 22.677570902928366 24.48667093819982 98-99 24.527523372914207 28.462150548953918 22.64819827399055 24.362127804141327 100-101 24.586202765757896 28.28726867320673 23.004095919565874 24.122432641469505 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 8566.0 1 4550.0 2 432.0 3 286.5 4 257.0 5 256.0 6 233.0 7 217.0 8 212.5 9 226.5 10 239.5 11 260.0 12 296.5 13 331.5 14 385.5 15 435.5 16 508.5 17 656.0 18 812.5 19 1000.0 20 1310.5 21 1751.0 22 2355.5 23 3250.0 24 4498.0 25 6308.0 26 8641.5 27 11797.0 28 16065.5 29 21496.0 30 28417.0 31 37420.5 32 48635.5 33 63452.0 34 83369.5 35 111536.0 36 146278.5 37 190939.5 38 237123.0 39 277100.0 40 321020.5 41 367505.5 42 413070.5 43 459073.0 44 508575.0 45 526498.0 46 523353.0 47 539178.5 48 524122.0 49 496860.0 50 505255.0 51 485118.0 52 435856.5 53 395646.5 54 366823.0 55 355908.5 56 360072.5 57 360128.0 58 329134.5 59 276697.5 60 248465.5 61 227554.5 62 196965.0 63 175985.5 64 153208.5 65 142307.5 66 140184.0 67 138800.0 68 129827.0 69 106572.5 70 87876.5 71 74727.5 72 58763.0 73 47399.5 74 48787.0 75 43517.5 76 27117.0 77 16679.0 78 12847.5 79 14465.5 80 13930.0 81 9106.0 82 3957.0 83 882.0 84 524.5 85 380.5 86 310.0 87 256.0 88 200.0 89 161.0 90 137.0 91 110.5 92 95.0 93 76.5 94 62.0 95 46.5 96 26.5 97 16.5 98 10.5 99 7.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15257044883969145 2 0.1272407351171668 3 0.12542253373701853 4 0.13481379407668356 5 0.14055197090944513 6 0.09580586813652958 7 0.08387079393931761 8 0.09249473993506686 9 0.1040628377069278 10-11 0.07791159721401507 12-13 0.08906267632070437 14-15 0.12227821300162445 16-17 0.12202800180252148 18-19 0.12186536452310454 20-21 0.14558121601141497 22-23 0.12341667395754301 24-25 0.11085190157592187 26-27 0.11356669308618918 28-29 0.10231552949985867 30-31 0.08239454786461005 32-33 0.10103111201113005 34-35 0.11862512949472101 36-37 0.11580191313150909 38-39 0.10932561326139371 40-41 0.1526121507062086 42-43 0.11720309584648575 44-45 0.1151138323339759 46-47 0.14862962245381955 48-49 0.15647791373234957 50-51 0.1271072891443119 52-53 0.09446306803467694 54-55 0.09237380452216708 56-57 0.09790764220899459 58-59 0.09859155281987605 60-61 0.09416281459575336 62-63 0.08455470455019909 64-65 0.08523444497442884 66-67 0.10303280160395387 68-69 0.09950065350995019 70-71 0.09606858989558771 72-73 0.10779932494686557 74-75 0.1460274259831486 76-77 0.16114435259562007 78-79 0.14930519269139758 80-81 0.12372943795642172 82-83 0.10260327237882709 84-85 0.10776596345365183 86-87 0.10805370633262026 88-89 0.10387100912094885 90-91 0.11237818989045002 92-93 0.0988084025257653 94-95 0.10046396662649665 96-97 0.09462987550074559 98-99 0.0943754941149909 100-101 0.0907099000481323 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.1989871E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.28237611590907 #Duplication Level Percentage of deduplicated Percentage of total 1 79.14902067854511 39.79800826964469 2 12.63844391509877 12.709819809176373 3 3.994154497021961 6.025067360529234 4 1.3445349945206895 2.7042565718196414 5 0.7842206064781411 1.97162377463901 6 0.4295034823937502 1.2957873386889163 7 0.3193943295426934 1.1241934065148018 8 0.1911365010076807 0.7688637946517626 9 0.1420574333221003 0.6428686763126269 >10 0.8408740022298583 7.485981554437697 >50 0.06912578125897953 2.4687207573716643 >100 0.07771563263897886 8.322011654787465 >500 0.011787216996166857 4.144823869718673 >1k 0.007609175824165498 6.933257406641767 >5k 3.212630011979007E-4 1.1279558026442387 >10k+ 1.0049011976278267E-4 2.476759952421593 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 166939 1.3923335789017246 Illumina Single End PCR Primer 1 (100% over 50bp) CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT 65001 0.5421326050964185 Illumina Single End PCR Primer 1 (100% over 31bp) CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTG 24809 0.20691632128485787 TruSeq Adapter, Index 3 (100% over 50bp) CGCGTATTTAAGTCGTCTGCAAAGGATTCGGCCCGTCGTTCGTGCGGAAT 13514 0.11271180482258733 No Hit CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA 12534 0.10453823898522344 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 1.6361226905610577 8.340373303432538E-6 0.0 2 1.6370317912511319 8.340373303432538E-6 8.340373303432538E-6 3 1.637248640957021 1.6680746606865077E-5 8.340373303432538E-6 4 1.6379325515679026 1.6680746606865077E-5 8.340373303432538E-6 5 1.6381660820203987 1.6680746606865077E-5 8.340373303432538E-6 6 1.638574760312267 1.6680746606865077E-5 8.340373303432538E-6 7 1.6389667578575282 1.6680746606865077E-5 8.340373303432538E-6 8 1.6396423280951062 1.6680746606865077E-5 8.340373303432538E-6 9 1.6401510908666157 1.6680746606865077E-5 8.340373303432538E-6 10-11 1.6421360997128325 2.5021119910297617E-5 8.340373303432538E-6 12-13 1.6442545545319045 2.5021119910297617E-5 1.2510559955148808E-5 14-15 1.6470027075353855 3.336149321373016E-5 2.5021119910297617E-5 16-17 1.6513105103466086 5.42124264723115E-5 2.9191306562013885E-5 18-19 1.6622739310539703 8.340373303432539E-5 3.336149321373015E-5 20-21 2.2964300449938118 9.174410633775793E-5 3.336149321373015E-5 22-23 2.3010881434837787 9.174410633775793E-5 4.1701866517162696E-5 24-25 2.303673659207843 1.0425466629290673E-4 6.255279977574404E-5 26-27 2.3088697117758814 1.1676522624805555E-4 6.67229864274603E-5 28-29 2.3104710634501404 1.2510559955148808E-4 6.67229864274603E-5 30-31 2.3190950094458898 1.2510559955148808E-4 6.67229864274603E-5 32-33 2.3208256369063522 1.2510559955148808E-4 6.67229864274603E-5 34-35 2.3222935426077562 1.2927578620320436E-4 7.506335973089286E-5 36-37 2.3238740433487566 1.501267194617857E-4 7.506335973089286E-5 38-39 2.387944791065725 1.501267194617857E-4 1.0008447964119047E-4 40-41 2.4561273428212864 1.501267194617857E-4 1.08424852944623E-4 42-43 2.458116521854155 1.5846709276521825E-4 1.2510559955148808E-4 44-45 2.4595218747557834 1.5846709276521825E-4 1.334459728549206E-4 46-47 2.4608271431777706 1.5846709276521825E-4 1.334459728549206E-4 48-49 2.462716237730998 1.626372794169345E-4 1.334459728549206E-4 50-51 2.4644468651914604 1.7097765272036707E-4 1.54296906113502E-4 52-53 2.466448554784284 1.8765839932723214E-4 1.626372794169345E-4 54-55 2.4691800270411584 2.0016895928238093E-4 1.6680746606865079E-4 56-57 2.4722701353500804 2.1267951923752972E-4 1.7514783937208332E-4 58-59 2.4773702736251293 2.210198925409623E-4 1.8348821267551586E-4 60-61 2.4809941658254706 2.2519007919267854E-4 1.918285859789484E-4 62-63 2.485789880474944 2.5021119910297615E-4 1.918285859789484E-4 64-65 2.5230922000745464 2.7940250566499007E-4 1.918285859789484E-4 66-67 2.5590934214388126 2.8357269231670635E-4 1.918285859789484E-4 68-69 2.5634470963032046 2.8357269231670635E-4 2.0016895928238093E-4 70-71 2.567016776077074 2.9191306562013886E-4 2.0016895928238093E-4 72-73 2.571036836009328 2.9608325227185514E-4 2.0016895928238093E-4 74-75 2.575394681060372 3.127639988787202E-4 2.0016895928238093E-4 76-77 2.583459822044791 3.419553054407341E-4 2.0433914593409721E-4 78-79 2.5901654821807507 3.419553054407341E-4 2.0850933258581347E-4 80-81 2.5975967547941092 3.5029567874416664E-4 2.16849705889246E-4 82-83 2.607413374172249 3.5029567874416664E-4 2.16849705889246E-4 84-85 2.6218505603604907 3.5863605204759915E-4 2.16849705889246E-4 86-87 2.637542972730899 3.6280623869931544E-4 2.16849705889246E-4 88-89 2.6576474425788232 3.836571719578968E-4 2.2519007919267857E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGACGGC 36015 0.0 82.132095 9 AAGACGG 38020 0.0 77.691154 8 GAAGACG 44465 0.0 67.468376 7 CAAGCAG 42515 0.0 66.1286 1 GCAGAAG 46715 0.0 63.559196 4 CGGGAGA 13010 0.0 59.994995 4 AGCGGAA 12835 0.0 59.961697 3 AAGCAGA 50500 0.0 58.99941 2 AGAAGAC 40075 0.0 58.69774 6 AGCAGAA 51610 0.0 58.617046 3 CAGAAGA 52160 0.0 57.387775 5 GATCGGG 14755 0.0 54.155594 1 TCGGGAG 13630 0.0 52.978844 3 CGGAAAG 11010 0.0 52.46791 5 ATCGGGA 14825 0.0 51.077145 2 AGATCGG 40475 0.0 49.602 1 GAGCGGC 12335 0.0 47.976284 9 ACCGGAA 12015 0.0 47.131058 3 AACCGGA 9615 0.0 44.811653 2 ATCGGAA 57280 0.0 44.811214 3 >>END_MODULE