FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 m48-3.3420000000cd17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 m48-3.3420000000cd17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11989871
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1669391.3923335789017246Illumina Single End PCR Primer 1 (100% over 50bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT650010.5421326050964185Illumina Single End PCR Primer 1 (100% over 31bp)
CAAGCAGAAGACGGCATACGAGATGCCTAAGTGACTGGAGTTCAGACGTG248090.20691632128485787TruSeq Adapter, Index 3 (100% over 50bp)
CGCGTATTTAAGTCGTCTGCAAAGGATTCGGCCCGTCGTTCGTGCGGAAT135140.11271180482258733No Hit
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA125340.10453823898522344No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGGC360150.082.1320959
AAGACGG380200.077.6911548
GAAGACG444650.067.4683767
CAAGCAG425150.066.12861
GCAGAAG467150.063.5591964
CGGGAGA130100.059.9949954
AGCGGAA128350.059.9616973
AAGCAGA505000.058.999412
AGAAGAC400750.058.697746
AGCAGAA516100.058.6170463
CAGAAGA521600.057.3877755
GATCGGG147550.054.1555941
TCGGGAG136300.052.9788443
CGGAAAG110100.052.467915
ATCGGGA148250.051.0771452
AGATCGG404750.049.6021
GAGCGGC123350.047.9762849
ACCGGAA120150.047.1310583
AACCGGA96150.044.8116532
ATCGGAA572800.044.8112143
Produced by FastQC (version 0.11.2)