##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 kx48-3.3420000000cdab.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15283528 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.059050698241926 33.0 33.0 33.0 33.0 33.0 2 31.57512113695215 33.0 33.0 33.0 27.0 33.0 3 31.6297388927478 33.0 33.0 33.0 27.0 33.0 4 35.51140737923861 37.0 37.0 37.0 33.0 37.0 5 35.693504536387145 37.0 37.0 37.0 33.0 37.0 6 35.306630183816196 37.0 37.0 37.0 33.0 37.0 7 35.63629012882366 37.0 37.0 37.0 33.0 37.0 8 35.390243011953785 37.0 37.0 37.0 33.0 37.0 9 35.182923013586915 37.0 37.0 37.0 33.0 37.0 10-11 35.4368776306099 37.0 37.0 37.0 33.0 37.0 12-13 35.71664428527235 37.0 37.0 37.0 33.0 37.0 14-15 37.72015214680799 40.0 37.0 40.0 33.0 40.0 16-17 37.78711014891326 40.0 37.0 40.0 33.0 40.0 18-19 37.85723312706333 40.0 37.0 40.0 33.0 40.0 20-21 37.66061415924386 40.0 37.0 40.0 33.0 40.0 22-23 37.58625776718569 40.0 37.0 40.0 33.0 40.0 24-25 37.714466679421136 40.0 37.0 40.0 33.0 40.0 26-27 37.71938884137223 40.0 37.0 40.0 33.0 40.0 28-29 37.555118818115815 40.0 37.0 40.0 33.0 40.0 30-31 37.54020688155248 40.0 37.0 40.0 33.0 40.0 32-33 37.54703295600335 40.0 37.0 40.0 33.0 40.0 34-35 37.33511670211223 40.0 37.0 40.0 33.0 40.0 36-37 37.388026246296015 40.0 37.0 40.0 33.0 40.0 38-39 37.34760266085161 40.0 37.0 40.0 33.0 40.0 40-41 37.26349544424559 40.0 37.0 40.0 33.0 40.0 42-43 37.182444884453375 40.0 37.0 40.0 33.0 40.0 44-45 37.07169152959971 40.0 37.0 40.0 33.0 40.0 46-47 36.93406672857209 40.0 37.0 40.0 33.0 40.0 48-49 36.79632556043343 40.0 37.0 40.0 33.0 40.0 50-51 35.390706386640574 38.5 35.0 38.5 30.0 38.5 52-53 35.72720408533946 37.0 35.0 38.5 30.0 40.0 54-55 36.40990143113554 37.0 37.0 40.0 33.0 40.0 56-57 36.364676630945425 37.0 37.0 40.0 33.0 40.0 58-59 36.2325786624659 37.0 37.0 40.0 33.0 40.0 60-61 36.0667474813407 37.0 37.0 40.0 33.0 40.0 62-63 35.89041322788822 37.0 37.0 40.0 33.0 40.0 64-65 35.72455829570241 37.0 37.0 40.0 27.0 40.0 66-67 35.49942810979245 37.0 37.0 40.0 27.0 40.0 68-69 35.29550843888924 37.0 33.0 37.0 27.0 40.0 70-71 35.07251525956572 37.0 33.0 37.0 27.0 40.0 72-73 34.850138037500244 37.0 33.0 37.0 27.0 40.0 74-75 34.631309243520214 37.0 33.0 37.0 27.0 38.5 76-77 34.429529752554515 37.0 33.0 37.0 27.0 37.0 78-79 34.26698308793624 37.0 33.0 37.0 27.0 37.0 80-81 34.13895116363185 37.0 33.0 37.0 27.0 37.0 82-83 34.03381686479719 37.0 33.0 37.0 27.0 37.0 84-85 33.927904735084724 37.0 33.0 37.0 27.0 37.0 86-87 33.82317737763166 37.0 33.0 37.0 27.0 37.0 88-89 33.728064456060146 37.0 33.0 37.0 27.0 37.0 90-91 33.6308600344109 37.0 33.0 37.0 27.0 37.0 92-93 33.55505754299662 37.0 33.0 37.0 27.0 37.0 94-95 33.478508856070405 37.0 33.0 37.0 27.0 37.0 96-97 33.401468299727654 37.0 33.0 37.0 27.0 37.0 98-99 33.33192189002435 37.0 33.0 37.0 27.0 37.0 100-101 31.6011661705334 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.4701794432359563 2306 2 0.8180466963927095 2306 3 0.7787463563288632 2306 4 0.8335406712185502 2306 5 0.681969826717193 2306 6 0.9684675240575871 2306 7 0.7361152326971947 2306 8 0.8922788019667678 2306 9 1.0301991755912283 2306 10-11 0.8375131939402607 2306 12-13 0.623382736701096 2306 14-15 1.0027837188020996 2306 16-17 0.9816593119505725 2306 18-19 0.8735465197627832 2306 20-21 0.9085014611138931 2306 22-23 1.0228111731035767 2306 24-25 0.9866696222529256 2306 26-27 0.8727110297761058 2306 28-29 0.9992515999917018 2306 30-31 0.9458807386311676 2306 32-33 0.9114741234803176 2306 34-35 1.034182779359746 2306 36-37 0.9109779108107432 2306 38-39 0.8802014285716382 2306 40-41 0.8594812518210233 2306 42-43 0.8365955211139848 2306 44-45 0.8842954006316361 2306 46-47 0.9077845392931962 2306 48-49 0.9170537121228364 2306 50-51 0.8841476584324468 2306 52-53 0.838272535615026 2306 54-55 0.878153390541641 2306 56-57 0.8584753109384877 2306 58-59 0.8330548945261924 2306 60-61 0.8766140836260448 2306 62-63 0.9107715562397516 2306 64-65 0.916615676239168 2306 66-67 0.9307439770069266 2306 68-69 0.8367584691725369 2306 70-71 0.8152944293295903 2306 72-73 0.8297926246046075 2306 74-75 0.8469505952423901 2306 76-77 0.8628955979489206 2306 78-79 0.8259906067662754 2306 80-81 0.7952718770693465 2306 82-83 0.7930945871155473 2306 84-85 0.8372660209053109 2306 86-87 0.7859170227301391 2306 88-89 0.8118130576399665 2306 90-91 0.801687112540229 2306 92-93 0.8286290272032204 2306 94-95 0.8169569534145751 2306 96-97 0.8112412259387938 2306 98-99 0.727415830172383 2306 100-101 0.6653979833682833 2307 1 0.3434591624029508 2307 2 0.7546957429800152 2307 3 0.7201675005582082 2307 4 0.7248912939374321 2307 5 0.5722051146023475 2307 6 0.8490463129614128 2307 7 0.6493559660136157 2307 8 0.8678705326670411 2307 9 0.9563161030624698 2307 10-11 0.8006677859237712 2307 12-13 0.4990794761002846 2307 14-15 0.8821858862644163 2307 16-17 0.8484131306163718 2307 18-19 0.7184529344660504 2307 20-21 0.8374840583996175 2307 22-23 0.9717874304944871 2307 24-25 0.796540200456505 2307 26-27 0.7167763019533098 2307 28-29 0.8756787684622864 2307 30-31 0.8319890787924891 2307 32-33 0.779884304328256 2307 34-35 0.8313992225829523 2307 36-37 0.7701382014189591 2307 38-39 0.741801362049685 2307 40-41 0.7358420420336387 2307 42-43 0.733886566831643 2307 44-45 0.6920487480683164 2307 46-47 0.651393215465113 2307 48-49 0.7122895896431132 2307 50-51 0.6038059593693745 2307 52-53 0.6812161455174106 2307 54-55 0.7230275166147351 2307 56-57 0.7389965828758136 2307 58-59 0.693974975352667 2307 60-61 0.7177786719127184 2307 62-63 0.7775021056331184 2307 64-65 0.7226591392931851 2307 66-67 0.6373058064526447 2307 68-69 0.6678238770951452 2307 70-71 0.695211844966984 2307 72-73 0.691698809105894 2307 74-75 0.6458336941155309 2307 76-77 0.7171907473546071 2307 78-79 0.7103843636655185 2307 80-81 0.6500186984762024 2307 82-83 0.6978838517136126 2307 84-85 0.6526943675923675 2307 86-87 0.6450528753334268 2307 88-89 0.6609575646115928 2307 90-91 0.6265461389726426 2307 92-93 0.6367169195273163 2307 94-95 0.6279996601453917 2307 96-97 0.6372346605360946 2307 98-99 0.6496824259103988 2307 100-101 0.5408293294827651 2201 1 0.32307446713073773 2201 2 0.7169658461921848 2201 3 0.6882643387496223 2201 4 0.7177680968224962 2201 5 0.5855142844077079 2201 6 0.8684960627205882 2201 7 0.6176869314430959 2201 8 0.8074218141153011 2201 9 0.9534197302459759 2201 10-11 0.7741471259351087 2201 12-13 0.5539082134742515 2201 14-15 0.8720124412273194 2201 16-17 0.8161628764581152 2201 18-19 0.8130314835758625 2201 20-21 0.9069292790430694 2201 22-23 0.9415499498116873 2201 24-25 0.8090889072206195 2201 26-27 0.7528133650035045 2201 28-29 0.8983039384476612 2201 30-31 0.8941350917419228 2201 32-33 0.8617616185992887 2201 34-35 0.931643432679337 2201 36-37 0.8334549168061898 2201 38-39 0.8334296394368437 2201 40-41 0.7451720336701229 2201 42-43 0.773389244751364 2201 44-45 0.7919838300068918 2201 46-47 0.7919332485861688 2201 48-49 0.8076511745006201 2201 50-51 0.47836285352043006 2201 52-53 0.7002025077780374 2201 54-55 0.911210181438662 2201 56-57 0.8606483529253381 2201 58-59 0.8730280823370933 2201 60-61 0.860712008143274 2201 62-63 0.7953536715610809 2201 64-65 0.8208902005120322 2201 66-67 0.8633266340252135 2201 68-69 0.8660137898291822 2201 70-71 0.9015325825887928 2201 72-73 0.9053636525108928 2201 74-75 0.9098284739949847 2201 76-77 0.8246770642799106 2201 78-79 0.837699436293228 2201 80-81 0.8870328404026608 2201 82-83 0.9209815734248679 2201 84-85 0.8712806793106225 2201 86-87 0.8200969177266657 2201 88-89 0.8830746212525753 2201 90-91 0.8805714717235347 2201 92-93 0.8337812973189074 2201 94-95 0.8035687663912015 2201 96-97 0.8282027028217414 2201 98-99 0.8076777019389141 2201 100-101 0.7383908897933829 1101 1 0.32744036219921213 1101 2 0.7152200110744822 1101 3 0.6495816845412996 1101 4 0.6983699420068135 1101 5 0.5547510154536397 1101 6 0.8180697769942995 1101 7 0.5843971159173478 1101 8 0.8148638119750373 1101 9 0.9538197014986949 1101 10-11 0.7535309604954463 1101 12-13 0.5360182704951839 1101 14-15 0.9103117725706724 1101 16-17 0.8032823764172505 1101 18-19 0.7724178669109918 1101 20-21 0.878302396797082 1101 22-23 0.9797183214296439 1101 24-25 0.8712281741225496 1101 26-27 0.7590424856819453 1101 28-29 0.8809604725335518 1101 30-31 0.8643729670946243 1101 32-33 0.7947024502109912 1101 34-35 0.8915794314485552 1101 36-37 0.8562958580002658 1101 38-39 0.8108874673397821 1101 40-41 0.7993476684125582 1101 42-43 0.8286486963690507 1101 44-45 0.8156352812192935 1101 46-47 0.7587414558523378 1101 48-49 0.7892320997535975 1101 50-51 0.6972082973495475 1101 52-53 0.7474632312505562 1101 54-55 0.8460661606772817 1101 56-57 0.915877974093938 1101 58-59 0.8889197314046768 1101 60-61 0.9493034405741057 1101 62-63 0.9321379094305513 1101 64-65 0.909853349763516 1101 66-67 0.8549906655701847 1101 68-69 0.8158953056904394 1101 70-71 0.838487953130489 1101 72-73 0.8594922882397853 1101 74-75 0.862946163137913 1101 76-77 0.9155982916794088 1101 78-79 0.9105294369229924 1101 80-81 0.8902032081418128 1101 82-83 0.8356296712830229 1101 84-85 0.8817671457473963 1101 86-87 0.8053514611901065 1101 88-89 0.8892867701409202 1101 90-91 0.8705774832718731 1101 92-93 0.8606954544692442 1101 94-95 0.9164181478396003 1101 96-97 0.8872408191939058 1101 98-99 0.9273654257384507 1101 100-101 0.9080514254775558 2304 1 0.3277470290254527 2304 2 0.6326421890976697 2304 3 0.5974700049424264 2304 4 0.5900129196816266 2304 5 0.4128533610093541 2304 6 0.6918647886203502 2304 7 0.5597251759937194 2304 8 0.7130316865441486 2304 9 0.8698667662629376 2304 10-11 0.6329647410222776 2304 12-13 0.44340703929945136 2304 14-15 0.7389158316324895 2304 16-17 0.6404392843156685 2304 18-19 0.5786897663760371 2304 20-21 0.6906776208538759 2304 22-23 0.7896969687153117 2304 24-25 0.6683354739249339 2304 26-27 0.5941064685441759 2304 28-29 0.683801781060005 2304 30-31 0.6894894110009915 2304 32-33 0.6322065259889982 2304 34-35 0.6779970706204921 2304 36-37 0.6486574311444144 2304 38-39 0.6399877656446478 2304 40-41 0.6211174382039744 2304 42-43 0.5923822518642865 2304 44-45 0.6402883237398029 2304 46-47 0.6448172276828998 2304 48-49 0.615308419782572 2304 50-51 0.5605165898543234 2304 52-53 0.5892102237838586 2304 54-55 0.6394629100150979 2304 56-57 0.6741699769986198 2304 58-59 0.6905682566930267 2304 60-61 0.7232445779622836 2304 62-63 0.6442355134560245 2304 64-65 0.6306766125246668 2304 66-67 0.6581359346757694 2304 68-69 0.6193922942666745 2304 70-71 0.6369259659807156 2304 72-73 0.7134016069565519 2304 74-75 0.6977821765309429 2304 76-77 0.6901903374748244 2304 78-79 0.6935040541781987 2304 80-81 0.6831414396923279 2304 82-83 0.6803294303459637 2304 84-85 0.6782553133478686 2304 86-87 0.6591775386798844 2304 88-89 0.6902135111438099 2304 90-91 0.6854334557198811 2304 92-93 0.6764618956925261 2304 94-95 0.6857865151695961 2304 96-97 0.6101868992470898 2304 98-99 0.5567950754744047 2304 100-101 0.5792542307092212 2202 1 0.2083394014357367 2202 2 0.5830932165046114 2202 3 0.5687997573472465 2202 4 0.5808771003469673 2202 5 0.36988153579572014 2202 6 0.6356888175064555 2202 7 0.5357362131989447 2202 8 0.6913771892245464 2202 9 0.8632161670599885 2202 10-11 0.6386831545005762 2202 12-13 0.4444852336352838 2202 14-15 0.7408079727466728 2202 16-17 0.6785403715486922 2202 18-19 0.5514654904129301 2202 20-21 0.6944629983234734 2202 22-23 0.8068557539632337 2202 24-25 0.6495842609158231 2202 26-27 0.5810773787014227 2202 28-29 0.6755798168802798 2202 30-31 0.6683272893891754 2202 32-33 0.6296547081240789 2202 34-35 0.7504562934938406 2202 36-37 0.623381871982545 2202 38-39 0.6280923388067663 2202 40-41 0.585552040525414 2202 42-43 0.6066513667680553 2202 44-45 0.5891373194153644 2202 46-47 0.599581285634109 2202 48-49 0.5797634607186879 2202 50-51 0.4332065023292344 2202 52-53 0.5325233815174428 2202 54-55 0.6818405256396005 2202 56-57 0.7387566176464375 2202 58-59 0.7154339659853761 2202 60-61 0.7023121625902746 2202 62-63 0.6884142945114107 2202 64-65 0.7554670916985913 2202 66-67 0.7364822205125279 2202 68-69 0.7014041817559615 2202 70-71 0.6449912929985473 2202 72-73 0.6774862384844269 2202 74-75 0.6858879391485928 2202 76-77 0.7010391435441079 2202 78-79 0.670851550668722 2202 80-81 0.6880228827727635 2202 82-83 0.7221579944729228 2202 84-85 0.7606659569728507 2202 86-87 0.7380004605479655 2202 88-89 0.7415038466409314 2202 90-91 0.737858567128292 2202 92-93 0.7159450360435713 2202 94-95 0.7134794071717465 2202 96-97 0.6828673137428964 2202 98-99 0.6616452747376584 2202 100-101 0.6713482495143985 1102 1 0.19561119521964088 1102 2 0.5566816053888424 1102 3 0.4999757643543923 1102 4 0.5048824116928969 1102 5 0.31693539830543926 1102 6 0.6160139766058137 1102 7 0.47140257778831796 1102 8 0.6538515651737526 1102 9 0.7509744602585897 1102 10-11 0.5856974256361909 1102 12-13 0.3794157449561979 1102 14-15 0.6517140754300712 1102 16-17 0.5314488595735511 1102 18-19 0.4667238981448705 1102 20-21 0.612406864138137 1102 22-23 0.7245784573036502 1102 24-25 0.5751758648927776 1102 26-27 0.4679017122932265 1102 28-29 0.5573898849166028 1102 30-31 0.5746108522299735 1102 32-33 0.5150320032221742 1102 34-35 0.6489358540168695 1102 36-37 0.5073718863867782 1102 38-39 0.5159397558255421 1102 40-41 0.5358265377885303 1102 42-43 0.4978503640280678 1102 44-45 0.5452366254253818 1102 46-47 0.41342711979508806 1102 48-49 0.5238274755012426 1102 50-51 0.40143511772360796 1102 52-53 0.417106226120481 1102 54-55 0.49420920501695775 1102 56-57 0.5206063907696006 1102 58-59 0.5417765862962654 1102 60-61 0.5890889214706974 1102 62-63 0.6048581780042994 1102 64-65 0.5295360153543527 1102 66-67 0.5852233918324643 1102 68-69 0.5303406598536213 1102 70-71 0.5480777549325921 1102 72-73 0.5512652245635437 1102 74-75 0.5770274863365117 1102 76-77 0.6068517744778745 1102 78-79 0.5843609745001288 1102 80-81 0.6043230603334351 1102 82-83 0.5619846384730067 1102 84-85 0.564900925720373 1102 86-87 0.5730854447557263 1102 88-89 0.5949532321420037 1102 90-91 0.6480045060503485 1102 92-93 0.5933339178361621 1102 94-95 0.6064914044981577 1102 96-97 0.5548638703103208 1102 98-99 0.5601861551452387 1102 100-101 0.6671995576656791 2305 1 0.26911161628121505 2305 2 0.5309397498691872 2305 3 0.5554685719479941 2305 4 0.48427555513620746 2305 5 0.3409218518674706 2305 6 0.674773244333501 2305 7 0.4406715799098677 2305 8 0.6446575076395007 2305 9 0.7868045512928532 2305 10-11 0.5602716005679085 2305 12-13 0.37654542786059153 2305 14-15 0.5573223313605382 2305 16-17 0.5284450162934107 2305 18-19 0.5144979177086171 2305 20-21 0.5961760571117765 2305 22-23 0.6461831251522767 2305 24-25 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1213 78-79 -1.2841682040761455 1213 80-81 -1.1668686718448456 1213 82-83 -1.0838022472819944 1213 84-85 -1.1800853853190887 1213 86-87 -1.1459988414943965 1213 88-89 -1.2707795530145134 1213 90-91 -1.2470902382385205 1213 92-93 -1.3322730744861957 1213 94-95 -1.1295702150162015 1213 96-97 -1.304555864885664 1213 98-99 -1.203683921341181 1213 100-101 -1.080854697093077 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12418.0 3 999.0 4 626.0 5 475.0 6 461.0 7 1012.0 8 2214.0 9 8100.0 10 28292.0 11 37464.0 12 27725.0 13 25862.0 14 27147.0 15 30521.0 16 34876.0 17 40013.0 18 44879.0 19 50404.0 20 55769.0 21 61836.0 22 69277.0 23 76840.0 24 85878.0 25 97123.0 26 113055.0 27 135559.0 28 152613.0 29 177157.0 30 215228.0 31 269437.0 32 337933.0 33 454148.0 34 639066.0 35 959704.0 36 1912587.0 37 4779863.0 38 4312472.0 39 4495.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.131838702377856 13.319871483966722 14.671908543831192 49.876381269824236 2 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24.915057944017555 25.126032934213622 25.349339609598825 24.609569512169998 46-47 24.695471357135474 25.075704988059478 25.25883468413273 24.969988970672315 48-49 24.310721410174153 25.20956495887048 25.163431685121452 25.316281945833918 50-51 24.341701525672367 25.22919349924012 25.554578068612898 24.87452690647461 52-53 24.479779948350814 25.286347389008647 25.47518370656881 24.758688956071733 54-55 24.147466207393613 25.274497132517837 25.47811480833051 25.09992185175804 56-57 24.02963861369165 25.25501630333994 26.02924033466834 24.68610474830007 58-59 24.383632244893068 25.356669718996784 25.45538195705469 24.804316079055457 60-61 24.204166015693627 25.59578728125464 25.34950416402798 24.850542539023753 62-63 24.228641020143517 25.71666792619042 25.431837652146434 24.622853401519627 64-65 24.29597588147045 25.73742893944572 25.097878220862253 24.868716958221576 66-67 24.22402084110992 25.69446950673119 25.11537075740607 24.966138894752827 68-69 24.2727379218327 25.68743213149014 25.272241165030117 24.767588781647042 70-71 24.475914141623857 25.60001954339452 25.053420139887216 24.870646175094407 72-73 24.355312083151386 25.642911174992307 25.23538063230853 24.766396109547777 74-75 24.576433923737973 25.54205884807353 25.232088139448717 24.649419088739783 76-77 24.74814403379161 25.4911760706111 25.03817776969471 24.722502125902587 78-79 24.461135847576728 25.470632223563378 25.178615491939116 24.88961643692078 80-81 24.597005350725585 25.550288726396026 25.085550715214755 24.767155207663635 82-83 24.571436899140114 25.53528529745568 24.9355352083363 24.957742595067902 84-85 24.45851531730894 25.446938932314247 25.05035311206763 25.044192638309188 86-87 24.52538951843795 25.698383413288926 25.090583569661913 24.68564349861121 88-89 24.68660934978166 25.57032235000233 24.9617924913575 24.781275808858506 90-91 24.715167718762483 25.356457333252763 25.053384302949883 24.874990645034874 92-93 24.646570783219676 25.58669847637413 25.227678349063474 24.539052391342715 94-95 24.6242978175472 25.4495845828139 25.084140069673882 24.84197752996502 96-97 24.43513930502135 25.56293682993213 25.06246994472531 24.939453920321206 98-99 24.655304117017035 25.41174607502345 25.155622233346588 24.777327574612926 100-101 24.810386604964883 25.409570238017192 25.00366911276367 24.776374044254247 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11082.0 1 5908.0 2 563.5 3 371.5 4 323.0 5 299.0 6 301.0 7 304.5 8 322.5 9 332.0 10 343.5 11 389.0 12 446.0 13 500.0 14 577.5 15 695.5 16 848.5 17 1014.0 18 1297.5 19 1723.5 20 2258.5 21 3104.0 22 4307.5 23 6069.0 24 8769.5 25 12540.5 26 17559.5 27 24277.5 28 33081.5 29 44502.5 30 58380.5 31 75227.5 32 95731.0 33 120353.0 34 150876.0 35 190942.5 36 238921.5 37 297433.5 38 362495.5 39 426254.5 40 489554.5 41 547509.5 42 595793.5 43 632338.0 44 658077.0 45 657257.5 46 638177.5 47 622245.5 48 588980.5 49 549088.5 50 526196.5 51 496313.0 52 461547.0 53 437432.0 54 418734.5 55 406265.5 56 402891.5 57 398332.5 58 375702.5 59 338223.5 60 311850.5 61 295655.0 62 273393.0 63 248801.5 64 218949.5 65 196878.0 66 190801.0 67 183946.0 68 170772.0 69 149038.0 70 125127.0 71 104046.0 72 83900.5 73 70701.5 74 60714.5 75 48569.0 76 34414.0 77 22370.0 78 15940.5 79 14213.5 80 11708.0 81 7591.0 82 3708.0 83 1287.0 84 781.5 85 542.5 86 402.0 87 323.5 88 282.0 89 219.5 90 169.5 91 155.5 92 121.0 93 93.0 94 69.0 95 51.0 96 39.0 97 23.5 98 13.5 99 9.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15774499186313526 2 0.1318936308423029 3 0.13046071561487635 4 0.13986299498388066 5 0.14579094565076858 6 0.09737280554594463 7 0.08555616216360516 8 0.0946967218563672 9 0.10588523801572516 10-11 0.07932396237308559 12-13 0.09098030245372665 14-15 0.1247323262011232 16-17 0.12419907236077954 18-19 0.12392426670072512 20-21 0.1485913461865611 22-23 0.12598530915113318 24-25 0.11287969636330042 26-27 0.11616100680418813 28-29 0.10434109192589565 30-31 0.08399893008996352 32-33 0.10329748471687951 34-35 0.12027327721714515 36-37 0.11818606279911288 38-39 0.11220249670102347 40-41 0.1560241849918422 42-43 0.11941941677340467 44-45 0.1176070080154268 46-47 0.1524844263706652 48-49 0.16014954138861132 50-51 0.13018590995482196 52-53 0.096502587622439 54-55 0.09468036437660204 56-57 0.10035313835915372 58-59 0.10083404826424892 60-61 0.09630302636930425 62-63 0.08640675111139261 64-65 0.08687130353672268 66-67 0.1047598434078833 68-69 0.10137384509649866 70-71 0.09776538506030806 72-73 0.11004985236393063 74-75 0.14978544220941656 76-77 0.16555732419896768 78-79 0.15339063074965412 80-81 0.12651856299147685 82-83 0.10524729630488458 84-85 0.11021669865753508 86-87 0.11008256732346093 88-89 0.1062647315462765 90-91 0.11496363928537967 92-93 0.10104015250928973 94-95 0.10261701355864955 96-97 0.09674140682701009 98-99 0.09646005817504963 100-101 0.09270765231692578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5283528E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.08487584148193 #Duplication Level Percentage of deduplicated Percentage of total 1 77.9024000995451 44.470488374359824 2 13.478035762976944 15.387839962331839 3 3.7054808910809394 6.345807498010178 4 1.6345759229953245 3.7323825447065535 5 0.9121653421946933 2.6035422653043474 6 0.5021121041292679 1.7197804273634705 7 0.3751473480373405 1.4990667849480954 8 0.2691325815748511 1.2290720003278306 9 0.1914343665801321 0.983520634321762 >10 0.9027314467608047 9.1288336189875 >50 0.06805034841687728 2.7136414211532607 >100 0.05188969527498231 5.897353213016324 >500 0.004489232121396962 1.7700137202341597 >1k 0.0023073104440799693 2.2396678112113606 >5k 3.604437574216391E-5 0.13710187353686387 >10k+ 1.1503491496859764E-5 0.14188785018669398 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 21607 0.1413744261141799 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.15915827811484365 3.271495953028646E-5 6.542991906057292E-6 2 0.16186707676395137 3.271495953028646E-5 1.9628975718171876E-5 3 0.16202410856969673 3.925795143634375E-5 1.9628975718171876E-5 4 0.16261952083314796 3.925795143634375E-5 1.9628975718171876E-5 5 0.16277655263889332 4.580094334240105E-5 1.9628975718171876E-5 6 0.16326727703184762 5.8886927154515636E-5 2.617196762422917E-5 7 0.1634373948214051 6.542991906057293E-5 2.617196762422917E-5 8 0.16372528646527162 7.85159028726875E-5 3.271495953028646E-5 9 0.16402626409295026 7.85159028726875E-5 3.271495953028646E-5 10-11 0.1646674772997439 9.81448785908594E-5 3.271495953028646E-5 12-13 0.16530214751463146 1.0795936644994533E-4 3.925795143634375E-5 14-15 0.16604804859192196 1.3085983812114585E-4 4.580094334240105E-5 16-17 0.16684629360446096 1.472173178862891E-4 5.234393524845834E-5 18-19 0.1675627512181742 1.5048881383931774E-4 5.561543120148699E-5 20-21 0.16929664407327943 1.7338928551051825E-4 5.8886927154515636E-5 22-23 0.1708146181954847 1.897467652756615E-4 7.197291096663021E-5 24-25 0.17143620242656016 2.0283274908777606E-4 9.81448785908594E-5 26-27 0.1724111082205627 2.0283274908777606E-4 1.1777385430903126E-4 28-29 0.17308176489093358 2.0283274908777606E-4 1.3740283002720315E-4 30-31 0.1741940735149633 2.2900471671200525E-4 1.5048881383931774E-4 32-33 0.17506756293442194 2.4536219647714846E-4 1.766607814635469E-4 34-35 0.17564988921406105 2.551766843362344E-4 2.257332207589766E-4 36-37 0.17736088159749502 2.68262668148349E-4 2.3881920457109116E-4 38-39 0.17843066077413539 2.7153416410137765E-4 2.4536219647714846E-4 40-41 0.1792550777542986 2.748056600544063E-4 2.5844818028926305E-4 42-43 0.1804262733054829 2.944346357725782E-4 2.7153416410137765E-4 44-45 0.1811623598949143 3.009776276786355E-4 3.0097762767863543E-4 46-47 0.18192788994792303 3.075206195846927E-4 3.1406361149075E-4 48-49 0.18275230692808625 3.1406361149075E-4 3.271495953028646E-4 50-51 0.1847610054432458 3.2387809934983597E-4 3.336925872089219E-4 52-53 0.18707395308203706 3.3696408316195056E-4 3.4350707506800786E-4 54-55 0.1895308465427616 3.5005006697406515E-4 3.664075467392084E-4 56-57 0.1904697658812808 3.5659305888012245E-4 3.860365224573803E-4 58-59 0.1917816357584453 3.598645548331511E-4 3.9257951436343753E-4 60-61 0.19286450091889779 3.6313605078617975E-4 4.056654981755521E-4 62-63 0.19494844384097704 3.79493530551323E-4 4.122084900816094E-4 64-65 0.195972422074275 3.925795143634376E-4 4.122084900816094E-4 66-67 0.1973562648624061 4.0893699412858077E-4 4.2856596984675266E-4 68-69 0.1987008496991009 4.1547998603463807E-4 4.4165195365886725E-4 70-71 0.20005852051960776 4.383804577058386E-4 4.5473793747098185E-4 72-73 0.20154705117823582 4.580094334240105E-4 4.7763840914218233E-4 74-75 0.203853455825121 4.678239212830964E-4 4.841814010482396E-4 76-77 0.20550228978544743 4.841814010482396E-4 4.907243929542969E-4 78-79 0.20751098830060705 4.874528970012683E-4 5.005388808133829E-4 80-81 0.20984029341916344 5.234393524845834E-4 5.299823443906407E-4 82-83 0.21276828229712408 5.332538403436694E-4 5.757832877330418E-4 84-85 0.21656648909859033 5.463398241557839E-4 5.888692715451564E-4 86-87 0.22050864172198986 5.790547836860704E-4 5.954122634512137E-4 88-89 0.2251737949510087 5.954122634512137E-4 6.117697432163568E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGCG 4680 0.0 24.15844 4 CGCGTAT 3620 0.0 24.145155 1 CATGCGT 4840 0.0 23.16344 5 ATCGGAA 11595 0.0 22.492588 3 CCGTATC 7155 0.0 22.177853 48-49 CGCCGTA 7430 0.0 20.945524 46-47 TCGTGTA 6970 0.0 20.413136 14-15 CGCGTGT 2450 0.0 20.359217 3 GCCGTAT 6445 0.0 20.308664 48-49 GTAGGGA 7225 0.0 20.021988 18-19 AGAGCGT 11525 0.0 19.987783 9 GAGTGTA 6285 0.0 19.948908 28-29 AGATCGG 12235 0.0 19.762167 1 TAAGTCG 4460 0.0 19.488573 9 CGTATCA 6690 0.0 19.239614 50-51 GCGTATT 4505 0.0 18.978544 2 GATCGGA 13955 0.0 18.92475 2 CGTGTAG 6580 0.0 18.62731 16-17 GTCGTGT 6705 0.0 18.421299 14-15 TTAAAAA 20050 0.0 18.249083 56-57 >>END_MODULE