FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 kx48-3.3420000000cdab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 kx48-3.3420000000cdab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15283528
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC216070.1413744261141799Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGCG46800.024.158444
CGCGTAT36200.024.1451551
CATGCGT48400.023.163445
ATCGGAA115950.022.4925883
CCGTATC71550.022.17785348-49
CGCCGTA74300.020.94552446-47
TCGTGTA69700.020.41313614-15
CGCGTGT24500.020.3592173
GCCGTAT64450.020.30866448-49
GTAGGGA72250.020.02198818-19
AGAGCGT115250.019.9877839
GAGTGTA62850.019.94890828-29
AGATCGG122350.019.7621671
TAAGTCG44600.019.4885739
CGTATCA66900.019.23961450-51
GCGTATT45050.018.9785442
GATCGGA139550.018.924752
CGTGTAG65800.018.6273116-17
GTCGTGT67050.018.42129914-15
TTAAAAA200500.018.24908356-57
Produced by FastQC (version 0.11.2)