FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 kx24-3.3420000000cbad.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 kx24-3.3420000000cbad.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25266941
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT67850.012.7431731
CTCGAAT115600.011.7534191
CTCGGAT129750.011.277151
CTCGACT125050.011.1311471
CGGGAAT71950.010.6964641
CGCAAAT62700.010.4560481
CTGGAAT232000.010.4023571
CGAGAAT97950.010.2337321
CTCGATT134450.010.2115831
CTCGGTT109250.010.0014371
CGCGAAT28700.09.931741
CTCAGAT212450.09.7495771
TTACTCG71450.09.7077323
CTCGTTT109650.09.5750191
CCCGAAT57150.09.5595541
CCGAAAT76900.09.51372051
CGGAAAT82800.09.4669411
CGGAGAT226600.09.3713821
CTGGGAT171800.09.318861
CGGCAAT93800.09.2683891
Produced by FastQC (version 0.11.2)