##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 kx24-3.3420000000cbad.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 25266941 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18424636365756 33.0 33.0 33.0 33.0 33.0 2 31.723687445979312 33.0 33.0 33.0 27.0 33.0 3 31.795950249774993 33.0 33.0 33.0 27.0 33.0 4 35.70248654160391 37.0 37.0 37.0 33.0 37.0 5 35.88408458309219 37.0 37.0 37.0 33.0 37.0 6 35.477328973064054 37.0 37.0 37.0 33.0 37.0 7 35.821058987710465 37.0 37.0 37.0 33.0 37.0 8 35.57414480842774 37.0 37.0 37.0 33.0 37.0 9 35.38501368250316 37.0 37.0 37.0 33.0 37.0 10-11 35.631216161069915 37.0 37.0 37.0 33.0 37.0 12-13 35.912910114445594 37.0 37.0 37.0 33.0 37.0 14-15 37.933161616200394 40.0 37.0 40.0 33.0 40.0 16-17 38.007332585293966 40.0 37.0 40.0 33.0 40.0 18-19 38.07985331504909 40.0 37.0 40.0 33.0 40.0 20-21 37.88113016925951 40.0 37.0 40.0 33.0 40.0 22-23 37.75866160450527 40.0 37.0 40.0 33.0 40.0 24-25 37.91987209690322 40.0 37.0 40.0 33.0 40.0 26-27 37.935667479494256 40.0 37.0 40.0 33.0 40.0 28-29 37.783645891285374 40.0 37.0 40.0 33.0 40.0 30-31 37.77721290044569 40.0 37.0 40.0 33.0 40.0 32-33 37.79288537540022 40.0 37.0 40.0 33.0 40.0 34-35 37.57844479472209 40.0 37.0 40.0 33.0 40.0 36-37 37.62757412937324 40.0 37.0 40.0 33.0 40.0 38-39 37.58313198657487 40.0 37.0 40.0 33.0 40.0 40-41 37.496821142693925 40.0 37.0 40.0 33.0 40.0 42-43 37.42672702247573 40.0 37.0 40.0 33.0 40.0 44-45 37.29911958871476 40.0 37.0 40.0 33.0 40.0 46-47 37.195006015963706 40.0 37.0 40.0 33.0 40.0 48-49 37.04835955409086 40.0 37.0 40.0 33.0 40.0 50-51 35.648396495642274 38.5 35.0 38.5 30.0 38.5 52-53 35.98558060510768 37.0 37.0 38.5 33.0 40.0 54-55 36.665721406481296 37.0 37.0 40.0 33.0 40.0 56-57 36.607219152488625 37.0 37.0 40.0 33.0 40.0 58-59 36.482244031835904 37.0 37.0 40.0 33.0 40.0 60-61 36.281369102021486 37.0 37.0 40.0 33.0 40.0 62-63 36.08860075305515 37.0 37.0 40.0 33.0 40.0 64-65 35.92932892034695 37.0 37.0 40.0 33.0 40.0 66-67 35.732966329402515 37.0 37.0 40.0 33.0 40.0 68-69 35.55489368499337 37.0 37.0 37.0 33.0 40.0 70-71 35.335758056347224 37.0 35.0 37.0 33.0 40.0 72-73 35.11112219718248 37.0 33.0 37.0 27.0 40.0 74-75 34.8811413102995 37.0 33.0 37.0 27.0 38.5 76-77 34.67589665880013 37.0 33.0 37.0 27.0 37.0 78-79 34.5110901632295 37.0 33.0 37.0 27.0 37.0 80-81 34.3755100191986 37.0 33.0 37.0 27.0 37.0 82-83 34.2655142939543 37.0 33.0 37.0 27.0 37.0 84-85 34.15646688295192 37.0 33.0 37.0 27.0 37.0 86-87 34.05440181698291 37.0 33.0 37.0 27.0 37.0 88-89 33.963835590544974 37.0 33.0 37.0 27.0 37.0 90-91 33.86908001645312 37.0 33.0 37.0 27.0 37.0 92-93 33.7880337987887 37.0 33.0 37.0 27.0 37.0 94-95 33.710170851311204 37.0 33.0 37.0 27.0 37.0 96-97 33.63079768936018 37.0 33.0 37.0 27.0 37.0 98-99 33.56616717077069 37.0 33.0 37.0 27.0 37.0 100-101 31.810062444836518 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.4443609458524449 2306 2 0.8161162619377897 2306 3 0.7654524139879264 2306 4 0.8004354744849422 2306 5 0.6216765212688813 2306 6 0.9333783564010716 2306 7 0.69936216203768 2306 8 0.9117927968225104 2306 9 1.0311617352819056 2306 10-11 0.8422120233944739 2306 12-13 0.6167449103084834 2306 14-15 0.9887603845214912 2306 16-17 0.9689778312174369 2306 18-19 0.8487008863703167 2306 20-21 0.9015997475086408 2306 22-23 1.025309609409419 2306 24-25 0.9555067353846454 2306 26-27 0.8993634608095462 2306 28-29 0.9783173324674124 2306 30-31 0.9225474899019801 2306 32-33 0.9165557073012494 2306 34-35 1.0483186241544828 2306 36-37 0.9312833872279782 2306 38-39 0.8827903705212279 2306 40-41 0.8923474853531559 2306 42-43 0.8769749744615396 2306 44-45 0.8909473606578047 2306 46-47 0.9054290905636506 2306 48-49 0.9120077972182585 2306 50-51 0.8542210439649551 2306 52-53 0.8679768813132398 2306 54-55 0.9374681163511909 2306 56-57 0.9076100219439027 2306 58-59 0.8901543423503142 2306 60-61 0.9355946397805894 2306 62-63 0.9464589059270665 2306 64-65 0.9075686618317675 2306 66-67 0.9356895526310183 2306 68-69 0.8914732178212077 2306 70-71 0.8641785383612302 2306 72-73 0.9159841654729846 2306 74-75 0.8518869136843961 2306 76-77 0.8651977055033768 2306 78-79 0.854878923422909 2306 80-81 0.8453994578780311 2306 82-83 0.8583779668281224 2306 84-85 0.8958142593124236 2306 86-87 0.8539284469547965 2306 88-89 0.9118049855358308 2306 90-91 0.9207141456038315 2306 92-93 0.9519271735784898 2306 94-95 0.9283869857645257 2306 96-97 0.9236862423120655 2306 98-99 0.8664998138749098 2306 100-101 0.7747715777228983 2307 1 0.32358872814949535 2307 2 0.7297391778890336 2307 3 0.7063162899222029 2307 4 0.7003498633431846 2307 5 0.5676059007647964 2307 6 0.8446851866827529 2307 7 0.591690371265635 2307 8 0.8011602892168526 2307 9 0.8990550120414298 2307 10-11 0.7469324080377149 2307 12-13 0.4707965333485049 2307 14-15 0.8543688720700189 2307 16-17 0.7918621233818541 2307 18-19 0.7025311308669799 2307 20-21 0.8143379601538214 2307 22-23 0.9484895088971186 2307 24-25 0.7571825363407143 2307 26-27 0.6546493851509254 2307 28-29 0.8746677644977154 2307 30-31 0.7766944964625324 2307 32-33 0.7634827675989726 2307 34-35 0.799891882285884 2307 36-37 0.7659172701184147 2307 38-39 0.7698821795940276 2307 40-41 0.7514679712624996 2307 42-43 0.723140390229581 2307 44-45 0.7152809632449859 2307 46-47 0.6515150262202312 2307 48-49 0.725766732393275 2307 50-51 0.6280997743011056 2307 52-53 0.7269582119734466 2307 54-55 0.7604905900523633 2307 56-57 0.7742166939385697 2307 58-59 0.6916215136797845 2307 60-61 0.7912724044458983 2307 62-63 0.7954838580619636 2307 64-65 0.7569019013753362 2307 66-67 0.6926257767373016 2307 68-69 0.67989413924019 2307 70-71 0.6821058085946987 2307 72-73 0.7126184413738841 2307 74-75 0.6652338980174477 2307 76-77 0.6864090104046952 2307 78-79 0.6925061979121736 2307 80-81 0.67466666797133 2307 82-83 0.7035482485558475 2307 84-85 0.712747312138255 2307 86-87 0.7059809461680615 2307 88-89 0.6349264135958137 2307 90-91 0.6569487528691198 2307 92-93 0.6570147854473376 2307 94-95 0.6968337407799226 2307 96-97 0.7447287944659067 2307 98-99 0.7012579072084293 2307 100-101 0.6400796028168081 2201 1 0.3255705384507763 2201 2 0.7059428622521686 2201 3 0.6885012583743553 2201 4 0.7139903137649171 2201 5 0.5750173693038718 2201 6 0.8657933235857698 2201 7 0.6265272192194402 2201 8 0.8310243659998306 2201 9 0.9698783386837277 2201 10-11 0.7634583498487402 2201 12-13 0.5503918812826782 2201 14-15 0.8665723764864524 2201 16-17 0.8165997275997441 2201 18-19 0.8095986823709893 2201 20-21 0.8913306514763306 2201 22-23 0.9637785943706163 2201 24-25 0.8135081151261474 2201 26-27 0.7775399200751494 2201 28-29 0.8708854660115364 2201 30-31 0.8733408140413061 2201 32-33 0.8326273017289907 2201 34-35 0.9221068793131408 2201 36-37 0.809748674622881 2201 38-39 0.8182940418250411 2201 40-41 0.7875651869754137 2201 42-43 0.7960804949007994 2201 44-45 0.7718798151372184 2201 46-47 0.7895589534478304 2201 48-49 0.8028148114807081 2201 50-51 0.5153947322176862 2201 52-53 0.6560325363317432 2201 54-55 0.8053233485436309 2201 56-57 0.8176670948165494 2201 58-59 0.8298758238493491 2201 60-61 0.8671983410620712 2201 62-63 0.800771763913616 2201 64-65 0.81661315721405 2201 66-67 0.7863214008953321 2201 68-69 0.8120749170683084 2201 70-71 0.8499755520599948 2201 72-73 0.7933879310655385 2201 74-75 0.8278209631296107 2201 76-77 0.8000618515689268 2201 78-79 0.8185017745427743 2201 80-81 0.8334685035632319 2201 82-83 0.8210711082189519 2201 84-85 0.7813048570210555 2201 86-87 0.7559609378173775 2201 88-89 0.7703555811818958 2201 90-91 0.8106551158554822 2201 92-93 0.793742510487796 2201 94-95 0.7630790324579522 2201 96-97 0.7750474895753214 2201 98-99 0.7817256328482145 2201 100-101 0.6900599702792114 1101 1 0.3291902417228556 1101 2 0.7569673336222316 1101 3 0.7167984767801237 1101 4 0.7455772331970039 1101 5 0.5821996078471443 1101 6 0.8730196014961678 1101 7 0.6054771358288349 1101 8 0.8366595933851215 1101 9 1.0062057629661396 1101 10-11 0.8118663053030062 1101 12-13 0.5792593540417812 1101 14-15 0.9790420990277298 1101 16-17 0.8316750822398475 1101 18-19 0.8037345687399693 1101 20-21 0.9147004529217284 1101 22-23 1.0029129391412042 1101 24-25 0.9456789011655218 1101 26-27 0.8628253035547502 1101 28-29 0.9497350054412905 1101 30-31 0.9587245913852271 1101 32-33 0.8911022412559859 1101 34-35 0.9886371997463215 1101 36-37 0.9347453759236828 1101 38-39 0.8849737126759791 1101 40-41 0.8232746187094193 1101 42-43 0.8821956666360578 1101 44-45 0.8582678683766432 1101 46-47 0.8423871989078506 1101 48-49 0.8482161664489709 1101 50-51 0.774647373949584 1101 52-53 0.8392520550909168 1101 54-55 0.9163508653030377 1101 56-57 0.9309505626561503 1101 58-59 0.9381568080551617 1101 60-61 0.9632673518026635 1101 62-63 0.9377790667988535 1101 64-65 0.967871311648878 1101 66-67 0.8862564114801472 1101 68-69 0.8428788885130984 1101 70-71 0.8674737571517568 1101 72-73 0.8992490437309542 1101 74-75 0.9274285896339407 1101 76-77 0.9384311486000598 1101 78-79 0.9374839594877002 1101 80-81 0.9458478521435509 1101 82-83 0.8860652052172284 1101 84-85 0.9362378505789195 1101 86-87 0.8932694289526637 1101 88-89 0.9420836780082595 1101 90-91 0.9257277168536504 1101 92-93 0.9419210393649919 1101 94-95 0.9509593375035053 1101 96-97 0.9702445536133126 1101 98-99 0.9371875540761607 1101 100-101 0.9896685388189361 2304 1 0.32955252342632946 2304 2 0.6638065915657627 2304 3 0.6276908441843396 2304 4 0.5864932797481757 2304 5 0.4653167096899864 2304 6 0.7583642152840966 2304 7 0.5867212497057963 2304 8 0.7269788378068895 2304 9 0.9209576986791248 2304 10-11 0.6780897567024695 2304 12-13 0.48133000319448627 2304 14-15 0.7749768304396341 2304 16-17 0.6742028462506724 2304 18-19 0.6176117105049457 2304 20-21 0.7294441686626314 2304 22-23 0.8430548009869909 2304 24-25 0.665572057606667 2304 26-27 0.6666179866139359 2304 28-29 0.7423772461293012 2304 30-31 0.6907104522293395 2304 32-33 0.6549359518237381 2304 34-35 0.7231025779876319 2304 36-37 0.666462148244122 2304 38-39 0.6389480591279053 2304 40-41 0.6516420639219973 2304 42-43 0.6179293925986755 2304 44-45 0.6666089694831072 2304 46-47 0.6362976064375871 2304 48-49 0.6801935460504964 2304 50-51 0.609419343081079 2304 52-53 0.6141273989128493 2304 54-55 0.6287477086729751 2304 56-57 0.7210263632593197 2304 58-59 0.7295947926868322 2304 60-61 0.7127523876349002 2304 62-63 0.6486905873409441 2304 64-65 0.6541921526344225 2304 66-67 0.6527490719896747 2304 68-69 0.673307456553772 2304 70-71 0.6629399903505586 2304 72-73 0.7385261002011632 2304 74-75 0.7095854017800463 2304 76-77 0.7277753113949075 2304 78-79 0.7129124330690289 2304 80-81 0.7187924270222723 2304 82-83 0.6966831983944459 2304 84-85 0.7374870652600691 2304 86-87 0.7637458416523373 2304 88-89 0.7417974059243662 2304 90-91 0.7578406556524371 2304 92-93 0.7437653076729234 2304 94-95 0.7217570526326327 2304 96-97 0.7104156978535272 2304 98-99 0.6499615715684257 2304 100-101 0.6638555956388394 2202 1 0.2108800623591307 2202 2 0.6023061869554063 2202 3 0.5728878838895604 2202 4 0.5532905443796778 2202 5 0.3779101066709103 2202 6 0.6657769726781382 2202 7 0.48154839485469836 2202 8 0.6497211752399039 2202 9 0.8293936281280097 2202 10-11 0.6495518835091971 2202 12-13 0.4398107727214864 2202 14-15 0.7362962713912609 2202 16-17 0.644529128551838 2202 18-19 0.5697671027810784 2202 20-21 0.7389309704215847 2202 22-23 0.7776917084337853 2202 24-25 0.6195535653637734 2202 26-27 0.5865488383632211 2202 28-29 0.6994192974985438 2202 30-31 0.6909727885454728 2202 32-33 0.6118913276354121 2202 34-35 0.7457965149858836 2202 36-37 0.6399487806567166 2202 38-39 0.6011895025329963 2202 40-41 0.5932986769375077 2202 42-43 0.5593986883737401 2202 44-45 0.5540970276468613 2202 46-47 0.5875226866992449 2202 48-49 0.5714710908945477 2202 50-51 0.43734217574825607 2202 52-53 0.47782675732482716 2202 54-55 0.6414421422445642 2202 56-57 0.6485160315745233 2202 58-59 0.6863890337475809 2202 60-61 0.6732479385501406 2202 62-63 0.6418817862465573 2202 64-65 0.677857052403219 2202 66-67 0.6751158820171526 2202 68-69 0.6403988584048648 2202 70-71 0.6366376447818212 2202 72-73 0.6355172544522887 2202 74-75 0.6662526145124161 2202 76-77 0.723051573933148 2202 78-79 0.6567065891472694 2202 80-81 0.6538605077846498 2202 82-83 0.6567213656651347 2202 84-85 0.6717173331665478 2202 86-87 0.6988635879979341 2202 88-89 0.715527807800477 2202 90-91 0.7013159602949273 2202 92-93 0.661874060040013 2202 94-95 0.6876092359045742 2202 96-97 0.611993862797668 2202 98-99 0.6186732656417888 2202 100-101 0.5769124277363993 1102 1 0.21265911355077805 1102 2 0.5646910353150751 1102 3 0.5168584461248038 1102 4 0.5223969917839426 1102 5 0.29642701898900015 1102 6 0.6184118272530554 1102 7 0.44405521115304936 1102 8 0.6307383738594083 1102 9 0.7278657766317593 1102 10-11 0.5813640693309239 1102 12-13 0.3582027272348043 1102 14-15 0.6454918263470191 1102 16-17 0.5433916537039565 1102 18-19 0.4596827598915283 1102 20-21 0.5986365413458898 1102 22-23 0.7016251533513227 1102 24-25 0.563042213339493 1102 26-27 0.44176405998299373 1102 28-29 0.5553071975492472 1102 30-31 0.5399012637961178 1102 32-33 0.49231027850890285 1102 34-35 0.6276139691578777 1102 36-37 0.5043085522189799 1102 38-39 0.500206363602878 1102 40-41 0.5443567592165266 1102 42-43 0.5136617373026908 1102 44-45 0.5407363601719339 1102 46-47 0.411570779942771 1102 48-49 0.530813150097849 1102 50-51 0.4594408198931035 1102 52-53 0.4717897011629191 1102 54-55 0.5321141525950779 1102 56-57 0.5432846628584187 1102 58-59 0.5737241695692319 1102 60-61 0.6026582693141975 1102 62-63 0.6393251970266149 1102 64-65 0.5449514557696986 1102 66-67 0.6087388896428507 1102 68-69 0.6136031568530811 1102 70-71 0.5993699645037793 1102 72-73 0.6355789489949402 1102 74-75 0.6043128371353035 1102 76-77 0.6092987310384785 1102 78-79 0.6208496101745311 1102 80-81 0.6263064961365572 1102 82-83 0.5958290309698313 1102 84-85 0.6292703749578621 1102 86-87 0.5933217684860637 1102 88-89 0.6298115882466888 1102 90-91 0.6283150004367215 1102 92-93 0.5755350719972583 1102 94-95 0.6246526715755252 1102 96-97 0.5386952353879764 1102 98-99 0.5323698871266558 1102 100-101 0.6314690673656038 2305 1 0.27225518606695687 2305 2 0.5183037517812501 2305 3 0.5031860457614492 2305 4 0.4768729966073266 2305 5 0.2942078868813738 2305 6 0.6336386799291347 2305 7 0.36474880612301064 2305 8 0.6116173877326574 2305 9 0.7741463301395228 2305 10-11 0.5158833923944073 2305 12-13 0.33499963550784173 2305 14-15 0.509550977510635 2305 16-17 0.48774132080325927 2305 18-19 0.4504353227933322 2305 20-21 0.5447619785686655 2305 22-23 0.5779418545774533 2305 24-25 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68-69 -1.3060251779873084 1213 70-71 -1.246517461210182 1213 72-73 -1.163534544857761 1213 74-75 -1.3373784854461022 1213 76-77 -1.3306759635614895 1213 78-79 -1.3030226893887118 1213 80-81 -1.177899537231447 1213 82-83 -1.0812854208295377 1213 84-85 -1.1908987170961964 1213 86-87 -1.1506179223359254 1213 88-89 -1.2450851709518886 1213 90-91 -1.2034035692218765 1213 92-93 -1.302466123821496 1213 94-95 -1.1120121709121165 1213 96-97 -1.2699850339798573 1213 98-99 -1.2159639704701988 1213 100-101 -1.0700225077348868 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20288.0 3 1595.0 4 1046.0 5 817.0 6 816.0 7 1351.0 8 2276.0 9 4294.0 10 9011.0 11 15860.0 12 23527.0 13 30241.0 14 36150.0 15 42928.0 16 50565.0 17 57924.0 18 66162.0 19 74525.0 20 84327.0 21 94666.0 22 105542.0 23 119078.0 24 133321.0 25 151123.0 26 174512.0 27 203884.0 28 242847.0 29 291725.0 30 358441.0 31 450357.0 32 562834.0 33 754707.0 34 1061574.0 35 1597678.0 36 3134588.0 37 7814916.0 38 7484591.0 39 6854.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.60627129360047 13.346617699230803 12.626776572530538 53.420334434638185 2 22.5819593052095 20.58008398859973 38.46592903367934 18.372027672511436 3 23.184982262203942 26.016704965374238 24.907950545389525 25.8903622270323 4 25.811376087042763 30.537518018216716 18.84924138110882 24.801864513631703 5 27.311062956383385 31.77260279597408 21.821799199752874 19.094535047889664 6 19.529065598353636 34.50726172989175 22.596570020155262 23.36710265159935 7 19.00022875295968 14.629066678021793 41.826189342092626 24.544515226925906 8 20.908153591663567 20.636132024208166 27.2262344988569 31.229479885271367 9 21.24900046310605 20.02916827239019 31.204647431053 27.51718383345076 10-11 24.845775744583555 28.71501291287815 21.187576821995176 25.251634520543114 12-13 22.595004182641034 22.88007738801965 28.081007066271713 26.443911363067603 14-15 23.48329017009506 24.426654097119567 26.583983852966416 25.506071879818965 16-17 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25.260171768950805 25.409246798004453 24.893514226441475 42-43 24.045716160510583 25.21763111761082 25.674943295124592 25.061709426754003 44-45 24.352796692101588 25.393002337581066 25.560521485294046 24.6936794850233 46-47 24.511178125158427 25.234119653303576 25.31087100085684 24.94383122068116 48-49 24.236102538928016 25.238437502502286 25.412957240246204 25.112502718323494 50-51 24.35372795552227 25.40587383538356 25.504756784008293 24.735641425085877 52-53 24.562800636646624 25.227750278504175 25.28537838913763 24.924070695711574 54-55 24.172696246707247 25.233863775806427 25.47467790300867 25.118762074477658 56-57 24.333969491694567 25.416476003045812 25.597153904599146 24.652400600660478 58-59 24.491910173863868 25.311178357488856 25.267191575075245 24.929719893572035 60-61 24.19447723365147 25.340072946472496 25.43628616577347 25.029163654102565 62-63 24.392181322239466 25.435045751566932 25.48904168536395 24.683731240829648 64-65 24.455903380511575 25.36788997757694 25.304244259333476 24.87196238257801 66-67 24.276424822785415 25.338893225697202 25.39782592421873 24.986856027298657 68-69 24.46305970961557 25.468379506653545 25.437248198356748 24.631312585374136 70-71 24.565738981923406 25.33234219647198 25.253175773525598 24.84874304807902 72-73 24.330151124510273 25.337770683574846 25.38126817422309 24.950810017691794 74-75 24.4531732059216 25.51107399405676 25.429907081959733 24.605845718061907 76-77 24.569604260552172 25.41601850862082 25.237715871786932 24.776661359040077 78-79 24.36798066513272 25.368352504748675 25.37297622196443 24.890690608154177 80-81 24.533452050531633 25.55049476980113 25.30561974413522 24.610433435532016 82-83 24.667467858877863 25.417099862214698 25.155922664323576 24.759509614583862 84-85 24.36832204190401 25.45089629325329 25.34622472656715 24.83455693827555 86-87 24.609974818731793 25.60727285232975 25.334986225502387 24.447766103436074 88-89 24.673872020560054 25.473790072565418 25.095814653952765 24.756523252921763 90-91 24.56955551609159 25.385312526472752 25.189343825993394 24.855788131442264 92-93 24.652945764577485 25.62182737698031 25.2242773990311 24.500949459411107 94-95 24.852924309825326 25.504488584264134 25.04781358930053 24.59477351661001 96-97 24.617321047273073 25.550236305941915 25.136255234186518 24.696187412598494 98-99 24.824203996038715 25.682354828492144 25.128934170141466 24.364507005327674 100-101 25.136728492481964 25.498866574255945 24.80127812648353 24.56312680677856 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18096.0 1 9614.0 2 915.5 3 599.0 4 514.0 5 497.0 6 463.5 7 491.5 8 507.0 9 492.5 10 514.0 11 549.0 12 618.5 13 692.5 14 766.5 15 902.5 16 1074.0 17 1280.5 18 1566.5 19 1993.5 20 2674.5 21 3700.5 22 5195.0 23 7692.0 24 11690.0 25 17440.5 26 25802.5 27 37487.5 28 52498.0 29 71833.0 30 96447.0 31 126477.5 32 162018.0 33 205402.0 34 258613.0 35 323126.0 36 400974.5 37 493558.0 38 600930.5 39 713936.5 40 824802.5 41 930364.5 42 1021479.0 43 1082828.5 44 1110101.5 45 1104249.5 46 1072830.5 47 1022970.5 48 962476.0 49 900062.0 50 836536.0 51 776211.5 52 726419.0 53 693998.5 54 673112.0 55 654576.0 56 638342.5 57 626416.5 58 605536.5 59 571931.0 60 539507.0 61 508391.5 62 481137.0 63 451188.0 64 404369.0 65 362156.5 66 334412.0 67 304514.5 68 271398.0 69 234175.0 70 196548.5 71 164278.5 72 135746.5 73 109738.5 74 83267.0 75 61131.0 76 44555.0 77 31224.5 78 21206.0 79 14211.0 80 9440.0 81 6058.0 82 3943.5 83 2495.0 84 1565.0 85 1090.0 86 805.0 87 592.5 88 450.0 89 356.0 90 296.5 91 238.0 92 174.0 93 136.5 94 117.5 95 81.0 96 45.5 97 31.5 98 17.0 99 6.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15550358866156375 2 0.12982180945449628 3 0.12826245963055044 4 0.13787976945843977 5 0.14340873317430866 6 0.09657678782722452 7 0.08456108715336771 8 0.09388552417168347 9 0.10488804323404247 10-11 0.0785947930934734 12-13 0.08993372011277502 14-15 0.12405933903910252 16-17 0.12321040366540612 18-19 0.1229828335768861 20-21 0.14705183346096387 22-23 0.12502107002189147 24-25 0.11200801869921649 26-27 0.11484967650021426 28-29 0.10350085512923785 30-31 0.08284540657296029 32-33 0.10234915259429307 34-35 0.11967020463616866 36-37 0.11720255332847772 38-39 0.11115116784418026 40-41 0.15451217462375044 42-43 0.11838987552945171 44-45 0.11686218763086516 46-47 0.15066327182225978 48-49 0.1582898380931827 50-51 0.12886403621237727 52-53 0.09519157859275486 54-55 0.09345413043866292 56-57 0.09918296005836244 58-59 0.10000221237703448 60-61 0.0951757476300752 62-63 0.08517651582753924 64-65 0.08597597944286173 66-67 0.10402723463833632 68-69 0.10080563373302688 70-71 0.0971526390946969 72-73 0.10905158641879127 74-75 0.14821145147724846 76-77 0.1634170911310554 78-79 0.15166853795241775 80-81 0.12514178111232382 82-83 0.10378383358713664 84-85 0.10890712888433941 86-87 0.10901596675276205 88-89 0.1050503106015089 90-91 0.11369401622459957 92-93 0.09977068454784456 94-95 0.10165852684739321 96-97 0.09593959157936847 98-99 0.09545081060663417 100-101 0.09149109106638591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.5266941E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.859616667339196 #Duplication Level Percentage of deduplicated Percentage of total 1 75.5098104690409 42.934588778931015 2 13.434384905058325 15.277479517262089 3 4.36193902786801 7.440545431526435 4 2.1813170451280754 4.961154040636616 5 1.3506586952338364 3.839896782970223 6 0.7565771008076346 2.581121035872537 7 0.40733206292765456 1.621252146806805 8 0.34276186229419275 1.5591446478584883 9 0.3022879414912027 1.546917882871396 >10 1.264868547662061 12.487255737325174 >50 0.061047030519168294 2.368545503111098 >100 0.025720726082067328 2.6311713461546367 >500 9.177780030436495E-4 0.34663215804597564 >1k 3.681616106981115E-4 0.37886820900155865 >5k 8.646273110647287E-6 0.025426781626063107 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.003945867447903567 1.9788703349566534E-5 0.0 2 0.004416838587623251 1.9788703349566534E-5 1.9788703349566534E-5 3 0.006506525661337476 2.7704184689393147E-5 2.374644401947984E-5 4 0.013111994839422785 2.7704184689393147E-5 3.1661925359306454E-5 5 0.014251824152357819 3.1661925359306454E-5 3.5619666029219764E-5 6 0.017671312091162916 3.957740669913307E-5 4.353514736904638E-5 7 0.01835995896772783 3.957740669913307E-5 4.353514736904638E-5 8 0.020267589970626044 4.749288803895968E-5 4.353514736904638E-5 9 0.02257099504051559 6.332385071861291E-5 4.749288803895968E-5 10-11 0.02808017005303491 7.123933205843952E-5 4.749288803895968E-5 12-13 0.03389013335646765 1.0092238708278933E-4 4.947175837391633E-5 14-15 0.04036895483311573 1.1081673875757259E-4 5.5408369378786295E-5 16-17 0.04696452965952626 1.2862657177218246E-4 6.530272105356956E-5 18-19 0.05369664653904879 1.3060544210713912E-4 7.321820239339618E-5 20-21 0.05935027908601995 1.5435188612661897E-4 7.915481339826614E-5 22-23 0.06436869425547002 1.8007720048105548E-4 9.102803540800606E-5 24-25 0.0691674548177399 1.99865903830622E-4 1.0092238708278933E-4 26-27 0.0731766461163621 2.1371799617531857E-4 1.1279560909252924E-4 28-29 0.07681380979201241 2.2559121818505848E-4 1.1873222009739921E-4 30-31 0.07967129855568983 2.4735879186958167E-4 1.3060544210713912E-4 32-33 0.08243775928395923 2.671474952191482E-4 1.3456318277705242E-4 34-35 0.0851963045308888 2.730841062240182E-4 1.4445753445183572E-4 36-37 0.08771738533762358 2.8891506890367145E-4 1.5237301579166233E-4 38-39 0.09032949417976635 3.0078829091341133E-4 1.6226736746644557E-4 40-41 0.09262300489798111 3.0870377225323794E-4 1.8007720048105548E-4 42-43 0.0947423750267197 3.4234456794750105E-4 2.45379921534625E-4 44-45 0.09690725917316229 3.680698823019375E-4 2.631897545492349E-4 46-47 0.09899100963587162 3.7202762297185084E-4 2.6516862488419157E-4 48-49 0.10088676741676011 3.8192197464663414E-4 2.730841062240182E-4 50-51 0.10285178565937206 3.918163263214174E-4 2.809995875638448E-4 52-53 0.10479899406896942 4.0171067799620063E-4 2.988094205784547E-4 54-55 0.10667694201684327 4.333726033555071E-4 3.185981239280212E-4 56-57 0.1086617489628048 4.472246957002037E-4 3.2453473493289115E-4 58-59 0.11080882327623276 4.6899226938472687E-4 3.4630230861741433E-4 60-61 0.11317357332650596 4.7295001005464015E-4 3.5619666029219763E-4 62-63 0.11610625916291173 4.947175837391633E-4 3.5817553062715427E-4 64-65 0.1193852473079349 5.0263306507899E-4 3.6213327129706754E-4 66-67 0.12297491809554628 5.065908057489033E-4 3.779642339767208E-4 68-69 0.12734624266546551 5.125274167537732E-4 3.7994310431167745E-4 70-71 0.13328879028134036 5.323161201033397E-4 3.8785858565150406E-4 72-73 0.14147537685705602 5.422104717781231E-4 3.8785858565150406E-4 74-75 0.15251747332611415 5.501259531179496E-4 3.9577406699133066E-4 76-77 0.1671096631760845 5.817878784772561E-4 4.036895483311573E-4 78-79 0.18626512801846493 5.916822301520394E-4 4.116050296709839E-4 80-81 0.21236246999587327 6.114709335016059E-4 4.254571220156805E-4 82-83 0.2509900189342271 6.154286741715193E-4 4.4920356603516033E-4 84-85 0.30583836800822073 6.213652851763891E-4 4.630556583798569E-4 86-87 0.377453685430302 6.273018961812592E-4 4.709711397196835E-4 88-89 0.4661209285287048 6.431328588609123E-4 4.769077507245535E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 6785 0.0 12.743173 1 CTCGAAT 11560 0.0 11.753419 1 CTCGGAT 12975 0.0 11.27715 1 CTCGACT 12505 0.0 11.131147 1 CGGGAAT 7195 0.0 10.696464 1 CGCAAAT 6270 0.0 10.456048 1 CTGGAAT 23200 0.0 10.402357 1 CGAGAAT 9795 0.0 10.233732 1 CTCGATT 13445 0.0 10.211583 1 CTCGGTT 10925 0.0 10.001437 1 CGCGAAT 2870 0.0 9.93174 1 CTCAGAT 21245 0.0 9.749577 1 TTACTCG 7145 0.0 9.707732 3 CTCGTTT 10965 0.0 9.575019 1 CCCGAAT 5715 0.0 9.559554 1 CCGAAAT 7690 0.0 9.5137205 1 CGGAAAT 8280 0.0 9.466941 1 CGGAGAT 22660 0.0 9.371382 1 CTGGGAT 17180 0.0 9.31886 1 CGGCAAT 9380 0.0 9.268389 1 >>END_MODULE