##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 kx0-2.3420000000ca1a.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 24962007 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12909779249721 33.0 33.0 33.0 33.0 33.0 2 31.645485357006752 33.0 33.0 33.0 27.0 33.0 3 31.70456550228513 33.0 33.0 33.0 27.0 33.0 4 35.60583974677998 37.0 37.0 37.0 33.0 37.0 5 35.79836128561297 37.0 37.0 37.0 33.0 37.0 6 35.385652684097074 37.0 37.0 37.0 33.0 37.0 7 35.73020254340927 37.0 37.0 37.0 33.0 37.0 8 35.47315586443029 37.0 37.0 37.0 33.0 37.0 9 35.26707800378391 37.0 37.0 37.0 33.0 37.0 10-11 35.52807769022739 37.0 37.0 37.0 33.0 37.0 12-13 35.82068206695079 37.0 37.0 37.0 33.0 37.0 14-15 37.827676035825164 40.0 37.0 40.0 33.0 40.0 16-17 37.911117363279324 40.0 37.0 40.0 33.0 40.0 18-19 37.970255917322675 40.0 37.0 40.0 33.0 40.0 20-21 37.760616764509365 40.0 37.0 40.0 33.0 40.0 22-23 37.70953671713977 40.0 37.0 40.0 33.0 40.0 24-25 37.84144550155763 40.0 37.0 40.0 33.0 40.0 26-27 37.85584125907825 40.0 37.0 40.0 33.0 40.0 28-29 37.691644285653794 40.0 37.0 40.0 33.0 40.0 30-31 37.66030539932146 40.0 37.0 40.0 33.0 40.0 32-33 37.680367307805014 40.0 37.0 40.0 33.0 40.0 34-35 37.48958014473756 40.0 37.0 40.0 33.0 40.0 36-37 37.55739256062223 40.0 37.0 40.0 33.0 40.0 38-39 37.532303311989295 40.0 37.0 40.0 33.0 40.0 40-41 37.45944987516428 40.0 37.0 40.0 33.0 40.0 42-43 37.358305043340465 40.0 37.0 40.0 33.0 40.0 44-45 37.258281195097815 40.0 37.0 40.0 33.0 40.0 46-47 37.10124458341831 40.0 37.0 40.0 33.0 40.0 48-49 36.9998781748599 40.0 37.0 40.0 33.0 40.0 50-51 35.59746818434912 38.5 35.0 38.5 30.0 38.5 52-53 35.88983602159874 37.0 35.0 38.5 33.0 40.0 54-55 36.60840686808557 37.0 37.0 40.0 33.0 40.0 56-57 36.567554744296004 37.0 37.0 40.0 33.0 40.0 58-59 36.44565885667767 37.0 37.0 40.0 33.0 40.0 60-61 36.27999377213539 37.0 37.0 40.0 33.0 40.0 62-63 36.11762714031768 37.0 37.0 40.0 33.0 40.0 64-65 35.92772255852664 37.0 37.0 40.0 33.0 40.0 66-67 35.60111446567578 37.0 37.0 40.0 30.0 40.0 68-69 35.32606434650867 37.0 37.0 37.0 27.0 40.0 70-71 35.086396218060514 37.0 33.0 37.0 27.0 40.0 72-73 34.86513660540196 37.0 33.0 37.0 27.0 40.0 74-75 34.64091417008255 37.0 33.0 37.0 27.0 38.5 76-77 34.434026258385394 37.0 33.0 37.0 27.0 37.0 78-79 34.272939471573736 37.0 33.0 37.0 27.0 37.0 80-81 34.14006447878971 37.0 33.0 37.0 27.0 37.0 82-83 34.030563407822136 37.0 33.0 37.0 27.0 37.0 84-85 33.922179073982306 37.0 33.0 37.0 27.0 37.0 86-87 33.8243228599367 37.0 33.0 37.0 27.0 37.0 88-89 33.73878925280327 37.0 33.0 37.0 27.0 37.0 90-91 33.64755075583466 37.0 33.0 37.0 27.0 37.0 92-93 33.571874689402975 37.0 33.0 37.0 27.0 37.0 94-95 33.50052820272024 37.0 33.0 37.0 27.0 37.0 96-97 33.424163489738625 37.0 33.0 37.0 27.0 37.0 98-99 33.3674478378281 37.0 33.0 37.0 27.0 37.0 100-101 31.649525056218437 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.4089420061009932 2306 2 0.7848753374658131 2306 3 0.7138571961782922 2306 4 0.7447245562463536 2306 5 0.5930701268334602 2306 6 0.8800912811644324 2306 7 0.6436603012827433 2306 8 0.8444506228457769 2306 9 0.989534691556905 2306 10-11 0.7942270851096112 2306 12-13 0.5795082914966798 2306 14-15 0.9260972586740266 2306 16-17 0.9087278169562367 2306 18-19 0.8163140535941764 2306 20-21 0.8633718181705703 2306 22-23 0.9726678138536329 2306 24-25 0.914969961247003 2306 26-27 0.854511710720459 2306 28-29 0.9391116532727537 2306 30-31 0.907042600066454 2306 32-33 0.8774363642929046 2306 34-35 1.0150864610971269 2306 36-37 0.9089789830036423 2306 38-39 0.8698500654368289 2306 40-41 0.8409004860428695 2306 42-43 0.8198946871047141 2306 44-45 0.8542043257072862 2306 46-47 0.8805171322504677 2306 48-49 0.8478455037930601 2306 50-51 0.8383957347154407 2306 52-53 0.8479155125313156 2306 54-55 0.8987021423406389 2306 56-57 0.8763763924461969 2306 58-59 0.8472644603861212 2306 60-61 0.8424272759916889 2306 62-63 0.8570610544820951 2306 64-65 0.8303240457077905 2306 66-67 0.8311892163554759 2306 68-69 0.8072328808519345 2306 70-71 0.7771869178754756 2306 72-73 0.8171694693599889 2306 74-75 0.759432684700478 2306 76-77 0.7826607294004333 2306 78-79 0.746923093804476 2306 80-81 0.7319944182250069 2306 82-83 0.7082324913321116 2306 84-85 0.771151471157296 2306 86-87 0.687227748019275 2306 88-89 0.7608086420182261 2306 90-91 0.775672889365886 2306 92-93 0.7629700990732218 2306 94-95 0.7748740528156404 2306 96-97 0.7962009054509238 2306 98-99 0.7118602929942242 2306 100-101 0.6344652430415785 2307 1 0.278343866053234 2307 2 0.6805711014466311 2307 3 0.631639571069524 2307 4 0.6206113599365679 2307 5 0.5052272984188306 2307 6 0.7891708584306585 2307 7 0.5552240091392449 2307 8 0.7542967020330593 2307 9 0.8480826888606501 2307 10-11 0.6980848152473058 2307 12-13 0.4327446802876338 2307 14-15 0.7907005378242786 2307 16-17 0.7144743655480568 2307 18-19 0.61952002888885 2307 20-21 0.7465379697578456 2307 22-23 0.8314322376800902 2307 24-25 0.7016889050125386 2307 26-27 0.5675092125677281 2307 28-29 0.801665262765205 2307 30-31 0.7284155950937787 2307 32-33 0.7061376710713958 2307 34-35 0.7641465024408376 2307 36-37 0.6650856774651004 2307 38-39 0.6897918288658431 2307 40-41 0.6290903112577766 2307 42-43 0.6161224855743868 2307 44-45 0.6220969308206818 2307 46-47 0.5541339327271757 2307 48-49 0.614815814281549 2307 50-51 0.5204615290752841 2307 52-53 0.5897943514800446 2307 54-55 0.6564892984706034 2307 56-57 0.6492420483118551 2307 58-59 0.6255708026805351 2307 60-61 0.6789258710506587 2307 62-63 0.6913132722299267 2307 64-65 0.6058330054075114 2307 66-67 0.5346495919578942 2307 68-69 0.5452137432460447 2307 70-71 0.5923464956477389 2307 72-73 0.5977875006183808 2307 74-75 0.5650414133189869 2307 76-77 0.5668484347043119 2307 78-79 0.6279179804584842 2307 80-81 0.5486983705742787 2307 82-83 0.5999517536449019 2307 84-85 0.5939599930572115 2307 86-87 0.5705488949953761 2307 88-89 0.5257905700494305 2307 90-91 0.5578620365031881 2307 92-93 0.5439283509011403 2307 94-95 0.5213846557039687 2307 96-97 0.5324771499933831 2307 98-99 0.5567415305544117 2307 100-101 0.503942571417344 2201 1 0.295544897455585 2201 2 0.6929749454453784 2201 3 0.6673364760967466 2201 4 0.6811561608961796 2201 5 0.535215612896053 2201 6 0.8220769415258076 2201 7 0.6085227603415717 2201 8 0.8047635919619367 2201 9 0.9086248698418871 2201 10-11 0.727157370427399 2201 12-13 0.505566423973093 2201 14-15 0.8101619363623129 2201 16-17 0.7787598423888724 2201 18-19 0.7578462170637152 2201 20-21 0.8558738211675418 2201 22-23 0.9429514637940883 2201 24-25 0.8124771012651593 2201 26-27 0.7162919824887979 2201 28-29 0.8414025936536405 2201 30-31 0.8466466853194703 2201 32-33 0.7861057481369329 2201 34-35 0.8762877079293645 2201 36-37 0.7899418511318572 2201 38-39 0.8198804731445435 2201 40-41 0.7676860008889008 2201 42-43 0.7630256306048153 2201 44-45 0.7528041310354894 2201 46-47 0.7573322248757464 2201 48-49 0.7847569741782578 2201 50-51 0.44943727570484526 2201 52-53 0.6151742602226378 2201 54-55 0.8078453971850124 2201 56-57 0.8031536879130456 2201 58-59 0.7858579361022535 2201 60-61 0.8206113710534808 2201 62-63 0.7856577365914887 2201 64-65 0.796836206448198 2201 66-67 0.7971118754477757 2201 68-69 0.8037088973899174 2201 70-71 0.8490245026521208 2201 72-73 0.7913399231579845 2201 74-75 0.8173043165937486 2201 76-77 0.8213608409377287 2201 78-79 0.8027362161396141 2201 80-81 0.820309513774113 2201 82-83 0.8223299887872173 2201 84-85 0.80795146780126 2201 86-87 0.7847272370775329 2201 88-89 0.8109927463054731 2201 90-91 0.8240030351322005 2201 92-93 0.8296819347940172 2201 94-95 0.7865386630636024 2201 96-97 0.8136314701295291 2201 98-99 0.8073469621454734 2201 100-101 0.681165912319873 1101 1 0.33860508078075924 1101 2 0.7260570659458025 1101 3 0.6619028512468113 1101 4 0.6859683922678599 1101 5 0.5150942547065895 1101 6 0.8081726473868258 1101 7 0.5885118581160071 1101 8 0.8145104666307788 1101 9 0.9346861000906159 1101 10-11 0.7404946178394525 1101 12-13 0.5214725125997575 1101 14-15 0.9127256941351334 1101 16-17 0.7846458137074137 1101 18-19 0.7513789770350812 1101 20-21 0.8221723232287061 1101 22-23 0.9552532238619875 1101 24-25 0.8975345257414133 1101 26-27 0.7735770372656248 1101 28-29 0.8640872251189293 1101 30-31 0.8561404309883542 1101 32-33 0.7937776969670836 1101 34-35 0.9314720276008757 1101 36-37 0.8334764242801853 1101 38-39 0.8233305359885179 1101 40-41 0.7746329540930219 1101 42-43 0.8024363429838104 1101 44-45 0.8425646977261962 1101 46-47 0.8022767346467603 1101 48-49 0.8104191580235565 1101 50-51 0.6853069297255701 1101 52-53 0.7462027795863477 1101 54-55 0.8408111412905015 1101 56-57 0.8675934325247852 1101 58-59 0.8811490324379534 1101 60-61 0.9383594913723741 1101 62-63 0.9195969157373227 1101 64-65 0.9468456852525051 1101 66-67 0.8539371886124982 1101 68-69 0.7764522526250843 1101 70-71 0.8431339183610476 1101 72-73 0.8361347783882991 1101 74-75 0.8594840282348031 1101 76-77 0.879168988816204 1101 78-79 0.8722767358033892 1101 80-81 0.8615816963541008 1101 82-83 0.787530078242554 1101 84-85 0.8364877097845778 1101 86-87 0.7976578720918113 1101 88-89 0.8641581846073976 1101 90-91 0.8372430612255428 1101 92-93 0.8596810591744983 1101 94-95 0.8921751371314102 1101 96-97 0.9031614331540325 1101 98-99 0.8897072310349117 1101 100-101 0.9064498824133089 2304 1 0.33121223685148493 2304 2 0.6387719925486905 2304 3 0.6055907639344973 2304 4 0.5645857881202119 2304 5 0.4420092214525866 2304 6 0.7455796270354256 2304 7 0.5866663847728901 2304 8 0.7523217094103245 2304 9 0.8983393931837185 2304 10-11 0.6326504465285083 2304 12-13 0.45329186253086107 2304 14-15 0.76111641510591 2304 16-17 0.6431637472601182 2304 18-19 0.6056829073275551 2304 20-21 0.741770617278128 2304 22-23 0.832559873692972 2304 24-25 0.6454773162565814 2304 26-27 0.6612933957238596 2304 28-29 0.7283250203213996 2304 30-31 0.7273910468201379 2304 32-33 0.689410496452453 2304 34-35 0.743919407982851 2304 36-37 0.6809671516921725 2304 38-39 0.6822829624211764 2304 40-41 0.6591976214064488 2304 42-43 0.6018547000173271 2304 44-45 0.6831682298690964 2304 46-47 0.6576881694179448 2304 48-49 0.643709447893194 2304 50-51 0.6168081653722695 2304 52-53 0.5946184057653809 2304 54-55 0.6135274653988176 2304 56-57 0.6904628058905615 2304 58-59 0.7055476407086658 2304 60-61 0.7044776116789748 2304 62-63 0.6581090355675414 2304 64-65 0.6733913435564958 2304 66-67 0.6333092313074431 2304 68-69 0.6612875564333578 2304 70-71 0.6567056949732901 2304 72-73 0.7024549760557193 2304 74-75 0.6825269630536965 2304 76-77 0.6759796896954455 2304 78-79 0.602104876802521 2304 80-81 0.6204729546695518 2304 82-83 0.6466459804097155 2304 84-85 0.6673683533805885 2304 86-87 0.6837834063760582 2304 88-89 0.6586487210363146 2304 90-91 0.6407210250631792 2304 92-93 0.6321588447702027 2304 94-95 0.6397641372642013 2304 96-97 0.6301412722044759 2304 98-99 0.5908222614329972 2304 100-101 0.5897563474978078 2202 1 0.19064481676304723 2202 2 0.5928158702503836 2202 3 0.5729121947180253 2202 4 0.5492268580584465 2202 5 0.36059573895478536 2202 6 0.6078782391021917 2202 7 0.5115456705022083 2202 8 0.6650543210670747 2202 9 0.8171190542686446 2202 10-11 0.6422908947378119 2202 12-13 0.44138446624859284 2202 14-15 0.7307242423180043 2202 16-17 0.6548750716960399 2202 18-19 0.5410162897651389 2202 20-21 0.7158913531851567 2202 22-23 0.8007521009681327 2202 24-25 0.624973663629568 2202 26-27 0.5968938744560717 2202 28-29 0.6989804994343345 2202 30-31 0.7159939098429788 2202 32-33 0.6304046434953605 2202 34-35 0.7444424965964913 2202 36-37 0.6301691595645877 2202 38-39 0.6211344708667568 2202 40-41 0.5754717746383591 2202 42-43 0.6317329672262844 2202 44-45 0.5834323641665264 2202 46-47 0.6093673629777072 2202 48-49 0.6135214547279233 2202 50-51 0.445769300885992 2202 52-53 0.5209415456615929 2202 54-55 0.6521691248691468 2202 56-57 0.6498381204446417 2202 58-59 0.6510355877694849 2202 60-61 0.6755723725009304 2202 62-63 0.6431088880767604 2202 64-65 0.7124038945534963 2202 66-67 0.6641003776700742 2202 68-69 0.6380130203063956 2202 70-71 0.6392826389181963 2202 72-73 0.6227195689894955 2202 74-75 0.6227285111278107 2202 76-77 0.6838108036423449 2202 78-79 0.6328146115801658 2202 80-81 0.6478600133223651 2202 82-83 0.6199580048702487 2202 84-85 0.6598189583055074 2202 86-87 0.6755533621927299 2202 88-89 0.6616228166606035 2202 90-91 0.6893019444749839 2202 92-93 0.6352939620966822 2202 94-95 0.682529795711126 2202 96-97 0.6376172920212397 2202 98-99 0.6274881066553419 2202 100-101 0.5783895426464518 1102 1 0.23055077599197915 1102 2 0.5480353503287247 1102 3 0.48128724280671875 1102 4 0.48953029164399453 1102 5 0.2837897885426841 1102 6 0.6168031856546747 1102 7 0.44103561640668687 1102 8 0.651695538330813 1102 9 0.7483004373666304 1102 10-11 0.6018137143958953 1102 12-13 0.37825212079853543 1102 14-15 0.6311367864141104 1102 16-17 0.5538112526819674 1102 18-19 0.4581363971325132 1102 20-21 0.6072817864617477 1102 22-23 0.685247095109176 1102 24-25 0.5530704878113326 1102 26-27 0.4623110061868658 1102 28-29 0.5872200680878379 1102 30-31 0.5782573510878066 1102 32-33 0.5078656509401114 1102 34-35 0.616240516139591 1102 36-37 0.5142573682226086 1102 38-39 0.4879667876115903 1102 40-41 0.511588153359952 1102 42-43 0.4940845607128068 1102 44-45 0.49184529418558043 1102 46-47 0.38585856295745913 1102 48-49 0.5140902940422123 1102 50-51 0.38951988232964396 1102 52-53 0.4442667295076532 1102 54-55 0.4905874237294654 1102 56-57 0.5014319074465661 1102 58-59 0.5360136416069494 1102 60-61 0.5538279623518534 1102 62-63 0.5700515743549914 1102 64-65 0.4759417520133127 1102 66-67 0.5552171586986745 1102 68-69 0.5499631266880911 1102 70-71 0.551588774202969 1102 72-73 0.5940281532124487 1102 74-75 0.5630922901891822 1102 76-77 0.5971769127492195 1102 78-79 0.5766510985669058 1102 80-81 0.580049869350411 1102 82-83 0.5637984095305058 1102 84-85 0.56717446741267 1102 86-87 0.5257580449222914 1102 88-89 0.5588591711726352 1102 90-91 0.5822867493080182 1102 92-93 0.5540104709625737 1102 94-95 0.5810444506233452 1102 96-97 0.5125275717353119 1102 98-99 0.5120907622723578 1102 100-101 0.5839685861355015 2305 1 0.2609919064937998 2305 2 0.514678801456057 2305 3 0.5141647102120182 2305 4 0.44983007668609076 2305 5 0.2379132085191955 2305 6 0.5902932194549066 2305 7 0.35563161087486606 2305 8 0.599820943949652 2305 9 0.7280929568320644 2305 10-11 0.5046729846922844 2305 12-13 0.32481364049020556 2305 14-15 0.4839305736522306 2305 16-17 0.4810113384706014 2305 18-19 0.4375163264577182 2305 20-21 0.528393352408763 2305 22-23 0.5614361716455036 2305 24-25 0.45829973619130726 2305 26-27 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66-67 -1.4041163755955708 1213 68-69 -1.2716094057958642 1213 70-71 -1.1723601142279065 1213 72-73 -1.1023355885701989 1213 74-75 -1.304620709235472 1213 76-77 -1.2689566647265025 1213 78-79 -1.2294954028269345 1213 80-81 -1.097629758603098 1213 82-83 -1.0069558380041599 1213 84-85 -1.1071936200107686 1213 86-87 -1.1139757127880685 1213 88-89 -1.1742045907017342 1213 90-91 -1.1491772748782978 1213 92-93 -1.2280700053537927 1213 94-95 -1.0455002153417254 1213 96-97 -1.1776248333674246 1213 98-99 -1.092932011840226 1213 100-101 -0.9798047409080333 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 20690.0 3 1643.0 4 1040.0 5 760.0 6 770.0 7 1244.0 8 2696.0 9 10796.0 10 42194.0 11 54727.0 12 36795.0 13 33352.0 14 34967.0 15 39778.0 16 46231.0 17 53861.0 18 62047.0 19 71126.0 20 80455.0 21 91877.0 22 104927.0 23 119248.0 24 137838.0 25 165279.0 26 209012.0 27 287748.0 28 275365.0 29 277220.0 30 336637.0 31 424799.0 32 532177.0 33 719939.0 34 1018052.0 35 1540068.0 36 3016765.0 37 7571519.0 38 7530191.0 39 8174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.815862710711198 13.491186194802147 13.941380986034089 49.75157010845257 2 24.165939625927262 18.481266618157616 36.585020282460995 20.76777347345413 3 24.65101972467472 24.496758430929663 25.03358019178446 25.818641652611156 4 26.82951800041578 27.625844766788642 19.89409589764422 25.650541335151356 5 28.274886075429333 29.150940989030243 21.98229475122109 20.59187818431933 6 21.92652569346276 32.0998578997034 22.80341236402506 23.170204042808777 7 20.255740746688307 14.597296996144255 42.39654013710166 22.750422120065778 8 21.282153464060173 21.446790395856848 27.482662536514695 29.788393603568288 9 21.48779593778098 21.103707458630673 30.902481397738292 26.50601520585006 10-11 25.56113755771318 28.793057669228933 21.45026410016852 24.19554067288936 12-13 23.438071897993208 22.56656752297857 28.273784701226457 25.721575877801765 14-15 24.11073767691556 24.157361352276315 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24.758154932165468 40-41 25.025017203088733 24.859357978722336 25.137721007027686 24.97790381116124 42-43 24.730412176210233 24.92536228082377 25.923003282078234 24.421222260887756 44-45 25.58520400303174 25.041539650547527 25.295105290134206 24.078151056286522 46-47 24.492585411268358 24.86911454807155 25.58306145692161 25.055238583738483 48-49 24.53197870724435 24.881148778542748 25.181901418514574 25.404971095698325 50-51 24.798140425929894 25.005841053662902 25.31770287630551 24.878315644101697 52-53 24.294972346262337 25.494208580756922 25.81235799246412 24.39846108051662 54-55 23.81404492058285 25.055107091744024 25.95813138193114 25.17271660574199 56-57 23.970832030028152 25.59004836264768 26.271793267726956 24.16732633959722 58-59 24.15899917893138 25.66354040080075 25.8150979298119 24.362362490455972 60-61 23.837313970686434 26.48439040879226 25.258096032899118 24.420199587622186 62-63 24.049512096940155 26.668905769682503 25.254565984870958 24.02701614850638 64-65 24.019797337753985 26.616656212877032 25.1205467026577 24.242999746711284 66-67 23.910570480625285 26.544830037505296 25.153882552496725 24.390716929372697 68-69 24.155543561442357 26.465406445308844 25.22588850065088 24.15316149259792 70-71 24.29226456491892 26.22467770457438 25.07837884376338 24.404678886743323 72-73 24.077341348047295 26.213763538666264 25.23149986323972 24.477395250046722 74-75 24.26255823281265 26.20964747384952 25.245520366925344 24.28227392641248 76-77 24.3593258344457 26.056029583575185 25.147104291492354 24.43754029048676 78-79 24.192540460745832 26.01118562559803 25.24839091078045 24.54788300287569 80-81 24.359397672550628 26.088728178419775 25.152582765311077 24.399291383718516 82-83 24.465969066055766 25.992806979997752 25.03634437971346 24.50487957423302 84-85 24.198630558096365 26.035318140029844 25.228539439103052 24.53751186277074 86-87 24.43669170517547 26.135447859692373 25.232841528112914 24.19501890701924 88-89 24.53328036905488 25.88147725420187 24.981492914891472 24.603749461851784 90-91 24.434692143346716 25.863558133726738 25.07206035522756 24.629689367698983 92-93 24.52784063820878 26.035926212573486 25.132656891578627 24.303576257639104 94-95 24.66082417597291 25.951001158064763 24.965458069202967 24.422716596759365 96-97 24.393421956130293 25.983761307081828 25.106194353315754 24.51662238347213 98-99 24.669760930709284 26.02659684956896 25.038680639710254 24.2649615800115 100-101 24.97616785911077 25.90895706156655 24.717489359969353 24.397385719353327 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 18531.0 1 9835.5 2 900.5 3 598.0 4 532.5 5 502.0 6 495.0 7 491.5 8 480.5 9 487.0 10 526.0 11 590.5 12 623.0 13 695.0 14 809.5 15 954.0 16 1150.0 17 1424.5 18 1815.0 19 2318.0 20 3079.5 21 4307.0 22 6199.0 23 9036.0 24 13182.5 25 19372.5 26 28086.0 27 39741.5 28 55145.0 29 74625.5 30 98472.0 31 127915.0 32 163465.0 33 207125.0 34 261969.0 35 329503.5 36 410536.5 37 505398.0 38 614136.5 39 727543.0 40 837833.0 41 940510.0 42 1030887.5 43 1087786.5 44 1106675.5 45 1094417.0 46 1056492.5 47 1001491.0 48 938350.0 49 879075.5 50 820485.0 51 766613.0 52 720272.5 53 685501.5 54 664112.0 55 644497.5 56 630184.0 57 608330.5 58 571623.5 59 547353.0 60 524104.5 61 487744.5 62 460808.5 63 431711.0 64 383483.0 65 343607.0 66 317058.5 67 293335.0 68 269787.0 69 232982.5 70 193663.5 71 159980.5 72 128602.5 73 104145.5 74 79709.5 75 57684.5 76 41777.5 77 29449.5 78 19636.0 79 12869.5 80 8323.5 81 5188.0 82 3196.0 83 1939.5 84 1261.0 85 934.5 86 725.0 87 552.0 88 423.0 89 331.0 90 280.0 91 231.5 92 188.5 93 147.0 94 106.5 95 79.5 96 55.0 97 32.5 98 19.5 99 13.5 100 11.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1589776014404611 2 0.13350689309557523 3 0.13170415343605985 4 0.14143494150931055 5 0.14719569624349516 6 0.09891432207354162 7 0.08744489175089167 8 0.09655874225177487 9 0.10763156984933142 10-11 0.08108122075280244 12-13 0.09261675152963461 14-15 0.12635201969136536 16-17 0.12582121301384142 18-19 0.1254446407294093 20-21 0.14935698079084747 22-23 0.12749575785312453 24-25 0.11443591054196883 26-27 0.1178310702340561 28-29 0.10616734463699173 30-31 0.08569423123709564 32-33 0.10472314986531331 34-35 0.12199539884753657 36-37 0.11973596514094399 38-39 0.11389508864411424 40-41 0.15686639299476202 42-43 0.12115612338382886 44-45 0.11939745069376835 46-47 0.15345320590607958 48-49 0.16103472769637472 50-51 0.1316300408056131 52-53 0.0981892201216032 54-55 0.09603995383864766 56-57 0.10178268117623716 58-59 0.102561865317961 60-61 0.09790278482014686 62-63 0.08797569842841563 64-65 0.08848647466527831 66-67 0.10653790778922545 68-69 0.10295646499898826 70-71 0.09932294306303174 72-73 0.11163765798158777 74-75 0.1510295225860645 76-77 0.16594018261432264 78-79 0.1545989471119049 80-81 0.12824489633385647 82-83 0.10649584386383676 84-85 0.11180190759501028 86-87 0.11169374321543936 88-89 0.10801214822189578 90-91 0.11652909159107279 92-93 0.1025177983485062 94-95 0.1041963492759216 96-97 0.09852372848064661 98-99 0.09801695833191618 100-101 0.0942292020028678 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.4962007E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.535815313383395 #Duplication Level Percentage of deduplicated Percentage of total 1 73.98646823255845 37.389664942900815 2 13.158276611373518 13.299284733495686 3 5.138948023540058 7.791027846680909 4 2.4092748150337546 4.87018668366927 5 1.3616148101407186 3.440515728661947 6 0.8460635386584848 2.5653906439819676 7 0.5649092586801641 1.9983704975836767 8 0.41198017642159696 1.6655803286733544 9 0.282159179184463 1.2833229751417687 >10 1.6771564109342885 14.64027079118105 >50 0.10387563292770102 3.6152090106850916 >100 0.05590530548010591 5.071275713032262 >500 0.0027595842479343487 0.9371663671447351 >1k 5.724369452963704E-4 0.48698893740160715 >5k 2.8027869821597535E-5 0.08449338643138633 >10k+ 7.956003848531785E-6 0.8612514133345489 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 214208 0.8581361266343688 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.9599748930444575 4.006088132256353E-6 0.0 2 0.9602833618306412 8.012176264512706E-6 1.2018264396769058E-5 3 0.9607961411115701 1.6024352529025412E-5 1.2018264396769058E-5 4 0.9630996417876175 1.6024352529025412E-5 1.2018264396769058E-5 5 0.9635162749533721 1.6024352529025412E-5 1.2018264396769058E-5 6 0.9647982231556942 5.2079145719332584E-5 2.8042616925794467E-5 7 0.9651868137045231 5.6085233851588934E-5 3.2048705058050824E-5 8 0.966404664496729 6.8103498248358E-5 4.807305758707623E-5 9 0.967390162177264 6.8103498248358E-5 6.009132198384529E-5 10-11 0.9693831910230615 7.411263044674252E-5 6.209436604997347E-5 12-13 0.9714443233671075 9.814915924028064E-5 6.610045418222982E-5 14-15 0.9736596901042452 1.0816437957092152E-4 7.210958638061435E-5 16-17 0.9758209746515976 1.2418873209994695E-4 8.012176264512705E-5 18-19 0.9775455955925338 1.3821004056284417E-4 8.212480671125523E-5 20-21 0.9810689501048533 1.5623743715799775E-4 8.813393890963975E-5 22-23 0.9824169987613576 1.7626787781927953E-4 9.214002704189612E-5 24-25 0.9834846212486039 1.9229223034830493E-4 9.814915924028064E-5 26-27 0.9845061737223293 1.9229223034830493E-4 1.0816437957092152E-4 28-29 0.9854896683587981 1.9229223034830493E-4 1.2819482023220327E-4 30-31 0.9862808707649189 2.103196269434585E-4 1.5623743715799775E-4 32-33 0.9870660640388411 2.2233789134022756E-4 1.9028918628217676E-4 34-35 0.9878212116517714 2.3035006760474026E-4 2.2233789134022756E-4 36-37 0.9885262831630486 2.4637442013376566E-4 2.3435615573699664E-4 38-39 0.9892593972912515 2.50380508266022E-4 2.4036528793538114E-4 40-41 0.9900185509923141 2.603957285966629E-4 2.603957285966629E-4 42-43 0.9907236225035911 2.6840790486117565E-4 2.784231251918165E-4 44-45 0.9915048096893812 2.7441703705956017E-4 2.804261692579447E-4 46-47 0.9922920060073694 2.8443225739020105E-4 2.8242921332407287E-4 48-49 0.9934097045962691 2.964505217869701E-4 2.884383455224574E-4 50-51 0.9946676162697976 3.024596539853546E-4 3.0045660991922645E-4 52-53 0.9962159693329147 3.0846878618373915E-4 3.06465742117611E-4 54-55 0.9975479936368898 3.124748743159955E-4 3.224900946466364E-4 56-57 0.9990102158051635 3.2449313871276455E-4 3.3250531497727725E-4 58-59 1.0007748976274224 3.2649618277889273E-4 3.365114031095336E-4 60-61 1.0037153663164986 3.445235793740463E-4 3.5854488783694353E-4 62-63 1.0066638471818392 3.7056315223371264E-4 3.7657228443209716E-4 64-65 1.0101851986500927 3.905935928949944E-4 3.805783725643535E-4 66-67 1.0138768088639667 3.966027250933789E-4 3.885905488288662E-4 68-69 1.0186700933142117 4.066179454240198E-4 3.9459968102725074E-4 70-71 1.025083840413954 4.2464534201917337E-4 4.066179454240198E-4 72-73 1.034381970968921 4.286514301514297E-4 4.146301216885325E-4 74-75 1.0466646371824189 4.506849148788397E-4 4.1863620982078884E-4 76-77 1.0604896473268357 4.76724487738506E-4 4.286514301514297E-4 78-79 1.0778259937191748 4.76724487738506E-4 4.446757826804551E-4 80-81 1.101453901523223 4.907457962014031E-4 4.607001352094805E-4 82-83 1.1352592762272682 4.927488402675313E-4 4.8073057587076233E-4 84-85 1.181950233408716 5.10776236862685E-4 5.047671046643004E-4 86-87 1.2388446970630205 5.227945012594541E-4 5.127792809288132E-4 88-89 1.3050713430214165 5.528401622513767E-4 5.268005893917104E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGAGCGT 41610 0.0 56.421375 9 AAGAGCG 45775 0.0 50.347595 8 ATCGGAA 53175 0.0 45.181423 3 TCGGAAG 53385 0.0 44.532036 4 CGGAAGA 54795 0.0 42.354107 5 GATCGGA 58710 0.0 42.31789 2 AGATCGG 59410 0.0 42.189636 1 GCCGTAT 33185 0.0 39.0282 48-49 CCGTATC 34470 0.0 38.772427 48-49 CGTATCA 33395 0.0 38.598465 50-51 CGCCGTA 34715 0.0 38.507828 46-47 GAGTGTA 32895 0.0 37.536533 28-29 TCGTGTA 36210 0.0 35.64564 14-15 GTAGGGA 36035 0.0 35.43747 18-19 TGTAGGG 35000 0.0 35.352024 18-19 AGTGTAG 35820 0.0 34.782936 28-29 CGTGTAG 35745 0.0 34.561634 16-17 GTCGTGT 36345 0.0 34.474113 14-15 GTGTAGA 37220 0.0 33.866653 30-31 AGAGTGT 37425 0.0 33.692654 26-27 >>END_MODULE