FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 kx0-2.3420000000ca1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 kx0-2.3420000000ca1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24962007
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2142080.8581361266343688Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT416100.056.4213759
AAGAGCG457750.050.3475958
ATCGGAA531750.045.1814233
TCGGAAG533850.044.5320364
CGGAAGA547950.042.3541075
GATCGGA587100.042.317892
AGATCGG594100.042.1896361
GCCGTAT331850.039.028248-49
CCGTATC344700.038.77242748-49
CGTATCA333950.038.59846550-51
CGCCGTA347150.038.50782846-47
GAGTGTA328950.037.53653328-29
TCGTGTA362100.035.6456414-15
GTAGGGA360350.035.4374718-19
TGTAGGG350000.035.35202418-19
AGTGTAG358200.034.78293628-29
CGTGTAG357450.034.56163416-17
GTCGTGT363450.034.47411314-15
GTGTAGA372200.033.86665330-31
AGAGTGT374250.033.69265426-27
Produced by FastQC (version 0.11.2)