FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 k48-3.3420000000ccac.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 k48-3.3420000000ccac.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16818790
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT31900.026.2094881
CGCGTGT24950.019.8020863
TAAGTCG41750.019.226419
GCGTATT43200.019.0222852
ACATGCG41750.018.0920944
CATGCGT44800.016.7541895
GCCGTAT45150.015.41532248-49
CCGTATC53500.013.98619448-49
ATGCGTG60100.013.5890026
CGTATTT67950.013.4233213
GTATTTA68250.013.2947114
TCCGTAC40650.013.2057235
TTAAGTC62000.013.1748788
GTCCGTA41350.013.0971724
GAGTGTA50100.013.08181528-29
CGCGCGT53000.012.8173041
TCGTGTA56850.012.66007114-15
AGAGCGT99800.012.6119829
TGCGTGC68300.012.5832097
CGCCGTA61200.012.42359746-47
Produced by FastQC (version 0.11.2)