##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 k48-3.3420000000ccac.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16818790 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.15848631203553 33.0 33.0 33.0 33.0 33.0 2 31.706743826398927 33.0 33.0 33.0 27.0 33.0 3 31.767957801958406 33.0 33.0 33.0 27.0 33.0 4 35.66912031127091 37.0 37.0 37.0 33.0 37.0 5 35.8459598461007 37.0 37.0 37.0 33.0 37.0 6 35.44700421373951 37.0 37.0 37.0 33.0 37.0 7 35.780653305023726 37.0 37.0 37.0 33.0 37.0 8 35.53719857373806 37.0 37.0 37.0 33.0 37.0 9 35.350891889368974 37.0 37.0 37.0 33.0 37.0 10-11 35.59751646818826 37.0 37.0 37.0 33.0 37.0 12-13 35.87455453097399 37.0 37.0 37.0 33.0 37.0 14-15 37.899229611642696 40.0 37.0 40.0 33.0 40.0 16-17 37.97222802591625 40.0 37.0 40.0 33.0 40.0 18-19 38.04243013320221 40.0 37.0 40.0 33.0 40.0 20-21 37.84700430292548 40.0 37.0 40.0 33.0 40.0 22-23 37.73443725737702 40.0 37.0 40.0 33.0 40.0 24-25 37.88206847817233 40.0 37.0 40.0 33.0 40.0 26-27 37.89354451182279 40.0 37.0 40.0 33.0 40.0 28-29 37.739483993795034 40.0 37.0 40.0 33.0 40.0 30-31 37.7267070342159 40.0 37.0 40.0 33.0 40.0 32-33 37.735689398583375 40.0 37.0 40.0 33.0 40.0 34-35 37.5219791376193 40.0 37.0 40.0 33.0 40.0 36-37 37.567420783540314 40.0 37.0 40.0 33.0 40.0 38-39 37.52012695324693 40.0 37.0 40.0 33.0 40.0 40-41 37.43038562821701 40.0 37.0 40.0 33.0 40.0 42-43 37.35206637932931 40.0 37.0 40.0 33.0 40.0 44-45 37.22360642471902 40.0 37.0 40.0 33.0 40.0 46-47 37.109689073946456 40.0 37.0 40.0 33.0 40.0 48-49 36.96467055596746 40.0 37.0 40.0 33.0 40.0 50-51 35.550077145858886 38.5 35.0 38.5 30.0 38.5 52-53 35.89336988570521 37.0 35.0 38.5 33.0 40.0 54-55 36.57363627228832 37.0 37.0 40.0 33.0 40.0 56-57 36.51386939845257 37.0 37.0 40.0 33.0 40.0 58-59 36.384117228409416 37.0 37.0 40.0 33.0 40.0 60-61 36.19025863334996 37.0 37.0 40.0 33.0 40.0 62-63 35.99856470055218 37.0 37.0 40.0 33.0 40.0 64-65 35.83860194461076 37.0 37.0 40.0 33.0 40.0 66-67 35.63528981573585 37.0 37.0 40.0 33.0 40.0 68-69 35.45332996606771 37.0 37.0 37.0 33.0 40.0 70-71 35.238716786403785 37.0 33.0 37.0 27.0 40.0 72-73 35.0168883730637 37.0 33.0 37.0 27.0 40.0 74-75 34.79809656342698 37.0 33.0 37.0 27.0 38.5 76-77 34.59769281856781 37.0 33.0 37.0 27.0 37.0 78-79 34.43638252216717 37.0 33.0 37.0 27.0 37.0 80-81 34.30666228069914 37.0 33.0 37.0 27.0 37.0 82-83 34.20279856636536 37.0 33.0 37.0 27.0 37.0 84-85 34.096518209692846 37.0 33.0 37.0 27.0 37.0 86-87 33.9947960881847 37.0 33.0 37.0 27.0 37.0 88-89 33.90161700098521 37.0 33.0 37.0 27.0 37.0 90-91 33.80623579341915 37.0 33.0 37.0 27.0 37.0 92-93 33.729942255061154 37.0 33.0 37.0 27.0 37.0 94-95 33.65476134133311 37.0 33.0 37.0 27.0 37.0 96-97 33.57551714481244 37.0 33.0 37.0 27.0 37.0 98-99 33.50800313221106 37.0 33.0 37.0 27.0 37.0 100-101 31.751269710841267 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.44331066194637003 2306 2 0.8030135280783917 2306 3 0.7457790491987062 2306 4 0.8003441895075696 2306 5 0.649883457367558 2306 6 0.9265119652204348 2306 7 0.6901736091352433 2306 8 0.902628661799973 2306 9 1.042496809470883 2306 10-11 0.8233683835471695 2306 12-13 0.6269426118971992 2306 14-15 0.9862264172976154 2306 16-17 0.9580438352655349 2306 18-19 0.8639632137951736 2306 20-21 0.9226315468675281 2306 22-23 1.0546966596605571 2306 24-25 0.987778669892549 2306 26-27 0.925516770764439 2306 28-29 1.0148738057875732 2306 30-31 0.9625837689698855 2306 32-33 0.9435082397025027 2306 34-35 1.0327637764296114 2306 36-37 0.9505331755315254 2306 38-39 0.9164953247999321 2306 40-41 0.8578896731526555 2306 42-43 0.8500327233735376 2306 44-45 0.8938499429442217 2306 46-47 0.8913313136976839 2306 48-49 0.8920080524581024 2306 50-51 0.8351738868203284 2306 52-53 0.8063859137142373 2306 54-55 0.9114733843238412 2306 56-57 0.9060294489046541 2306 58-59 0.868296872917341 2306 60-61 0.9012077761375537 2306 62-63 0.9142521379817836 2306 64-65 0.8956109138605015 2306 66-67 0.8754982262579674 2306 68-69 0.8141588221172356 2306 70-71 0.8099609463148312 2306 72-73 0.8464164855844203 2306 74-75 0.8051396874727672 2306 76-77 0.845882573955322 2306 78-79 0.8334432971814252 2306 80-81 0.8283347236054013 2306 82-83 0.8039692555323867 2306 84-85 0.8822359950933389 2306 86-87 0.8086687427421779 2306 88-89 0.8064447502222833 2306 90-91 0.8647345262000883 2306 92-93 0.8789083308828651 2306 94-95 0.85770739020316 2306 96-97 0.8617431363320307 2306 98-99 0.805770553585937 2306 100-101 0.6396574389125078 2307 1 0.30314879860416966 2307 2 0.7101298247772512 2307 3 0.656603550972239 2307 4 0.6762793249502366 2307 5 0.5364918163791401 2307 6 0.8082151565417846 2307 7 0.5771556543909142 2307 8 0.7660216194291039 2307 9 0.9073619348661808 2307 10-11 0.7185216391100582 2307 12-13 0.4570997332261868 2307 14-15 0.7951422556689351 2307 16-17 0.7373704599469662 2307 18-19 0.6408639216001575 2307 20-21 0.7913132740976252 2307 22-23 0.8836890787956264 2307 24-25 0.7107648472135395 2307 26-27 0.603573308239362 2307 28-29 0.8265849105373206 2307 30-31 0.7637291788360017 2307 32-33 0.7217451798379031 2307 34-35 0.7195075892588463 2307 36-37 0.6654418183541821 2307 38-39 0.6369874722421045 2307 40-41 0.616173567928584 2307 42-43 0.635311112542766 2307 44-45 0.6201480836782736 2307 46-47 0.5483226312276059 2307 48-49 0.6041502932989573 2307 50-51 0.5293172132123871 2307 52-53 0.6061931589661782 2307 54-55 0.6836737610450712 2307 56-57 0.6344753230324471 2307 58-59 0.5868893275483131 2307 60-61 0.6369509743480108 2307 62-63 0.649282668537289 2307 64-65 0.5965796803023906 2307 66-67 0.5498151917362222 2307 68-69 0.5436499709537088 2307 70-71 0.5871818502457486 2307 72-73 0.5765364874519108 2307 74-75 0.4754624008704553 2307 76-77 0.5416487136041823 2307 78-79 0.5745978015662203 2307 80-81 0.5546983077392653 2307 82-83 0.551179635272554 2307 84-85 0.5624173255405864 2307 86-87 0.584997116815984 2307 88-89 0.48788490905426585 2307 90-91 0.568210750440052 2307 92-93 0.5206213132167576 2307 94-95 0.49857759898663545 2307 96-97 0.5464284640314858 2307 98-99 0.5845590880911615 2307 100-101 0.5149263323599271 2201 1 0.34525829602799973 2201 2 0.7309508280491563 2201 3 0.7188603914966585 2201 4 0.7315264295244575 2201 5 0.5844751598532127 2201 6 0.8719735868717464 2201 7 0.6304037741877693 2201 8 0.8538761164411497 2201 9 1.0112364715572824 2201 10-11 0.7772991624975987 2201 12-13 0.5579201815164581 2201 14-15 0.8642554739040591 2201 16-17 0.8182341870937293 2201 18-19 0.8032761337022691 2201 20-21 0.9158625893038987 2201 22-23 0.9949390480168887 2201 24-25 0.8127015608435073 2201 26-27 0.8230290586904587 2201 28-29 0.9011697428387535 2201 30-31 0.8891417352475557 2201 32-33 0.8602762376383524 2201 34-35 0.9841591575694935 2201 36-37 0.8826310235517099 2201 38-39 0.8761270989296932 2201 40-41 0.8342538964383408 2201 42-43 0.834633740299914 2201 44-45 0.8175371252307144 2201 46-47 0.8327183994314709 2201 48-49 0.8562675542134457 2201 50-51 0.5165535302579585 2201 52-53 0.67188347277677 2201 54-55 0.8600155997468448 2201 56-57 0.8779442687172647 2201 58-59 0.8931908140836384 2201 60-61 0.9035751285613856 2201 62-63 0.8525460716130979 2201 64-65 0.8762838949129943 2201 66-67 0.898866416580816 2201 68-69 0.9049030097342197 2201 70-71 0.8798270506077515 2201 72-73 0.8629035844692297 2201 74-75 0.9004964643314324 2201 76-77 0.8541801283647743 2201 78-79 0.877504787908336 2201 80-81 0.868906031085551 2201 82-83 0.9200343261157897 2201 84-85 0.8386025548640674 2201 86-87 0.8187026625311375 2201 88-89 0.8789784601876462 2201 90-91 0.8892086647493684 2201 92-93 0.900058763507694 2201 94-95 0.876596839742227 2201 96-97 0.8485272650224047 2201 98-99 0.8202442301433734 2201 100-101 0.7613284364533328 1101 1 0.35268024268928855 1101 2 0.7656391503998528 1101 3 0.6972359095082616 1101 4 0.7342846990335588 1101 5 0.560731742620483 1101 6 0.8760702969976819 1101 7 0.6232288049954704 1101 8 0.8311905478460133 1101 9 0.9769010329631769 1101 10-11 0.8262086727985363 1101 12-13 0.5903701959508041 1101 14-15 0.9658091832165567 1101 16-17 0.8127714621624094 1101 18-19 0.7963171857590652 1101 20-21 0.9090625076521874 1101 22-23 1.017788123763978 1101 24-25 0.9505081318656039 1101 26-27 0.8552207318933185 1101 28-29 0.9173503651821875 1101 30-31 0.9308738238689571 1101 32-33 0.8852932263432294 1101 34-35 0.9788677980394098 1101 36-37 0.9331681244286187 1101 38-39 0.8983758881247397 1101 40-41 0.8455771877500098 1101 42-43 0.8800488747680433 1101 44-45 0.9058997255290393 1101 46-47 0.851642408288086 1101 48-49 0.8975991053648968 1101 50-51 0.7806519635048446 1101 52-53 0.8612593536741571 1101 54-55 0.9646069528791941 1101 56-57 0.9859200370732779 1101 58-59 0.9866629571421726 1101 60-61 0.9897313169975277 1101 62-63 0.9816034373267968 1101 64-65 0.9759437730081189 1101 66-67 0.8927237181278471 1101 68-69 0.8417585878447298 1101 70-71 0.8634474063532664 1101 72-73 0.8987946271365885 1101 74-75 0.8621673235050125 1101 76-77 0.935313688130023 1101 78-79 0.9205455545847911 1101 80-81 0.8967202649811554 1101 82-83 0.8497590729439892 1101 84-85 0.9284362599030018 1101 86-87 0.8967388715602098 1101 88-89 0.9619386290098717 1101 90-91 0.9056230588613374 1101 92-93 0.9840558473597412 1101 94-95 0.9752963684297526 1101 96-97 0.9680337056380282 1101 98-99 0.9608237272870426 1101 100-101 0.9204025788260921 2304 1 0.32666224772633257 2304 2 0.6398079538724879 2304 3 0.6260229087679967 2304 4 0.5608378781890409 2304 5 0.428317748907169 2304 6 0.7339082692560623 2304 7 0.5602640781220742 2304 8 0.7261889852099657 2304 9 0.9032083484529565 2304 10-11 0.654716637953932 2304 12-13 0.4689805861612726 2304 14-15 0.7538419429061562 2304 16-17 0.6596239209987402 2304 18-19 0.600354127594926 2304 20-21 0.7003393866159371 2304 22-23 0.8268288810084243 2304 24-25 0.6826552767383163 2304 26-27 0.6534970470858497 2304 28-29 0.7441422886758815 2304 30-31 0.7292119797026473 2304 32-33 0.6661049357393054 2304 34-35 0.7256337407363205 2304 36-37 0.6611144973996588 2304 38-39 0.6437979154467186 2304 40-41 0.6603095078492913 2304 42-43 0.6071868864148442 2304 44-45 0.652890181577682 2304 46-47 0.6123376700491363 2304 48-49 0.6049697458894769 2304 50-51 0.5787704784406174 2304 52-53 0.5815300575341524 2304 54-55 0.6204719665366127 2304 56-57 0.6990341680688701 2304 58-59 0.6793291720706236 2304 60-61 0.7110305746225549 2304 62-63 0.6240066662795201 2304 64-65 0.6438964745062208 2304 66-67 0.5984277257960287 2304 68-69 0.6274267061742691 2304 70-71 0.5952163078271084 2304 72-73 0.6857351637750853 2304 74-75 0.6929469994666562 2304 76-77 0.7244855962582619 2304 78-79 0.6405022299760006 2304 80-81 0.690463764701569 2304 82-83 0.7010847658725581 2304 84-85 0.723227048150342 2304 86-87 0.6936493217402031 2304 88-89 0.6860668091361575 2304 90-91 0.682089515712434 2304 92-93 0.7049552629301488 2304 94-95 0.6804481636821222 2304 96-97 0.6702209047356149 2304 98-99 0.6370960227523526 2304 100-101 0.6663404432776048 2202 1 0.2605394638088967 2202 2 0.6225639041647959 2202 3 0.5833836289588596 2202 4 0.5899216708098294 2202 5 0.4227252956546508 2202 6 0.6906913394604075 2202 7 0.5414494141086834 2202 8 0.7008325897546257 2202 9 0.8585981135516789 2202 10-11 0.673530944369702 2202 12-13 0.458364075380743 2202 14-15 0.7544740821148892 2202 16-17 0.6858507295630574 2202 18-19 0.6079024872294951 2202 20-21 0.7372250846954174 2202 22-23 0.848574542675081 2202 24-25 0.7128992914242218 2202 26-27 0.6379793146990025 2202 28-29 0.7327413658754054 2202 30-31 0.6929326031947269 2202 32-33 0.6451149643983172 2202 34-35 0.7810410253852424 2202 36-37 0.6499708603933385 2202 38-39 0.617084733119249 2202 40-41 0.6086286546244679 2202 42-43 0.6196390163929166 2202 44-45 0.6367933050384806 2202 46-47 0.6414998722119947 2202 48-49 0.6439048268247092 2202 50-51 0.512145037540968 2202 52-53 0.5488691065803124 2202 54-55 0.6867028952632808 2202 56-57 0.6738101315648706 2202 58-59 0.7187438756903504 2202 60-61 0.689155899933894 2202 62-63 0.7042666621625528 2202 64-65 0.7515962318627984 2202 66-67 0.6954949374214223 2202 68-69 0.7068403687066009 2202 70-71 0.7511686391258081 2202 72-73 0.7056437394106538 2202 74-75 0.7083106710541784 2202 76-77 0.7613725971774841 2202 78-79 0.6825126823403664 2202 80-81 0.7218925777476954 2202 82-83 0.6667160539534009 2202 84-85 0.6991376780088601 2202 86-87 0.7626830436787415 2202 88-89 0.7503210976518275 2202 90-91 0.74439382895563 2202 92-93 0.6855389261142548 2202 94-95 0.6939316416425072 2202 96-97 0.6824259803113435 2202 98-99 0.70437168773406 2202 100-101 0.6289845157148335 1102 1 0.23020871654632202 1102 2 0.594598141679338 1102 3 0.5285917186714322 1102 4 0.5163579847655555 1102 5 0.31810511288976073 1102 6 0.6261856504388277 1102 7 0.4498233949195196 1102 8 0.6391533800894891 1102 9 0.7268437632761078 1102 10-11 0.5839197800310956 1102 12-13 0.34655953279544605 1102 14-15 0.6223780126697562 1102 16-17 0.5365304681623329 1102 18-19 0.46289616313480764 1102 20-21 0.5955554406298091 1102 22-23 0.698199634197934 1102 24-25 0.5512200795017748 1102 26-27 0.4548594556782817 1102 28-29 0.5738924955759614 1102 30-31 0.5507567426180842 1102 32-33 0.5261221681236421 1102 34-35 0.5722938313410921 1102 36-37 0.46503769318034927 1102 38-39 0.4544146094192669 1102 40-41 0.4974536502023881 1102 42-43 0.4645991603301809 1102 44-45 0.46789867715104094 1102 46-47 0.37705911486644084 1102 48-49 0.499167457339297 1102 50-51 0.3100175381398387 1102 52-53 0.39097430189993787 1102 54-55 0.48010259998837057 1102 56-57 0.5106459266811711 1102 58-59 0.5154053130602705 1102 60-61 0.5295088203536835 1102 62-63 0.5722979820518574 1102 64-65 0.511310529779486 1102 66-67 0.5471956191216307 1102 68-69 0.5473022993992487 1102 70-71 0.5328313107957712 1102 72-73 0.5848234454594277 1102 74-75 0.581366838116459 1102 76-77 0.6188419498327136 1102 78-79 0.6106665401419562 1102 80-81 0.6034887308489374 1102 82-83 0.5741883907795184 1102 84-85 0.6199927978212898 1102 86-87 0.6041137486572836 1102 88-89 0.6010259263112019 1102 90-91 0.6221907284517343 1102 92-93 0.569050201359282 1102 94-95 0.6117201466342905 1102 96-97 0.5253025567188061 1102 98-99 0.5360941195705848 1102 100-101 0.6947763778762948 2305 1 0.24822151340675447 2305 2 0.49264705022150324 2305 3 0.5033442367077008 2305 4 0.4219035599263421 2305 5 0.29226893728835535 2305 6 0.6406664460552278 2305 7 0.35896863255298683 2305 8 0.5738987618613649 2305 9 0.7622279483009464 2305 10-11 0.511886927040031 2305 12-13 0.3330303140361437 2305 14-15 0.5026835428210106 2305 16-17 0.5135913605783671 2305 18-19 0.47179563602838925 2305 20-21 0.554582200858512 2305 22-23 0.577515900902327 2305 24-25 0.46358960913016745 2305 26-27 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72-73 -1.1485245469183027 1213 74-75 -1.3622781988960995 1213 76-77 -1.3395908480830627 1213 78-79 -1.279303644281221 1213 80-81 -1.1353162492229387 1213 82-83 -1.0726629507014351 1213 84-85 -1.1472817392338541 1213 86-87 -1.120169070734434 1213 88-89 -1.206964589328443 1213 90-91 -1.227845366107502 1213 92-93 -1.300145581061031 1213 94-95 -1.1143065556254967 1213 96-97 -1.248876453707986 1213 98-99 -1.143806240576815 1213 100-101 -1.0279395573008117 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13861.0 3 1086.0 4 681.0 5 510.0 6 532.0 7 880.0 8 1674.0 9 4417.0 10 11980.0 11 17645.0 12 19373.0 13 22581.0 14 26115.0 15 30317.0 16 35151.0 17 40334.0 18 45853.0 19 51691.0 20 57915.0 21 64853.0 22 71703.0 23 80921.0 24 89772.0 25 102199.0 26 118298.0 27 139047.0 28 161753.0 29 192607.0 30 234750.0 31 295369.0 32 370970.0 33 499641.0 34 704869.0 35 1062672.0 36 2133169.0 37 5301623.0 38 4807362.0 39 4616.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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25.108274599886705 25.51106968411212 24.973447021315963 44-45 24.637417294628637 25.132598851774308 25.393522437452038 24.836461416145024 46-47 24.628741812865307 25.050016486072174 25.25070471482608 25.070536986236437 48-49 24.349192931821463 25.17060147217392 25.253582619005055 25.226622976999565 50-51 24.368298588398783 25.215712976701848 25.517515570431193 24.898472864468175 52-53 24.592282247821366 25.15026403613235 25.294761762505647 24.962691953540638 54-55 24.321794403692028 25.154101859315936 25.361363377341178 25.162740359650854 56-57 24.290477994711736 25.210496130935056 25.63012841412678 24.868897460226428 58-59 24.589303070676944 25.158276832693875 25.22110993420975 25.03131016241943 60-61 24.39252151506914 25.18757736028949 25.349190528117372 25.070710596523995 62-63 24.429037315312048 25.301076909806312 25.419134734366324 24.850751040515316 64-65 24.501916548443585 25.304283289315638 25.14224781646957 25.051552345771206 66-67 24.403046118721704 25.26053184822427 25.17245036745307 25.163971665600954 68-69 24.532113359242437 25.311707681412184 25.26404841360257 24.89213054574281 70-71 24.650314559698998 25.251119106192238 25.06353879460508 25.03502753950368 72-73 24.498904006205457 25.291615956250606 25.26943447398771 24.940045563556225 74-75 24.72825696179968 25.264569125007974 25.242432563652883 24.76474134953946 76-77 24.852499563379112 25.2029695282544 25.056020730298123 24.888510178068366 78-79 24.602986952645225 25.20336089116406 25.194002751465767 24.999649404724952 80-81 24.74767615355135 25.299707914584552 25.078871992171365 24.873743939692734 82-83 24.754441697104525 25.292040937335614 24.954838723501567 24.9986786420583 84-85 24.57192831402117 25.222532455579444 25.102434795315677 25.103104435083708 86-87 24.695056121542613 25.436620181922137 25.129565452871276 24.738758243663973 88-89 24.830682334426587 25.309893297870616 24.976104478715747 24.88331988898705 90-91 24.78780848925573 25.17953246823132 25.063468308763998 24.96919073374895 92-93 24.764720198224087 25.36955280008899 25.20612184881777 24.659605152869155 94-95 24.78486886196253 25.2489230051341 25.030122525649677 24.936085607253688 96-97 24.596370376114717 25.3575824853516 25.05986812437572 24.986179014157965 98-99 24.830541346691344 25.231766707171786 25.13209346103928 24.805598485097594 100-101 24.995517917869094 25.187173981528854 24.938787032673567 24.87852106792849 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12363.0 1 6555.0 2 617.0 3 449.5 4 367.0 5 336.0 6 331.0 7 321.5 8 327.0 9 322.5 10 353.0 11 405.5 12 451.5 13 500.5 14 584.0 15 662.5 16 780.0 17 1030.0 18 1300.5 19 1669.0 20 2174.5 21 2962.5 22 4189.0 23 5889.5 24 8502.0 25 12374.5 26 17536.0 27 24365.0 28 33512.5 29 45699.0 30 60614.0 31 78585.0 32 100569.0 33 127238.5 34 161378.5 35 205111.0 36 257242.0 37 321811.5 38 393412.0 39 463723.5 40 535179.0 41 601044.5 42 654457.5 43 694349.0 44 721497.5 45 721165.5 46 699186.5 47 678459.5 48 642296.5 49 599260.5 50 571505.0 51 536321.5 52 498681.5 53 473559.5 54 456267.0 55 444952.5 56 439020.0 57 433682.0 58 412932.5 59 379316.0 60 355027.0 61 337299.5 62 316003.0 63 291136.0 64 257171.5 65 231918.5 66 222103.5 67 210680.0 68 194415.5 69 170145.0 70 144224.0 71 120664.5 72 97980.0 73 81617.0 74 67364.0 75 52526.0 76 37781.5 77 25410.5 78 17952.5 79 14924.0 80 11581.0 81 7508.5 82 3985.5 83 1703.0 84 1027.5 85 697.5 86 503.5 87 388.5 88 322.5 89 271.0 90 223.5 91 167.0 92 122.5 93 106.5 94 90.0 95 70.0 96 49.0 97 27.5 98 17.0 99 9.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15821590019258222 2 0.1322330560046234 3 0.13126984759307894 4 0.14043221896462232 5 0.14642551574756565 6 0.09844941282934147 7 0.0865579509584221 8 0.09575005098464277 9 0.1074571951965629 10-11 0.08030898774525397 12-13 0.09231044563847934 14-15 0.12607327875548716 16-17 0.1257492364195046 18-19 0.12525574075186147 20-21 0.1496956677620685 22-23 0.1274407968706429 24-25 0.1140331736111813 26-27 0.11733900001129689 28-29 0.10559023568282855 30-31 0.0851636770540568 32-33 0.1043297407245111 34-35 0.1219528872172136 36-37 0.11956567624662655 38-39 0.11311455818165278 40-41 0.15712485856592537 42-43 0.12068644652796069 44-45 0.11892651017106463 46-47 0.1535603928701173 48-49 0.16067743279986252 50-51 0.1310974213959506 52-53 0.0978043010228441 54-55 0.09575599671557823 56-57 0.1014103868352004 58-59 0.10188901817550489 60-61 0.0970491931940407 62-63 0.08761926393040166 64-65 0.08772034135630447 66-67 0.10624426608572912 68-69 0.10245088974890583 70-71 0.09901723013367787 72-73 0.11142597059598223 74-75 0.15045969418727506 76-77 0.16593048608134117 78-79 0.1541282101744537 80-81 0.1276994361663354 82-83 0.1063810178972447 84-85 0.11125949012978936 86-87 0.11112868404920925 88-89 0.10730557905770867 90-91 0.11570986973498093 92-93 0.10226062635897111 94-95 0.10351517558635312 96-97 0.09776565377176359 98-99 0.09743566570484559 100-101 0.09378498691047334 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.681879E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.755187286225464 #Duplication Level Percentage of deduplicated Percentage of total 1 78.51668876626272 46.1326275355604 2 12.577889258090288 14.780324780489964 3 3.776729213939166 6.657072968830642 4 1.8765549184734227 4.41029342751174 5 0.900122321241927 2.644342778254071 6 0.6065370228381961 2.138231782373269 7 0.3096038401280648 1.2733582127881335 8 0.25255332074591175 1.1871054128147371 9 0.1917996697062743 1.0142302963525496 >10 0.8783077819981657 9.144597477202497 >50 0.06640477780816628 2.6616019718356205 >100 0.04154698704049302 4.7831834199730165 >500 0.003580449312100132 1.4258709750047718 >1k 0.001661023416183579 1.6437616903689847 >5k 2.0648998821370566E-5 0.10339727063952667 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.06411876240799724 3.567438561275811E-5 0.0 2 0.06420794837202914 3.567438561275811E-5 0.0 3 0.06447550626412482 3.567438561275811E-5 0.0 4 0.06573005549150682 3.567438561275811E-5 1.189146187091937E-5 5 0.06596193899798974 3.567438561275811E-5 1.189146187091937E-5 6 0.06655651209153572 4.756584748367748E-5 3.567438561275811E-5 7 0.06668137244118037 4.756584748367748E-5 3.567438561275811E-5 8 0.06715703091601714 4.756584748367748E-5 4.16201165482178E-5 9 0.06761485219804754 4.756584748367748E-5 4.756584748367748E-5 10-11 0.0686969752283012 6.837590575778638E-5 5.351157841913717E-5 12-13 0.06983558270244174 8.918596403189528E-5 5.9457309354596854E-5 14-15 0.07117634502838789 9.513169496735497E-5 6.540304029005654E-5 16-17 0.07241008419749578 1.1296888777373402E-4 6.837590575778638E-5 18-19 0.07369436207955507 1.1594175324146387E-4 8.621309856416544E-5 20-21 0.0749726942306789 1.248603496446534E-4 9.513169496735497E-5 22-23 0.0761083288393517 1.4864327338649213E-4 1.248603496446534E-4 24-25 0.07706856438542845 1.605347352574115E-4 1.3675181151557276E-4 26-27 0.07803474566244065 1.664804661928712E-4 1.51616138854222E-4 28-29 0.07896227968837236 1.6945333166060102E-4 1.664804661928712E-4 30-31 0.0797441433063853 1.7539906259606073E-4 1.7539906259606073E-4 32-33 0.08058843709922058 1.813447935315204E-4 2.0512771727335913E-4 34-35 0.0814267851611204 2.021548518056293E-4 2.1999204461200836E-4 36-37 0.08219973018273015 2.4674783382157696E-4 2.2296491007973822E-4 38-39 0.08311239988132321 2.6161216116022616E-4 2.2891064101519787E-4 40-41 0.08401317811804535 2.675578920956858E-4 2.497206992893068E-4 42-43 0.08481287892886469 2.9134081583752456E-4 2.556664302247665E-4 44-45 0.08567500991450633 3.0323227770844393E-4 2.705307575634157E-4 46-47 0.08666497411526036 3.0620514317617376E-4 2.7647648849887536E-4 48-49 0.0876430468541435 3.2404233598255284E-4 2.794493539666052E-4 50-51 0.08874300707720353 3.299880669180125E-4 2.794493539666052E-4 52-53 0.08989945174415044 3.418795287889319E-4 2.794493539666052E-4 54-55 0.09100833056361368 3.507981251921214E-4 2.8836795036979473E-4 56-57 0.09225396119459248 3.5377099065985124E-4 2.9728654677298427E-4 58-59 0.09366309942629641 3.745810489339602E-4 3.03232277708444E-4 60-61 0.09537249707024109 4.043097036112586E-4 3.151237395793633E-4 62-63 0.09708189471418574 4.310654928208272E-4 3.21069470514823E-4 64-65 0.09901723013367787 4.489026856272062E-4 3.2701520145028267E-4 66-67 0.10091094543662177 4.637670129658554E-4 3.2701520145028267E-4 68-69 0.10323572623238651 4.816042057722345E-4 3.359337978534722E-4 70-71 0.10560510001016721 5.024142640463435E-4 3.478252597243916E-4 72-73 0.10808149694478617 5.08359994981803E-4 3.626895870630408E-4 74-75 0.11089977340819406 5.232243223204523E-4 3.716081834662303E-4 76-77 0.11409560378600364 5.29170053255912E-4 3.9241824174033924E-4 78-79 0.1176184493652635 5.351157841913716E-4 3.9836397267579895E-4 80-81 0.12157533330281192 5.470072460622911E-4 4.16201165482178E-4 82-83 0.12676595640946822 5.559258424654806E-4 4.2809262735309735E-4 84-85 0.13327058605286113 5.707901698041298E-4 4.5782128203039575E-4 86-87 0.14077707135887896 5.826816316750491E-4 4.6376701296585546E-4 88-89 0.14938946261889233 6.06464555416888E-4 4.697127439013151E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 3190 0.0 26.209488 1 CGCGTGT 2495 0.0 19.802086 3 TAAGTCG 4175 0.0 19.22641 9 GCGTATT 4320 0.0 19.022285 2 ACATGCG 4175 0.0 18.092094 4 CATGCGT 4480 0.0 16.754189 5 GCCGTAT 4515 0.0 15.415322 48-49 CCGTATC 5350 0.0 13.986194 48-49 ATGCGTG 6010 0.0 13.589002 6 CGTATTT 6795 0.0 13.423321 3 GTATTTA 6825 0.0 13.294711 4 TCCGTAC 4065 0.0 13.205723 5 TTAAGTC 6200 0.0 13.174878 8 GTCCGTA 4135 0.0 13.097172 4 GAGTGTA 5010 0.0 13.081815 28-29 CGCGCGT 5300 0.0 12.817304 1 TCGTGTA 5685 0.0 12.660071 14-15 AGAGCGT 9980 0.0 12.611982 9 TGCGTGC 6830 0.0 12.583209 7 CGCCGTA 6120 0.0 12.423597 46-47 >>END_MODULE