##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l07n02 k12-2.3420000000cb33.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 22782943 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.118915804687745 33.0 33.0 33.0 33.0 33.0 2 31.618273372320687 33.0 33.0 33.0 27.0 33.0 3 31.690435427942738 33.0 33.0 33.0 27.0 33.0 4 35.5803708063528 37.0 37.0 37.0 33.0 37.0 5 35.74356175143834 37.0 37.0 37.0 33.0 37.0 6 35.35282149457162 37.0 37.0 37.0 33.0 37.0 7 35.68154092296153 37.0 37.0 37.0 33.0 37.0 8 35.44979943987043 37.0 37.0 37.0 33.0 37.0 9 35.2503243764425 37.0 37.0 37.0 33.0 37.0 10-11 35.50831762604155 37.0 37.0 37.0 33.0 37.0 12-13 35.78292246967391 37.0 37.0 37.0 33.0 37.0 14-15 37.77902431217951 40.0 37.0 40.0 33.0 40.0 16-17 37.84708926761569 40.0 37.0 40.0 33.0 40.0 18-19 37.90897828695792 40.0 37.0 40.0 33.0 40.0 20-21 37.71819768850758 40.0 37.0 40.0 33.0 40.0 22-23 37.635115467742686 40.0 37.0 40.0 33.0 40.0 24-25 37.7582048333264 40.0 37.0 40.0 33.0 40.0 26-27 37.777917387582455 40.0 37.0 40.0 33.0 40.0 28-29 37.61308446849909 40.0 37.0 40.0 33.0 40.0 30-31 37.591594466088075 40.0 37.0 40.0 33.0 40.0 32-33 37.611515751059905 40.0 37.0 40.0 33.0 40.0 34-35 37.389445516323335 40.0 37.0 40.0 33.0 40.0 36-37 37.424381893945835 40.0 37.0 40.0 33.0 40.0 38-39 37.37628692219438 40.0 37.0 40.0 33.0 40.0 40-41 37.28485948896066 40.0 37.0 40.0 33.0 40.0 42-43 37.206976245342844 40.0 37.0 40.0 33.0 40.0 44-45 37.06582685564372 40.0 37.0 40.0 33.0 40.0 46-47 36.92428728369289 40.0 37.0 40.0 33.0 40.0 48-49 36.777904044266805 40.0 37.0 40.0 33.0 40.0 50-51 35.34160099509532 37.0 35.0 38.5 30.0 38.5 52-53 35.6672067124954 37.0 35.0 38.5 30.0 40.0 54-55 36.339013730579055 37.0 37.0 40.0 33.0 40.0 56-57 36.2676829986363 37.0 37.0 40.0 33.0 40.0 58-59 36.13616873377596 37.0 37.0 40.0 33.0 40.0 60-61 35.96270969031525 37.0 37.0 40.0 33.0 40.0 62-63 35.7815049179555 37.0 37.0 40.0 33.0 40.0 64-65 35.62149968948261 37.0 37.0 40.0 27.0 40.0 66-67 35.42584632283898 37.0 33.0 37.0 27.0 40.0 68-69 35.243783606007355 37.0 33.0 37.0 27.0 40.0 70-71 35.03607505843297 37.0 33.0 37.0 27.0 40.0 72-73 34.82532868997653 37.0 33.0 37.0 27.0 40.0 74-75 34.61661671628639 37.0 33.0 37.0 27.0 37.0 76-77 34.42964352761625 37.0 33.0 37.0 27.0 37.0 78-79 34.280200060194154 37.0 33.0 37.0 27.0 37.0 80-81 34.15462695052172 37.0 33.0 37.0 27.0 37.0 82-83 34.0563716241576 37.0 33.0 37.0 27.0 37.0 84-85 33.95321491169951 37.0 33.0 37.0 27.0 37.0 86-87 33.85262863098942 37.0 33.0 37.0 27.0 37.0 88-89 33.76364030318646 37.0 33.0 37.0 27.0 37.0 90-91 33.66900867460363 37.0 33.0 37.0 27.0 37.0 92-93 33.58492548570217 37.0 33.0 37.0 27.0 37.0 94-95 33.50610908783821 37.0 33.0 37.0 27.0 37.0 96-97 33.42354988993301 37.0 33.0 37.0 27.0 37.0 98-99 33.3595795767035 37.0 33.0 37.0 27.0 37.0 100-101 31.562043586730653 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 2306 1 0.46408990255442717 2306 2 0.832235645075631 2306 3 0.7558662212959035 2306 4 0.7740939936640956 2306 5 0.6524265917650567 2306 6 0.9545843893460173 2306 7 0.7167586101101548 2306 8 0.9074442854608122 2306 9 1.0216579007837439 2306 10-11 0.8216010884381078 2306 12-13 0.6157410959237382 2306 14-15 0.9896859548615211 2306 16-17 0.950753654394056 2306 18-19 0.886853814807381 2306 20-21 0.9446913903039658 2306 22-23 1.045850452616719 2306 24-25 0.9824669137408861 2306 26-27 0.8922742346213823 2306 28-29 1.0138318688248802 2306 30-31 0.9435624301611156 2306 32-33 0.9203644015910513 2306 34-35 1.053096418978086 2306 36-37 0.937085050037048 2306 38-39 0.9082544295525636 2306 40-41 0.853514896050946 2306 42-43 0.8591765885586398 2306 44-45 0.8601661508035505 2306 46-47 0.8493348933225349 2306 48-49 0.8995261892940292 2306 50-51 0.8666244642281669 2306 52-53 0.8432767268952972 2306 54-55 0.9161338817353055 2306 56-57 0.8886624465937558 2306 58-59 0.8945382449998434 2306 60-61 0.8827422072994509 2306 62-63 0.9042960340876292 2306 64-65 0.8944685194537882 2306 66-67 0.8817179594280731 2306 68-69 0.8667773008055875 2306 70-71 0.8320223662457025 2306 72-73 0.8476472281271228 2306 74-75 0.7912015514253383 2306 76-77 0.8328487767844805 2306 78-79 0.7928345948432636 2306 80-81 0.7880577250924148 2306 82-83 0.7931124407067855 2306 84-85 0.865248384926069 2306 86-87 0.8269038167612948 2306 88-89 0.8379708481016124 2306 90-91 0.87018790276732 2306 92-93 0.8030427795767707 2306 94-95 0.8456823617482812 2306 96-97 0.8721521790397091 2306 98-99 0.765529055566752 2306 100-101 0.6845948893656519 2307 1 0.2998496295949309 2307 2 0.7023782994648151 2307 3 0.648202544761773 2307 4 0.6931466434251732 2307 5 0.5404094604454315 2307 6 0.8145283213719026 2307 7 0.6087160918033092 2307 8 0.7861214613033027 2307 9 0.8945922071417556 2307 10-11 0.7329244232555467 2307 12-13 0.47099972784331356 2307 14-15 0.8198867990124583 2307 16-17 0.7811157766824266 2307 18-19 0.6651055584289622 2307 20-21 0.7969591547185431 2307 22-23 0.91366810461939 2307 24-25 0.7482385038833286 2307 26-27 0.6162700466549254 2307 28-29 0.8234471607925116 2307 30-31 0.7768327480159982 2307 32-33 0.7516562305953727 2307 34-35 0.7975949135767095 2307 36-37 0.7221298499441744 2307 38-39 0.6831175702773393 2307 40-41 0.6403399842817734 2307 42-43 0.6908536021935205 2307 44-45 0.6641322240525511 2307 46-47 0.6080647137826247 2307 48-49 0.679996882034807 2307 50-51 0.5639680305077945 2307 52-53 0.6835938685706751 2307 54-55 0.7167451401129483 2307 56-57 0.7127404618367805 2307 58-59 0.6503663743164267 2307 60-61 0.7069610028138982 2307 62-63 0.7332180748449773 2307 64-65 0.6708219485561244 2307 66-67 0.6308382324170694 2307 68-69 0.6112914653233119 2307 70-71 0.6706393787225622 2307 72-73 0.6797758942965757 2307 74-75 0.589440657002477 2307 76-77 0.6538840982228962 2307 78-79 0.6922760994629655 2307 80-81 0.6295170639732106 2307 82-83 0.6453614407543924 2307 84-85 0.6595120506569998 2307 86-87 0.6547006056466458 2307 88-89 0.593594403621239 2307 90-91 0.6174802143078395 2307 92-93 0.6077279565134006 2307 94-95 0.5847604252897938 2307 96-97 0.6232868691677993 2307 98-99 0.6369604144422496 2307 100-101 0.48014758841550886 2201 1 0.30302133173489665 2201 2 0.7260250575401805 2201 3 0.6992638463416796 2201 4 0.6928186183893885 2201 5 0.5655699007322212 2201 6 0.8510829564001767 2201 7 0.6416077829255258 2201 8 0.8164364585634729 2201 9 0.9694091291415745 2201 10-11 0.7768334705432309 2201 12-13 0.5501960399095864 2201 14-15 0.8385062039665527 2201 16-17 0.798148702313199 2201 18-19 0.7948817781803115 2201 20-21 0.8919700102209376 2201 22-23 0.9846189858562298 2201 24-25 0.8465227460757134 2201 26-27 0.7753787871148461 2201 28-29 0.8962744375872802 2201 30-31 0.8792764340120556 2201 32-33 0.8330433999635645 2201 34-35 0.9499580629031215 2201 36-37 0.8562870775143452 2201 38-39 0.8370052496563645 2201 40-41 0.7954762561158901 2201 42-43 0.7926520178984262 2201 44-45 0.7859345405825167 2201 46-47 0.794889220796847 2201 48-49 0.8223001629703077 2201 50-51 0.4846856669290531 2201 52-53 0.6370249082840047 2201 54-55 0.8220750289844574 2201 56-57 0.8028166176260072 2201 58-59 0.8470280967339292 2201 60-61 0.8782818383661422 2201 62-63 0.8194726841696607 2201 64-65 0.8165493463765685 2201 66-67 0.8352343469367511 2201 68-69 0.8143684729298428 2201 70-71 0.8457058834408713 2201 72-73 0.862761478405659 2201 74-75 0.8744824239309636 2201 76-77 0.8098250194975876 2201 78-79 0.8517895013966097 2201 80-81 0.8557323772374872 2201 82-83 0.8478966653578794 2201 84-85 0.8238069125857379 2201 86-87 0.7856963436841156 2201 88-89 0.8022135848124279 2201 90-91 0.8331024308946198 2201 92-93 0.7940733610123871 2201 94-95 0.7997395844333397 2201 96-97 0.8131152160071693 2201 98-99 0.7617205285524662 2201 100-101 0.6672988587233597 1101 1 0.3514104811471199 1101 2 0.7773720846965695 1101 3 0.7110992265486544 1101 4 0.7499257425526835 1101 5 0.5889634086545215 1101 6 0.8863801985115529 1101 7 0.6664928754969708 1101 8 0.8903078941022144 1101 9 1.0236291650526823 1101 10-11 0.8179637733269729 1101 12-13 0.5997599743886113 1101 14-15 0.9784970921106932 1101 16-17 0.8749951149806492 1101 18-19 0.8558039307476832 1101 20-21 0.9487236497075173 1101 22-23 1.0632317947100987 1101 24-25 0.9718260487229244 1101 26-27 0.8872751886406576 1101 28-29 0.972732072462847 1101 30-31 0.9418666858050102 1101 32-33 0.900477899048802 1101 34-35 1.030940566606695 1101 36-37 0.9553171522211343 1101 38-39 0.9030803041145177 1101 40-41 0.8618598962087702 1101 42-43 0.9043476677133597 1101 44-45 0.9117162376219881 1101 46-47 0.8898559231301277 1101 48-49 0.8882694161208775 1101 50-51 0.7829024631837243 1101 52-53 0.8587646182511435 1101 54-55 0.9749493578508535 1101 56-57 1.0175627119828476 1101 58-59 1.0150300670795716 1101 60-61 1.0483819913960204 1101 62-63 0.9959129557835311 1101 64-65 1.013066977769391 1101 66-67 0.9339621098967612 1101 68-69 0.9127228406476675 1101 70-71 0.9518527140084245 1101 72-73 0.9471720947431166 1101 74-75 0.9610801172483079 1101 76-77 0.990779512296811 1101 78-79 1.0136140640922875 1101 80-81 0.9578198335151882 1101 82-83 0.9345871185714927 1101 84-85 0.9613052795963313 1101 86-87 0.9290000207102125 1101 88-89 0.9559729692566563 1101 90-91 0.9451388960163527 1101 92-93 0.9923629710270703 1101 94-95 0.9773623033514909 1101 96-97 0.9769428549078825 1101 98-99 0.9845887797509718 1101 100-101 0.960126338034673 2304 1 0.3213612037369984 2304 2 0.6664348735521095 2304 3 0.5968868283521687 2304 4 0.5749720570274945 2304 5 0.43346207154269933 2304 6 0.7688682036406007 2304 7 0.5760298200798459 2304 8 0.748309832508987 2304 9 0.9075658456162756 2304 10-11 0.6650882097036614 2304 12-13 0.4514896501187238 2304 14-15 0.7532082784839034 2304 16-17 0.6572589807221689 2304 18-19 0.6172867814052267 2304 20-21 0.7421943127944743 2304 22-23 0.8281054027861003 2304 24-25 0.6746069540574311 2304 26-27 0.6705520036681136 2304 28-29 0.7345768636470922 2304 30-31 0.7170071956490887 2304 32-33 0.6789501034076082 2304 34-35 0.7277571539798302 2304 36-37 0.7033215310896992 2304 38-39 0.6793994665464282 2304 40-41 0.6806265757003942 2304 42-43 0.6277935423345369 2304 44-45 0.6807998893855824 2304 46-47 0.6552560333750392 2304 48-49 0.648579613377656 2304 50-51 0.605993045803757 2304 52-53 0.5948303052082338 2304 54-55 0.654981999559034 2304 56-57 0.675966101099398 2304 58-59 0.7128201210805116 2304 60-61 0.6945236364439253 2304 62-63 0.6265674585844252 2304 64-65 0.6589952294188635 2304 66-67 0.6779411593592855 2304 68-69 0.718413237854655 2304 70-71 0.7212082684474126 2304 72-73 0.7526139104106591 2304 74-75 0.725419200259239 2304 76-77 0.7527311941060333 2304 78-79 0.7414428234218633 2304 80-81 0.7087093396746127 2304 82-83 0.7202430960839123 2304 84-85 0.7406606812718692 2304 86-87 0.734230132010083 2304 88-89 0.7306555609645216 2304 90-91 0.7422774895987132 2304 92-93 0.7170506679868325 2304 94-95 0.7162906753850606 2304 96-97 0.7066619743458347 2304 98-99 0.629799305925836 2304 100-101 0.6529650202323474 2202 1 0.1892355874886391 2202 2 0.5800231403546157 2202 3 0.5633821664177887 2202 4 0.5401034350392422 2202 5 0.39156649042082137 2202 6 0.6267160135747787 2202 7 0.49594379584515025 2202 8 0.6455222009699071 2202 9 0.8163064813019005 2202 10-11 0.6397545657099215 2202 12-13 0.4423365630180669 2202 14-15 0.7308803794885179 2202 16-17 0.6563963816366538 2202 18-19 0.5588733773320982 2202 20-21 0.7080888270441008 2202 22-23 0.8097203703385034 2202 24-25 0.6343188859291402 2202 26-27 0.5911227613384753 2202 28-29 0.6828904090638446 2202 30-31 0.6776683507205874 2202 32-33 0.5928472248498977 2202 34-35 0.7256998589667347 2202 36-37 0.5962916713176654 2202 38-39 0.6057608904084972 2202 40-41 0.5919049664876752 2202 42-43 0.6206335734415589 2202 44-45 0.6076840592581831 2202 46-47 0.607507020794074 2202 48-49 0.5870073244727578 2202 50-51 0.4305880521752812 2202 52-53 0.49994357718549054 2202 54-55 0.6373335153683044 2202 56-57 0.654023342425873 2202 58-59 0.675099317870469 2202 60-61 0.6893430341022011 2202 62-63 0.6833054079541867 2202 64-65 0.734980058786121 2202 66-67 0.7221256313097015 2202 68-69 0.7028476081141122 2202 70-71 0.7036991459372146 2202 72-73 0.6914307892876934 2202 74-75 0.7037833282620554 2202 76-77 0.7422479516014064 2202 78-79 0.6768400586977066 2202 80-81 0.71362081277816 2202 82-83 0.6842210861302433 2202 84-85 0.7284604400424257 2202 86-87 0.7336843316811681 2202 88-89 0.7541510042547657 2202 90-91 0.7404761454541458 2202 92-93 0.712512787571427 2202 94-95 0.7220638515124307 2202 96-97 0.675072413032872 2202 98-99 0.6989377229550158 2202 100-101 0.61643892017619 1102 1 0.2119344883484473 1102 2 0.5433915017180162 1102 3 0.49281800791731456 1102 4 0.5039539832362365 1102 5 0.3122361166823566 1102 6 0.5895275875456747 1102 7 0.4624851417279956 1102 8 0.64490810949993 1102 9 0.7375845378495214 1102 10-11 0.570067068086523 1102 12-13 0.35994663705781704 1102 14-15 0.6084051513404347 1102 16-17 0.5139495445628555 1102 18-19 0.43108458758757706 1102 20-21 0.5870690470762057 1102 22-23 0.6444029193095915 1102 24-25 0.5122906708351849 1102 26-27 0.38237971997182996 1102 28-29 0.5193133429882266 1102 30-31 0.5169069460475342 1102 32-33 0.4797220890929097 1102 34-35 0.6167574111690541 1102 36-37 0.4805960925581445 1102 38-39 0.48493247647326854 1102 40-41 0.5111133553684155 1102 42-43 0.4690046801472434 1102 44-45 0.4519360549013669 1102 46-47 0.33931687596505355 1102 48-49 0.4873229297850301 1102 50-51 0.3211978824901607 1102 52-53 0.40034685735699327 1102 54-55 0.4802290387221859 1102 56-57 0.5147850002875529 1102 58-59 0.530177016718504 1102 60-61 0.5418055504743577 1102 62-63 0.560389304442559 1102 64-65 0.49011197176729837 1102 66-67 0.5568205078169015 1102 68-69 0.5349099677331068 1102 70-71 0.5455492918582578 1102 72-73 0.6082220660083166 1102 74-75 0.5767685939926253 1102 76-77 0.5700625883960626 1102 78-79 0.5881630585023387 1102 80-81 0.585922123098527 1102 82-83 0.5763697390709766 1102 84-85 0.6068691626124973 1102 86-87 0.5843040663178627 1102 88-89 0.5746022377831181 1102 90-91 0.586033423292939 1102 92-93 0.5436621248231717 1102 94-95 0.5702501423743485 1102 96-97 0.4465860212956798 1102 98-99 0.4682024140638461 1102 100-101 0.546814831131325 2305 1 0.2590818679500657 2305 2 0.4639529054942564 2305 3 0.5006230039001309 2305 4 0.43205505002735123 2305 5 0.2582401230041995 2305 6 0.6048148564423954 2305 7 0.3508410739535677 2305 8 0.6080727762524702 2305 9 0.7448045192655002 2305 10-11 0.5078720636843528 2305 12-13 0.3154034680365001 2305 14-15 0.49164889862348105 2305 16-17 0.48044361999741625 2305 18-19 0.44792692032270054 2305 20-21 0.554375756458974 2305 22-23 0.5599331926776827 2305 24-25 0.45699388727057766 2305 26-27 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-1.3031354384192397 1213 70-71 -1.2217457795756133 1213 72-73 -1.1749879801937837 1213 74-75 -1.3877191930561352 1213 76-77 -1.351979534669887 1213 78-79 -1.2887871572160634 1213 80-81 -1.113493645893925 1213 82-83 -1.0290806452943784 1213 84-85 -1.1174052119684035 1213 86-87 -1.1056879353731404 1213 88-89 -1.2147653617594756 1213 90-91 -1.227348323175491 1213 92-93 -1.2984905876687591 1213 94-95 -1.1012535780964967 1213 96-97 -1.265252534440954 1213 98-99 -1.174310264724575 1213 100-101 -1.0149576013977288 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 18325.0 3 1488.0 4 892.0 5 697.0 6 693.0 7 1426.0 8 2969.0 9 8222.0 10 26264.0 11 35004.0 12 29345.0 13 30519.0 14 35066.0 15 40683.0 16 48385.0 17 57800.0 18 65777.0 19 74353.0 20 82972.0 21 92750.0 22 102453.0 23 114417.0 24 128409.0 25 145422.0 26 168889.0 27 202139.0 28 234409.0 29 274643.0 30 336658.0 31 422371.0 32 535426.0 33 724851.0 34 1010910.0 35 1508807.0 36 3033672.0 37 7259996.0 38 5921095.0 39 4746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.445402494527197 12.142291648432044 10.336887031471665 56.07541882556909 2 22.00430151419681 19.15315625812654 39.66132860331143 19.18121362436522 3 24.31813990707833 23.78792928896059 23.695795179246637 28.198135624714443 4 28.038194459705906 28.14562000375183 17.15302573378226 26.66315980276 5 28.950032127108184 29.554983508318895 21.105387024621873 20.389597339951045 6 20.896399054660026 32.371580716976545 22.726207104530786 24.00581312383265 7 19.54104995621529 14.378513221119949 41.151596312261645 24.92884051040312 8 21.108555637565022 19.70080750035147 27.415625439336427 31.775011422747085 9 22.27445805959499 19.25166176310341 30.465363970937158 28.008516206364437 10-11 25.6151225046248 27.74983666291625 20.816878917204555 25.818161915254397 12-13 23.541174974829097 22.008930607211173 27.34470723949335 27.105187178466377 14-15 24.39646308962249 23.505803862174783 25.79799657816026 26.29973647004247 16-17 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24.21025383193407 24.39056540163999 25.893226290822156 42-43 25.17797333872618 24.104178852971888 24.657729889031398 26.06011791927053 44-45 25.45040594744342 24.301299429510443 24.645214689142023 25.60307993390411 46-47 25.5424234961957 24.18397890907179 24.344748266914852 25.92884932781766 48-49 25.251794417972008 24.076416026250573 24.380817543290455 26.290972012486957 50-51 25.335424748657363 24.326364685965444 24.632691356492444 25.70551920888475 52-53 25.509299123584313 24.241942362431978 24.44948825483121 25.7992702591525 54-55 25.066004716445846 24.131436363237764 24.57459441285205 26.227964507464335 56-57 25.06230844025113 24.41126643891543 24.990062217602492 25.53636290323094 58-59 25.345301435431928 24.39964503861946 24.403030330656918 25.852023195291697 60-61 25.02372287021764 24.617407552968228 24.422150378426927 25.93671919838721 62-63 25.18031703184016 24.701678360963363 24.588701535500796 25.529303071695686 64-65 25.33553458854258 24.64017859213853 24.285171935832363 25.739114883486526 66-67 25.129126163233924 24.543478144815378 24.387892940243354 25.939502751707344 68-69 25.243262259532116 24.70664328529995 24.518649030114407 25.53144542505353 70-71 25.509596049962663 24.550987284166997 24.21598364468556 25.723433021184782 72-73 25.220667762435845 24.51021642213839 24.41379597017568 25.855319845250087 74-75 25.316814979313595 24.7082539900234 24.517047602469358 25.457883428193647 76-77 25.504463854413288 24.58004311353447 24.213864937230603 25.701628094821636 78-79 25.357272339734816 24.501159032081553 24.350015050437925 25.791553577745706 80-81 25.441577641682233 24.756342371617983 24.358173137509095 25.44390684919069 82-83 25.627550056460436 24.606202739100063 24.089405210177816 25.676841994261686 84-85 25.30193081358427 24.56459943310425 24.37539910148682 25.75807065182466 86-87 25.47519771636533 24.80789262515958 24.380783269047082 25.336126389428017 88-89 25.564358362422052 24.5672765750271 24.1158237179102 25.752541344640644 90-91 25.48698918605467 24.431188918792444 24.210694615279778 25.871127279873107 92-93 25.507947261502245 24.709384380315665 24.364840887263092 25.417827470918997 94-95 25.737458290854466 24.56640030502842 24.10250877570725 25.593632628409864 96-97 25.44447897697936 24.597794820857864 24.24411751887339 25.71360868328938 98-99 25.611038737901236 24.738428931102963 24.30285333701737 25.347678993978427 100-101 25.97107586108033 24.566591851383578 23.88911144489753 25.573220842638566 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 16266.0 1 8631.0 2 820.5 3 562.0 4 465.0 5 443.0 6 432.0 7 417.5 8 397.5 9 387.5 10 404.0 11 440.5 12 476.5 13 523.0 14 594.5 15 698.0 16 806.5 17 949.5 18 1190.0 19 1543.5 20 2073.0 21 2796.0 22 3802.0 23 5417.0 24 8020.5 25 11837.0 26 17073.5 27 23904.5 28 32882.5 29 44676.5 30 59632.0 31 78715.0 32 101761.0 33 130226.5 34 167806.0 35 216306.5 36 274863.0 37 345950.0 38 430901.5 39 522486.0 40 615268.0 41 703416.0 42 782011.5 43 838677.0 44 870300.0 45 879602.0 46 869038.0 47 842898.0 48 805096.5 49 767347.5 50 731749.5 51 699213.5 52 678486.0 53 671540.5 54 671830.0 55 673457.0 56 671328.5 57 666917.5 58 649392.0 59 625998.0 60 603974.0 61 577148.0 62 552011.5 63 512223.5 64 456382.0 65 411265.5 66 383302.5 67 357598.0 68 324970.5 69 285671.5 70 244475.0 71 202447.0 72 165168.0 73 134927.0 74 103631.5 75 75834.5 76 54897.5 77 38359.5 78 26122.0 79 17609.5 80 11464.0 81 7033.0 82 4315.5 83 2594.0 84 1602.0 85 1120.0 86 829.0 87 669.0 88 531.0 89 434.5 90 351.5 91 264.0 92 199.0 93 152.5 94 117.0 95 87.0 96 62.0 97 42.0 98 24.5 99 10.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15566908980986346 2 0.12974618775107324 3 0.12796854207992356 4 0.13769511691268332 5 0.14346258953463562 6 0.09669953526197209 7 0.08466421568100312 8 0.09367973224530299 9 0.10526734847205647 10-11 0.07858730103481362 12-13 0.09019466888013547 14-15 0.12388434628485002 16-17 0.12318645576210238 18-19 0.12279142339073577 20-21 0.14638363445846306 22-23 0.12468538414901006 24-25 0.11186658369816403 26-27 0.11456158232059836 28-29 0.10350067592233365 30-31 0.0829172947498486 32-33 0.1022694916982411 34-35 0.11970797626979096 36-37 0.11710295724305679 38-39 0.1108504726540377 40-41 0.15404506783868965 42-43 0.11792155210149981 44-45 0.11680009909167573 46-47 0.15013424736216036 48-49 0.1580700087780582 50-51 0.1285720637584003 52-53 0.09529936496790603 54-55 0.09354366553961005 56-57 0.09912459509730592 58-59 0.0997259221514973 60-61 0.09515671438935698 62-63 0.08526115348662375 64-65 0.08588442678366881 66-67 0.1040668889879591 68-69 0.10068497296420396 70-71 0.09720210422332182 72-73 0.10887311617291938 74-75 0.14793084457964892 76-77 0.16321640272725083 78-79 0.15114596915771591 80-81 0.12475341750185653 82-83 0.10377500395800489 84-85 0.10881166669292901 86-87 0.10901576675146842 88-89 0.10524320760491741 90-91 0.11364861861788444 92-93 0.09955474145723843 94-95 0.10137627961409551 96-97 0.0956812295935604 98-99 0.09516549288649846 100-101 0.09165848327847724 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.2782943E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.28023868539849 #Duplication Level Percentage of deduplicated Percentage of total 1 74.90842718056638 39.162304525478014 2 13.072710601582624 13.668888610317575 3 4.536476811424355 7.115042714761222 4 2.363662192231588 4.942912943260787 5 1.3067785960447664 3.4159348455095153 6 0.8594582744007487 2.695961023548711 7 0.5093150608129102 1.863897906175703 8 0.4130428365392614 1.7275182465253291 9 0.30381036689994345 1.4294950646964777 >10 1.5823298058016462 14.489269481396924 >50 0.09581397802642234 3.4948196255395567 >100 0.04535449146359912 4.442845049761199 >500 0.0020193825486867776 0.6992542426344713 >1k 7.546698488553349E-4 0.6254561757473125 >5k 2.8729902141433218E-5 0.09159674673395216 >10k+ 1.7021906194529932E-5 0.1348027979132849 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.09549249190501859 2.6335491424439768E-5 4.389248570739961E-6 2 0.09591824901638037 2.6335491424439768E-5 2.1946242853699806E-5 3 0.09759933121897377 3.511398856591969E-5 2.1946242853699806E-5 4 0.1035116490437605 4.389248570739961E-5 2.1946242853699806E-5 5 0.10460457193787476 4.389248570739961E-5 2.1946242853699806E-5 6 0.10760242871169015 6.583872856109942E-5 2.6335491424439768E-5 7 0.1082344805058767 7.022797713183938E-5 2.6335491424439768E-5 8 0.10980144224563086 7.461722570257934E-5 3.072473999517973E-5 9 0.11199606653100085 7.461722570257934E-5 3.511398856591969E-5 10-11 0.11665744851312668 7.681184998794932E-5 3.511398856591969E-5 12-13 0.12139564234524047 1.0314734141238909E-4 3.950323713665965E-5 14-15 0.12631160074446923 1.1192583855386901E-4 4.169786142202963E-5 16-17 0.1315391957922205 1.1850971140997895E-4 4.389248570739961E-5 18-19 0.13621374552005858 1.250935842660889E-4 4.608710999276959E-5 20-21 0.14177053421061536 1.4265057854904875E-4 4.8281734278139574E-5 22-23 0.14658115064414637 1.6459682140274853E-4 4.8281734278139574E-5 24-25 0.15002012689932115 1.8215381568570838E-4 5.2670982848879535E-5 26-27 0.15371148494731343 1.931269371125583E-4 5.2670982848879535E-5 28-29 0.15673567721255327 2.0190543425403822E-4 5.2670982848879535E-5 30-31 0.15929680375358002 2.0629468282477818E-4 6.364410427572944E-5 32-33 0.16142778393467427 2.26046301393108E-4 7.461722570257934E-5 34-35 0.16358071035862223 2.370194228199579E-4 1.0095271712701911E-4 36-37 0.16558659695545042 2.4140867139069787E-4 1.1850971140997895E-4 38-39 0.16776805349510815 2.4579791996143783E-4 1.2948283283682884E-4 40-41 0.16989244980334628 2.501871685321778E-4 1.4923445140515868E-4 42-43 0.1719685643773063 2.611602899590277E-4 1.6898606997348852E-4 44-45 0.17421585964552516 2.787172842419875E-4 1.8434843997107837E-4 46-47 0.17632269895948033 2.9627427852494737E-4 1.931269371125583E-4 48-49 0.1783636995448744 3.0944202423716723E-4 1.931269371125583E-4 50-51 0.1806022163159518 3.3358289137623706E-4 1.931269371125583E-4 52-53 0.18285829008131216 3.467506370884569E-4 1.9971080996866822E-4 54-55 0.18522848430951172 3.6430763137141676E-4 2.0848930711014815E-4 56-57 0.18754161830629168 3.6650225565678675E-4 2.1068393139551814E-4 58-59 0.18985255767878628 3.6869687994215675E-4 2.1068393139551814E-4 60-61 0.19261778427835244 3.928377470812265E-4 2.2165705282236806E-4 62-63 0.1955893055607434 4.213678627910363E-4 2.26046301393108E-4 64-65 0.19858496771027342 4.52092602786216E-4 2.3482479853458791E-4 66-67 0.2016881664497866 4.630657242130659E-4 2.4579791996143783E-4 68-69 0.20532246426635925 4.6745497278380583E-4 2.633549142443977E-4 70-71 0.20932984821144485 4.7842809421065575E-4 2.7652265995661753E-4 72-73 0.21428750447209563 4.8720659135213567E-4 2.896904056688374E-4 74-75 0.21999352761405758 5.091528342058354E-4 2.940796542395774E-4 76-77 0.2267749166558508 5.223205799180554E-4 2.940796542395774E-4 78-79 0.23495867061599549 5.289044527741653E-4 3.0066352709568736E-4 80-81 0.24556090053861784 5.420721984863852E-4 3.0944202423716723E-4 82-83 0.25971622717925424 5.508506956278651E-4 3.445560128030869E-4 84-85 0.27820374215921095 5.57434568483975E-4 3.467506370884569E-4 86-87 0.3020527242683265 5.662130656254549E-4 3.533345099445669E-4 88-89 0.3312412272637473 5.881593084791548E-4 3.6430763137141676E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTAAAAA 21505 0.0 16.274792 56-57 AGAGCGT 15600 0.0 13.944585 9 TAAAAAA 24925 0.0 13.831105 58-59 CGAATTA 7275 0.0 13.810377 14-15 CCGTATC 11125 0.0 13.431034 48-49 TTTAAAA 13390 0.0 13.069058 56-57 GCCGTAT 9720 0.0 12.366393 48-49 GAGTGTA 9635 0.0 12.0273075 28-29 CTCGGAT 12100 0.0 11.857189 1 TCGTGTA 11720 0.0 11.673284 14-15 CGCCGTA 12410 0.0 11.659521 46-47 CATTAAA 15405 0.0 11.575187 54-55 GTAGGGA 12290 0.0 11.1707735 18-19 CTCGTTT 9340 0.0 11.139287 1 AGTGTAG 11445 0.0 10.975902 28-29 AAGAGCG 20365 0.0 10.960526 8 ATCGGAA 21900 0.0 10.955285 3 CTCGGTT 10325 0.0 10.95083 1 CTCGATT 12360 0.0 10.723731 1 CTCGTAT 5850 0.0 10.719592 1 >>END_MODULE