FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n02 k12-2.3420000000cb33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n02 k12-2.3420000000cb33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782943
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAAAA215050.016.27479256-57
AGAGCGT156000.013.9445859
TAAAAAA249250.013.83110558-59
CGAATTA72750.013.81037714-15
CCGTATC111250.013.43103448-49
TTTAAAA133900.013.06905856-57
GCCGTAT97200.012.36639348-49
GAGTGTA96350.012.027307528-29
CTCGGAT121000.011.8571891
TCGTGTA117200.011.67328414-15
CGCCGTA124100.011.65952146-47
CATTAAA154050.011.57518754-55
GTAGGGA122900.011.170773518-19
CTCGTTT93400.011.1392871
AGTGTAG114450.010.97590228-29
AAGAGCG203650.010.9605268
ATCGGAA219000.010.9552853
CTCGGTT103250.010.950831
CTCGATT123600.010.7237311
CTCGTAT58500.010.7195921
Produced by FastQC (version 0.11.2)