FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 mx48-3.3410000000cdd4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 mx48-3.3410000000cdd4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14284593
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT739410.5176276285925682TruSeq Adapter, Index 22 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTA330210.2311651441521645TruSeq Adapter, Index 22 (97% over 40bp)
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA171810.12027644049781468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA127900.041.6052936-37
CGTAATC133200.038.87905538-39
CGTACGT138000.038.68198434-35
TCGGAAG200100.038.114053
ATCGGAA199000.038.1100922
ACCGTAC142150.037.93733232-33
GAGCACA203350.037.7138869
TATGCCG141100.037.59134748-49
CGTATGC142550.037.30503546-47
AGAGCAC210050.036.4204948
CACACGT153450.035.6976512-13
CTCGTAT145950.035.67370244-45
CACGTCT166250.032.96319614-15
GATCGGA232700.032.830051
AGTCACC171750.031.53585428-29
TAATCTC168250.030.97675340-41
AGCACAC177950.030.90248710-11
CGGAAGA252200.030.6157844
TCACCGT179350.030.35968430-31
CGTCTGA182050.030.08929816-17
Produced by FastQC (version 0.11.2)