FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 mx24-3.3410000000cc35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 mx24-3.3410000000cc35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19451787
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT202720.10421664600789635TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG108600.013.56141848-49
CACACGT126450.013.36906912-13
CGTATGC110950.013.3579146-47
CGTCTGA134100.012.57081516-17
CTCGTAT121650.012.10613444-45
ATCGGAA196150.011.98366452
CGAATTA69550.011.70931214-15
AGAGCAC210950.011.6826278
CTCGAAT89750.011.5464751
GAGCACA220700.011.5322799
CGGGAAT55500.011.3059841
CGCAAAT44200.011.2926131
CTGGAAT165250.010.9600091
CTCGATT107650.010.9071251
CCGCGTT62800.010.9000951
TTACTCG58600.010.69663
CGCGAAT23600.010.6755741
CCGATTA42750.010.6636173
CTCGGTT81600.010.6607631
CCGGGAT88150.010.5696631
Produced by FastQC (version 0.11.2)