FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 mx0-2.3410000000ca94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 mx0-2.3410000000ca94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23595947
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT17950.030.4572981
TAAGTCG23900.019.2722649
ACATGCG44050.018.2178424
GCGTATT30400.017.8074282
TACCCTA51600.016.6566549
AACCGAT89450.016.5095525
CCGCGTT61300.016.2860891
CTTACGG48500.016.0753361
GTTAGTT54900.015.9332171
AAAGGGC150650.015.7605312
TTACGGT51250.015.1956732
GTCCGTA44550.015.0289134
GCGCTTA81200.015.00848738-39
TGAACCG102500.014.3153453
CTCGGTT94500.014.2368081
CCTAGGC88450.014.1194198
CGCGGTT35250.014.0259481
CGTATGC70350.014.01062346-47
TATGCCG70300.013.98841948-49
CTGGTAT84300.013.9856321
Produced by FastQC (version 0.11.2)