FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 m48-3.3410000000cd1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 m48-3.3410000000cd1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11989871
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC10913349.102132958728246TruSeq Adapter, Index 3 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG2157731.7996273688015492TruSeq Adapter, Index 3 (100% over 49bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT779450.6500903971360492Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAGCAGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATG176200.14695737760648134TruSeq Adapter, Index 3 (97% over 46bp)
CGCGTATTTAAGTCGTCTGCAAAGGATTCGGCCCGTCGTTCGTGCGGAAT163550.13640680537763916No Hit
CTGACATGCGTGCGAGTCGACGGGTGCGGAAACCCGGAAGGCACAAGGAA137530.11470515404210771No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGGC117550.081.880595
GATACGG121900.079.6208954
TGATACG122700.079.0273363
TACGGCG124300.077.4347846
ATGATAC149200.065.055442
TCGGAAG1747000.064.912123
ATCGGAA1758100.064.5949252
GATCGGA1772900.064.083391
CGGAAGA1823900.062.3369524
GAGCACA1909000.059.4269839
AGAGCAC1921950.059.043868
GGAAGAG1960800.058.0718575
GAAGAGC2016550.056.3726736
AAGAGCA2016400.056.3132867
AATGATA182050.053.40141
ACGGCGA183550.052.6975147
GGCGACC211200.046.0009779
TATGCCG1616050.041.55562646-47
ACTTAGG1628400.041.49950432-33
CTCGTAT1606850.041.47467442-43
Produced by FastQC (version 0.11.2)