FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 kx48-3.3410000000cdae.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 kx48-3.3410000000cdae.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15283528
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC660700.4322954752332053TruSeq Adapter, Index 9 (100% over 50bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG257760.1686521593705328TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA182350.035.4434552
AGTCACG130400.034.28610228-29
GAGCACA202100.032.706389
TATGCCG136900.032.5988946-47
CACACGT138700.032.5940612-13
CGTATGC138250.032.52416244-45
GATCGGA201850.032.2182271
AGAGCAC207550.031.7560338
TCGGAAG206200.031.5741063
CTCGTAT142700.030.42950842-43
CACGTCT148950.030.39882314-15
CGGAAGA232300.028.1888544
CGTCTGA165400.027.49032416-17
AGCACAC168250.027.36290610-11
CGCGTAT38100.025.3288441
ACGTCTG133800.024.40018714-15
CCAGTCA188950.023.9106526-27
TCACGAT195500.023.07661430-31
ACGATCA196850.023.0273532-33
CCGTCTT195900.022.96735450-51
Produced by FastQC (version 0.11.2)