FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 kx24-3.3410000000cba0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 kx24-3.3410000000cba0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25266941
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT121450.011.978011
CGGAAAT85400.011.3005341
CGAGAAT100550.011.1581111
CTCGATT133300.010.8062071
CTCGACT124550.010.7256431
CGGGAAT69450.010.6101181
CTCGTTT112050.010.5644831
CCGAAAT74050.010.4646171
CGACAAT90600.010.3895751
CTCGGAT124750.010.3654731
CGCGAAT28100.010.3201081
CTGGAAT229400.010.2789561
TTACTCG73000.010.1478913
CGCAAAT60050.010.1334591
CTCGGTT111000.09.6793511
CTCAGAT207400.09.6730741
CTGGGAT174900.09.5406521
CTTGAAT218500.09.4210041
CTTTGAT341400.09.3994191
TTTACTC113150.09.3589462
Produced by FastQC (version 0.11.2)