FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 kx0-2.3410000000ca1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 kx0-2.3410000000ca1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24962007
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG2479090.9931453027795402TruSeq Adapter, Index 8 (100% over 49bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1036210.41511485835253553TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG612050.042.7598271
AGAGCAC612550.042.605699
CGTATGC441000.040.20240844-45
TATGCCG441750.040.0653446-47
ATCGGAA656250.039.885853
TCGGAAG664450.039.491934
CGGAAGA677900.038.638265
CTCGTAT448450.038.58352742-43
CGTCTGA483200.038.1114516-17
CACGTCT493750.037.30673614-15
GATCGGA705000.037.1482162
CACACGT496700.037.10447312-13
TCACACT509550.035.9006430-31
AGTCACA529650.034.5952228-29
CCAGTCA545850.033.5259226-27
AGCACAC559500.033.08357210-11
CCGTCTT535100.033.0025250-51
ACACTTG560850.032.7019832-33
AAGAGCA806450.032.5442628
TGCCGTC551400.031.96019448-49
Produced by FastQC (version 0.11.2)