FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 k48-3.3410000000ccaf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 k48-3.3410000000ccaf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16818790
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67900.022.2813946-47
CGCGTAT35050.021.8397731
CGTATGC69550.021.72096344-45
CACACGT76350.020.5874912-13
CTCGTAT76600.019.62974742-43
TAGCTTA78450.019.44155334-35
ACATGCG46900.019.0350484
CACGTCT86650.018.46893714-15
CATGCGT47300.018.3719965
GCGTATT43000.017.6701342
TAAGTCG44050.017.0325689
TCACTAG90950.016.71737530-31
CGCGTGT22750.016.2817673
CGTCTGA96500.016.23929416-17
ATGCGTG61800.015.8287886
AGTCACT96850.015.74746628-29
AGCACAC110100.014.53520710-11
ACTAGCT107250.014.44273732-33
TCCGTAC41800.013.8595765
CGTGCGA62050.013.5456699
Produced by FastQC (version 0.11.2)