FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l07n01 k12-2.3410000000cb36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l07n01 k12-2.3410000000cb36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22782943
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC521990.22911438614405524TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG172200.023.91624546-47
CGTATGC181150.022.78982244-45
CACACGT187900.022.72015412-13
CTCGTAT177250.022.39490742-43
ACTGACC189150.022.30733132-33
CGTCTGA193100.022.10822516-17
ATCGGAA280500.021.8217622
AGAGCAC290500.021.478358
GAGCACA299300.021.116559
AGTCACT206450.020.60945128-29
TCGGAAG321550.019.0949213
GATCGGA334950.018.5787221
CACGTCT234800.018.39420714-15
CGGAAGA338250.018.3340724
AGCACAC239550.017.90018510-11
TCGTATG176650.017.4012242-43
ACGTCTG183200.017.21151214-15
CCAGTCA250050.017.00455326-27
ACCAATC249050.016.18797536-37
TCTGAAC268700.016.05480818-19
Produced by FastQC (version 0.11.2)