##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 mx48-1.3420000000cc18.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12373852 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.35803806284413 33.0 33.0 33.0 33.0 33.0 2 32.25493459918545 33.0 33.0 33.0 33.0 33.0 3 32.22120799569932 33.0 33.0 33.0 33.0 33.0 4 36.059722954501154 37.0 37.0 37.0 37.0 37.0 5 36.062853265094816 37.0 37.0 37.0 37.0 37.0 6 36.17332961473921 37.0 37.0 37.0 37.0 37.0 7 36.1201427009148 37.0 37.0 37.0 37.0 37.0 8 36.17560125981788 37.0 37.0 37.0 37.0 37.0 9 36.06927212318363 37.0 37.0 37.0 37.0 37.0 10-11 36.06426701240648 37.0 37.0 37.0 37.0 37.0 12-13 36.05247973711016 37.0 37.0 37.0 37.0 37.0 14-15 38.36352762260289 40.0 40.0 40.0 37.0 40.0 16-17 38.33280752024511 40.0 40.0 40.0 37.0 40.0 18-19 38.317951637048836 40.0 40.0 40.0 37.0 40.0 20-21 38.286978743563445 40.0 40.0 40.0 37.0 40.0 22-23 38.4146852168589 40.0 40.0 40.0 37.0 40.0 24-25 38.287464445186515 40.0 40.0 40.0 37.0 40.0 26-27 38.252755487943446 40.0 40.0 40.0 37.0 40.0 28-29 38.14376941796297 40.0 38.5 40.0 37.0 40.0 30-31 38.08843822441064 40.0 37.0 40.0 37.0 40.0 32-33 38.0001783195726 40.0 37.0 40.0 37.0 40.0 34-35 37.93711905556976 40.0 37.0 40.0 37.0 40.0 36-37 37.8750924530211 40.0 37.0 40.0 37.0 40.0 38-39 37.8004948661096 40.0 37.0 40.0 33.0 40.0 40-41 37.72272413634816 40.0 37.0 40.0 33.0 40.0 42-43 37.59753482585698 40.0 37.0 40.0 33.0 40.0 44-45 37.553105977023165 40.0 37.0 40.0 33.0 40.0 46-47 37.33946829976631 40.0 37.0 40.0 33.0 40.0 48-49 37.23924902285885 40.0 37.0 40.0 33.0 40.0 50-51 35.79317378290932 38.5 35.0 38.5 30.0 40.0 52-53 36.15118396437908 37.0 37.0 40.0 33.0 40.0 54-55 36.827622190729286 37.0 37.0 40.0 33.0 40.0 56-57 36.852211744572344 37.0 37.0 40.0 33.0 40.0 58-59 36.68981037594437 37.0 37.0 40.0 33.0 40.0 60-61 36.644415578915925 37.0 37.0 40.0 33.0 40.0 62-63 36.506598187856135 37.0 37.0 40.0 33.0 40.0 64-65 36.30276085409782 37.0 37.0 40.0 33.0 40.0 66-67 36.01017544900327 37.0 37.0 40.0 33.0 40.0 68-69 35.72638536488072 37.0 37.0 37.0 33.0 40.0 70-71 35.48404538861463 37.0 37.0 37.0 33.0 40.0 72-73 35.26237217804125 37.0 37.0 37.0 33.0 40.0 74-75 35.078562358754574 37.0 37.0 37.0 33.0 38.5 76-77 34.88327947513838 37.0 37.0 37.0 33.0 37.0 78-79 34.72009839781501 37.0 35.0 37.0 33.0 37.0 80-81 34.604159036329186 37.0 33.0 37.0 33.0 37.0 82-83 34.51028503492688 37.0 33.0 37.0 30.0 37.0 84-85 34.4099766588448 37.0 33.0 37.0 27.0 37.0 86-87 34.32395918425402 37.0 33.0 37.0 27.0 37.0 88-89 34.24034876124266 37.0 33.0 37.0 27.0 37.0 90-91 34.14725224610736 37.0 33.0 37.0 27.0 37.0 92-93 34.0964784450307 37.0 33.0 37.0 27.0 37.0 94-95 34.03266557576411 37.0 33.0 37.0 27.0 37.0 96-97 33.973290734364696 37.0 33.0 37.0 27.0 37.0 98-99 33.90111058383437 37.0 33.0 37.0 27.0 37.0 100-101 32.22350509768502 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.047245621384938374 2306 2 0.14345152941102413 2306 3 0.17214109246678078 2306 4 0.24360716603215593 2306 5 0.23566361923020906 2306 6 0.2672502583874987 2306 7 0.322784361050374 2306 8 0.2094487202579316 2306 9 0.2534936759746955 2306 10-11 0.28148988719680546 2306 12-13 0.2790638536189576 2306 14-15 0.5426664252452795 2306 16-17 0.46850830949581734 2306 18-19 0.5376559362204532 2306 20-21 0.5686209716985218 2306 22-23 0.48841807008559357 2306 24-25 0.545490113336804 2306 26-27 0.5404296632903325 2306 28-29 0.6076913821232068 2306 30-31 0.6116386216788712 2306 32-33 0.5698126440510123 2306 34-35 0.6110006718544412 2306 36-37 0.6008702829714494 2306 38-39 0.6508046813785384 2306 40-41 0.6345505499686723 2306 42-43 0.6468173383617852 2306 44-45 0.6724608143415409 2306 46-47 0.6664848311408562 2306 48-49 0.6196317042174186 2306 50-51 0.4906460190504234 2306 52-53 0.5460878120577561 2306 54-55 0.6402588093549113 2306 56-57 0.565330437596316 2306 58-59 0.6206541050325001 2306 60-61 0.5734712847025563 2306 62-63 0.6340632001709992 2306 64-65 0.6736779809564055 2306 66-67 0.7128843271526861 2306 68-69 0.7109404511826725 2306 70-71 0.7302733488705044 2306 72-73 0.7244460773656414 2306 74-75 0.7545102723786172 2306 76-77 0.6541366679306151 2306 78-79 0.6851988763920573 2306 80-81 0.7515273144390306 2306 82-83 0.7105717143426418 2306 84-85 0.7258038002957292 2306 86-87 0.7079727487277907 2306 88-89 0.719129024487664 2306 90-91 0.8094127091314647 2306 92-93 0.7746304983987784 2306 94-95 0.7709503051976725 2306 96-97 0.72191151401843 2306 98-99 0.7405476848416654 2306 100-101 0.6359710235012201 2307 1 -0.10525883355818166 2307 2 0.10493017973558949 2307 3 0.15292785133201647 2307 4 0.21496716057562537 2307 5 0.24278630386999112 2307 6 0.2809402096881328 2307 7 0.28413986618458864 2307 8 0.24961764477646398 2307 9 0.2650391180529681 2307 10-11 0.2585580033832571 2307 12-13 0.28296489215376397 2307 14-15 0.5336367626993805 2307 16-17 0.519587493615397 2307 18-19 0.5429387715949261 2307 20-21 0.557924008274405 2307 22-23 0.5852208560693057 2307 24-25 0.6085393623947368 2307 26-27 0.612979200982771 2307 28-29 0.5866222351658976 2307 30-31 0.6210832876625645 2307 32-33 0.6191972757178377 2307 34-35 0.5971558354162383 2307 36-37 0.5977586797402807 2307 38-39 0.6387503739349611 2307 40-41 0.6499615485963517 2307 42-43 0.6864068378087254 2307 44-45 0.6779872449999615 2307 46-47 0.6491857202843718 2307 48-49 0.6361251780924491 2307 50-51 0.6274962014203638 2307 52-53 0.6178896884463185 2307 54-55 0.5985579192784769 2307 56-57 0.6414089764475364 2307 58-59 0.6419912482651853 2307 60-61 0.6565389143203859 2307 62-63 0.6772828083681972 2307 64-65 0.6455597151350716 2307 66-67 0.7018408364799456 2307 68-69 0.6546125868571551 2307 70-71 0.6231708567249044 2307 72-73 0.7266435763365848 2307 74-75 0.764400987241622 2307 76-77 0.7231468594390122 2307 78-79 0.7471768659514524 2307 80-81 0.7397706597009375 2307 82-83 0.7591056490833594 2307 84-85 0.7779640810170108 2307 86-87 0.7961788912886618 2307 88-89 0.7225541607636856 2307 90-91 0.7871745535893382 2307 92-93 0.7807851146486158 2307 94-95 0.7621869285772789 2307 96-97 0.7952764428694294 2307 98-99 0.746003835669228 2307 100-101 0.692367716451642 2201 1 0.1109035836827772 2201 2 0.20375940436670703 2201 3 0.22837442359334403 2201 4 0.27729710748283054 2201 5 0.2917595271592077 2201 6 0.28426812737219365 2201 7 0.3438291456765086 2201 8 0.23488104528665588 2201 9 0.28148292025982613 2201 10-11 0.28527351805549017 2201 12-13 0.32108550507555833 2201 14-15 0.5287996768486423 2201 16-17 0.501625934767084 2201 18-19 0.4909603401925864 2201 20-21 0.5504593832286062 2201 22-23 0.5677450155263912 2201 24-25 0.5547300611299519 2201 26-27 0.5015606247716775 2201 28-29 0.5474129016973635 2201 30-31 0.574505249576184 2201 32-33 0.6074667113156664 2201 34-35 0.6080745865152082 2201 36-37 0.6249405132743746 2201 38-39 0.6148904797484178 2201 40-41 0.5628133388118002 2201 42-43 0.6311086809384818 2201 44-45 0.5630751322320648 2201 46-47 0.574042027434551 2201 48-49 0.6059979370389215 2201 50-51 0.5547359610726303 2201 52-53 0.586704273216732 2201 54-55 0.5918745956224711 2201 56-57 0.5936035617561757 2201 58-59 0.6285821582096958 2201 60-61 0.594070587013114 2201 62-63 0.6648644682238682 2201 64-65 0.6628567138275301 2201 66-67 0.7125749741355705 2201 68-69 0.7549132231003313 2201 70-71 0.7402633136113579 2201 72-73 0.7512170719898421 2201 74-75 0.7387105419224085 2201 76-77 0.7895977667351275 2201 78-79 0.736690122886813 2201 80-81 0.6961972439540745 2201 82-83 0.6976319136729572 2201 84-85 0.7635562038919161 2201 86-87 0.764941212993385 2201 88-89 0.7236853671254835 2201 90-91 0.7659747779475197 2201 92-93 0.7292997754076467 2201 94-95 0.7593668679951975 2201 96-97 0.7893028356228484 2201 98-99 0.7525869317384135 2201 100-101 0.6773184344567511 1101 1 0.01140047626343943 1101 2 0.1552365319262563 1101 3 0.2090750501002816 1101 4 0.2640665685786274 1101 5 0.3326709136963686 1101 6 0.3269470771349319 1101 7 0.3480217621743549 1101 8 0.3181792185362369 1101 9 0.3513810259851482 1101 10-11 0.31839406354740163 1101 12-13 0.345267941972466 1101 14-15 0.5660000942815415 1101 16-17 0.5768425117322451 1101 18-19 0.58222309246284 1101 20-21 0.5846070747829302 1101 22-23 0.5901382441011194 1101 24-25 0.5820822130129599 1101 26-27 0.5972977599046843 1101 28-29 0.6227849784443507 1101 30-31 0.6464528397512055 1101 32-33 0.6252395247758784 1101 34-35 0.6440110640483638 1101 36-37 0.6148957595206781 1101 38-39 0.6604372323546386 1101 40-41 0.6598538747914162 1101 42-43 0.6562065400040282 1101 44-45 0.6282578096675877 1101 46-47 0.6798110489272418 1101 48-49 0.6878349501231469 1101 50-51 0.5469250798354963 1101 52-53 0.6639534901710462 1101 54-55 0.7064900701644987 1101 56-57 0.6526712917482413 1101 58-59 0.6475915718769016 1101 60-61 0.6435895280845898 1101 62-63 0.6659604703301767 1101 64-65 0.6760945592716538 1101 66-67 0.71811300056509 1101 68-69 0.7148916295296104 1101 70-71 0.7138554510648589 1101 72-73 0.7452609749187928 1101 74-75 0.7798357468187618 1101 76-77 0.7831275440669856 1101 78-79 0.7734066826626034 1101 80-81 0.7462891289729612 1101 82-83 0.7939182852134152 1101 84-85 0.7730385582038011 1101 86-87 0.7645479198282814 1101 88-89 0.7183997049247637 1101 90-91 0.7665251969163478 1101 92-93 0.78391508439109 1101 94-95 0.7686218256092374 1101 96-97 0.7134350544878103 1101 98-99 0.724850517798906 1101 100-101 0.6655048574864892 2304 1 -0.0615753838644153 2304 2 0.07793358991185073 2304 3 0.15773684866643123 2304 4 0.18551273372479926 2304 5 0.20622859561489548 2304 6 0.23441002662737276 2304 7 0.2202022614435677 2304 8 0.22771583511575955 2304 9 0.22261695971931772 2304 10-11 0.2637126319387022 2304 12-13 0.2672088824987142 2304 14-15 0.4186666583561376 2304 16-17 0.4776110318665374 2304 18-19 0.46180603471679404 2304 20-21 0.4722023823989332 2304 22-23 0.4804847846089828 2304 24-25 0.504342272654533 2304 26-27 0.45947769175289466 2304 28-29 0.49279941842562636 2304 30-31 0.4838078614091117 2304 32-33 0.4764968645776264 2304 34-35 0.4785544332413565 2304 36-37 0.5128640390861392 2304 38-39 0.5370155203488949 2304 40-41 0.515965996628843 2304 42-43 0.5620095139910575 2304 44-45 0.5858776982593312 2304 46-47 0.5796396676387943 2304 48-49 0.5674141321090573 2304 50-51 0.5524592698623039 2304 52-53 0.5384233510223666 2304 54-55 0.5927048005405808 2304 56-57 0.5727478913752364 2304 58-59 0.5410151638786829 2304 60-61 0.5781113112691045 2304 62-63 0.6138686490829173 2304 64-65 0.5962311299993743 2304 66-67 0.6364702055519089 2304 68-69 0.6494480623767842 2304 70-71 0.6827808856807778 2304 72-73 0.6451323709916466 2304 74-75 0.66333293492481 2304 76-77 0.6847794719088114 2304 78-79 0.6573399031722573 2304 80-81 0.6466751849993244 2304 82-83 0.6617483406371463 2304 84-85 0.646291327230692 2304 86-87 0.6237156837802686 2304 88-89 0.6475568551234474 2304 90-91 0.6536119989992102 2304 92-93 0.6967489454820708 2304 94-95 0.6951027067919711 2304 96-97 0.6866274618980768 2304 98-99 0.7129090931985118 2304 100-101 0.7870805331638806 2202 1 0.12361647757303729 2202 2 0.10872942862968671 2202 3 0.12633163217526544 2202 4 0.16162919221729055 2202 5 0.16650821729787424 2202 6 0.23765886925541935 2202 7 0.17212761078889116 2202 8 0.1974430115811785 2202 9 0.1707706181375741 2202 10-11 0.18493104418065798 2202 12-13 0.18797288685725988 2202 14-15 0.3447713991914796 2202 16-17 0.3851649214671369 2202 18-19 0.387720307002013 2202 20-21 0.3628741736830179 2202 22-23 0.40728191024265215 2202 24-25 0.4560644821683937 2202 26-27 0.3921262970954089 2202 28-29 0.4196349333928211 2202 30-31 0.46751150886664306 2202 32-33 0.43750297504378466 2202 34-35 0.4308231409339669 2202 36-37 0.4183949401453617 2202 38-39 0.5000389452925234 2202 40-41 0.5109199061840357 2202 42-43 0.4978070459878339 2202 44-45 0.5133115110788751 2202 46-47 0.4745420993685414 2202 48-49 0.5293801368373607 2202 50-51 0.4921377602370782 2202 52-53 0.4713713526373269 2202 54-55 0.5114170407480287 2202 56-57 0.49691796118887765 2202 58-59 0.5498265261677773 2202 60-61 0.5885142186785188 2202 62-63 0.6013801477045106 2202 64-65 0.5975089625641843 2202 66-67 0.5968130703986887 2202 68-69 0.6223466829474873 2202 70-71 0.6009435403511745 2202 72-73 0.6451929320874257 2202 74-75 0.6222757483474055 2202 76-77 0.5884306545398843 2202 78-79 0.64761713245057 2202 80-81 0.6301935313577758 2202 82-83 0.5941647563715193 2202 84-85 0.6546376666071865 2202 86-87 0.6274410047314305 2202 88-89 0.6383376754524264 2202 90-91 0.6526538868222502 2202 92-93 0.6296071335794338 2202 94-95 0.6865611069038238 2202 96-97 0.6705772516775923 2202 98-99 0.6789288410865026 2202 100-101 0.778643235920768 1102 1 0.0742034488668466 1102 2 0.08501500119614036 1102 3 0.11676915705107405 1102 4 0.16417798659266936 1102 5 0.1798751953633797 1102 6 0.20089582195183198 1102 7 0.21255300239631225 1102 8 0.17334318671206717 1102 9 0.2096447238140371 1102 10-11 0.17196111461617392 1102 12-13 0.2073022129538984 1102 14-15 0.3408700125583053 1102 16-17 0.36220752657239075 1102 18-19 0.3942740704134451 1102 20-21 0.35378761982055806 1102 22-23 0.35482021214286874 1102 24-25 0.36292617776593517 1102 26-27 0.3940401996315117 1102 28-29 0.38857684201458653 1102 30-31 0.4124150800094313 1102 32-33 0.4095123980710298 1102 34-35 0.4486769521225895 1102 36-37 0.36724908262043243 1102 38-39 0.41147432267106154 1102 40-41 0.3934010826757728 1102 42-43 0.48294917465052123 1102 44-45 0.43183097083434774 1102 46-47 0.44926595999468333 1102 48-49 0.46156882466934945 1102 50-51 0.41900537780792746 1102 52-53 0.4344358330740974 1102 54-55 0.5065305265565385 1102 56-57 0.5045795402236237 1102 58-59 0.46575160382199954 1102 60-61 0.519071053592306 1102 62-63 0.5334356432670759 1102 64-65 0.5273746051882071 1102 66-67 0.5510419510180142 1102 68-69 0.5240649414181604 1102 70-71 0.5433378838240586 1102 72-73 0.554494392067582 1102 74-75 0.5967671089650821 1102 76-77 0.6134466649804864 1102 78-79 0.6200985669860515 1102 80-81 0.5828152947660783 1102 82-83 0.5806792085147023 1102 84-85 0.5935679684740407 1102 86-87 0.6091583457132828 1102 88-89 0.6199051425498965 1102 90-91 0.6081562002234051 1102 92-93 0.5825909401623619 1102 94-95 0.6384023792523124 1102 96-97 0.7184396200133776 1102 98-99 0.6471616507474565 1102 100-101 0.5730875283192489 2305 1 0.06903420074255706 2305 2 0.020599253268613893 2305 3 0.059365185686829136 2305 4 0.06316230319597338 2305 5 0.09305323668840515 2305 6 0.12406151235865082 2305 7 0.11097777369712247 2305 8 0.14980114716993143 2305 9 0.09971161288514452 2305 10-11 0.13592491796702433 2305 12-13 0.1085234639245698 2305 14-15 0.22768000961701063 2305 16-17 0.22726603982108173 2305 18-19 0.28028533020979296 2305 20-21 0.28206165591925725 2305 22-23 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1213 68-69 -1.0755984577593125 1213 70-71 -1.1388895720748664 1213 72-73 -0.889990030567887 1213 74-75 -0.8940112370450137 1213 76-77 -0.8795211178579478 1213 78-79 -0.8004606515633839 1213 80-81 -0.8491796544443062 1213 82-83 -0.8742788892571767 1213 84-85 -0.9252292870397412 1213 86-87 -0.923601492381728 1213 88-89 -0.9404006683009669 1213 90-91 -0.9238994032345573 1213 92-93 -0.9121483777889452 1213 94-95 -0.9804839341419367 1213 96-97 -1.0576509570971595 1213 98-99 -0.9493754883449625 1213 100-101 -0.936845688732383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11307.0 3 1129.0 4 779.0 5 562.0 6 562.0 7 707.0 8 1433.0 9 7255.0 10 39412.0 11 63879.0 12 37749.0 13 24319.0 14 20246.0 15 18854.0 16 18773.0 17 20003.0 18 21810.0 19 23591.0 20 26008.0 21 28646.0 22 32092.0 23 35855.0 24 40444.0 25 46311.0 26 54811.0 27 77325.0 28 81016.0 29 89970.0 30 111959.0 31 144111.0 32 191171.0 33 263662.0 34 376349.0 35 607780.0 36 1396603.0 37 4214698.0 38 4236925.0 39 5746.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.396876888466338 13.276170288609865 14.487969746010435 49.838983076913365 2 23.66650083035512 19.39626032237964 36.378214272331064 20.559024574934178 3 24.85791283226656 23.680193628369125 25.830870780487064 25.631022758877254 4 26.821256107465164 27.85881494414956 18.67605096640524 26.643877981980037 5 29.34655939834506 29.726980908745865 21.1975029364529 19.72895675645617 6 22.732173505723157 32.95311083595689 22.042393075821888 22.27232258249806 7 20.275659002181694 16.316427518619147 40.47087384060648 22.937039638592683 8 21.345350767009815 21.984170992374317 27.000991375983592 29.669486864632276 9 22.709161638525526 20.965807561666352 29.97737437601571 26.347656423792415 10-11 25.45002950784442 29.07474052084004 20.852034811855695 24.62319515945984 12-13 23.89371626014774 22.84729044992617 27.461366155323564 25.797627134602525 14-15 24.01426377998667 24.581778651881887 26.280111091392243 25.123846476739196 16-17 25.42048868716464 24.608295083790193 25.256340664528366 24.7148755645168 18-19 25.301685191836633 24.94411333991483 25.435014462819026 24.31918700542951 20-21 24.871125910055696 25.432597342269258 25.47255384505045 24.223722902624605 22-23 25.533181243260273 24.899152918507497 25.152086026392713 24.41557981183952 24-25 24.709042019029248 25.82634300275054 25.04479801772287 24.41981696049734 26-27 24.26569712616718 26.17717268803183 25.159569487894323 24.397560697906666 28-29 25.00343511357724 25.556144483547467 24.839366968044946 24.60105343483035 30-31 25.42451428324594 24.796695519568207 25.178422270516215 24.600367926669637 32-33 24.755807644627488 25.44461832299912 25.272966790939922 24.526607241433474 34-35 24.812214107594947 25.706574433996455 24.719276951188736 24.761934507219866 36-37 24.01669671139309 25.3980541892609 25.541229893378038 25.04401920596797 38-39 24.062729462372936 24.978634927749177 25.862262677570424 25.096372932307464 40-41 25.537637509305096 24.79482449516734 24.860405863842086 24.807132131685478 42-43 25.3681766056438 24.836924810040326 25.405662736503736 24.389235847812134 44-45 25.533464262680848 24.74743906889651 25.295723918720224 24.423372749702416 46-47 25.15165199130726 24.729035962634324 24.97627702461252 25.143035021445893 48-49 24.20486039422388 24.915046332843488 24.8777897877474 26.00230348518523 50-51 24.709805379391096 24.896365785481233 25.47996251388478 24.913866321242896 52-53 24.45405023798469 25.316189018522202 25.69896447879003 24.53079626470308 54-55 24.029148600288543 25.275376748304367 25.34613740350305 25.349337247904042 56-57 23.825590603341297 25.00787943085246 26.597977096959173 24.56855286884707 58-59 24.19882714135822 25.777505407348166 25.48503307273988 24.538634378553734 60-61 24.11058626000386 26.16897344978725 25.095571776624258 24.624868513584634 62-63 23.963489530204836 26.30515027759103 25.226675645124047 24.504684547080092 64-65 24.085762155980145 26.378481463044317 24.80791080561011 24.72784557536543 66-67 24.0071758472218 26.32519283258505 24.875440898776606 24.792190421416546 68-69 24.1428062078059 26.256636267250087 25.01158408263695 24.58897344230706 70-71 24.300667409630908 26.113492609040428 24.848804133553454 24.737035847775214 72-73 24.166861414705863 26.10171285485952 25.15059256271804 24.58083316771658 74-75 24.48719444737551 25.941879386254364 25.036412354324778 24.534513812045347 76-77 24.740421528069618 25.87659101380343 24.79954784802706 24.583439610099898 78-79 24.356690994888208 25.835590138950792 24.97601393539354 24.831704930767458 80-81 24.543409519690577 25.900543752015153 24.838597393667783 24.717449334626483 82-83 24.53443723715588 25.880538160396032 24.741637680128605 24.84338692231948 84-85 24.450417873676336 25.72764197296339 24.854439641019695 24.967500512340578 86-87 24.456473694506183 26.043119595106205 24.90326644779896 24.597140262588653 88-89 24.643605509527738 25.880918750933112 24.84408107711162 24.63139466242753 90-91 24.716572484208253 25.640295173649104 24.84989253382633 24.79323980831631 92-93 24.645114830180553 25.857669078258695 25.087663211458715 24.409552880102034 94-95 24.588628728446576 25.741627707866527 24.909847683241207 24.75989588044569 96-97 24.375198632180776 25.866238581279156 24.923738997152643 24.834823789387425 98-99 24.64599851113676 25.623732031803144 25.037604902010006 24.692664555050094 100-101 24.75779228538888 25.735454086420585 24.874018493118378 24.63273513507216 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 9976.0 1 5312.5 2 593.5 3 512.5 4 441.5 5 413.0 6 384.5 7 325.0 8 321.5 9 329.5 10 367.5 11 426.5 12 427.0 13 460.5 14 542.0 15 628.5 16 732.0 17 918.0 18 1173.5 19 1479.0 20 1944.5 21 2673.0 22 3805.0 23 5352.0 24 7416.5 25 10527.5 26 14864.5 27 20198.0 28 27388.0 29 36619.5 30 47447.5 31 60638.0 32 76337.0 33 95581.0 34 119698.0 35 150750.0 36 187598.5 37 233263.0 38 282231.0 39 329598.0 40 380188.5 41 428506.5 42 468727.0 43 502378.0 44 526373.5 45 526767.0 46 511821.0 47 506600.5 48 483712.5 49 450344.0 50 441868.5 51 417449.0 52 385192.0 53 367084.0 54 352563.5 55 348893.5 56 353126.5 57 350800.0 58 327190.5 59 283868.5 60 256733.5 61 238183.0 62 213228.5 63 193577.5 64 166290.0 65 148256.5 66 147476.0 67 144763.0 68 131514.5 69 111987.0 70 93890.5 71 81112.0 72 65699.0 73 51191.5 74 47854.0 75 40678.0 76 26539.5 77 17053.0 78 13091.5 79 12340.5 80 10259.0 81 6866.5 82 3269.5 83 971.0 84 618.5 85 470.5 86 386.0 87 286.0 88 231.0 89 197.5 90 164.5 91 137.5 92 103.0 93 90.0 94 66.5 95 41.5 96 32.0 97 21.0 98 10.5 99 5.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15556998742186345 2 0.1478520997341814 3 0.15729944078852728 4 0.1526606266181299 5 0.1588915076727926 6 0.10592497792926568 7 0.11092746220012976 8 0.10711296692412355 9 0.0950552827042056 10-11 0.08235511464012985 12-13 0.09088519888552085 14-15 0.13592372043887385 16-17 0.14491041269929525 18-19 0.13314770533864476 20-21 0.16501328769731527 22-23 0.1455973451112879 24-25 0.1418919508654217 26-27 0.132400161243241 28-29 0.1307757681278231 30-31 0.09379455968925442 32-33 0.10549261458759972 34-35 0.12092434918406976 36-37 0.126153117073002 38-39 0.12510655533943674 40-41 0.15947742061243336 42-43 0.1396654816947867 44-45 0.12964435003748226 46-47 0.16422937659186485 48-49 0.18589199224299757 50-51 0.16118262930573277 52-53 0.10993343059218746 54-55 0.11213969586835208 56-57 0.11426918634552927 58-59 0.11354992770238402 60-61 0.11868171689785847 62-63 0.10030425448760821 64-65 0.117623032827611 66-67 0.13387504553957813 68-69 0.13235975345430023 70-71 0.1196110960434956 72-73 0.1293372508415326 74-75 0.16614470578765608 76-77 0.1941351811869093 78-79 0.16846815365174886 80-81 0.14129795636799275 82-83 0.12549851089216194 84-85 0.132129429057338 86-87 0.12566418282681902 88-89 0.12919986435913408 90-91 0.14442551923200633 92-93 0.11008293941126822 94-95 0.11487126240074634 96-97 0.11576023375744271 98-99 0.11927167061639335 100-101 0.1123578979286321 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.2373852E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.03278381996179 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34843348170773 41.02002751648101 2 13.774644467549876 14.610154844888104 3 3.85031284670426 6.125784265154661 4 1.6831818945344013 3.5705528617006648 5 0.8964814490509577 2.3771453443062764 6 0.4893609188767385 1.557130309243676 7 0.44236159251600765 1.6421766694308824 8 0.23345657783212606 0.9904681778815381 9 0.1721449717501789 0.82163943652686 >10 0.9437874493208641 9.104146932830735 >50 0.08242427577168754 3.0993789543125247 >100 0.07035741972026126 7.43011573520162 >500 0.008476307663878524 3.0716620290312493 >1k 0.00443680094492633 3.925774426605743 >5k 9.37582774746513E-5 0.27931066583744624 >10k+ 4.578777868310744E-5 0.3745318305671584 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG 21399 0.17293725510859512 Illumina Single End PCR Primer 1 (100% over 50bp) AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 14817 0.11974444174700004 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.12619352486194274 3.2326231152595005E-5 8.081557788148751E-6 2 0.12634707445991758 3.2326231152595005E-5 3.2326231152595005E-5 3 0.12656527652019758 3.2326231152595005E-5 3.2326231152595005E-5 4 0.12738959541458877 3.2326231152595005E-5 3.2326231152595005E-5 5 0.1275269818969873 3.2326231152595005E-5 3.2326231152595005E-5 6 0.1280926909421577 4.0407788940743756E-5 3.2326231152595005E-5 7 0.12825432209792068 4.0407788940743756E-5 4.0407788940743756E-5 8 0.12855333973608218 4.0407788940743756E-5 4.0407788940743756E-5 9 0.12883619425866738 4.0407788940743756E-5 4.0407788940743756E-5 10-11 0.12951504511287187 6.0611683411115635E-5 4.848934672889251E-5 12-13 0.13028279310274601 7.677479898741314E-5 5.657090451704126E-5 14-15 0.13115964212276016 9.293791456371065E-5 6.465246230519001E-5 16-17 0.13203245036388023 1.1718258792815689E-4 6.465246230519001E-5 18-19 0.13282848380601286 1.2930492461038002E-4 6.465246230519001E-5 20-21 0.1340245543586589 1.495088190807519E-4 6.465246230519001E-5 22-23 0.13463067119277006 2.0203894470371877E-4 6.869324119926438E-5 24-25 0.13527315503692788 2.4648751253853693E-4 7.677479898741314E-5 26-27 0.1358024970720516 2.5860984922076004E-4 8.081557788148751E-5 28-29 0.1363722468961161 2.5860984922076004E-4 8.485635677556188E-5 30-31 0.13699856762469764 2.9093608037335505E-4 8.889713566963626E-5 32-33 0.1376046844588088 2.990176381615038E-4 9.697869345778502E-5 34-35 0.13819059739844958 3.070991959496526E-4 1.0910103014000815E-4 36-37 0.1387199394335733 3.313438693140988E-4 1.1314180903408252E-4 38-39 0.13924119991090889 3.515477637844707E-4 1.2122336682223127E-4 40-41 0.13995641777516007 3.5558854267854506E-4 1.2526414571630565E-4 42-43 0.14063930940825864 3.636701004666938E-4 1.4546804018667752E-4 44-45 0.14129795636799275 3.9195555272521443E-4 1.5354959797482626E-4 46-47 0.1420374189056084 4.0407788940743754E-4 1.5354959797482626E-4 48-49 0.14290618636783436 4.121594471955863E-4 1.7779427133927253E-4 50-51 0.14394870732250556 4.485264572422557E-4 1.8587582912742127E-4 52-53 0.1449669836038123 4.646895728185532E-4 1.9395738691557003E-4 54-55 0.1462923590810687 4.7277113060670197E-4 2.0203894470371877E-4 56-57 0.14765006078947768 4.808526883948507E-4 2.0607972359779314E-4 58-59 0.14911686352802667 5.091381406533713E-4 2.1012050249186753E-4 60-61 0.1506604410655631 5.253012562296688E-4 2.1012050249186753E-4 62-63 0.1525070770201551 5.333828140178176E-4 2.1012050249186753E-4 64-65 0.15447089556267524 5.414643718059663E-4 2.1012050249186753E-4 66-67 0.1567620171956154 5.455051507000407E-4 2.1012050249186753E-4 68-69 0.15892787468283925 5.657090451704126E-4 2.2224283917409067E-4 70-71 0.16137254591375427 5.657090451704126E-4 2.2628361806816504E-4 72-73 0.1645162718933441 5.818721607467101E-4 2.303243969622394E-4 74-75 0.16774889500860363 5.980352763230076E-4 2.3436517585631377E-4 76-77 0.17134518822432981 6.303615074756026E-4 2.4244673364446254E-4 78-79 0.17564861774651902 6.303615074756026E-4 2.5860984922076004E-4 80-81 0.18041269606263272 6.465246230519001E-4 2.7073218590298315E-4 82-83 0.1860940311877013 6.546061808400488E-4 2.9093608037335505E-4 84-85 0.1933149030714122 6.667285175222719E-4 2.9093608037335505E-4 86-87 0.20190963977910842 6.707692964163463E-4 3.0305841705557815E-4 88-89 0.21224595219015063 6.950139697807925E-4 3.151807537378013E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCGTC 11160 0.0 43.833126 9 AAGAGCG 14525 0.0 35.59696 7 ATCGGAA 15830 0.0 34.68723 2 TCGGAAG 15995 0.0 33.9131 3 CCGTATC 8790 0.0 33.624645 48-49 CGCCGTA 9440 0.0 31.788486 46-47 AGAGCGT 15630 0.0 31.593544 8 TCGTGTA 8630 0.0 31.407373 14-15 GTAGGGA 9035 0.0 30.9461 18-19 GATCGGG 7770 0.0 30.32193 1 GATCGGA 18320 0.0 30.102526 1 TTAAAAA 28720 0.0 29.31563 56-57 ATCGGGA 8205 0.0 28.540464 2 CGGAAGA 19065 0.0 28.27774 4 CGGGAGA 7595 0.0 27.455061 4 CGCGTGT 2795 0.0 27.190914 3 GCCGTAT 8225 0.0 27.182674 46-47 TCGGGAG 7565 0.0 26.936056 3 TAGATCT 12585 0.0 26.756779 32-33 TTTAAAA 16190 0.0 25.899345 56-57 >>END_MODULE