FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 mx48-1.3420000000cc18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 mx48-1.3420000000cc18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12373852
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG213990.17293725510859512Illumina Single End PCR Primer 1 (100% over 50bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC148170.11974444174700004Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC111600.043.8331269
AAGAGCG145250.035.596967
ATCGGAA158300.034.687232
TCGGAAG159950.033.91313
CCGTATC87900.033.62464548-49
CGCCGTA94400.031.78848646-47
AGAGCGT156300.031.5935448
TCGTGTA86300.031.40737314-15
GTAGGGA90350.030.946118-19
GATCGGG77700.030.321931
GATCGGA183200.030.1025261
TTAAAAA287200.029.3156356-57
ATCGGGA82050.028.5404642
CGGAAGA190650.028.277744
CGGGAGA75950.027.4550614
CGCGTGT27950.027.1909143
GCCGTAT82250.027.18267446-47
TCGGGAG75650.026.9360563
TAGATCT125850.026.75677932-33
TTTAAAA161900.025.89934556-57
Produced by FastQC (version 0.11.2)