##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 mx24-1.3420000000ca5e.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 17765481 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47241169546718 33.0 33.0 33.0 33.0 33.0 2 32.460605766880164 33.0 33.0 33.0 33.0 33.0 3 32.454049119187935 33.0 33.0 33.0 33.0 33.0 4 36.32715860606307 37.0 37.0 37.0 37.0 37.0 5 36.316379443934 37.0 37.0 37.0 37.0 37.0 6 36.31573735605583 37.0 37.0 37.0 37.0 37.0 7 36.358079412541656 37.0 37.0 37.0 37.0 37.0 8 36.3571259342767 37.0 37.0 37.0 37.0 37.0 9 36.35324486851777 37.0 37.0 37.0 37.0 37.0 10-11 36.3357803259028 37.0 37.0 37.0 37.0 37.0 12-13 36.32037350972934 37.0 37.0 37.0 37.0 37.0 14-15 38.63520179948969 40.0 40.0 40.0 37.0 40.0 16-17 38.61586387669436 40.0 40.0 40.0 37.0 40.0 18-19 38.58534359413066 40.0 40.0 40.0 37.0 40.0 20-21 38.54505386034862 40.0 40.0 40.0 37.0 40.0 22-23 38.51402154549038 40.0 40.0 40.0 37.0 40.0 24-25 38.47359179298326 40.0 40.0 40.0 37.0 40.0 26-27 38.4279476305764 40.0 38.5 40.0 37.0 40.0 28-29 38.37685771074816 40.0 37.0 40.0 37.0 40.0 30-31 38.319166534246946 40.0 37.0 40.0 37.0 40.0 32-33 38.241405538076904 40.0 37.0 40.0 37.0 40.0 34-35 38.160417497280264 40.0 37.0 40.0 37.0 40.0 36-37 38.07000725170346 40.0 37.0 40.0 37.0 40.0 38-39 37.952594078370296 40.0 37.0 40.0 35.0 40.0 40-41 37.83413156671638 40.0 37.0 40.0 33.0 40.0 42-43 37.72327698304369 40.0 37.0 40.0 33.0 40.0 44-45 37.60234569500257 40.0 37.0 40.0 33.0 40.0 46-47 37.45142031335938 40.0 37.0 40.0 33.0 40.0 48-49 37.29287757533838 40.0 37.0 40.0 33.0 40.0 50-51 35.83350726614157 38.5 35.0 38.5 30.0 38.5 52-53 36.18075353546577 37.0 37.0 38.5 33.0 40.0 54-55 36.85616905053119 37.0 37.0 40.0 33.0 40.0 56-57 36.807289259435194 37.0 37.0 40.0 33.0 40.0 58-59 36.6555336160051 37.0 37.0 40.0 33.0 40.0 60-61 36.476485578971946 37.0 37.0 40.0 33.0 40.0 62-63 36.293490984004315 37.0 37.0 40.0 33.0 40.0 64-65 36.105333033200736 37.0 37.0 40.0 33.0 40.0 66-67 35.87867204383602 37.0 37.0 37.0 33.0 40.0 68-69 35.67494629050573 37.0 37.0 37.0 33.0 40.0 70-71 35.48284645374927 37.0 37.0 37.0 33.0 40.0 72-73 35.27354044058812 37.0 37.0 37.0 33.0 40.0 74-75 35.09034455639 37.0 37.0 37.0 33.0 37.0 76-77 34.91154016601071 37.0 33.0 37.0 33.0 37.0 78-79 34.76424511106679 37.0 33.0 37.0 33.0 37.0 80-81 34.6513209802763 37.0 33.0 37.0 33.0 37.0 82-83 34.55807706529308 37.0 33.0 37.0 30.0 37.0 84-85 34.45822829114506 37.0 33.0 37.0 27.0 37.0 86-87 34.37205474481665 37.0 33.0 37.0 27.0 37.0 88-89 34.2878695769622 37.0 33.0 37.0 27.0 37.0 90-91 34.18410635771697 37.0 33.0 37.0 27.0 37.0 92-93 34.12523257884208 37.0 33.0 37.0 27.0 37.0 94-95 34.05115991511853 37.0 33.0 37.0 27.0 37.0 96-97 33.98142301916847 37.0 33.0 37.0 27.0 37.0 98-99 33.899953088801816 37.0 33.0 37.0 27.0 37.0 100-101 32.12615234003515 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1290821058060132 2306 2 0.23408914435822226 2306 3 0.2621274769049009 2306 4 0.34979622823489365 2306 5 0.3358852412777935 2306 6 0.3934922433550483 2306 7 0.3436104296333369 2306 8 0.3295521253494442 2306 9 0.340906057772294 2306 10-11 0.36618930904686664 2306 12-13 0.36001867705758883 2306 14-15 0.6429236741218816 2306 16-17 0.582419208328055 2306 18-19 0.6400798088904907 2306 20-21 0.6652702183702459 2306 22-23 0.6563448988455534 2306 24-25 0.6257689764899652 2306 26-27 0.6770930573274825 2306 28-29 0.7225824228503441 2306 30-31 0.7016450697462986 2306 32-33 0.6447882990800835 2306 34-35 0.7169021441236083 2306 36-37 0.729520505602224 2306 38-39 0.7189123099060524 2306 40-41 0.7379361670706288 2306 42-43 0.7303227663760481 2306 44-45 0.7433980162624749 2306 46-47 0.7072052708567966 2306 48-49 0.7546239897213454 2306 50-51 0.6402813799113432 2306 52-53 0.7191160220465136 2306 54-55 0.7802953896937979 2306 56-57 0.6924529452653161 2306 58-59 0.7388677747106271 2306 60-61 0.7699129230396196 2306 62-63 0.7276711094611414 2306 64-65 0.7550092953675431 2306 66-67 0.769357135236838 2306 68-69 0.7754777774025357 2306 70-71 0.7137731584341864 2306 72-73 0.8105879554404041 2306 74-75 0.8354068544452389 2306 76-77 0.7136656902615286 2306 78-79 0.7791379658906692 2306 80-81 0.8182692564589189 2306 82-83 0.8023056723467406 2306 84-85 0.8031871107200246 2306 86-87 0.8186494686785366 2306 88-89 0.8439513811784849 2306 90-91 0.8702757360043734 2306 92-93 0.8984481798994892 2306 94-95 0.8765765717439677 2306 96-97 0.8482136680843482 2306 98-99 0.9054025888255239 2306 100-101 0.7397883670792282 2307 1 -0.03225561215024442 2307 2 0.11622112976363752 2307 3 0.21894341150757413 2307 4 0.2777933132853505 2307 5 0.3010152285753094 2307 6 0.3034601902862448 2307 7 0.3133999115805892 2307 8 0.3459063498085868 2307 9 0.32851522876537587 2307 10-11 0.3473988905690675 2307 12-13 0.3384828918301821 2307 14-15 0.5933389470453179 2307 16-17 0.6033460206021672 2307 18-19 0.629991909579914 2307 20-21 0.6118757611012953 2307 22-23 0.6581617952481125 2307 24-25 0.6346738159939065 2307 26-27 0.657646256899497 2307 28-29 0.6714206304415313 2307 30-31 0.7046579586926782 2307 32-33 0.7028029849897237 2307 34-35 0.7197163930160144 2307 36-37 0.6703805763416497 2307 38-39 0.7129018492756458 2307 40-41 0.7349306718081792 2307 42-43 0.7317675443789398 2307 44-45 0.729961092752994 2307 46-47 0.7119059772218748 2307 48-49 0.7379734134938047 2307 50-51 0.7038401530708285 2307 52-53 0.716892928405457 2307 54-55 0.7010247897788062 2307 56-57 0.7166646934846597 2307 58-59 0.7134907678273876 2307 60-61 0.6971611595528486 2307 62-63 0.6920189104069081 2307 64-65 0.6743267253697027 2307 66-67 0.7037023561760449 2307 68-69 0.733855886474565 2307 70-71 0.6827173883067843 2307 72-73 0.7898499139355408 2307 74-75 0.8011859252244307 2307 76-77 0.7879904248177212 2307 78-79 0.8499317444293695 2307 80-81 0.8662619445443269 2307 82-83 0.8186210199381065 2307 84-85 0.8282622392010168 2307 86-87 0.8360997053563111 2307 88-89 0.877706425515214 2307 90-91 0.8762090266110931 2307 92-93 0.8661109634635125 2307 94-95 0.8699198302769204 2307 96-97 0.8904056468182304 2307 98-99 0.8514947010621725 2307 100-101 0.8498406988786513 2201 1 0.1312822289191402 2201 2 0.22177992463989682 2201 3 0.2521640040811093 2201 4 0.32644317096016806 2201 5 0.30566205048482686 2201 6 0.3262776897820956 2201 7 0.3192494462532949 2201 8 0.3007035840038981 2201 9 0.3189930380408512 2201 10-11 0.30339233133212673 2201 12-13 0.29218875565506863 2201 14-15 0.5021231133972606 2201 16-17 0.5454388456531873 2201 18-19 0.5036265324590943 2201 20-21 0.5866985826581228 2201 22-23 0.5927238799033958 2201 24-25 0.5656036973583127 2201 26-27 0.5830454381659251 2201 28-29 0.5859175188165366 2201 30-31 0.5853255205209038 2201 32-33 0.6139962484425894 2201 34-35 0.5967977771169615 2201 36-37 0.6210771783958293 2201 38-39 0.5940252133196751 2201 40-41 0.6286897045790454 2201 42-43 0.6074185918712516 2201 44-45 0.5585758707028106 2201 46-47 0.6300191219926461 2201 48-49 0.6612313744989962 2201 50-51 0.5758672910354079 2201 52-53 0.6383744018503421 2201 54-55 0.6294946231957468 2201 56-57 0.6579342006618845 2201 58-59 0.6662613479757766 2201 60-61 0.6835694004122743 2201 62-63 0.6878766372372809 2201 64-65 0.6600148313108178 2201 66-67 0.6851033167439979 2201 68-69 0.701383337607929 2201 70-71 0.7456349054888776 2201 72-73 0.7372524144293635 2201 74-75 0.6998367352354222 2201 76-77 0.7212208610686304 2201 78-79 0.75578866358223 2201 80-81 0.7424321581896649 2201 82-83 0.7231956950631755 2201 84-85 0.7545075875209903 2201 86-87 0.7187203313046666 2201 88-89 0.6829177158480704 2201 90-91 0.7285165199468935 2201 92-93 0.7235008459335148 2201 94-95 0.6913846369776522 2201 96-97 0.7656425434559964 2201 98-99 0.7704976378051782 2201 100-101 0.6590366464385085 1101 1 -0.007051146384092988 1101 2 0.17147304073703395 1101 3 0.2034588594108655 1101 4 0.27240919212978554 1101 5 0.33135484761073286 1101 6 0.32704036679014337 1101 7 0.30549322421573777 1101 8 0.3483045845662929 1101 9 0.3280807803120993 1101 10-11 0.31901268841203034 1101 12-13 0.3423347269644452 1101 14-15 0.5525263601886579 1101 16-17 0.5874076536241049 1101 18-19 0.5977334130043772 1101 20-21 0.5966768002804415 1101 22-23 0.6165690361573368 1101 24-25 0.6168564988490814 1101 26-27 0.6582923890527468 1101 28-29 0.6357409094807522 1101 30-31 0.6494797011425604 1101 32-33 0.6185087260936228 1101 34-35 0.6303598464008786 1101 36-37 0.6280430484738133 1101 38-39 0.729231789163471 1101 40-41 0.7242793396927425 1101 42-43 0.6800652556839566 1101 44-45 0.6493656182958389 1101 46-47 0.6683550994296255 1101 48-49 0.6795657151099093 1101 50-51 0.6317578503262808 1101 52-53 0.6818519437572874 1101 54-55 0.7264057645251896 1101 56-57 0.7119084563257232 1101 58-59 0.728271622207096 1101 60-61 0.7030943011534632 1101 62-63 0.7213022204090365 1101 64-65 0.7389773773727839 1101 66-67 0.7579985262996942 1101 68-69 0.7406950312612395 1101 70-71 0.7511382924597356 1101 72-73 0.7755752552878974 1101 74-75 0.7987729242007049 1101 76-77 0.8091205194336553 1101 78-79 0.8288710588883177 1101 80-81 0.7853571663737995 1101 82-83 0.804173257955064 1101 84-85 0.8260322280488026 1101 86-87 0.8116803423847259 1101 88-89 0.7906267049610847 1101 90-91 0.8597239519766475 1101 92-93 0.8607445567441232 1101 94-95 0.856302334005342 1101 96-97 0.8189946373342636 1101 98-99 0.7733483172978453 1101 100-101 0.7594621792463769 2304 1 -0.06105020910376879 2304 2 0.1255947960547985 2304 3 0.15528199982066582 2304 4 0.20930191258592856 2304 5 0.2614679070499264 2304 6 0.31029624431884884 2304 7 0.23381861670187476 2304 8 0.28560834149826064 2304 9 0.23877553935294316 2304 10-11 0.2874920812626627 2304 12-13 0.29207062870622025 2304 14-15 0.47177803297368115 2304 16-17 0.5106290991186526 2304 18-19 0.5243736535729866 2304 20-21 0.4979640962267027 2304 22-23 0.5281826879056197 2304 24-25 0.5709408280427581 2304 26-27 0.5103876011124271 2304 28-29 0.5457852675739119 2304 30-31 0.4909379205703033 2304 32-33 0.5012365698281656 2304 34-35 0.5259578833193075 2304 36-37 0.5250024199752872 2304 38-39 0.6074984389070224 2304 40-41 0.5908685505547879 2304 42-43 0.6004113985150639 2304 44-45 0.5704915262861547 2304 46-47 0.5979090451194438 2304 48-49 0.6160799435459481 2304 50-51 0.5435194166258484 2304 52-53 0.521607437261423 2304 54-55 0.594145259174887 2304 56-57 0.5700839490487795 2304 58-59 0.5990006505598302 2304 60-61 0.5969105291208692 2304 62-63 0.6194694793847901 2304 64-65 0.6302138063347442 2304 66-67 0.6444099142556468 2304 68-69 0.6556073890419682 2304 70-71 0.6780787556324341 2304 72-73 0.6699833041826864 2304 74-75 0.7051163267886409 2304 76-77 0.7325328381610348 2304 78-79 0.6954877698154363 2304 80-81 0.70940817004481 2304 82-83 0.6647489042879542 2304 84-85 0.7077520072672101 2304 86-87 0.670349371778876 2304 88-89 0.673380421537928 2304 90-91 0.6794867354388501 2304 92-93 0.7030386805171958 2304 94-95 0.7356092672761889 2304 96-97 0.6420629611646191 2304 98-99 0.7174956851022642 2304 100-101 0.7661285855574036 2202 1 0.16406577640697861 2202 2 0.20080549691718375 2202 3 0.20991338895645129 2202 4 0.2709490345446781 2202 5 0.2756083059924066 2202 6 0.3048395332785745 2202 7 0.2574453221457844 2202 8 0.28401303699585867 2202 9 0.28463652815000273 2202 10-11 0.3034062752555968 2202 12-13 0.31032209250182774 2202 14-15 0.4821321165604431 2202 16-17 0.5218241415114022 2202 18-19 0.5024303414558915 2202 20-21 0.5146729697568091 2202 22-23 0.5336719569045982 2202 24-25 0.5901329252492289 2202 26-27 0.5400990761575173 2202 28-29 0.5830301604653911 2202 30-31 0.5729938879070531 2202 32-33 0.5800554431425837 2202 34-35 0.5894805111360739 2202 36-37 0.5697161965679456 2202 38-39 0.6137875737294749 2202 40-41 0.5866274312364297 2202 42-43 0.5911962971845384 2202 44-45 0.6185153738380009 2202 46-47 0.6052784954383625 2202 48-49 0.6567140922684942 2202 50-51 0.609260923603145 2202 52-53 0.6051949128127916 2202 54-55 0.6395572830156269 2202 56-57 0.6196129831707111 2202 58-59 0.6318520707650919 2202 60-61 0.6499353614285823 2202 62-63 0.6493673414712973 2202 64-65 0.6490360666985211 2202 66-67 0.6375411427655493 2202 68-69 0.65832926702533 2202 70-71 0.6595546960884704 2202 72-73 0.694794902222938 2202 74-75 0.697022236629266 2202 76-77 0.6539479082177522 2202 78-79 0.6958215580662497 2202 80-81 0.7093167892411429 2202 82-83 0.6683910252761436 2202 84-85 0.6832292591229248 2202 86-87 0.7114480372218352 2202 88-89 0.7493895408880604 2202 90-91 0.754833336867577 2202 92-93 0.7462128182740457 2202 94-95 0.760982140685968 2202 96-97 0.7414189180578532 2202 98-99 0.7647389291661995 2202 100-101 0.7931425390088123 1102 1 0.05949127760290196 1102 2 0.1283408935594963 1102 3 0.13578385639909385 1102 4 0.19423441254700435 1102 5 0.2189029445000088 1102 6 0.20751282767660229 1102 7 0.2082343572027483 1102 8 0.2161996295234374 1102 9 0.2271785775448123 1102 10-11 0.19993342173636108 1102 12-13 0.21954389305665956 1102 14-15 0.3428569754247093 1102 16-17 0.35778075885588834 1102 18-19 0.390677887534423 1102 20-21 0.31123727832994064 1102 22-23 0.3590450218717294 1102 24-25 0.37402424879028473 1102 26-27 0.38405777284062026 1102 28-29 0.39875415625989064 1102 30-31 0.41203308065507116 1102 32-33 0.4331003802802087 1102 34-35 0.43509457788773176 1102 36-37 0.3704654285524924 1102 38-39 0.46485222893175404 1102 40-41 0.4476253493973772 1102 42-43 0.47537929945602286 1102 44-45 0.4081099955255638 1102 46-47 0.4539281495450709 1102 48-49 0.4774423026392256 1102 50-51 0.3600139091775958 1102 52-53 0.4278594545120953 1102 54-55 0.4805670475348549 1102 56-57 0.45243231550041685 1102 58-59 0.4397398508367232 1102 60-61 0.4927771647855721 1102 62-63 0.5109039587288535 1102 64-65 0.527653030464748 1102 66-67 0.5370773849913562 1102 68-69 0.5100598771260323 1102 70-71 0.514751497054867 1102 72-73 0.5409753721406787 1102 74-75 0.5230924280305516 1102 76-77 0.535157626349708 1102 78-79 0.5639807296105701 1102 80-81 0.5141972293264985 1102 82-83 0.5532747116483065 1102 84-85 0.517467546609268 1102 86-87 0.5256868666649623 1102 88-89 0.5227964274665169 1102 90-91 0.5345021780236578 1102 92-93 0.4943580615539105 1102 94-95 0.5752783249157645 1102 96-97 0.632288151812503 1102 98-99 0.5506378485434595 1102 100-101 0.5013895611706047 2305 1 0.12586444445945233 2305 2 0.1265480056333601 2305 3 0.16003306815529328 2305 4 0.1718396784761964 2305 5 0.19850521895391182 2305 6 0.2061815523681787 2305 7 0.2037599018062508 2305 8 0.2331462739661916 2305 9 0.24055774627322535 2305 10-11 0.2311431602383749 2305 12-13 0.19156417668109071 2305 14-15 0.32228081158849164 2305 16-17 0.3571133533032409 2305 18-19 0.38744184499707046 2305 20-21 0.369015018519967 2305 22-23 0.390471351823507 2305 24-25 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-1.0622390152577736 1213 90-91 -1.0853134715366295 1213 92-93 -1.0615413880633895 1213 94-95 -1.1789343148214542 1213 96-97 -1.278040243302307 1213 98-99 -1.1115797380140577 1213 100-101 -1.1033323813431508 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15886.0 3 1734.0 4 1053.0 5 771.0 6 806.0 7 1596.0 8 2292.0 9 3649.0 10 7025.0 11 12373.0 12 16025.0 13 19230.0 14 21461.0 15 23804.0 16 26968.0 17 30461.0 18 33864.0 19 37934.0 20 42295.0 21 47059.0 22 53277.0 23 59543.0 24 67441.0 25 77735.0 26 89758.0 27 108694.0 28 129738.0 29 155960.0 30 191988.0 31 244456.0 32 321812.0 33 434656.0 34 609512.0 35 979241.0 36 2264776.0 37 6288745.0 38 5338716.0 39 3147.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.04503318710165 12.38511018789732 11.311565036041907 55.25829158895912 2 24.97356231413292 19.34039044797954 36.01498842040633 19.671058817481207 3 25.301821071080393 24.6281754613027 22.816614817779296 27.25338864983761 4 27.567113603066833 28.67681897725677 16.884847146262917 26.87122027341348 5 29.76450891258995 30.154926952425633 20.150065119471765 19.930499015512652 6 21.390546267922005 33.603803166480496 21.25313632310687 23.75251424249063 7 20.12409697986316 14.424119936784505 40.22024444331495 25.23153864003739 8 21.86779601211066 19.649071282680968 26.15990561840831 32.32322708680006 9 22.51981417227152 19.100528528171193 30.163781306707698 28.215875992849586 10-11 25.890900159383435 27.591268035558684 20.22175662035576 26.296075184702122 12-13 23.825419953393677 21.961330414041335 26.776460778645315 27.43678885391967 14-15 24.628740463764593 23.625224345746016 25.205128490560536 26.540906699928858 16-17 25.957419103723694 23.821886212596144 23.876936223919277 26.343758459760885 18-19 25.329129738600965 24.26729963508106 24.024954074084594 26.37861655223338 20-21 25.43785823608342 24.337897783759935 24.33829808100601 25.885945899150638 22-23 25.447465270081754 24.29553694254059 24.21280599430576 26.044191793071896 24-25 25.12578986463779 24.52669844219207 24.30646753583202 26.041044157338117 26-27 25.23507402360636 24.537009182361306 24.4454045344498 25.782512259582536 28-29 25.503395630138282 24.10612212139637 24.158441923835714 26.232040324629637 30-31 25.21627222380384 24.17093417374672 24.358471045452987 26.254322556996453 32-33 25.40118417186912 24.41612949866812 24.345464376517924 25.837221952944837 34-35 25.688921860841024 24.245887055641212 23.947736224796202 26.117454858721562 36-37 25.366452313280156 24.420528126421477 23.987776666318556 26.22524289397981 38-39 25.498754438310346 24.49699619055713 24.27855935272497 25.725690018407555 40-41 25.72390665212935 24.163958537928167 24.097191526244107 26.01494328369838 42-43 25.14475891232295 24.1936716647816 24.473655196919356 26.18791422597609 44-45 25.603766648336425 24.27699188676722 24.330959682159527 25.78828178273683 46-47 25.747112043839028 24.075869391869187 24.071404145062676 26.105614419229113 48-49 25.435850098367514 24.098060964228875 24.224914931148945 26.24117400625467 50-51 25.600771408498456 24.280996939550047 24.29147760431623 25.82675404763527 52-53 25.908420204447 23.98043948580381 24.03978156187995 26.071358747869233 54-55 25.358038039535085 24.08758161599882 24.308172364288946 26.24620798017715 56-57 25.55785387506051 24.315256741899226 24.365145236633506 25.761744146406755 58-59 25.74184291045737 24.219043249898554 23.986076126768513 26.053037712875565 60-61 25.338161361345428 24.24908805971217 24.25570687890018 26.157043700042216 62-63 25.567252754292742 24.269864470904746 24.291889628660854 25.87099314614165 64-65 25.69906149360734 24.192370352977736 23.967693827917174 26.140874325497755 66-67 25.4037456945671 24.239891939379664 24.228692691887932 26.127669674165304 68-69 25.692232635751022 24.374694166859516 24.18021782477549 25.752855372613965 70-71 25.798523354623832 24.205817399506472 23.988193599606642 26.007465646263057 72-73 25.4525298998203 24.21677192181939 24.232874122833024 26.097824055527287 74-75 25.684156834227593 24.446273930654066 24.210823114875428 25.658746120242913 76-77 25.858805640388677 24.27584422428188 23.918715144039346 25.946634991290097 78-79 25.525814600438924 24.274984402469318 24.21813104722347 25.981069949868292 80-81 25.866478835640034 24.475169282412125 24.032051487286232 25.626300394661605 82-83 25.9127690791926 24.294560215386216 23.860441257244663 25.932229448176525 84-85 25.48570943535419 24.327271299774804 24.222158827400328 25.964860437470676 86-87 25.78978665065039 24.567369873375903 24.0377119191173 25.605131556856403 88-89 25.884606151315065 24.335126345093457 23.856136020345765 25.92413148324571 90-91 25.669152792304367 24.23422341760682 24.030465960427737 26.06615782966108 92-93 25.79596529172199 24.51431604332032 24.064543769483624 25.625174895474068 94-95 25.960831395969702 24.293339629823837 23.90210441223003 25.843724561976426 96-97 25.636881283173302 24.407515321005636 24.062900293930795 25.89270310189027 98-99 25.877269457147168 24.510052656962415 24.005664351341267 25.60701353454915 100-101 26.129974404446155 24.299293432204493 23.65461050891573 25.91612165443362 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13864.0 1 7383.5 2 847.0 3 738.5 4 672.0 5 598.0 6 514.5 7 446.5 8 382.5 9 373.5 10 376.0 11 382.5 12 418.0 13 447.0 14 480.5 15 537.5 16 613.5 17 696.0 18 818.0 19 962.5 20 1185.5 21 1551.0 22 2024.5 23 2767.5 24 3886.5 25 5595.0 26 8176.0 27 11774.5 28 16825.0 29 23926.0 30 33045.5 31 45210.5 32 61191.5 33 82010.5 34 109691.0 35 144990.0 36 188482.5 37 245018.0 38 311750.0 39 382104.0 40 453412.0 41 522111.5 42 588692.0 43 638052.5 44 665066.5 45 676222.5 46 673439.0 47 659618.0 48 636273.0 49 611401.5 50 586071.0 51 558845.0 52 541644.5 53 537576.5 54 538296.5 55 542392.0 56 550521.5 57 551256.5 58 536826.0 59 505436.0 60 481763.5 61 466874.5 62 443984.5 63 413361.0 64 366262.0 65 332498.5 66 319653.5 67 296069.5 68 259312.5 69 223162.5 70 190662.5 71 160046.5 72 132834.0 73 108611.0 74 84924.5 75 63232.0 76 45323.0 77 32360.0 78 22459.0 79 14955.5 80 9720.0 81 6172.5 82 3778.0 83 2371.0 84 1497.0 85 1049.0 86 806.0 87 600.5 88 478.5 89 392.0 90 326.0 91 274.0 92 205.0 93 158.5 94 116.5 95 78.5 96 58.5 97 37.5 98 17.0 99 10.0 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15177185464328266 2 0.14439800419701554 3 0.15239666181850073 4 0.1478597736813318 5 0.15375322514487505 6 0.1046129851479957 7 0.10806912573884152 8 0.10582319724413879 9 0.09326513591160296 10-11 0.08028772201551988 12-13 0.08861848435176059 14-15 0.13291787596406762 16-17 0.14212111678822545 18-19 0.1297319222598026 20-21 0.161332530202813 22-23 0.14322719435516548 24-25 0.1395008668777389 26-27 0.1290789706172324 28-29 0.1276464172290072 30-31 0.09252775086697625 32-33 0.10354349538861346 34-35 0.1188062400336923 36-37 0.12406081208834142 38-39 0.12202878154551515 40-41 0.15580495681484785 42-43 0.13655132669923206 44-45 0.12660507193697712 46-47 0.16042064946060283 48-49 0.18289400664130626 50-51 0.15792704965320106 52-53 0.1081845180549854 54-55 0.10974372154629532 56-57 0.11205156786917281 58-59 0.11169976202727075 60-61 0.11609029893420843 62-63 0.0990375661655319 64-65 0.11616066010258883 66-67 0.1306888341497762 68-69 0.12925628076155102 70-71 0.11746656338772928 72-73 0.1270159811603187 74-75 0.16249489670445738 76-77 0.19102775770608182 78-79 0.1660551718245062 80-81 0.13910684433480863 82-83 0.12229896843209594 84-85 0.12978821119450693 86-87 0.12304761126366351 88-89 0.12625326609507506 90-91 0.1408686879910541 92-93 0.10788900114778767 94-95 0.11339968785534149 96-97 0.11436504308552074 98-99 0.11718793316094285 100-101 0.11046984880398115 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.7765481E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.13946075317912 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29443149030537 37.25082731887921 2 13.170355701372971 13.20709065588798 3 4.629589616360232 6.9637538061845845 4 2.4181349331988367 4.849759263160579 5 1.4117232811070652 3.5391522023708473 6 0.9298434195696531 2.79731085852687 7 0.5573278656299955 1.9560883051785771 8 0.3889836426567125 1.560274406769191 9 0.34035255808218684 1.535858435538533 >10 1.7013593468952717 15.493049842829848 >50 0.10177174945924057 3.5561874449126227 >100 0.05085271366714075 4.568624315107557 >500 0.003792657054779447 1.303677941510948 >1k 0.001445528180727692 1.2995329008748302 >5k 3.5496459808107646E-5 0.11881230226779661 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.025853507709698374 3.3773360822597485E-5 0.0 2 0.026804790706201537 3.94022542930304E-5 5.628893470432914E-6 3 0.030548004864039427 3.94022542930304E-5 5.628893470432914E-6 4 0.04485102317240946 4.503114776346331E-5 1.1257786940865828E-5 5 0.047406540807986 5.066004123389623E-5 1.1257786940865828E-5 6 0.054510204367672344 7.317561511562788E-5 2.2515573881731657E-5 7 0.056181985728390914 7.88045085860608E-5 2.2515573881731657E-5 8 0.06024604681404348 8.443340205649371E-5 2.2515573881731657E-5 9 0.06581865134977206 9.006229552692663E-5 2.814446735216457E-5 10-11 0.07635312547968726 1.0694897593822537E-4 2.814446735216457E-5 12-13 0.08799086272980731 1.238356563495241E-4 2.814446735216457E-5 14-15 0.1008472554162761 1.3509344329038994E-4 3.3773360822597485E-5 16-17 0.1139822783295313 1.5198012370168867E-4 3.3773360822597485E-5 18-19 0.12732557030119251 1.6605235737777096E-4 3.3773360822597485E-5 20-21 0.13748853746205913 1.7449569758342032E-4 3.3773360822597485E-5 22-23 0.14786258812806702 2.1389795187645074E-4 3.3773360822597485E-5 24-25 0.156989838890374 2.307846322877495E-4 3.658780755781394E-5 26-27 0.1651517344225017 2.7581578005121275E-4 4.2216701028246856E-5 28-29 0.17287176181720043 3.067746941385938E-4 4.503114776346331E-5 30-31 0.17982625969992033 3.405480549611913E-4 4.784559449867977E-5 32-33 0.1857562989710214 3.5743473537249E-4 6.47322749099785E-5 34-35 0.19168070934865203 3.6587807557813943E-4 8.443340205649371E-5 36-37 0.1970450448259746 3.855792027246546E-4 9.850563573257599E-5 38-39 0.20261202046823273 3.94022542930304E-4 1.0132008246779245E-4 40-41 0.20791443811738056 4.165381168120357E-4 1.1257786940865828E-4 42-43 0.21275247205521763 4.277959037529015E-4 1.238356563495241E-4 44-45 0.2173737935944431 4.446825841642002E-4 1.2946454981995704E-4 46-47 0.22238632322986357 4.5594037110506606E-4 1.2946454981995704E-4 48-49 0.22717088267973157 4.7001260478114835E-4 1.3509344329038994E-4 50-51 0.23195262768286432 4.756414982515812E-4 1.3509344329038994E-4 52-53 0.23636849461041892 4.784559449867977E-4 1.4072233676082287E-4 54-55 0.24112490959293476 4.8408483845723064E-4 1.519801237016887E-4 56-57 0.24549293092599067 4.953426253980964E-4 1.6323791064255452E-4 58-59 0.24963016762675888 5.066004123389623E-4 1.7731014431863682E-4 60-61 0.2542768191866013 5.347448796911268E-4 1.8012459105385325E-4 62-63 0.2590895231038214 5.628893470432915E-4 1.8012459105385325E-4 64-65 0.2637446180038694 5.741471339841572E-4 1.8856793125950262E-4 66-67 0.2683462384159483 5.769615807193737E-4 2.026401649355849E-4 68-69 0.2731195400788754 5.882193676602396E-4 2.0545461167080137E-4 70-71 0.27815458528817766 6.135493882771877E-4 2.0826905840601784E-4 72-73 0.2838172521194332 6.191782817476205E-4 2.0826905840601784E-4 74-75 0.28981483811217945 6.332505154237029E-4 2.0826905840601784E-4 76-77 0.29594751754821613 6.473227490997851E-4 2.0826905840601784E-4 78-79 0.302966747705846 6.613949827758675E-4 2.1108350514123427E-4 80-81 0.31116804549226673 6.782816631871662E-4 2.2234129208210013E-4 82-83 0.32128879595210513 6.923538968632485E-4 2.476713126990482E-4 84-85 0.3333346279788315 7.120550240097636E-4 2.589290996399141E-4 86-87 0.34820335008098 7.289417044210624E-4 2.6455799311034695E-4 88-89 0.3658611888977281 7.683439587140928E-4 2.6455799311034695E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGAAT 7965 0.0 11.86833 1 CGAATTA 6015 0.0 10.820644 14-15 CGCGTAT 1760 0.0 10.796167 1 CTCGTAT 4365 0.0 10.66508 1 CCGAGAT 14650 0.0 10.570663 1 CGGAAAT 5300 0.0 10.307283 1 CTCGGAT 8855 0.0 10.299946 1 CCGGAAT 6330 0.0 10.206045 1 CGATTTA 3635 0.0 10.192814 4 CGCAAAT 3990 0.0 10.119716 1 CGGGAAT 5775 0.0 10.117552 1 CGAGAAT 6250 0.0 9.804647 1 CTGGAAT 15080 0.0 9.733732 1 CTCGGGT 8830 0.0 9.732808 8 GTGCACA 8740 0.0 9.724462 6 CCGGGAT 8310 0.0 9.71785 1 CGGAGAT 17195 0.0 9.696774 1 TGCACAC 9360 0.0 9.68862 7 CGGGGAT 8115 0.0 9.424528 1 GTTTGGT 12675 0.0 9.406933 1 >>END_MODULE