FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 mx24-1.3420000000ca5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 mx24-1.3420000000ca5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17765481
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAT79650.011.868331
CGAATTA60150.010.82064414-15
CGCGTAT17600.010.7961671
CTCGTAT43650.010.665081
CCGAGAT146500.010.5706631
CGGAAAT53000.010.3072831
CTCGGAT88550.010.2999461
CCGGAAT63300.010.2060451
CGATTTA36350.010.1928144
CGCAAAT39900.010.1197161
CGGGAAT57750.010.1175521
CGAGAAT62500.09.8046471
CTGGAAT150800.09.7337321
CTCGGGT88300.09.7328088
GTGCACA87400.09.7244626
CCGGGAT83100.09.717851
CGGAGAT171950.09.6967741
TGCACAC93600.09.688627
CGGGGAT81150.09.4245281
GTTTGGT126750.09.4069331
Produced by FastQC (version 0.11.2)