##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 mx12-2.3420000000cd81.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 21362566 Sequences flagged as poor quality 0 Sequence length 101 %GC 51 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.52657545914662 33.0 33.0 33.0 33.0 33.0 2 32.517198823399774 33.0 33.0 33.0 33.0 33.0 3 32.51108850874937 33.0 33.0 33.0 33.0 33.0 4 36.39429434647504 37.0 37.0 37.0 37.0 37.0 5 36.38086688649669 37.0 37.0 37.0 37.0 37.0 6 36.38005172225097 37.0 37.0 37.0 37.0 37.0 7 36.42120932475996 37.0 37.0 37.0 37.0 37.0 8 36.42258603203379 37.0 37.0 37.0 37.0 37.0 9 36.421972341712134 37.0 37.0 37.0 37.0 37.0 10-11 36.40289593487974 37.0 37.0 37.0 37.0 37.0 12-13 36.389116106183124 37.0 37.0 37.0 37.0 37.0 14-15 38.731334124374385 40.0 40.0 40.0 37.0 40.0 16-17 38.713557608201185 40.0 40.0 40.0 37.0 40.0 18-19 38.68557801061914 40.0 40.0 40.0 37.0 40.0 20-21 38.64857964628406 40.0 40.0 40.0 37.0 40.0 22-23 38.621735282175365 40.0 40.0 40.0 37.0 40.0 24-25 38.58409123229859 40.0 40.0 40.0 37.0 40.0 26-27 38.541647454711196 40.0 40.0 40.0 37.0 40.0 28-29 38.49627050888924 40.0 40.0 40.0 37.0 40.0 30-31 38.444218545655986 40.0 37.0 40.0 37.0 40.0 32-33 38.37429267626371 40.0 37.0 40.0 37.0 40.0 34-35 38.29888548969258 40.0 37.0 40.0 37.0 40.0 36-37 38.21600684580682 40.0 37.0 40.0 37.0 40.0 38-39 38.10443520689415 40.0 37.0 40.0 37.0 40.0 40-41 37.99421146317348 40.0 37.0 40.0 37.0 40.0 42-43 37.88948029464251 40.0 37.0 40.0 33.0 40.0 44-45 37.775803946024084 40.0 37.0 40.0 33.0 40.0 46-47 37.631750815889816 40.0 37.0 40.0 33.0 40.0 48-49 37.48558291171575 40.0 37.0 40.0 33.0 40.0 50-51 36.03526563709622 38.5 35.0 38.5 30.0 38.5 52-53 36.38148202795489 37.0 37.0 38.5 33.0 40.0 54-55 37.06407146969142 37.0 37.0 40.0 33.0 40.0 56-57 37.01474062151522 37.0 37.0 40.0 33.0 40.0 58-59 36.868705332496106 37.0 37.0 40.0 33.0 40.0 60-61 36.69213792013562 37.0 37.0 40.0 33.0 40.0 62-63 36.51080083263406 37.0 37.0 40.0 33.0 40.0 64-65 36.32398785801294 37.0 37.0 40.0 33.0 40.0 66-67 36.09685865920789 37.0 37.0 38.5 33.0 40.0 68-69 35.88452059083164 37.0 37.0 37.0 33.0 40.0 70-71 35.68901128263337 37.0 37.0 37.0 33.0 40.0 72-73 35.47962438126581 37.0 37.0 37.0 33.0 40.0 74-75 35.29268295765593 37.0 37.0 37.0 33.0 37.0 76-77 35.113384599958636 37.0 37.0 37.0 33.0 37.0 78-79 34.968050022642416 37.0 35.0 37.0 33.0 37.0 80-81 34.85079409467945 37.0 33.0 37.0 33.0 37.0 82-83 34.76187544604895 37.0 33.0 37.0 33.0 37.0 84-85 34.66773584222045 37.0 33.0 37.0 33.0 37.0 86-87 34.5810593399688 37.0 33.0 37.0 33.0 37.0 88-89 34.503212207746955 37.0 33.0 37.0 30.0 37.0 90-91 34.408180974139526 37.0 33.0 37.0 27.0 37.0 92-93 34.34927077580474 37.0 33.0 37.0 27.0 37.0 94-95 34.27882472077559 37.0 33.0 37.0 27.0 37.0 96-97 34.21519451361789 37.0 33.0 37.0 27.0 37.0 98-99 34.1437846464699 37.0 33.0 37.0 27.0 37.0 100-101 32.38972190887555 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.0903879501182061 2306 2 0.2032976794607606 2306 3 0.22135814182225033 2306 4 0.28973549261935716 2306 5 0.28474757298802444 2306 6 0.33584626787727245 2306 7 0.307454748809036 2306 8 0.29137240271325027 2306 9 0.31602267535964046 2306 10-11 0.3310445675898208 2306 12-13 0.3369143226487026 2306 14-15 0.5783637373351311 2306 16-17 0.5174952503482118 2306 18-19 0.5944952355150832 2306 20-21 0.6331173359386 2306 22-23 0.6156844509264872 2306 24-25 0.5964933385660558 2306 26-27 0.6639103957943746 2306 28-29 0.6871006021777148 2306 30-31 0.6904904547541051 2306 32-33 0.6357043211249618 2306 34-35 0.6713607027762336 2306 36-37 0.6736953013765898 2306 38-39 0.7051561863095728 2306 40-41 0.7409325135519254 2306 42-43 0.7423564216525236 2306 44-45 0.7225836281143856 2306 46-47 0.703489105843552 2306 48-49 0.7532988126171887 2306 50-51 0.6502543412882247 2306 52-53 0.6713297218741019 2306 54-55 0.7436952089623645 2306 56-57 0.6809158782197642 2306 58-59 0.7452588807927185 2306 60-61 0.7368505714888158 2306 62-63 0.7186486244956498 2306 64-65 0.76440907829803 2306 66-67 0.7398316220434324 2306 68-69 0.7237688299359206 2306 70-71 0.7227891517199225 2306 72-73 0.7463943463994056 2306 74-75 0.7565820061823771 2306 76-77 0.7002498476992116 2306 78-79 0.6819531109052974 2306 80-81 0.7432821138013139 2306 82-83 0.7460229379444812 2306 84-85 0.7583477550965796 2306 86-87 0.7721296863827405 2306 88-89 0.8059530627104721 2306 90-91 0.8510245647984505 2306 92-93 0.832011246389925 2306 94-95 0.7842639650007612 2306 96-97 0.7527781177202044 2306 98-99 0.8179471318864842 2306 100-101 0.7177179245445231 2307 1 -0.06208353997482163 2307 2 0.10943657230994575 2307 3 0.21893850061973552 2307 4 0.2696058394258358 2307 5 0.2883681666216944 2307 6 0.31051831256337437 2307 7 0.3029218782276075 2307 8 0.3146112102385601 2307 9 0.3126888991744039 2307 10-11 0.3279329874901791 2307 12-13 0.3192992920688482 2307 14-15 0.5696649671842522 2307 16-17 0.5629316614698965 2307 18-19 0.5989929582685747 2307 20-21 0.6139016750355637 2307 22-23 0.630851556882817 2307 24-25 0.6363065344031824 2307 26-27 0.6474160669299565 2307 28-29 0.6600447107860461 2307 30-31 0.6694660939727797 2307 32-33 0.6512927486517626 2307 34-35 0.6732107166082741 2307 36-37 0.6765319317706897 2307 38-39 0.6744407614862737 2307 40-41 0.6867283056735118 2307 42-43 0.6822331276747207 2307 44-45 0.7027047276678999 2307 46-47 0.6581715816350595 2307 48-49 0.6942545056484448 2307 50-51 0.6450194765616644 2307 52-53 0.6593236221760748 2307 54-55 0.6607370114700046 2307 56-57 0.6818614297804828 2307 58-59 0.6721869472729622 2307 60-61 0.6772880974314717 2307 62-63 0.6673621812044601 2307 64-65 0.6862106957842471 2307 66-67 0.7264587524383828 2307 68-69 0.7542858488074842 2307 70-71 0.715197193562382 2307 72-73 0.7292936120019817 2307 74-75 0.7569523684117527 2307 76-77 0.7505751374008653 2307 78-79 0.7897925034565958 2307 80-81 0.8087046722401539 2307 82-83 0.8024600696922661 2307 84-85 0.83109451731886 2307 86-87 0.8143205111875318 2307 88-89 0.8393832253699003 2307 90-91 0.8170392807996336 2307 92-93 0.8449111701983512 2307 94-95 0.8507056191813476 2307 96-97 0.8456365569514119 2307 98-99 0.8055797506495281 2307 100-101 0.7431152738208482 2201 1 0.11435569694387482 2201 2 0.19984843014286469 2201 3 0.22340404935067681 2201 4 0.27172212862470246 2201 5 0.28046965782252187 2201 6 0.31456277918323394 2201 7 0.2986132964030688 2201 8 0.2921361256285806 2201 9 0.2970796687697046 2201 10-11 0.2980210492212265 2201 12-13 0.308709282629529 2201 14-15 0.5075045076500686 2201 16-17 0.5260192806506367 2201 18-19 0.48446777073496605 2201 20-21 0.5516842890057134 2201 22-23 0.5831967210747635 2201 24-25 0.5677382419819921 2201 26-27 0.5630546747435545 2201 28-29 0.5860202106609833 2201 30-31 0.5841867826472082 2201 32-33 0.5850970655409142 2201 34-35 0.6106259494071864 2201 36-37 0.6157973530211862 2201 38-39 0.6381273400093903 2201 40-41 0.6424946764343176 2201 42-43 0.6123036862010096 2201 44-45 0.5618601660868663 2201 46-47 0.6243835598887273 2201 48-49 0.6434593503521242 2201 50-51 0.5497689553503946 2201 52-53 0.6330544800745201 2201 54-55 0.6155053770037853 2201 56-57 0.605746690877055 2201 58-59 0.6380411956646981 2201 60-61 0.6603184964692446 2201 62-63 0.6505411510674222 2201 64-65 0.6434117973217042 2201 66-67 0.6441929521343681 2201 68-69 0.6819355845857729 2201 70-71 0.6668920126705729 2201 72-73 0.6761526154512936 2201 74-75 0.6559121241256065 2201 76-77 0.7038701643849734 2201 78-79 0.6691982779723844 2201 80-81 0.6647678245657147 2201 82-83 0.6644429207914797 2201 84-85 0.6900991305736994 2201 86-87 0.6872731099204756 2201 88-89 0.6661407399513308 2201 90-91 0.6802456166635693 2201 92-93 0.6635579714308761 2201 94-95 0.7154476213751337 2201 96-97 0.7274613968958832 2201 98-99 0.7075590678379058 2201 100-101 0.6273851966489161 1101 1 -0.01721438883356541 1101 2 0.163508939051205 1101 3 0.196813895185997 1101 4 0.25335417825216666 1101 5 0.2941469832657404 1101 6 0.3172453688902692 1101 7 0.2880908823878485 1101 8 0.31799756328168627 1101 9 0.3153266335162783 1101 10-11 0.2840786830593771 1101 12-13 0.31841082929180686 1101 14-15 0.510372493098032 1101 16-17 0.5455063132337372 1101 18-19 0.5669367420973614 1101 20-21 0.5735893090200079 1101 22-23 0.5784330401024604 1101 24-25 0.5775777588065623 1101 26-27 0.6099235006904493 1101 28-29 0.6188088109188357 1101 30-31 0.6112117450394692 1101 32-33 0.5942315499600497 1101 34-35 0.6371024239599663 1101 36-37 0.5770677094694392 1101 38-39 0.6809295639551536 1101 40-41 0.6876080306970778 1101 42-43 0.6425759731087908 1101 44-45 0.6337791138307196 1101 46-47 0.6580669153545387 1101 48-49 0.6636621633355446 1101 50-51 0.5972465898507053 1101 52-53 0.6557452920181248 1101 54-55 0.7021128754737447 1101 56-57 0.7016804822451519 1101 58-59 0.6825535513924379 1101 60-61 0.6736383844966838 1101 62-63 0.6749965231635784 1101 64-65 0.662593001713411 1101 66-67 0.6965637762330843 1101 68-69 0.6929085334028571 1101 70-71 0.6646576139403706 1101 72-73 0.6830114260535183 1101 74-75 0.7164158490044699 1101 76-77 0.7671478676593466 1101 78-79 0.7791694095875101 1101 80-81 0.7228899814485743 1101 82-83 0.749878150251952 1101 84-85 0.7210915905049546 1101 86-87 0.728978526188719 1101 88-89 0.6595379676488591 1101 90-91 0.7360116516229454 1101 92-93 0.7761665946090446 1101 94-95 0.7697901800912277 1101 96-97 0.7755783301630927 1101 98-99 0.7599992396550306 1101 100-101 0.7451522509263313 2304 1 -0.033100874407928416 2304 2 0.13115649930988837 2304 3 0.18418696706426374 2304 4 0.21630640032278592 2304 5 0.2295235190211713 2304 6 0.28227538560142307 2304 7 0.2506254030240882 2304 8 0.2772559894910174 2304 9 0.22767398440846165 2304 10-11 0.2831059633932753 2304 12-13 0.2901135528179992 2304 14-15 0.48238650493030377 2304 16-17 0.5226904942212443 2304 18-19 0.5061282165729466 2304 20-21 0.5094587918233628 2304 22-23 0.5146332703266623 2304 24-25 0.5442127071227674 2304 26-27 0.4802906949201855 2304 28-29 0.5331693797256847 2304 30-31 0.5036584026840671 2304 32-33 0.5119213096569482 2304 34-35 0.5462149276384238 2304 36-37 0.5485807418390962 2304 38-39 0.5838592666978784 2304 40-41 0.597647239752007 2304 42-43 0.5925797522517939 2304 44-45 0.6102442697157002 2304 46-47 0.6186572107454538 2304 48-49 0.672607569379565 2304 50-51 0.5979029149041466 2304 52-53 0.5630996372013541 2304 54-55 0.6043701446900087 2304 56-57 0.6090559906291588 2304 58-59 0.6362908659203299 2304 60-61 0.6436660946411408 2304 62-63 0.649103009771899 2304 64-65 0.6501941810758254 2304 66-67 0.6550361030071841 2304 68-69 0.6645233249010971 2304 70-71 0.705678187674188 2304 72-73 0.6890334279264678 2304 74-75 0.7026578422304297 2304 76-77 0.7257370106199375 2304 78-79 0.708173657582293 2304 80-81 0.722469665363036 2304 82-83 0.7219406124828467 2304 84-85 0.7364168544984224 2304 86-87 0.7216976650027718 2304 88-89 0.7031579847198728 2304 90-91 0.7159980205219796 2304 92-93 0.7104108257224695 2304 94-95 0.7220782994139157 2304 96-97 0.6852359449656191 2304 98-99 0.7441421679143616 2304 100-101 0.8061545939156431 2202 1 0.16143696403710805 2202 2 0.18287884858209225 2202 3 0.19117383428845613 2202 4 0.24354470169975428 2202 5 0.237627522497192 2202 6 0.25477450891727216 2202 7 0.21503651274959878 2202 8 0.24642274243074525 2202 9 0.258542491571518 2202 10-11 0.25317242854862343 2202 12-13 0.2683189701282913 2202 14-15 0.4220218525598085 2202 16-17 0.4641074009325621 2202 18-19 0.46612881685975793 2202 20-21 0.48726166436739504 2202 22-23 0.4942732807327843 2202 24-25 0.5086085069047144 2202 26-27 0.45626586401530744 2202 28-29 0.5091295035875376 2202 30-31 0.5331454543144432 2202 32-33 0.5099495830482255 2202 34-35 0.5404692610159643 2202 36-37 0.4953394468551764 2202 38-39 0.5922830384750881 2202 40-41 0.566764073175257 2202 42-43 0.5690116123436368 2202 44-45 0.5705104090268733 2202 46-47 0.5941975784817473 2202 48-49 0.6170606252707671 2202 50-51 0.561769036314665 2202 52-53 0.5712860174818033 2202 54-55 0.6192691938565247 2202 56-57 0.5887996982639834 2202 58-59 0.6128592057937539 2202 60-61 0.6150421456201514 2202 62-63 0.599337663679826 2202 64-65 0.6008088257411686 2202 66-67 0.5852328557302755 2202 68-69 0.6089685502120616 2202 70-71 0.6511723439488364 2202 72-73 0.6867578744585146 2202 74-75 0.6564505190614014 2202 76-77 0.6701309461493921 2202 78-79 0.7243901387238907 2202 80-81 0.7081575515768748 2202 82-83 0.6776275492344936 2202 84-85 0.680965510597666 2202 86-87 0.7049434205800154 2202 88-89 0.7353578410448165 2202 90-91 0.7199034661860253 2202 92-93 0.7362828223381044 2202 94-95 0.7481480017442763 2202 96-97 0.7357210504493992 2202 98-99 0.7471416244416389 2202 100-101 0.8293588817434525 1102 1 0.06631407575441983 1102 2 0.1363396599222142 1102 3 0.13507152361380292 1102 4 0.19687577158742897 1102 5 0.21841011597725668 1102 6 0.22686905052903938 1102 7 0.20118676611958364 1102 8 0.20040850060205884 1102 9 0.23153085241224147 1102 10-11 0.21050097776092258 1102 12-13 0.24756844829561686 1102 14-15 0.3564333762899565 1102 16-17 0.38500193989912646 1102 18-19 0.41299545132966387 1102 20-21 0.36306730245738805 1102 22-23 0.4070067846635368 1102 24-25 0.38335545382684444 1102 26-27 0.40560937409780706 1102 28-29 0.42537667899750176 1102 30-31 0.4403547659569469 1102 32-33 0.4508282991447672 1102 34-35 0.4488642666487479 1102 36-37 0.42886049450005004 1102 38-39 0.4478404002441607 1102 40-41 0.4857706427365116 1102 42-43 0.4968607144888182 1102 44-45 0.4535479263530604 1102 46-47 0.4943450257726454 1102 48-49 0.5083123427764775 1102 50-51 0.4048709392906673 1102 52-53 0.462964997908351 1102 54-55 0.5398434779777261 1102 56-57 0.4908569919428345 1102 58-59 0.5023381137446066 1102 60-61 0.5227817393847829 1102 62-63 0.5692392129530575 1102 64-65 0.5497940332555018 1102 66-67 0.5603427663871869 1102 68-69 0.555924831308559 1102 70-71 0.5446113887674429 1102 72-73 0.5489256296344394 1102 74-75 0.5725777992639962 1102 76-77 0.5732501688078528 1102 78-79 0.6477609442097503 1102 80-81 0.5803505977720818 1102 82-83 0.6316792131326636 1102 84-85 0.5848863549940617 1102 86-87 0.6139600814891395 1102 88-89 0.6738580707050374 1102 90-91 0.6480511660170833 1102 92-93 0.5908962772585511 1102 94-95 0.6434480418363577 1102 96-97 0.7071040703350846 1102 98-99 0.6569682008328002 1102 100-101 0.6360771597705579 2305 1 0.11273250722805273 2305 2 0.0960689973735569 2305 3 0.12687261249317316 2305 4 0.14752158890670586 2305 5 0.15026146790914652 2305 6 0.17708469375342162 2305 7 0.1806228909241554 2305 8 0.1877073371566098 2305 9 0.2174703158127258 2305 10-11 0.20287627138399245 2305 12-13 0.20190296532690866 2305 14-15 0.3297358763244773 2305 16-17 0.34747548313747956 2305 18-19 0.3753183952777661 2305 20-21 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-1.101359118096184 1213 58-59 -1.0023688467303131 1213 60-61 -1.0008938061221428 1213 62-63 -1.013251278043164 1213 64-65 -1.0702586006206047 1213 66-67 -1.0460244796196037 1213 68-69 -1.1799440399357835 1213 70-71 -1.2058170057788828 1213 72-73 -0.9403293014515341 1213 74-75 -0.9718089437039836 1213 76-77 -0.950055471086543 1213 78-79 -0.9169673050176073 1213 80-81 -0.9956562750603979 1213 82-83 -0.9831270201671956 1213 84-85 -1.0693122556223216 1213 86-87 -1.047829112801864 1213 88-89 -1.037591558448284 1213 90-91 -1.026048761203164 1213 92-93 -1.0421191254940325 1213 94-95 -1.1347762750056205 1213 96-97 -1.2111307051329803 1213 98-99 -1.0820436218549787 1213 100-101 -1.0449547982599086 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19594.0 3 2004.0 4 1308.0 5 993.0 6 948.0 7 1553.0 8 2353.0 9 3503.0 10 7407.0 11 12573.0 12 15867.0 13 18473.0 14 20700.0 15 23477.0 16 26919.0 17 30307.0 18 34509.0 19 39128.0 20 44064.0 21 49533.0 22 55827.0 23 63130.0 24 72309.0 25 83949.0 26 99436.0 27 121860.0 28 142904.0 29 169430.0 30 210432.0 31 271326.0 32 359144.0 33 490735.0 34 691730.0 35 1118908.0 36 2597534.0 37 7545765.0 38 6908101.0 39 4833.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.253842766686994 11.927884222283733 9.482098532056 58.33617447897327 2 20.98510004987574 18.363203694377166 42.46234273659134 18.18935351915575 3 23.960617426056167 23.11189078560123 24.167358170134374 28.76013361820823 4 27.822712141757826 28.171411474213652 16.98829317635472 27.017583207673805 5 28.969576086133998 29.63892413333437 21.261918266997874 20.129581513533758 6 20.838457982066643 32.17894987524177 23.294442080043023 23.688150062648567 7 19.161996069823914 14.45607109951252 41.159814804756714 25.22211802590685 8 20.801930198851775 19.226016952643313 28.1464995323475 31.82555331615741 9 22.175810264367765 18.773180891699035 31.059453521218362 27.991555322714838 10-11 25.49987225362571 27.5516240233645 20.9833919875066 25.96511173550319 12-13 23.30223589205761 22.073642113758908 27.528213167282487 27.095908826900995 14-15 24.19801516649161 23.662214087277007 25.804248880761442 26.335521865469936 16-17 25.463705920006475 23.994059298768576 24.39182807144471 26.15040670978024 18-19 25.046607049454213 24.132875452342546 24.453894937517983 26.366622560685254 20-21 25.010719715485035 24.359223585484017 24.885644255486746 25.744412443544203 22-23 25.099383118893755 24.371533826357545 24.62681315973648 25.902269895012214 24-25 24.886863732943304 24.43917518876866 24.563740811717835 26.1102202665702 26-27 24.76308977547863 24.56054265117602 24.964399455533908 25.71196811781144 28-29 25.133659798838682 24.236997167310097 24.527857088569245 26.101485945281972 30-31 25.004156060201115 24.14511200465104 24.586947590722062 26.263784344425783 32-33 25.129181956714714 24.399242806689323 24.67812700373259 25.793448232863376 34-35 25.3418991670989 24.42840660803603 24.268669551190072 25.96102467367499 36-37 25.097605269283118 24.429472371444565 24.315886638495055 26.157035720777266 38-39 25.187527510889584 24.47318587133278 24.672730117701555 25.666556500076084 40-41 25.43768557698035 24.309429579271363 24.409253376742377 25.843631467005906 42-43 25.034688471277196 24.149347275473712 24.690478052135457 26.125486201113628 44-45 25.203947407891814 24.331935374282303 24.776652465217573 25.687464752608307 46-47 25.447887969931738 24.24591102333705 24.398707345366226 25.907493661364988 48-49 25.18410387414346 24.156322965925664 24.44745154278167 26.212121617149204 50-51 25.235600697471135 24.36461042213395 24.753701022372056 25.646087858022863 52-53 25.521031694092322 24.24913284203481 24.436056188490152 25.793779275382718 54-55 25.154574585752385 24.119819016891764 24.518789125754747 26.206817271601103 56-57 25.096291931994998 24.446880982621398 24.841512999290728 25.615314086092877 58-59 25.45655414790001 24.34774246456782 24.328038327955095 25.86766505957707 60-61 25.113905284990107 24.345547695472057 24.50203074353631 26.038516276001527 62-63 25.184903844752604 24.46691589322822 24.71904844754619 25.629131814472988 64-65 25.372418637466314 24.426236561195857 24.342819173092277 25.858525628245555 66-67 25.201000505014854 24.325467213736403 24.41408855506223 26.059443726186515 68-69 25.292677602759234 24.54995382789723 24.602613133369996 25.554755435973536 70-71 25.51363745809053 24.425930883861476 24.28128483912562 25.77914681892237 72-73 25.29263901504497 24.33624375981461 24.448473002197385 25.922644222943035 74-75 25.388533607284913 24.510005218646274 24.609916740788947 25.491544433279866 76-77 25.610619975814185 24.447520572092206 24.24267729853676 25.699182153556848 78-79 25.412014772275988 24.386531140272595 24.353973164416043 25.847480923035377 80-81 25.46473456058112 24.639851312317028 24.39109861504875 25.5043155120531 82-83 25.6550703769132 24.541165885763345 24.097614797991035 25.70614893933242 84-85 25.3694080974704 24.443457194296276 24.340090447602506 25.84704426063082 86-87 25.441475700375598 24.770561340523862 24.387517160824515 25.40044579827602 88-89 25.62316501631896 24.536129577507833 24.118470781273533 25.722234624899677 90-91 25.550895463177987 24.351228533406932 24.188131941282602 25.909744062132482 92-93 25.54180434899969 24.64651771481443 24.42825981902787 25.383418117158012 94-95 25.772099401490188 24.543644997811164 24.153572511992667 25.53068308870598 96-97 25.4407341356496 24.556390663127317 24.263206839632712 25.73966836159037 98-99 25.60726137130631 24.715859847242072 24.339883164713964 25.33699561673765 100-101 25.99085534270134 24.562393073111778 23.94531041950734 25.50144116467954 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 17110.0 1 9126.5 2 1043.0 3 901.0 4 803.5 5 684.5 6 600.5 7 541.0 8 495.0 9 469.0 10 456.0 11 476.0 12 513.0 13 546.5 14 612.0 15 701.0 16 789.0 17 911.5 18 1112.5 19 1365.0 20 1680.0 21 2096.0 22 2810.0 23 4067.0 24 5700.0 25 8087.5 26 11552.5 27 16188.0 28 22859.0 29 31844.5 30 43046.5 31 58081.5 32 77597.0 33 103410.0 34 138322.0 35 184030.0 36 240652.5 37 311726.0 38 395241.0 39 483678.0 40 576803.0 41 668660.0 42 749974.0 43 808044.0 44 842714.0 45 852128.5 46 841260.0 47 820783.0 48 785300.5 49 747167.5 50 713512.5 51 677725.0 52 650354.0 53 639750.5 54 642908.5 55 644674.5 56 640759.0 57 632400.5 58 611080.0 59 581006.0 60 557590.0 61 535857.0 62 509131.0 63 470001.5 64 414115.0 65 375607.0 66 355530.5 67 331713.5 68 294748.5 69 249810.5 70 211417.5 71 176858.5 72 144836.0 73 116237.5 74 92090.0 75 70620.5 76 49415.5 77 33857.0 78 23737.0 79 17269.0 80 11750.0 81 6973.0 82 4057.5 83 2425.5 84 1607.0 85 1087.5 86 828.5 87 661.5 88 539.0 89 422.5 90 330.0 91 282.0 92 231.0 93 191.0 94 140.5 95 90.0 96 59.5 97 37.5 98 19.0 99 10.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15514053882852838 2 0.14771165598739402 3 0.15652145907940088 4 0.15186377891120384 5 0.15799600104219688 6 0.10715941146770477 7 0.11034723075870193 8 0.10767901196888051 9 0.09533030816616318 10-11 0.08209453864297014 12-13 0.09074050373911073 14-15 0.1361680989072193 16-17 0.14547643761521906 18-19 0.13269473339485527 20-21 0.16402992037567024 22-23 0.14617625991184768 24-25 0.14247586174806903 26-27 0.13234131143234384 28-29 0.13059058541937332 30-31 0.09473581029544859 32-33 0.1062840484612195 34-35 0.12142267927925886 36-37 0.12704466308026854 38-39 0.12496860161836364 40-41 0.15908435344330826 42-43 0.13963678333398713 44-45 0.12945074107670398 46-47 0.16303050860088625 48-49 0.18576888188432045 50-51 0.16089593356902912 52-53 0.11049702549777964 54-55 0.1123460543082699 56-57 0.11449701313971364 58-59 0.1142512561459143 60-61 0.1190423472536024 62-63 0.10115357864780852 64-65 0.11837529255614705 66-67 0.13359818291491762 68-69 0.1322640735200069 70-71 0.12008622934155007 72-73 0.1294928708470696 74-75 0.1647554886430778 76-77 0.19373843011181333 78-79 0.16849333549162587 80-81 0.142000731560057 82-83 0.12547649940554895 84-85 0.13264090091049924 86-87 0.1259539701363591 88-89 0.1290434866298365 90-91 0.14384273874215298 92-93 0.11042680921383695 94-95 0.11579601439265302 96-97 0.11669478282711919 98-99 0.11985685614733735 100-101 0.11279309798270488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2.1362566E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.4112048721613 #Duplication Level Percentage of deduplicated Percentage of total 1 71.54914557723058 32.49132908235711 2 13.705530052297604 12.447692661729 3 5.299933789453297 7.220291373652615 4 2.7639321595428124 5.020539581990154 5 1.6450562608847092 3.735199344463358 6 1.076027540582308 2.9318224256082637 7 0.7434368571454122 2.363225440054222 8 0.5177794694327355 1.8810391652007146 9 0.3513089094971172 1.4358024776330245 >10 2.133366040059244 17.558950109744963 >50 0.14118004145303 4.3834215046409195 >100 0.0671912050254058 5.525924174421859 >500 0.004007366049403621 1.2715833512439132 >1k 0.0020383938892837563 1.4556314114028737 >5k 4.5596196608975345E-5 0.1335571825040404 >10k+ 2.07412604486634E-5 0.14399071335299615 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.07479906674132686 3.74486847694233E-5 0.0 2 0.07524376987296376 4.212977036560121E-5 1.4043256788533738E-5 3 0.07764048569820685 5.1491941557957034E-5 1.4043256788533738E-5 4 0.08591196394665322 5.1491941557957034E-5 1.4043256788533738E-5 5 0.0874005491662378 6.085411275031286E-5 1.4043256788533738E-5 6 0.09204418607764629 7.48973695388466E-5 1.4043256788533738E-5 7 0.09297104102568951 1.0298388311591407E-4 1.4043256788533738E-5 8 0.09543797313487527 1.0298388311591407E-4 1.4043256788533738E-5 9 0.09874281956577688 1.0766496871209198E-4 1.872434238471165E-5 10-11 0.10485163626878906 1.2638931109680364E-4 1.872434238471165E-5 12-13 0.11184985923507504 1.4745419627960424E-4 1.872434238471165E-5 14-15 0.11887616871493809 1.5681636747196006E-4 1.872434238471165E-5 16-17 0.12589311602360878 1.7554070985667172E-4 2.5745970778978517E-5 18-19 0.13239982500229608 1.872434238471165E-4 3.276759917324539E-5 20-21 0.13889249072419485 2.12989394626095E-4 3.5108141971334343E-5 22-23 0.14412828496351984 2.3405427980889562E-4 4.2129770365601215E-5 24-25 0.14872511101896654 2.4341645100125144E-4 5.383248435604599E-5 26-27 0.15293808805552667 2.527786221936073E-4 6.319465554840182E-5 28-29 0.15647932930903524 2.8086513577067475E-4 6.787574114457973E-5 30-31 0.15961331611567636 2.9724893535729743E-4 7.255682674075764E-5 32-33 0.16245473507255637 3.2299490613627594E-4 7.48973695388466E-5 34-35 0.16517444580393573 3.346976201267207E-4 9.12811691254693E-5 36-37 0.16765308062711193 3.74486847694233E-4 1.0766496871209198E-4 38-39 0.17022065607661552 3.7682739049232195E-4 1.0766496871209198E-4 40-41 0.17254247453231975 3.8618956168467777E-4 1.123460543082699E-4 42-43 0.17486195244522593 4.119355324636563E-4 1.240487682987147E-4 44-45 0.17725398718487284 4.587463884254354E-4 1.3107039669298155E-4 46-47 0.17948452447145158 4.657680168197023E-4 1.3107039669298155E-4 48-49 0.1816939968728476 4.8683290200250294E-4 1.3107039669298155E-4 50-51 0.18408837215529256 5.055572443872146E-4 1.3575148228915946E-4 52-53 0.18645466092416052 5.196005011757483E-4 1.3809202508724842E-4 54-55 0.18887478217738451 5.40665386358549E-4 1.4277311068342633E-4 56-57 0.19121532497547344 5.476870147528158E-4 1.4745419627960424E-4 58-59 0.19377821933938086 5.570491859451716E-4 1.59156910270049E-4 60-61 0.1965424003839239 5.640708143394384E-4 1.6383799586622692E-4 62-63 0.19943999236795806 5.991789563107728E-4 1.6617853866431588E-4 64-65 0.2027822874836291 6.179032986954844E-4 1.6851908146240483E-4 66-67 0.2060824528289345 6.249249270897513E-4 1.6851908146240483E-4 68-69 0.20958390485487557 6.342870982821072E-4 1.6851908146240483E-4 70-71 0.21360027629639622 6.717357830515304E-4 1.6851908146240483E-4 72-73 0.21837264306169962 6.834384970419753E-4 1.7320016705858277E-4 74-75 0.22404845934706535 6.97481753830509E-4 1.7788125265476068E-4 76-77 0.23064410895208 7.138655534171317E-4 1.8022179545284963E-4 78-79 0.2391870901651047 7.162060962152206E-4 1.872434238471165E-4 80-81 0.2500589114622279 7.372709813980212E-4 1.919245094432944E-4 82-83 0.2644883578124463 7.48973695388466E-4 2.0596776623182814E-4 84-85 0.28272352675235735 7.536547809846439E-4 2.0596776623182814E-4 86-87 0.30556254337610944 7.630169521769997E-4 2.0596776623182814E-4 88-89 0.3342810035086609 8.051467225426009E-4 2.0596776623182814E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 2065 0.0 15.872457 1 CGAATTA 7795 0.0 14.657964 14-15 CTCGTAT 5675 0.0 13.392733 1 CTCGGTT 9475 0.0 12.784274 1 CTCGGAT 11570 0.0 12.152719 1 CTCGGGT 12450 0.0 11.44635 1 TTAGGTG 10230 0.0 11.262062 18-19 CTCGATT 12070 0.0 11.255734 1 CTCGTTT 9520 0.0 11.177024 1 CTGGAAT 18755 0.0 11.169575 1 CTCGACT 10655 0.0 11.100971 1 GAGTGTC 10755 0.0 10.930849 24-25 AATTAGG 10800 0.0 10.843662 16-17 CTCGCAT 7270 0.0 10.650458 1 CTCGAAT 9515 0.0 10.53389 1 CTCAGAT 16985 0.0 10.375844 1 CGCGAAT 2660 0.0 10.357656 1 GTCGAAT 11435 0.0 10.301333 12-13 CGGGTCG 14650 0.0 10.179816 9 CTCAGTT 12060 0.0 10.04403 1 >>END_MODULE