FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 mx12-2.3420000000cd81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 mx12-2.3420000000cd81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21362566
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT20650.015.8724571
CGAATTA77950.014.65796414-15
CTCGTAT56750.013.3927331
CTCGGTT94750.012.7842741
CTCGGAT115700.012.1527191
CTCGGGT124500.011.446351
TTAGGTG102300.011.26206218-19
CTCGATT120700.011.2557341
CTCGTTT95200.011.1770241
CTGGAAT187550.011.1695751
CTCGACT106550.011.1009711
GAGTGTC107550.010.93084924-25
AATTAGG108000.010.84366216-17
CTCGCAT72700.010.6504581
CTCGAAT95150.010.533891
CTCAGAT169850.010.3758441
CGCGAAT26600.010.3576561
GTCGAAT114350.010.30133312-13
CGGGTCG146500.010.1798169
CTCAGTT120600.010.044031
Produced by FastQC (version 0.11.2)