##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 m48-1.3420000000cb40.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15396687 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.439899895347615 33.0 33.0 33.0 33.0 33.0 2 32.415438529080966 33.0 33.0 33.0 33.0 33.0 3 32.404027372901716 33.0 33.0 33.0 33.0 33.0 4 36.27057314343014 37.0 37.0 37.0 37.0 37.0 5 36.264455463698134 37.0 37.0 37.0 37.0 37.0 6 36.28704973998627 37.0 37.0 37.0 37.0 37.0 7 36.309275365538056 37.0 37.0 37.0 37.0 37.0 8 36.319427809372236 37.0 37.0 37.0 37.0 37.0 9 36.29727362776161 37.0 37.0 37.0 37.0 37.0 10-11 36.279653051335 37.0 37.0 37.0 37.0 37.0 12-13 36.267387523043105 37.0 37.0 37.0 37.0 37.0 14-15 38.579998995887884 40.0 40.0 40.0 37.0 40.0 16-17 38.56008006787434 40.0 40.0 40.0 37.0 40.0 18-19 38.53226710395555 40.0 40.0 40.0 37.0 40.0 20-21 38.49428831670086 40.0 40.0 40.0 37.0 40.0 22-23 38.49499629368318 40.0 40.0 40.0 37.0 40.0 24-25 38.431839330110435 40.0 40.0 40.0 37.0 40.0 26-27 38.39067761785377 40.0 38.5 40.0 37.0 40.0 28-29 38.32856347602572 40.0 37.0 40.0 37.0 40.0 30-31 38.26334389339733 40.0 37.0 40.0 37.0 40.0 32-33 38.18083279864038 40.0 37.0 40.0 37.0 40.0 34-35 38.106950378350874 40.0 37.0 40.0 37.0 40.0 36-37 38.02609168452928 40.0 37.0 40.0 37.0 40.0 38-39 37.91897971297332 40.0 37.0 40.0 35.0 40.0 40-41 37.812552174373614 40.0 37.0 40.0 33.0 40.0 42-43 37.69762413823182 40.0 37.0 40.0 33.0 40.0 44-45 37.594402191848154 40.0 37.0 40.0 33.0 40.0 46-47 37.43676928679527 40.0 37.0 40.0 33.0 40.0 48-49 37.306092473010594 40.0 37.0 40.0 33.0 40.0 50-51 35.85100872674751 38.5 35.0 38.5 30.0 38.5 52-53 36.198993296415 37.0 37.0 38.5 33.0 40.0 54-55 36.8824422747569 37.0 37.0 40.0 33.0 40.0 56-57 36.8419292410114 37.0 37.0 40.0 33.0 40.0 58-59 36.68589768695045 37.0 37.0 40.0 33.0 40.0 60-61 36.52898305330231 37.0 37.0 40.0 33.0 40.0 62-63 36.34620785627453 37.0 37.0 40.0 33.0 40.0 64-65 36.15238294446071 37.0 37.0 40.0 33.0 40.0 66-67 35.89635517043374 37.0 37.0 37.0 33.0 40.0 68-69 35.66590572374433 37.0 37.0 37.0 33.0 40.0 70-71 35.46559617013712 37.0 37.0 37.0 33.0 40.0 72-73 35.25773242646291 37.0 37.0 37.0 33.0 40.0 74-75 35.085742471740836 37.0 37.0 37.0 33.0 37.0 76-77 34.90744999232627 37.0 35.0 37.0 33.0 37.0 78-79 34.75998960036013 37.0 33.0 37.0 33.0 37.0 80-81 34.65483025666496 37.0 33.0 37.0 33.0 37.0 82-83 34.566457511281484 37.0 33.0 37.0 30.0 37.0 84-85 34.46765891909084 37.0 33.0 37.0 27.0 37.0 86-87 34.38006699103515 37.0 33.0 37.0 27.0 37.0 88-89 34.293575299673236 37.0 33.0 37.0 27.0 37.0 90-91 34.19369199360875 37.0 33.0 37.0 27.0 37.0 92-93 34.14245623100606 37.0 33.0 37.0 27.0 37.0 94-95 34.0743667452615 37.0 33.0 37.0 27.0 37.0 96-97 34.00998692121233 37.0 33.0 37.0 27.0 37.0 98-99 33.92618840663579 37.0 33.0 37.0 27.0 37.0 100-101 32.17911405226332 35.0 30.0 37.0 21.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1359423973593863 2306 2 0.25512435215859597 2306 3 0.2634097461363254 2306 4 0.3562368557433544 2306 5 0.34730800945910545 2306 6 0.38622703606164066 2306 7 0.38171966868170415 2306 8 0.35173725557163493 2306 9 0.3517343164378275 2306 10-11 0.3715022818428295 2306 12-13 0.3594995707535773 2306 14-15 0.6192907222850437 2306 16-17 0.581373840338685 2306 18-19 0.6362899976227467 2306 20-21 0.6877027455797915 2306 22-23 0.6709671114558162 2306 24-25 0.6718125207533276 2306 26-27 0.7194006301717764 2306 28-29 0.7382255489541762 2306 30-31 0.7267520468012947 2306 32-33 0.6448368122171999 2306 34-35 0.7121356680200037 2306 36-37 0.7144462330944137 2306 38-39 0.7460548897754009 2306 40-41 0.7541659273551531 2306 42-43 0.7669889165430916 2306 44-45 0.7667303105777776 2306 46-47 0.7642331823441424 2306 48-49 0.7544172790447021 2306 50-51 0.6295700378082074 2306 52-53 0.6758889607337224 2306 54-55 0.7458074804400852 2306 56-57 0.6628148921951507 2306 58-59 0.7377287896961846 2306 60-61 0.6914495729782786 2306 62-63 0.6865810819046345 2306 64-65 0.7090710347450795 2306 66-67 0.7451935670304763 2306 68-69 0.7788859329134468 2306 70-71 0.7539730632684396 2306 72-73 0.8083359544730158 2306 74-75 0.7663197114263269 2306 76-77 0.7394497546001446 2306 78-79 0.7701505424551343 2306 80-81 0.7856649412739642 2306 82-83 0.8053672246962407 2306 84-85 0.7960430739183195 2306 86-87 0.8031567401056847 2306 88-89 0.8349706602530702 2306 90-91 0.9094697837629226 2306 92-93 0.877913563809436 2306 94-95 0.87092751993098 2306 96-97 0.8420968925148742 2306 98-99 0.8376273358985244 2306 100-101 0.8024821101633179 2307 1 -0.03937541890648788 2307 2 0.1187098968187783 2307 3 0.21131933911394185 2307 4 0.2926974498675605 2307 5 0.30225918689716735 2307 6 0.31970391227974915 2307 7 0.3375104750762006 2307 8 0.35209573020658524 2307 9 0.34954036495445706 2307 10-11 0.3686339746605469 2307 12-13 0.36590697009852846 2307 14-15 0.6220302285490362 2307 16-17 0.6146114274052294 2307 18-19 0.6179926794491521 2307 20-21 0.6169442395225175 2307 22-23 0.6194005160887457 2307 24-25 0.6283710953939092 2307 26-27 0.6306660430826554 2307 28-29 0.6844742477142063 2307 30-31 0.690767748270865 2307 32-33 0.6377010500556182 2307 34-35 0.6652021437042421 2307 36-37 0.6750191880387035 2307 38-39 0.7023291459532857 2307 40-41 0.7000929426336242 2307 42-43 0.6889361339267737 2307 44-45 0.7089542356926657 2307 46-47 0.6990594075883863 2307 48-49 0.732116675299963 2307 50-51 0.6901309543300371 2307 52-53 0.7124565616406358 2307 54-55 0.7156312106447587 2307 56-57 0.7244996923732785 2307 58-59 0.7412567871262894 2307 60-61 0.7194357170600938 2307 62-63 0.7157159771467718 2307 64-65 0.687997419154101 2307 66-67 0.7200001711764088 2307 68-69 0.7510296099552178 2307 70-71 0.7017967677129278 2307 72-73 0.801708382073187 2307 74-75 0.7930337870329751 2307 76-77 0.7814955391182536 2307 78-79 0.8622166736925507 2307 80-81 0.8495320881002542 2307 82-83 0.8142306407592983 2307 84-85 0.8029325202643847 2307 86-87 0.7878211416615528 2307 88-89 0.8195978243157285 2307 90-91 0.8390146612547582 2307 92-93 0.8596235783613935 2307 94-95 0.8737420112840297 2307 96-97 0.8748905627531514 2307 98-99 0.8451925407257832 2307 100-101 0.7889327636621957 2201 1 0.14889969293381 2201 2 0.2275188486280939 2201 3 0.2416517731392105 2201 4 0.3017503994961146 2201 5 0.2835190376748642 2201 6 0.31434107671640277 2201 7 0.30985133831003964 2201 8 0.26910144195924346 2201 9 0.2906070258465334 2201 10-11 0.269795591529828 2201 12-13 0.2879155075807347 2201 14-15 0.5061889572722009 2201 16-17 0.5221056719185171 2201 18-19 0.49764664116020185 2201 20-21 0.5570638245670025 2201 22-23 0.5729798407500013 2201 24-25 0.5782388104890401 2201 26-27 0.5465696990799103 2201 28-29 0.5312496465857848 2201 30-31 0.5627652779335861 2201 32-33 0.6016699198996704 2201 34-35 0.5825613901027609 2201 36-37 0.6228857516118822 2201 38-39 0.6020467428390788 2201 40-41 0.6264904367995854 2201 42-43 0.6051813826249415 2201 44-45 0.5528315102881933 2201 46-47 0.5446305276557766 2201 48-49 0.6036927869942019 2201 50-51 0.5498641596032598 2201 52-53 0.6376963771253799 2201 54-55 0.6435598744546951 2201 56-57 0.645080291691194 2201 58-59 0.6280527749724669 2201 60-61 0.6313124430692056 2201 62-63 0.676484087292053 2201 64-65 0.6505356439930026 2201 66-67 0.6466812467284768 2201 68-69 0.7041596521097517 2201 70-71 0.6973761250685726 2201 72-73 0.7185656952565864 2201 74-75 0.6926586613506416 2201 76-77 0.7651882029214008 2201 78-79 0.737156008612196 2201 80-81 0.746829928487081 2201 82-83 0.7096922876904443 2201 84-85 0.7418473488268873 2201 86-87 0.7429332282261143 2201 88-89 0.7038773524464901 2201 90-91 0.6969761595750299 2201 92-93 0.7303941660621831 2201 94-95 0.7331660528713044 2201 96-97 0.7813373129306385 2201 98-99 0.8043433930728128 2201 100-101 0.6973414427068789 1101 1 -0.018885203570853548 1101 2 0.14979584081855535 1101 3 0.18064983339144192 1101 4 0.27253400704786657 1101 5 0.2901410016852779 1101 6 0.3363353838410319 1101 7 0.28079995079013287 1101 8 0.31231301978925075 1101 9 0.28760016406777567 1101 10-11 0.27112439773196684 1101 12-13 0.3172768282519982 1101 14-15 0.4853199407938291 1101 16-17 0.5198504624004912 1101 18-19 0.5682027548407902 1101 20-21 0.5582796104491692 1101 22-23 0.5767212951185385 1101 24-25 0.5861092896762372 1101 26-27 0.6059599738037988 1101 28-29 0.5814678840058605 1101 30-31 0.6106083984633273 1101 32-33 0.5780730462423307 1101 34-35 0.6116779676800306 1101 36-37 0.5676236961110916 1101 38-39 0.6417796642129545 1101 40-41 0.6572863652247918 1101 42-43 0.6340362996012132 1101 44-45 0.6202425472634445 1101 46-47 0.6354928308377197 1101 48-49 0.6214695011752127 1101 50-51 0.5680399753082668 1101 52-53 0.6190953965102253 1101 54-55 0.6774415972287215 1101 56-57 0.6671851865530769 1101 58-59 0.6510788130850358 1101 60-61 0.6707669127029874 1101 62-63 0.649613368005781 1101 64-65 0.6865399057515447 1101 66-67 0.7149772225416555 1101 68-69 0.7057652198808739 1101 70-71 0.6592359767126155 1101 72-73 0.7206162677033205 1101 74-75 0.7126745995061725 1101 76-77 0.7331680693504978 1101 78-79 0.7391684337392022 1101 80-81 0.7464625295182401 1101 82-83 0.7837662792743387 1101 84-85 0.814686283418844 1101 86-87 0.7635277261663163 1101 88-89 0.7262209247415896 1101 90-91 0.7838558667805273 1101 92-93 0.784168539277232 1101 94-95 0.8194228293517014 1101 96-97 0.7487057797085157 1101 98-99 0.7322085806028227 1101 100-101 0.7596483209134632 2304 1 0.011176733426765395 2304 2 0.17049189689594613 2304 3 0.21378375617572942 2304 4 0.27586501049496803 2304 5 0.2761818680950441 2304 6 0.2968422110748321 2304 7 0.2751120159571556 2304 8 0.2926508789522515 2304 9 0.2929334430028874 2304 10-11 0.3078785480342674 2304 12-13 0.3430204571165163 2304 14-15 0.5294022401759975 2304 16-17 0.5666705217971924 2304 18-19 0.5833761260886661 2304 20-21 0.5924944670149088 2304 22-23 0.598324336389858 2304 24-25 0.6266355078244104 2304 26-27 0.5449788812924297 2304 28-29 0.6212764458758855 2304 30-31 0.6005619649597733 2304 32-33 0.6116805030072854 2304 34-35 0.6228125863510385 2304 36-37 0.6239491909681476 2304 38-39 0.6763174306936932 2304 40-41 0.6769543333433248 2304 42-43 0.668278090986071 2304 44-45 0.6752631390442829 2304 46-47 0.6798390745120031 2304 48-49 0.7060863323965094 2304 50-51 0.6239739107170479 2304 52-53 0.6219285638263301 2304 54-55 0.6832460007137016 2304 56-57 0.6819925706502161 2304 58-59 0.7042021897983872 2304 60-61 0.662154221391873 2304 62-63 0.6909902229980744 2304 64-65 0.7075294043282057 2304 66-67 0.7245488057301444 2304 68-69 0.7668208199984932 2304 70-71 0.761962336193541 2304 72-73 0.754465799134465 2304 74-75 0.8169761032065352 2304 76-77 0.8213757915377329 2304 78-79 0.8018496529573795 2304 80-81 0.8311354627577643 2304 82-83 0.7902507118880209 2304 84-85 0.8098213578032798 2304 86-87 0.8145620109040692 2304 88-89 0.7870331569522122 2304 90-91 0.7888492312234945 2304 92-93 0.8457169488219662 2304 94-95 0.7994659296810127 2304 96-97 0.7693466806046985 2304 98-99 0.8198886759331501 2304 100-101 0.8724770080738864 2202 1 0.1391760297294553 2202 2 0.16038446607667112 2202 3 0.18212338681654927 2202 4 0.2099941319379255 2202 5 0.24244820430308067 2202 6 0.2514782815421128 2202 7 0.2108861181990136 2202 8 0.2552073988585377 2202 9 0.24522012598904297 2202 10-11 0.2379677407581653 2202 12-13 0.28791022748895756 2202 14-15 0.44015466707912765 2202 16-17 0.47955429120130333 2202 18-19 0.4450202754496999 2202 20-21 0.43036024312400656 2202 22-23 0.43647226867269495 2202 24-25 0.4996805614504112 2202 26-27 0.46011159507695965 2202 28-29 0.4842664150037308 2202 30-31 0.49313429771126494 2202 32-33 0.4909097437827228 2202 34-35 0.5273599531597668 2202 36-37 0.4843031995948124 2202 38-39 0.5422219160975033 2202 40-41 0.5359311090567545 2202 42-43 0.5106200062339639 2202 44-45 0.5450367215973202 2202 46-47 0.5234776286121203 2202 48-49 0.5840488818762282 2202 50-51 0.5317357712811699 2202 52-53 0.5412724260468522 2202 54-55 0.5843147798178734 2202 56-57 0.588908732295117 2202 58-59 0.5444206392545823 2202 60-61 0.5743578165388072 2202 62-63 0.5891118613932207 2202 64-65 0.5729809488337807 2202 66-67 0.5871030462760416 2202 68-69 0.5672230193631052 2202 70-71 0.5895240916688422 2202 72-73 0.6383802029548349 2202 74-75 0.6282603344682158 2202 76-77 0.6318136546610589 2202 78-79 0.6279841513887376 2202 80-81 0.6188910733504969 2202 82-83 0.57932750159884 2202 84-85 0.6442722759465127 2202 86-87 0.6202010173427865 2202 88-89 0.6623455718693663 2202 90-91 0.652635905737327 2202 92-93 0.647033649525568 2202 94-95 0.6684471166193262 2202 96-97 0.6435276935674423 2202 98-99 0.639893314424846 2202 100-101 0.7883550780598085 1102 1 0.09115983519647841 1102 2 0.15237521952150956 1102 3 0.14978522502476466 1102 4 0.20759622852982318 1102 5 0.2483267041710775 1102 6 0.2496979952057572 1102 7 0.2445544844094485 1102 8 0.2105836330982953 1102 9 0.25448096599577497 1102 10-11 0.21538428331650294 1102 12-13 0.22970543755683792 1102 14-15 0.35796873018575326 1102 16-17 0.40470513164597577 1102 18-19 0.4252837838321639 1102 20-21 0.3881151934547731 1102 22-23 0.3849866567373468 1102 24-25 0.40299248800658205 1102 26-27 0.42056677686711197 1102 28-29 0.424741624534974 1102 30-31 0.46149109031997426 1102 32-33 0.46037089016005694 1102 34-35 0.46936117348850104 1102 36-37 0.4189583902383731 1102 38-39 0.4483847269103194 1102 40-41 0.5128232613229002 1102 42-43 0.5297761590802565 1102 44-45 0.5093306906804784 1102 46-47 0.5331724335448058 1102 48-49 0.5466205255097378 1102 50-51 0.4613041302280294 1102 52-53 0.5054859727005194 1102 54-55 0.5490390968123293 1102 56-57 0.5331923213736545 1102 58-59 0.5308410423850489 1102 60-61 0.566352739597761 1102 62-63 0.582331058213839 1102 64-65 0.591122744791484 1102 66-67 0.5948027100650677 1102 68-69 0.5661542358602887 1102 70-71 0.5657152877724414 1102 72-73 0.6250957385232994 1102 74-75 0.6304821123124782 1102 76-77 0.6482011600976634 1102 78-79 0.6688605215325012 1102 80-81 0.6202292151068605 1102 82-83 0.6751752278825478 1102 84-85 0.6542035698076134 1102 86-87 0.6614574724875766 1102 88-89 0.6480745164558144 1102 90-91 0.6805284196458459 1102 92-93 0.6905509575021256 1102 94-95 0.7230470904609803 1102 96-97 0.7541237054778236 1102 98-99 0.6680204851138427 1102 100-101 0.6583258127469946 2305 1 0.13412339500327164 2305 2 0.12754788973975195 2305 3 0.14434462753975907 2305 4 0.1663713058335361 2305 5 0.2056023767326991 2305 6 0.20831619512294708 2305 7 0.21568688440171968 2305 8 0.22618124065142098 2305 9 0.22810223157652842 2305 10-11 0.22727064021671595 2305 12-13 0.20265909654454362 2305 14-15 0.31734764992295794 2305 16-17 0.32653660840784937 2305 18-19 0.38707187807346344 2305 20-21 0.3927244778356638 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-0.899523306024463 1213 78-79 -0.8573365441551388 1213 80-81 -0.9392335475810327 1213 82-83 -0.9253560467606547 1213 84-85 -0.9939668269309792 1213 86-87 -0.9571722020375191 1213 88-89 -0.9968444821288145 1213 90-91 -0.9974474185573712 1213 92-93 -0.977559722282713 1213 94-95 -1.05573816933056 1213 96-97 -1.1504280473809132 1213 98-99 -1.0295634098876008 1213 100-101 -0.9823976849122396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13855.0 3 1489.0 4 888.0 5 729.0 6 620.0 7 810.0 8 1469.0 9 4046.0 10 14510.0 11 24183.0 12 20478.0 13 18933.0 14 19880.0 15 21049.0 16 23124.0 17 26269.0 18 29234.0 19 32165.0 20 35986.0 21 40177.0 22 44573.0 23 50517.0 24 57362.0 25 66121.0 26 78362.0 27 102295.0 28 112763.0 29 124889.0 30 155012.0 31 198821.0 32 261117.0 33 357390.0 34 511188.0 35 830445.0 36 1921430.0 37 5497188.0 38 4693965.0 39 3355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 22.915683483412497 12.808951006592096 15.977600887367677 48.29776462262773 2 24.331771811086984 18.421908125461727 34.81396308153977 22.432356981911518 3 26.59931076410369 22.93735639563634 24.042927044787586 26.42040579547239 4 28.312556586169986 27.19847047651645 17.981657868836454 26.507315068477112 5 29.642650793673507 29.03287478434164 20.952898691667183 20.371575730317666 6 22.907695066434215 32.772876109572145 21.54258144642839 22.77684737756525 7 20.656555158236657 15.989276485947723 39.79452449809438 23.55964385772124 8 22.547562825763762 21.168371889562778 26.170356884225466 30.113708400447997 9 22.97100659261104 20.79790038122583 29.413333744197278 26.81775928196585 10-11 25.756238789224916 28.254458553079964 20.59399598094018 25.39530667675494 12-13 24.060552441667042 22.55046629994279 26.951396588830235 26.437584669559932 14-15 24.5012127267172 24.310979108218252 25.79087705889415 25.396931106170396 16-17 25.454143614642582 24.309684827403036 24.822542464066615 25.413629093887767 18-19 25.30754988859379 24.654359793585208 25.03332821517043 25.00476210265058 20-21 25.043569172564823 24.794642898672933 25.13642739973447 25.025360529027772 22-23 25.346695662080133 24.552155466912325 24.80831471850467 25.29283415250287 24-25 25.11292479336728 24.85653732595351 24.72386559195961 25.306672288719607 26-27 24.89127183806149 25.053000812725735 24.763477430813772 25.29224991839901 28-29 25.26537537024698 24.82282187213572 24.457618175005127 25.45418458261218 30-31 25.38339123027371 24.422267673269804 24.813462484287605 25.38087861216888 32-33 25.178523902462207 24.695699500479286 24.757852296622048 25.367924300436457 34-35 25.107904492814985 24.85273647146334 24.336791875902268 25.702567159819406 36-37 24.799361066451702 24.734412273413252 24.773397806756485 25.692828853378565 38-39 24.89631414282668 24.607014368518605 24.869060605825126 25.627610882829593 40-41 25.58227368700977 24.407292151702297 24.44611776993611 25.56431639135182 42-43 25.27406270732948 24.507178164970224 24.952910740903743 25.265848386796552 44-45 25.545571153356917 24.399379440695434 24.715229065357985 25.339820340589668 46-47 25.27156706543114 24.385969642932825 24.630893193406074 25.71157009822996 48-49 24.95151509650278 24.654243612894174 24.519672405341502 25.874568885261546 50-51 24.979181749232662 24.5111947578015 24.945016464323654 25.56460702864218 52-53 25.147368970980104 24.655825348974712 24.78910542272755 25.407700257317632 54-55 24.958795427559554 24.603065951146075 24.74876808918686 25.689370532107514 56-57 24.72648714022374 24.624691550683224 25.164034890941423 25.48478641815161 58-59 25.13677375962098 24.735240202941437 24.6868215767937 25.44116446064388 60-61 25.01500940294471 24.696526551378586 24.728668424553373 25.559795621123328 62-63 24.901478293692985 24.774253054764518 24.808297459877142 25.515971191665354 64-65 25.081866654329353 24.86190178641475 24.3558583934222 25.700373165833696 66-67 24.956461223385805 24.85593784758752 24.429632108637303 25.757968820389372 68-69 25.04483400881332 24.791828166214902 24.578427649069504 25.584910175902277 70-71 25.21078696574972 24.773795465895375 24.389113805193723 25.62630376316118 72-73 25.048630642273046 24.858370895594604 24.659159547005373 25.433838915126977 74-75 25.402517087583742 24.699311838714145 24.530663321019404 25.367507752682712 76-77 25.611455272806 24.66773493681661 24.34565958827338 25.375150202104006 78-79 25.205016664846696 24.695197232916243 24.459697282473435 25.640088819763623 80-81 25.443103450630744 24.791956187760753 24.307255031415103 25.457685330193396 82-83 25.390156663534214 24.71335339220677 24.231588622328143 25.664901321930873 84-85 25.286725196510528 24.62124894897506 24.267127763856607 25.824898090657804 86-87 25.260189097666476 24.95984760232082 24.324464928413693 25.45549837159901 88-89 25.45800541938979 24.873256285241926 24.2784533002367 25.39028499513159 90-91 25.549077779834512 24.637189347199687 24.3102324415501 25.5035004314157 92-93 25.43366914857199 24.86721215917961 24.54824565823699 25.15087303401141 94-95 25.38327638902873 24.708781339429787 24.400251157268592 25.507691114272884 96-97 25.236469494692294 24.923976098645916 24.280932268551137 25.558622138110653 98-99 25.481196774828703 24.574536763326954 24.517080043790916 25.427186418053427 100-101 25.51578975156349 24.6976400980946 24.366647380350525 25.419922769991388 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12185.0 1 6484.0 2 724.0 3 651.5 4 570.5 5 491.0 6 440.5 7 350.0 8 329.0 9 360.0 10 377.0 11 406.5 12 423.0 13 450.5 14 503.0 15 587.0 16 682.5 17 782.5 18 958.0 19 1150.0 20 1418.5 21 1870.0 22 2489.0 23 3364.0 24 4540.5 25 6457.0 26 9155.0 27 12629.5 28 17527.5 29 24335.0 30 33222.0 31 44510.5 32 59001.5 33 78198.5 34 103749.5 35 138776.0 36 182331.5 37 241120.5 38 303987.5 39 358646.5 40 415991.0 41 472560.0 42 524883.5 43 571723.5 44 612072.0 45 623476.5 46 612605.5 47 620622.0 48 605336.0 49 574090.5 50 575784.0 51 551588.0 52 510812.5 53 486545.0 54 469420.0 55 467151.0 56 478572.0 57 483654.0 58 458106.5 59 398836.5 60 357284.5 61 329710.5 62 293793.0 63 271695.0 64 240605.5 65 216569.5 66 213334.5 67 209523.0 68 193249.0 69 167008.0 70 142908.0 71 128139.5 72 105379.0 73 81497.0 74 75399.0 75 63070.5 76 40820.0 77 26560.0 78 20329.5 79 18456.5 80 15540.0 81 10820.5 82 5127.0 83 1525.0 84 965.5 85 699.5 86 524.5 87 411.0 88 351.5 89 314.0 90 281.0 91 231.0 92 176.0 93 140.0 94 109.5 95 65.0 96 39.0 97 26.5 98 16.5 99 11.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15313684041248612 2 0.14531048140421376 3 0.15406561164749275 4 0.14944773508742498 5 0.15583222546512765 6 0.10527589474281059 7 0.10816612690769124 8 0.10582146665708018 9 0.0939552775217162 10-11 0.08113433753638039 12-13 0.08913281149379733 14-15 0.1337495527446911 16-17 0.14257937438099508 18-19 0.1303916875104365 20-21 0.16150552388315745 22-23 0.14335226792621036 24-25 0.13977357596475137 26-27 0.13013189136078432 28-29 0.128527650136682 30-31 0.09304274354606287 32-33 0.10420098817362462 34-35 0.11943153744698454 36-37 0.12462421298815778 38-39 0.12253610143532825 40-41 0.1565596546841538 42-43 0.13704246894153269 44-45 0.12724165919590363 46-47 0.1607326303379422 48-49 0.183438813817544 50-51 0.15820286533070393 52-53 0.10826030301194017 54-55 0.11055949893636209 56-57 0.11260214616300246 58-59 0.11219946213104157 60-61 0.11686929792103977 62-63 0.09940450176067098 64-65 0.11604769259776469 66-67 0.13096323903967133 68-69 0.12968374300263427 70-71 0.11773312011863332 72-73 0.12698186304625145 74-75 0.16284347405386626 76-77 0.1914405352268316 78-79 0.16599999727214043 80-81 0.13925073621357634 82-83 0.12310440551269244 84-85 0.13009292193833646 86-87 0.12356554367832509 88-89 0.12661490098486772 90-91 0.14110178377984822 92-93 0.10847788228727387 94-95 0.11352117504239712 96-97 0.11458309180410045 98-99 0.11791497742338986 100-101 0.1106958919149295 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5396687E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.954588755324416 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9485036659983 31.445062163552564 2 17.03387251828062 15.315014679398553 3 5.595404239680063 7.546172895438478 4 2.344602692075346 4.216025992674948 5 1.3659555108658803 3.070298412452236 6 0.8993673199238639 2.4258412804293354 7 0.6001643999744678 1.8886100650506765 8 0.39895260086417583 1.4347800083772888 9 0.3222054462909793 1.303615199946307 >10 1.3094945522243415 10.430324950130093 >50 0.08513066360482525 2.652422739565979 >100 0.07665649350121302 7.291014792100668 >500 0.011425824214027302 3.6715036883096603 >1k 0.007980186013818802 6.190254901964866 >5k 2.402360982093714E-4 0.7304338266476484 >10k+ 4.3650389801674964E-5 0.3886244039606985 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC 33237 0.21587111564974984 Illumina Single End PCR Primer 1 (100% over 50bp) >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.22733462075315294 1.2989807482609734E-5 1.2989807482609734E-5 2 0.227548952576616 1.2989807482609734E-5 1.94847112239146E-5 3 0.227698335362666 1.94847112239146E-5 1.94847112239146E-5 4 0.2282374123731943 1.94847112239146E-5 3.2474518706524335E-5 5 0.22838679515924432 1.94847112239146E-5 3.2474518706524335E-5 6 0.22873751996127478 1.94847112239146E-5 4.5464326189134064E-5 7 0.22893236707351394 1.94847112239146E-5 4.5464326189134064E-5 8 0.22921164793439003 1.94847112239146E-5 4.5464326189134064E-5 9 0.22947144408404224 2.5979614965219467E-5 4.5464326189134064E-5 10-11 0.22997155167212271 6.494903741304867E-5 5.1959229930438935E-5 12-13 0.23088408564777604 8.76812005076157E-5 5.1959229930438935E-5 14-15 0.23169594861543916 1.1366081547283517E-4 5.1959229930438935E-5 16-17 0.23238765586388813 1.266506229554449E-4 5.1959229930438935E-5 18-19 0.23296894974873492 1.2989807482609734E-4 5.520668180109137E-5 20-21 0.23850910264006797 1.363929785674022E-4 5.84541336717438E-5 22-23 0.24304579290336942 1.4288788230870707E-4 5.84541336717438E-5 24-25 0.24353291068396726 1.753624010152314E-4 6.494903741304867E-5 26-27 0.24400379120521187 1.753624010152314E-4 6.81964892837011E-5 28-29 0.24446492937084452 1.8185730475653625E-4 7.144394115435353E-5 30-31 0.24487086085467608 2.0783691972175574E-4 7.79388448956584E-5 32-33 0.24535797863527392 2.143318234630606E-4 1.0067100799022544E-4 34-35 0.2458386015121305 2.1757927533371305E-4 1.266506229554449E-4 36-37 0.2462802549665392 2.6304360152284707E-4 1.363929785674022E-4 38-39 0.24677062019900775 2.662910533934995E-4 1.363929785674022E-4 40-41 0.24732268701701865 2.7928086087610925E-4 1.3964043043805462E-4 42-43 0.24775459811581546 2.8577576461741414E-4 1.4938278605001193E-4 44-45 0.24838135632685135 3.020130239706763E-4 1.558776897913168E-4 46-47 0.24897239256731007 3.0850792771198115E-4 1.6237259353262166E-4 48-49 0.2495861609708634 3.117553795826336E-4 1.6886749727392653E-4 50-51 0.2502843631230537 3.4422989828915794E-4 1.753624010152314E-4 52-53 0.2509241111415722 3.4422989828915794E-4 1.786098528858838E-4 54-55 0.2516060760344092 3.539722539011152E-4 1.8185730475653625E-4 56-57 0.25247964058761474 3.604671576424201E-4 1.8835220849784112E-4 58-59 0.25331748317024305 3.6696206138372495E-4 1.9159966036849354E-4 60-61 0.25425924421273227 3.767044169956823E-4 1.9484711223914598E-4 62-63 0.2553990998193313 3.929416763489444E-4 2.0134201598045085E-4 64-65 0.25654220287780094 4.1242638757285903E-4 2.0134201598045085E-4 66-67 0.25771778045497706 4.189212913141639E-4 2.2082672720436547E-4 68-69 0.2591726388930294 4.2541619505546876E-4 2.338165346869752E-4 70-71 0.2605852804567632 4.3515855066742604E-4 2.338165346869752E-4 72-73 0.2623194197556916 4.3515855066742604E-4 2.468063421695849E-4 74-75 0.2641899520331874 4.513958100206882E-4 2.533012459108898E-4 76-77 0.2662033721929919 4.7412797311525523E-4 2.5654869778154223E-4 78-79 0.2684343716281301 4.7412797311525523E-4 2.662910533934995E-4 80-81 0.2709933637022043 5.001075880804747E-4 2.6953850526415196E-4 82-83 0.2741856088910556 5.033550399511271E-4 3.020130239706763E-4 84-85 0.2780695613283559 5.130973955630845E-4 3.052604758413287E-4 86-87 0.28280434615576716 5.260872030456941E-4 3.052604758413287E-4 88-89 0.28825357039472194 5.423244623989564E-4 3.117553795826336E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGATCGG 12155 0.0 28.372633 1 CCGTATC 8135 0.0 28.123707 48-49 TCGTGTA 7805 0.0 27.968655 14-15 ATCGGAA 13925 0.0 26.710047 3 GTAGGGA 8455 0.0 25.762472 18-19 CGCGTGT 4370 0.0 25.326931 3 GAGTGTA 6965 0.0 24.852903 28-29 CGCCGTA 9270 0.0 24.552795 46-47 ACATGCG 5730 0.0 24.206505 4 AAGAGCG 14760 0.0 23.870981 8 CATGCGT 5775 0.0 23.770891 5 TCGGAAG 15060 0.0 23.592855 4 GCCGTAT 7590 0.0 22.912537 48-49 CGTGTAG 7760 0.0 22.835638 16-17 AGAGTGT 8985 0.0 22.492916 26-27 GATCGGA 17240 0.0 21.9325 2 GTCGTGT 8080 0.0 21.842705 14-15 TGTAGGG 8045 0.0 21.790245 18-19 AGAGCGT 16050 0.0 21.690413 9 TTAAAAA 18570 0.0 21.021214 56-57 >>END_MODULE