FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 m48-1.3420000000cb40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 m48-1.3420000000cb40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15396687
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC332370.21587111564974984Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG121550.028.3726331
CCGTATC81350.028.12370748-49
TCGTGTA78050.027.96865514-15
ATCGGAA139250.026.7100473
GTAGGGA84550.025.76247218-19
CGCGTGT43700.025.3269313
GAGTGTA69650.024.85290328-29
CGCCGTA92700.024.55279546-47
ACATGCG57300.024.2065054
AAGAGCG147600.023.8709818
CATGCGT57750.023.7708915
TCGGAAG150600.023.5928554
GCCGTAT75900.022.91253748-49
CGTGTAG77600.022.83563816-17
AGAGTGT89850.022.49291626-27
GATCGGA172400.021.93252
GTCGTGT80800.021.84270514-15
TGTAGGG80450.021.79024518-19
AGAGCGT160500.021.6904139
TTAAAAA185700.021.02121456-57
Produced by FastQC (version 0.11.2)