FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 m12-2.3420000000cc68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 m12-2.3420000000cc68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21890980
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC347010.15851734367305623Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTATC118900.019.6411448-49
AGAGCGT177450.019.2707759
TCGTGTA123250.018.50183314-15
TTAAAAA218950.018.30589756-57
CGCCGTA132350.017.96869946-47
GTAGGGA131950.017.96615218-19
AAGAGCG196950.017.5516018
GCCGTAT103700.016.67814348-49
GAGTGTA103450.016.5718428-29
AGTGTAG125850.016.3391228-29
ATCGGAA227050.015.984063
GTGTAGG151050.015.89901816-17
CATTAAA171150.015.06650354-55
AGAGTGT139550.014.97540226-27
AGATCGG232150.014.5692931
TGTAGGG123400.014.28310718-19
TAGATCT178150.014.20983532-33
CGAATTA80550.013.977941514-15
TGGTCGC163350.013.831058542-43
GTCGTGT124400.013.76719114-15
Produced by FastQC (version 0.11.2)