##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 kx48-1.3420000000cbc6.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16111605 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.475956802565605 33.0 33.0 33.0 33.0 33.0 2 32.45702957588645 33.0 33.0 33.0 33.0 33.0 3 32.448558166613445 33.0 33.0 33.0 33.0 33.0 4 36.321702338159355 37.0 37.0 37.0 37.0 37.0 5 36.31286566422153 37.0 37.0 37.0 37.0 37.0 6 36.33339924855407 37.0 37.0 37.0 37.0 37.0 7 36.36048934913685 37.0 37.0 37.0 37.0 37.0 8 36.368661533099896 37.0 37.0 37.0 37.0 37.0 9 36.3512334742566 37.0 37.0 37.0 37.0 37.0 10-11 36.33586492469248 37.0 37.0 37.0 37.0 37.0 12-13 36.32458619113366 37.0 37.0 37.0 37.0 37.0 14-15 38.660361832356244 40.0 40.0 40.0 37.0 40.0 16-17 38.643149984126346 40.0 40.0 40.0 37.0 40.0 18-19 38.621316001726704 40.0 40.0 40.0 37.0 40.0 20-21 38.589932722407234 40.0 40.0 40.0 37.0 40.0 22-23 38.58582512418843 40.0 40.0 40.0 37.0 40.0 24-25 38.53491775028 40.0 40.0 40.0 37.0 40.0 26-27 38.49816275287286 40.0 40.0 40.0 37.0 40.0 28-29 38.44640568087413 40.0 40.0 40.0 37.0 40.0 30-31 38.397513903797915 40.0 38.5 40.0 37.0 40.0 32-33 38.32610962719109 40.0 37.0 40.0 37.0 40.0 34-35 38.26119353099831 40.0 37.0 40.0 37.0 40.0 36-37 38.19328555410836 40.0 37.0 40.0 37.0 40.0 38-39 38.09900999931416 40.0 37.0 40.0 37.0 40.0 40-41 38.00578061589767 40.0 37.0 40.0 37.0 40.0 42-43 37.90740267651795 40.0 37.0 40.0 35.0 40.0 44-45 37.81701447497006 40.0 37.0 40.0 33.0 40.0 46-47 37.6816493453011 40.0 37.0 40.0 33.0 40.0 48-49 37.55545642411169 40.0 37.0 40.0 33.0 40.0 50-51 36.134105292427414 38.5 35.0 38.5 30.0 40.0 52-53 36.48830054485571 37.0 37.0 40.0 33.0 40.0 54-55 37.177215615700604 38.5 37.0 40.0 33.0 40.0 56-57 37.143780026881245 37.0 37.0 40.0 33.0 40.0 58-59 36.9979326702709 37.0 37.0 40.0 33.0 40.0 60-61 36.84615809536046 37.0 37.0 40.0 33.0 40.0 62-63 36.67237736401805 37.0 37.0 40.0 33.0 40.0 64-65 36.48152331813 37.0 37.0 40.0 33.0 40.0 66-67 36.25571992982698 37.0 37.0 40.0 33.0 40.0 68-69 36.036561534372275 37.0 37.0 37.0 33.0 40.0 70-71 35.83169851793164 37.0 37.0 37.0 33.0 40.0 72-73 35.61414486638668 37.0 37.0 37.0 33.0 40.0 74-75 35.42619121434519 37.0 37.0 37.0 33.0 38.5 76-77 35.23197605080313 37.0 37.0 37.0 33.0 37.0 78-79 35.07391318245451 37.0 37.0 37.0 33.0 37.0 80-81 34.95513618910096 37.0 35.0 37.0 33.0 37.0 82-83 34.85355645821754 37.0 33.0 37.0 33.0 37.0 84-85 34.7565886204385 37.0 33.0 37.0 33.0 37.0 86-87 34.668132038986805 37.0 33.0 37.0 33.0 37.0 88-89 34.58430479148415 37.0 33.0 37.0 33.0 37.0 90-91 34.493572334972214 37.0 33.0 37.0 33.0 37.0 92-93 34.44798193600203 37.0 33.0 37.0 27.0 37.0 94-95 34.39022912366583 37.0 33.0 37.0 27.0 37.0 96-97 34.32628037368096 37.0 33.0 37.0 27.0 37.0 98-99 34.25105946924593 37.0 33.0 37.0 27.0 37.0 100-101 32.57689631169582 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.13265121668225532 2306 2 0.24114503040957658 2306 3 0.2615429363030728 2306 4 0.3088209853281896 2306 5 0.311096140334989 2306 6 0.35801508839695373 2306 7 0.3198108627721439 2306 8 0.2882091893569694 2306 9 0.32859444540638094 2306 10-11 0.3598160426251056 2306 12-13 0.3657760620648176 2306 14-15 0.6198280854048832 2306 16-17 0.5487331660016395 2306 18-19 0.6057797158984357 2306 20-21 0.6430818536110223 2306 22-23 0.6295139069377669 2306 24-25 0.6347494812358221 2306 26-27 0.6678123035091943 2306 28-29 0.6773876972076138 2306 30-31 0.6746387411429495 2306 32-33 0.6384477116077747 2306 34-35 0.6734921661348068 2306 36-37 0.6539692499777487 2306 38-39 0.6814056173639074 2306 40-41 0.6833275731245791 2306 42-43 0.706285886899245 2306 44-45 0.7160507233911346 2306 46-47 0.6795749098419108 2306 48-49 0.7369485909029194 2306 50-51 0.5875240350928976 2306 52-53 0.6132374499874516 2306 54-55 0.7146238143209231 2306 56-57 0.6251532519132255 2306 58-59 0.6676787213329547 2306 60-61 0.6875191631910269 2306 62-63 0.6875818825857891 2306 64-65 0.6939832986523413 2306 66-67 0.7106873836757686 2306 68-69 0.7200715383874225 2306 70-71 0.6951330592559515 2306 72-73 0.7432779560032827 2306 74-75 0.76484096528187 2306 76-77 0.6730786419198793 2306 78-79 0.7377640249676531 2306 80-81 0.7683966978555148 2306 82-83 0.7835741945575023 2306 84-85 0.7996501037995714 2306 86-87 0.7694674275000111 2306 88-89 0.8141628883396308 2306 90-91 0.8558542772935382 2306 92-93 0.8134439432432927 2306 94-95 0.823265510967147 2306 96-97 0.8235582234737961 2306 98-99 0.8639508869291461 2306 100-101 0.7277666514202252 2307 1 -0.030503195582269882 2307 2 0.09513299734764047 2307 3 0.17774924235472866 2307 4 0.23979211510144438 2307 5 0.2577132399009443 2307 6 0.30880734730637727 2307 7 0.3141813528631374 2307 8 0.3090380129860577 2307 9 0.2987333654151243 2307 10-11 0.30491540673345696 2307 12-13 0.3038848300791912 2307 14-15 0.5618401001203068 2307 16-17 0.5590155838808357 2307 18-19 0.5596290064148732 2307 20-21 0.5747873529430478 2307 22-23 0.607831913009953 2307 24-25 0.6114420723543361 2307 26-27 0.6167297517996317 2307 28-29 0.6251134590564291 2307 30-31 0.672529698178856 2307 32-33 0.6556717646588694 2307 34-35 0.6563617537802457 2307 36-37 0.6657815605341995 2307 38-39 0.6653471211439665 2307 40-41 0.6905425332306123 2307 42-43 0.690085603674099 2307 44-45 0.7100670470218802 2307 46-47 0.6816866401448038 2307 48-49 0.7316449617745135 2307 50-51 0.6823637805803955 2307 52-53 0.7003382308678994 2307 54-55 0.710610608091713 2307 56-57 0.6924370431332321 2307 58-59 0.7032418870895967 2307 60-61 0.6974305290907807 2307 62-63 0.7367309316658606 2307 64-65 0.7450608298640446 2307 66-67 0.7466771545043898 2307 68-69 0.7458657007916045 2307 70-71 0.7149721231688204 2307 72-73 0.7643461918562124 2307 74-75 0.7954909363759342 2307 76-77 0.7449140501311149 2307 78-79 0.8350820944999882 2307 80-81 0.7769815189496256 2307 82-83 0.8043131760677866 2307 84-85 0.810303879138246 2307 86-87 0.8072475857788604 2307 88-89 0.8091127677887968 2307 90-91 0.7854459876529276 2307 92-93 0.8356834559302158 2307 94-95 0.759136938833123 2307 96-97 0.8178229732028157 2307 98-99 0.7859671176231302 2307 100-101 0.7836597830666108 2201 1 0.16475371470551892 2201 2 0.23887478520108374 2201 3 0.25915471222302244 2201 4 0.3076438722109103 2201 5 0.3391135115754338 2201 6 0.34125811619033186 2201 7 0.3203512312582788 2201 8 0.30179448542598664 2201 9 0.3072692439263136 2201 10-11 0.3121166934152697 2201 12-13 0.3287914536354961 2201 14-15 0.5316348621003399 2201 16-17 0.5465001436902313 2201 18-19 0.5239268450592007 2201 20-21 0.5758151846946689 2201 22-23 0.5806113268118551 2201 24-25 0.5571219023582898 2201 26-27 0.593073493782974 2201 28-29 0.5686678498019049 2201 30-31 0.6101175398790133 2201 32-33 0.6353181590805761 2201 34-35 0.6059248678057827 2201 36-37 0.6481407012558833 2201 38-39 0.6187086042517507 2201 40-41 0.6227673495144472 2201 42-43 0.6130621223219777 2201 44-45 0.5857740680223174 2201 46-47 0.6333751823215721 2201 48-49 0.6433811416209991 2201 50-51 0.584938499284803 2201 52-53 0.6397301123910211 2201 54-55 0.6148321212566117 2201 56-57 0.6348280381199558 2201 58-59 0.6588867767897071 2201 60-61 0.6985086544781467 2201 62-63 0.6835546975235758 2201 64-65 0.7124086531848235 2201 66-67 0.6910733638570647 2201 68-69 0.703006326328925 2201 70-71 0.7015159209727599 2201 72-73 0.7138860472966613 2201 74-75 0.711097149292705 2201 76-77 0.7385192832105716 2201 78-79 0.724853317547911 2201 80-81 0.7371113391747599 2201 82-83 0.7270463570451753 2201 84-85 0.717654938223582 2201 86-87 0.7357539496565195 2201 88-89 0.678215172562112 2201 90-91 0.7091562291343578 2201 92-93 0.6835348891522202 2201 94-95 0.7371481458723892 2201 96-97 0.7292935453391323 2201 98-99 0.7437491869467721 2201 100-101 0.6893129654408412 1101 1 0.0027066313033330403 1101 2 0.14926416625665695 1101 3 0.19001079761621753 1101 4 0.2810610245291798 1101 5 0.3116388469863338 1101 6 0.3165261246109168 1101 7 0.3059977835308558 1101 8 0.33163404700722765 1101 9 0.341336160698134 1101 10-11 0.30418581491631613 1101 12-13 0.33678087583346183 1101 14-15 0.5355139705386449 1101 16-17 0.5507771893162925 1101 18-19 0.589270172228531 1101 20-21 0.5757533957183725 1101 22-23 0.6141355743837451 1101 24-25 0.5986337794112231 1101 26-27 0.6088283588857166 1101 28-29 0.6253273573684908 1101 30-31 0.6130591217253496 1101 32-33 0.6184946876024995 1101 34-35 0.6392121182016695 1101 36-37 0.6140993513852777 1101 38-39 0.6950157651684492 1101 40-41 0.6825681751718307 1101 42-43 0.6747819457112243 1101 44-45 0.6545877641735984 1101 46-47 0.643277837977017 1101 48-49 0.6367102121493176 1101 50-51 0.5630063637879132 1101 52-53 0.6201977904956948 1101 54-55 0.6878812260344631 1101 56-57 0.6675943433675329 1101 58-59 0.6768110683870461 1101 60-61 0.6639533740387478 1101 62-63 0.6577027267374689 1101 64-65 0.6371184867831801 1101 66-67 0.7052754695032917 1101 68-69 0.6509090902460812 1101 70-71 0.6555584829038636 1101 72-73 0.7271684707792829 1101 74-75 0.7105838254896355 1101 76-77 0.7165854101572862 1101 78-79 0.7457264354066027 1101 80-81 0.7212834599298148 1101 82-83 0.752231161336745 1101 84-85 0.7854195088566627 1101 86-87 0.7488139659207818 1101 88-89 0.7008281635560181 1101 90-91 0.7861659787931146 1101 92-93 0.800385106127969 1101 94-95 0.8235040275026151 1101 96-97 0.7798004760378845 1101 98-99 0.762561406634795 1101 100-101 0.7038396355892402 2304 1 -0.013862830931067549 2304 2 0.13419137339099052 2304 3 0.16722948286122374 2304 4 0.21023987826986001 2304 5 0.23162356914703253 2304 6 0.28555605831164854 2304 7 0.26466824580530357 2304 8 0.30001050119591355 2304 9 0.25955940793132015 2304 10-11 0.32527954260975633 2304 12-13 0.31508365837289176 2304 14-15 0.5202515594923298 2304 16-17 0.53530043249242 2304 18-19 0.5379461508860857 2304 20-21 0.5248725725421082 2304 22-23 0.5259588531886266 2304 24-25 0.5763268050326147 2304 26-27 0.5064243479976085 2304 28-29 0.5654510989453243 2304 30-31 0.5396263523010347 2304 32-33 0.5292983470361534 2304 34-35 0.5568586493050702 2304 36-37 0.5563612789577661 2304 38-39 0.6224431345574715 2304 40-41 0.6005681275046086 2304 42-43 0.6474734991972966 2304 44-45 0.6183301579491314 2304 46-47 0.6208455943113762 2304 48-49 0.670999112728289 2304 50-51 0.59537331351045 2304 52-53 0.5723574295019063 2304 54-55 0.6564546346569173 2304 56-57 0.652583281938341 2304 58-59 0.6807257428232063 2304 60-61 0.672237349411283 2304 62-63 0.684044022836261 2304 64-65 0.688554533448162 2304 66-67 0.7006573350265626 2304 68-69 0.7361174307106069 2304 70-71 0.7314989329520287 2304 72-73 0.7283982148175951 2304 74-75 0.7706127996342715 2304 76-77 0.7896386114299574 2304 78-79 0.8119319448331623 2304 80-81 0.7637436469780923 2304 82-83 0.7579186745196509 2304 84-85 0.7789309339741521 2304 86-87 0.7390700024451462 2304 88-89 0.7701274151641897 2304 90-91 0.7426909096023806 2304 92-93 0.7713402256409623 2304 94-95 0.7751097148830794 2304 96-97 0.7806300348413302 2304 98-99 0.7628213551516296 2304 100-101 0.8722701273650131 2202 1 0.11288756197924243 2202 2 0.12464260307054786 2202 3 0.14643096706360126 2202 4 0.2222053666103747 2202 5 0.21579026459197337 2202 6 0.2526419473621928 2202 7 0.2022788180716617 2202 8 0.22511583993571804 2202 9 0.18921880767781118 2202 10-11 0.22690762977872936 2202 12-13 0.23952573114832632 2202 14-15 0.37314245886990705 2202 16-17 0.4207377323113519 2202 18-19 0.41080626191870095 2202 20-21 0.40161115171849104 2202 22-23 0.42170392254825373 2202 24-25 0.4439614030920538 2202 26-27 0.4303110096972773 2202 28-29 0.4546135853182065 2202 30-31 0.48053148252467537 2202 32-33 0.47681368081941855 2202 34-35 0.4637820206783232 2202 36-37 0.4489886501539786 2202 38-39 0.5008285479698174 2202 40-41 0.5041556195363981 2202 42-43 0.46763981642759234 2202 44-45 0.45713747172574415 2202 46-47 0.4786041981786653 2202 48-49 0.4824748679493851 2202 50-51 0.4150354896007258 2202 52-53 0.4741528411817484 2202 54-55 0.49667665162424157 2202 56-57 0.49497592232886944 2202 58-59 0.5025464836521891 2202 60-61 0.5205150547959008 2202 62-63 0.494940293402621 2202 64-65 0.47949013141887065 2202 66-67 0.5218250376042946 2202 68-69 0.48379342853842644 2202 70-71 0.5369641592173195 2202 72-73 0.546309232355938 2202 74-75 0.5241218152470708 2202 76-77 0.5353233662930776 2202 78-79 0.5806545865558377 2202 80-81 0.5361493984922063 2202 82-83 0.5531922597150611 2202 84-85 0.583527926744047 2202 86-87 0.568374681453939 2202 88-89 0.5984467852469706 2202 90-91 0.5897140875818181 2202 92-93 0.5836695556388349 2202 94-95 0.6186436005610929 2202 96-97 0.6345591853131438 2202 98-99 0.6456662868566951 2202 100-101 0.6702728233102775 1102 1 0.04743052852093399 1102 2 0.10523101596054829 1102 3 0.1008677710004875 1102 4 0.14852035979875922 1102 5 0.16313920509350055 1102 6 0.1877563197039933 1102 7 0.16085595008734543 1102 8 0.17647745402877746 1102 9 0.17843774457934813 1102 10-11 0.16806228825569036 1102 12-13 0.20044184458927106 1102 14-15 0.2812136671094976 1102 16-17 0.30503447428515074 1102 18-19 0.3256168165808333 1102 20-21 0.30428507420317175 1102 22-23 0.3385302698940862 1102 24-25 0.3130155914087638 1102 26-27 0.3413557569852941 1102 28-29 0.3412222918945744 1102 30-31 0.3616388885787458 1102 32-33 0.37789090908253087 1102 34-35 0.37518170147456686 1102 36-37 0.3306343664003464 1102 38-39 0.3687672792192984 1102 40-41 0.3806502408247141 1102 42-43 0.41139484408943616 1102 44-45 0.4099124033563015 1102 46-47 0.4146580805298541 1102 48-49 0.4068622108381206 1102 50-51 0.32654222455691695 1102 52-53 0.3783603060649483 1102 54-55 0.43672801060418465 1102 56-57 0.4528502792440534 1102 58-59 0.45167178024623666 1102 60-61 0.49310446190497004 1102 62-63 0.5271622447061475 1102 64-65 0.5110359867343277 1102 66-67 0.5179903373323 1102 68-69 0.5075682325144939 1102 70-71 0.5091976196873134 1102 72-73 0.5325894143682746 1102 74-75 0.5362118434053542 1102 76-77 0.5342312112354222 1102 78-79 0.5482313599231858 1102 80-81 0.508102559426689 1102 82-83 0.5149043562947995 1102 84-85 0.5174771986069047 1102 86-87 0.5170764893521707 1102 88-89 0.5846452413706373 1102 90-91 0.5722237342760721 1102 92-93 0.5502024665191172 1102 94-95 0.6306090136246709 1102 96-97 0.6110525233023694 1102 98-99 0.5680439158468289 1102 100-101 0.5623267705018407 2305 1 0.11564522271398658 2305 2 0.11781965417315376 2305 3 0.13746278976047677 2305 4 0.1463305708250786 2305 5 0.1788471384804069 2305 6 0.17169890537998356 2305 7 0.18855699044318897 2305 8 0.23682017294487423 2305 9 0.1943736777215932 2305 10-11 0.2202946064662541 2305 12-13 0.20946861117122495 2305 14-15 0.3201337612066766 2305 16-17 0.33921373864770743 2305 18-19 0.3997255326212681 2305 20-21 0.3750370039754145 2305 22-23 0.3634654476079575 2305 24-25 0.3529816787257758 2305 26-27 0.40911092416763495 2305 28-29 0.4112724347002299 2305 30-31 0.42273896197459493 2305 32-33 0.39845872809149796 2305 34-35 0.40000918330895274 2305 36-37 0.4347130364456291 2305 38-39 0.41069906317330407 2305 40-41 0.4745352552261437 2305 42-43 0.4236544462869247 2305 44-45 0.4458796242974046 2305 46-47 0.46996802435412377 2305 48-49 0.46568395213777336 2305 50-51 0.33706473509190715 2305 52-53 0.42324261077204994 2305 54-55 0.46994048373124286 2305 56-57 0.46088683879343506 2305 58-59 0.49254929891550603 2305 60-61 0.49644609781450555 2305 62-63 0.5113835625237897 2305 64-65 0.49205183411993403 2305 66-67 0.5418159274282814 2305 68-69 0.5341162861786799 2305 70-71 0.5406937045068432 2305 72-73 0.5419253772625581 2305 74-75 0.5062014103987877 2305 76-77 0.5690518135391116 2305 78-79 0.5485214520727979 2305 80-81 0.5746593683592351 2305 82-83 0.5100515808935526 2305 84-85 0.5385654420443302 2305 86-87 0.5401240657900033 2305 88-89 0.5143493089002362 2305 90-91 0.5329268978732884 2305 92-93 0.5928352820201539 2305 94-95 0.5850791133636406 2305 96-97 0.5614990023179374 2305 98-99 0.574902319097184 2305 100-101 0.6614452453700395 2203 1 0.10713391223336544 2203 2 0.099683307042433 2203 3 0.13717570720417882 2203 4 0.11762229135779734 2203 5 0.14930364185778444 2203 6 0.14867883343850963 2203 7 0.14866885172820332 2203 8 0.12320977408843703 2203 9 0.15097499529782965 2203 10-11 0.1728569871299399 2203 12-13 0.17437434988239886 2203 14-15 0.2491586260681018 2203 16-17 0.22951841325436817 2203 18-19 0.2564767878625318 2203 20-21 0.2874753900835785 2203 22-23 0.29231839268722837 2203 24-25 0.2658057113417911 2203 26-27 0.29742904390005265 2203 28-29 0.3151856289763728 2203 30-31 0.29742268646751313 2203 32-33 0.31146452139046943 2203 34-35 0.33458151532037306 2203 36-37 0.30271778884188905 2203 38-39 0.3487659399804812 2203 40-41 0.3543320447961378 2203 42-43 0.36892089751586354 2203 44-45 0.36288821293663176 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-0.9799048441230482 1213 74-75 -0.9925273609724599 1213 76-77 -0.9844591311044368 1213 78-79 -0.9643932851192929 1213 80-81 -1.035119394693183 1213 82-83 -1.049898760980433 1213 84-85 -1.0817016062186937 1213 86-87 -1.0406920686226826 1213 88-89 -1.0264420555962985 1213 90-91 -1.0388523543590722 1213 92-93 -1.0131919467884387 1213 94-95 -1.1721520862704224 1213 96-97 -1.2162000038184786 1213 98-99 -1.0738703182949934 1213 100-101 -1.051809574869985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14261.0 3 1551.0 4 981.0 5 775.0 6 613.0 7 754.0 8 1334.0 9 4010.0 10 13972.0 11 21601.0 12 17647.0 13 16847.0 14 17097.0 15 18498.0 16 20508.0 17 23305.0 18 26312.0 19 29835.0 20 33045.0 21 37106.0 22 41553.0 23 46588.0 24 53263.0 25 60343.0 26 70550.0 27 85713.0 28 99009.0 29 118626.0 30 146471.0 31 188205.0 32 250833.0 33 342860.0 34 491349.0 35 790543.0 36 1814964.0 37 5553751.0 38 5650500.0 39 6432.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 21.70679818229456 12.898398520822152 15.729057334925065 49.66574596195822 2 22.858705630877992 18.384428486985513 36.82306302408971 21.933802858046782 3 25.451402244914657 22.75618559943667 25.48143232347896 26.310979832169718 4 27.422126191953332 27.426756977552746 18.884374753503906 26.266742076990013 5 28.78119963883713 29.410816548221614 22.041912919964503 19.76607089297675 6 21.744109877501955 33.082543262700675 22.86688384975361 22.30646301004376 7 19.403185480395965 16.04087133679665 41.86986340991014 22.68607977289725 8 21.543224400901238 21.09963163689054 27.946219868295913 29.410924093912307 9 22.136421824599413 20.879608455467352 30.764142279933566 26.219827439999673 10-11 24.820795571444393 29.148396404624872 21.523244009659606 24.507564014271132 12-13 22.965708422131197 23.397824198185273 28.247752963176342 25.38871441650719 14-15 23.536559865630398 25.200328658731237 26.695976969650527 24.567134505987838 16-17 24.575309365274503 25.145947539127928 25.73830264464866 24.540440450948918 18-19 24.40729463070623 25.53874905116553 25.738161211932738 24.315795106195505 20-21 24.29031006091729 25.54079022697408 25.89069676749631 24.27820294461232 22-23 24.246215366966975 25.395198741171864 25.725224062184598 24.633361829676563 24-25 24.210970155728806 25.652460412702837 25.55895389046054 24.577615541107814 26-27 24.105511655704184 25.71118473914676 25.55359459954455 24.62970900560451 28-29 24.334997301157316 25.51143853457643 25.337607026567667 24.815957137698586 30-31 24.45392420173546 25.275819883645102 25.532186454232026 24.738069460387415 32-33 24.402904448122733 25.48551146542875 25.432858588644436 24.678725497804074 34-35 24.17274693559976 25.67683014090863 25.250520005217297 24.899902918274314 36-37 24.01778973089667 25.490932297597986 25.607967384291914 24.883310587213433 38-39 24.18135466033442 25.4218789659587 25.723907427072657 24.672858946634225 40-41 24.714971365427424 25.190970451746892 25.400508392536132 24.693549790289556 42-43 24.384947745508384 25.33495793683021 25.783919924520514 24.49617439314089 44-45 24.504731778076607 25.24661919637035 25.746197989336505 24.502451036216534 46-47 24.54604332048182 25.208656263361952 25.525625542948287 24.719674873207936 48-49 24.175617340296885 25.494306879101735 25.386035108594484 24.944040672006892 50-51 24.014429251585145 25.498249681155517 25.98802626746434 24.499294799795006 52-53 24.316971381999515 25.508501109491423 25.677641621315754 24.496885887193308 54-55 24.111685779730653 25.649615156496587 25.538288019526767 24.700411044246 56-57 23.803983170120294 25.413174901374852 26.119618630678204 24.663223297826654 58-59 24.311307854183745 25.426915017773204 25.6243612620367 24.63741586600635 60-61 24.203769035868135 25.355958112157634 25.776045137707605 24.66422771426663 62-63 24.08140814354798 25.55804259965249 25.83501756569811 24.525531691101417 64-65 24.214065277307853 25.638488948790577 25.434810773729133 24.712635000172433 66-67 24.167391128780285 25.649336555116854 25.362036585976345 24.821235730126524 68-69 24.148905040446756 25.592396620799075 25.60198592423251 24.65671241452166 70-71 24.391647498683884 25.523522681684884 25.339530553027167 24.74529926660406 72-73 24.275828939001272 25.682603509882917 25.53834833809808 24.503219213017736 74-75 24.603371838658415 25.450722120551582 25.48300908818924 24.46289695260076 76-77 24.837187479449536 25.354529776658158 25.36641970095783 24.44186304293447 78-79 24.39953189967792 25.47310575772908 25.439866743040035 24.687495599552964 80-81 24.51659199101717 25.56620186049674 25.285416901941215 24.631789246544873 82-83 24.533700871128083 25.52899041045397 25.206823125636635 24.73048559278131 84-85 24.45178106772303 25.450854358712732 25.19584857482172 24.901515998742514 86-87 24.344809819689424 25.776662984580383 25.371961816627504 24.50656537910269 88-89 24.495053015226954 25.62699534393208 25.298542836710432 24.579408804130537 90-91 24.570146862649278 25.49214875679909 25.356046556617446 24.58165782393419 92-93 24.48436529450101 25.702489783489064 25.616698492651835 24.196446429358087 94-95 24.44784989690696 25.589182197937703 25.360272619846334 24.602695285308997 96-97 24.23300484511526 25.871563372683404 25.27404534626226 24.62138643593908 98-99 24.47267222726467 25.45666735030515 25.5114930649013 24.559167357528874 100-101 24.587392237418847 25.623397431068 25.33837926594052 24.450831065572633 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12527.0 1 6671.5 2 789.5 3 713.0 4 603.0 5 525.0 6 477.5 7 440.5 8 462.0 9 452.0 10 445.5 11 514.5 12 561.5 13 626.5 14 726.5 15 858.5 16 1043.0 17 1306.0 18 1626.5 19 1940.0 20 2478.5 21 3372.5 22 4736.5 23 6741.0 24 9476.5 25 13402.0 26 18693.5 27 25701.0 28 35130.5 29 46958.5 30 61464.0 31 78667.5 32 99407.5 33 125307.5 34 158199.0 35 203088.5 36 257185.5 37 326541.0 38 399346.5 39 462214.0 40 524661.5 41 581636.0 42 629538.0 43 668208.0 44 705877.5 45 707299.5 46 679185.5 47 669238.5 48 634122.5 49 588335.0 50 578478.5 51 548522.0 52 503940.5 53 476350.5 54 452408.0 55 439302.5 56 443485.0 57 440871.5 58 409085.5 59 352881.5 60 313405.0 61 293149.0 62 262038.0 63 233060.5 64 203206.5 65 185028.0 66 180562.0 67 175135.5 68 166881.0 69 144216.0 70 116176.0 71 93096.0 72 73068.5 73 62336.5 74 57407.5 75 47047.5 76 30865.5 77 18864.0 78 13700.0 79 14511.0 80 13074.5 81 8281.5 82 3732.0 83 1009.5 84 685.0 85 489.5 86 387.0 87 332.5 88 263.0 89 208.0 90 182.5 91 161.5 92 146.0 93 112.0 94 73.0 95 47.0 96 32.0 97 18.5 98 13.0 99 10.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1507608956401302 2 0.14272321100225582 3 0.1514498400376623 4 0.14661481584236952 5 0.15318151109091863 6 0.10387543637024368 7 0.10794703569259548 8 0.10481885572542277 9 0.09274681200290101 10-11 0.07952032091154171 12-13 0.08802971522700563 14-15 0.13256593616837056 16-17 0.14136394232604385 18-19 0.12916776447784067 20-21 0.16152332433671257 22-23 0.14269838417711955 24-25 0.13873850556788103 26-27 0.12918638459669288 28-29 0.12691783344986424 30-31 0.09144030033010367 32-33 0.10283270971451944 34-35 0.11779087185913507 36-37 0.12320001638570457 38-39 0.12151799898272084 40-41 0.15528868787436137 42-43 0.13614720569428063 44-45 0.12630336952774102 46-47 0.16025094954847763 48-49 0.1824088909826178 50-51 0.1576068926714626 52-53 0.10733877847675635 54-55 0.10921010042140432 56-57 0.11151278845279536 58-59 0.11083625746783142 60-61 0.11563093807227771 62-63 0.09773079714901153 64-65 0.11525853569523334 66-67 0.12991567258507145 68-69 0.12884811910421093 70-71 0.11666745802171789 72-73 0.12616682198949142 74-75 0.16241398668847704 76-77 0.1909338020637919 78-79 0.16504563015292392 80-81 0.13807438799548524 82-83 0.12198970866031038 84-85 0.1290591471178694 86-87 0.12246762504418399 88-89 0.12560821842392486 90-91 0.14022501172291651 92-93 0.10746291260243782 94-95 0.11254310169595146 96-97 0.11347721099170442 98-99 0.1166333211371555 100-101 0.10997352529434527 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.6111605E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.01489350553744 #Duplication Level Percentage of deduplicated Percentage of total 1 75.83484310542988 37.928716019272166 2 14.077698247184427 14.081891572720407 3 4.362785698765107 6.546127863336556 4 1.9805163243225774 3.9622125218788864 5 0.9671735121904536 2.418654010679108 6 0.6505369635556875 1.9521922172192039 7 0.455094734502669 1.5933060276757278 8 0.299482050874308 1.198285030503877 9 0.18639269261663366 0.8390169604288176 >10 1.0169081771205697 8.959804258734296 >50 0.08321063123403605 2.9244046284240115 >100 0.06821569123291703 7.041662981554292 >500 0.010547670089416653 3.6155443519928223 >1k 0.006275634815623456 5.678748833033716 >5k 2.5667462801078826E-4 0.8894927136455667 >10k+ 6.219143788232978E-5 0.36994000890054524 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.03219418549548602 1.2413412568145755E-5 6.206706284072878E-6 2 0.032256252558326745 1.2413412568145755E-5 6.206706284072878E-6 3 0.03233693974001969 1.2413412568145755E-5 6.206706284072878E-6 4 0.0326100348165189 1.2413412568145755E-5 1.2413412568145755E-5 5 0.03269692870449592 1.8620118852218633E-5 1.2413412568145755E-5 6 0.032914163424438474 3.7240237704437266E-5 1.8620118852218633E-5 7 0.033007264018699564 3.7240237704437266E-5 1.8620118852218633E-5 8 0.03311898473181288 3.7240237704437266E-5 3.103353142036439E-5 9 0.03323691215121026 3.7240237704437266E-5 3.7240237704437266E-5 10-11 0.033500697168283355 4.344694398851014E-5 4.3446943988510147E-5 12-13 0.03379241236363478 4.965365027258302E-5 4.655029713054659E-5 14-15 0.03415240132811101 7.13771222668381E-5 5.896370969869234E-5 16-17 0.034382049460621705 9.620394740312961E-5 6.517041598276522E-5 18-19 0.03458997412113815 1.0241065368720248E-4 8.068718169294741E-5 20-21 0.03505858044558565 1.1482406625534824E-4 8.999724111905674E-5 22-23 0.035288228578096346 1.1792741939738468E-4 1.0241065368720249E-4 24-25 0.03547753311976057 1.3965089139163976E-4 1.1792741939738468E-4 26-27 0.0356668376614248 1.4585759767571263E-4 1.334441851075669E-4 28-29 0.03585303884994698 1.520643039597855E-4 1.427542445336762E-4 30-31 0.036076480276173606 1.5516765710182196E-4 1.4585759767571263E-4 32-33 0.036275094877263936 1.582710102438584E-4 1.520643039597855E-4 34-35 0.03643336588750779 1.6447771652793126E-4 1.8930454166422278E-4 36-37 0.036650600607450345 1.7378777595404059E-4 1.9861460109033208E-4 38-39 0.0369019722119553 1.7689112909607702E-4 1.9861460109033208E-4 40-41 0.03717196393531247 1.7999448223811345E-4 2.079246605164414E-4 42-43 0.03739230200839705 1.9240789480625922E-4 2.668883702151338E-4 44-45 0.0376095367283396 2.1723471994255074E-4 2.793017827832795E-4 46-47 0.03782366809514012 2.234414262266236E-4 2.8240513592531593E-4 48-49 0.0381122799373495 2.3275148565273294E-4 2.855084890673524E-4 50-51 0.038503302433246095 2.3585483879476937E-4 2.979219016354981E-4 52-53 0.03892535846056305 2.389581919368058E-4 3.04128607919571E-4 54-55 0.03936603460673223 2.4206154507884224E-4 3.1964537362975324E-4 56-57 0.03988429458145232 2.4206154507884224E-4 3.2274872677178965E-4 58-59 0.04031255731505334 2.4826825136291513E-4 3.2585207991382605E-4 60-61 0.0409052977651823 2.793017827832795E-4 3.289554330558625E-4 62-63 0.041631482400418834 3.2274872677178965E-4 3.32058786197899E-4 64-65 0.04238559721393369 3.3826549248197184E-4 3.3826549248197184E-4 66-67 0.04312109190859632 3.444721987660447E-4 3.5378225819215404E-4 68-69 0.04394658384437801 3.599889644762269E-4 3.5378225819215404E-4 70-71 0.04504206750351687 3.81712436470482E-4 3.5688561133419045E-4 72-73 0.0462709953477633 4.0964261474880995E-4 3.6309231761826337E-4 74-75 0.04762405731769119 4.4067614616917435E-4 3.8481578961251843E-4 76-77 0.049076426588164246 4.5619291187935654E-4 4.065392616067735E-4 78-79 0.05064361992489265 4.6550297130546587E-4 4.1895267417491923E-4 80-81 0.05260493911065968 4.779163838736116E-4 4.2515938045899215E-4 82-83 0.05527071945966898 4.8412309015768447E-4 4.5619291187935654E-4 84-85 0.05866578779705684 4.903297964417573E-4 4.686063244475023E-4 86-87 0.06246118868976741 5.08949915293976E-4 4.779163838736116E-4 88-89 0.06711001169653799 5.213633278621217E-4 4.903297964417573E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCGTAT 3395 0.0 39.59659 1 GCGTATT 4580 0.0 29.558592 2 TAAGTCG 4815 0.0 27.224869 9 ACATGCG 5175 0.0 25.608532 4 CATGCGT 5400 0.0 24.541052 5 CGCGTGT 1925 0.0 21.71415 3 TTAAGTC 6795 0.0 19.775793 8 GAGCGTC 5885 0.0 19.611591 9 GTATTTA 8475 0.0 17.094286 4 GCCAGTA 8245 0.0 17.05265 3 CCGTATC 4605 0.0 17.024595 48-49 CGTATTT 8845 0.0 16.808825 3 GTCCGTA 4070 0.0 16.45567 4 TCCGTAC 4160 0.0 16.327717 5 TCGTGTA 4395 0.0 16.268396 14-15 TACCCTA 5735 0.0 16.232138 9 GTTAGTT 5915 0.0 16.222124 1 CGCCGTA 4880 0.0 15.676124 46-47 AGTTACG 3345 0.0 15.330124 6 ATGCGTG 9900 0.0 14.963647 6 >>END_MODULE