FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 kx48-1.3420000000cbc6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 kx48-1.3420000000cbc6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16111605
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT33950.039.596591
GCGTATT45800.029.5585922
TAAGTCG48150.027.2248699
ACATGCG51750.025.6085324
CATGCGT54000.024.5410525
CGCGTGT19250.021.714153
TTAAGTC67950.019.7757938
GAGCGTC58850.019.6115919
GTATTTA84750.017.0942864
GCCAGTA82450.017.052653
CCGTATC46050.017.02459548-49
CGTATTT88450.016.8088253
GTCCGTA40700.016.455674
TCCGTAC41600.016.3277175
TCGTGTA43950.016.26839614-15
TACCCTA57350.016.2321389
GTTAGTT59150.016.2221241
CGCCGTA48800.015.67612446-47
AGTTACG33450.015.3301246
ATGCGTG99000.014.9636476
Produced by FastQC (version 0.11.2)