FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 kx24-1.3420000000c9e2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 kx24-1.3420000000c9e2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15056799
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA53850.012.83636114-15
CTCGGAT79450.012.19735051
CTCGGGT86700.011.780091
CTCGGTT65150.011.5205151
CGCGTAT11300.011.3504891
CTCGACT76900.011.3045691
CTCGTTT67950.011.2555231
CTCGATT84150.011.1773881
CTCGTAT40750.010.9579541
CGCAAAT35000.010.8580341
CTGGGAT105750.010.2419761
TTAGGTG70100.09.96268118-19
CTGGAAT141850.09.9126921
CGGGAAT44350.09.8542471
TTACTCG42950.09.8436163
GAGTGTC73200.09.66888224-25
AATTAGG74650.09.61004716-17
CTCAGAT121900.09.5865131
CTCAAAT122450.09.5434541
CGACAAT55800.09.5348321
Produced by FastQC (version 0.11.2)