##FastQC 0.11.2 >>Basic Statistics pass #Measure Value Filename C5B16ACXX l06n02 kx24-1.3420000000c9e2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15056799 Sequences flagged as poor quality 0 Sequence length 101 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.456211575913315 33.0 33.0 33.0 33.0 33.0 2 32.44296400582886 33.0 33.0 33.0 33.0 33.0 3 32.43483339320662 33.0 33.0 33.0 33.0 33.0 4 36.303559807101095 37.0 37.0 37.0 37.0 37.0 5 36.29105867721287 37.0 37.0 37.0 37.0 37.0 6 36.291495091353745 37.0 37.0 37.0 37.0 37.0 7 36.33804927594504 37.0 37.0 37.0 37.0 37.0 8 36.33522942027718 37.0 37.0 37.0 37.0 37.0 9 36.33332436728418 37.0 37.0 37.0 37.0 37.0 10-11 36.31337779696734 37.0 37.0 37.0 37.0 37.0 12-13 36.2973120315945 37.0 37.0 37.0 37.0 37.0 14-15 38.60904070646092 40.0 40.0 40.0 37.0 40.0 16-17 38.590174345822106 40.0 40.0 40.0 37.0 40.0 18-19 38.564792490090355 40.0 40.0 40.0 37.0 40.0 20-21 38.52864430215214 40.0 40.0 40.0 37.0 40.0 22-23 38.49592977896563 40.0 40.0 40.0 37.0 40.0 24-25 38.46001490755107 40.0 40.0 40.0 37.0 40.0 26-27 38.41991803835596 40.0 40.0 40.0 37.0 40.0 28-29 38.37382401133202 40.0 37.0 40.0 37.0 40.0 30-31 38.32566832432312 40.0 37.0 40.0 37.0 40.0 32-33 38.25524681574085 40.0 37.0 40.0 37.0 40.0 34-35 38.18255105218579 40.0 37.0 40.0 37.0 40.0 36-37 38.10417353648674 40.0 37.0 40.0 37.0 40.0 38-39 37.99982755298785 40.0 37.0 40.0 37.0 40.0 40-41 37.89546396946655 40.0 37.0 40.0 33.0 40.0 42-43 37.801135951937724 40.0 37.0 40.0 33.0 40.0 44-45 37.697366717852844 40.0 37.0 40.0 33.0 40.0 46-47 37.56417472930335 40.0 37.0 40.0 33.0 40.0 48-49 37.425008363331415 40.0 37.0 40.0 33.0 40.0 50-51 36.00634763072815 38.5 35.0 38.5 30.0 40.0 52-53 36.35296473041846 37.0 37.0 40.0 33.0 40.0 54-55 37.03744786657509 37.0 37.0 40.0 33.0 40.0 56-57 36.99851625169467 37.0 37.0 40.0 33.0 40.0 58-59 36.85772742931616 37.0 37.0 40.0 33.0 40.0 60-61 36.68463240427132 37.0 37.0 40.0 33.0 40.0 62-63 36.50350276974542 37.0 37.0 40.0 33.0 40.0 64-65 36.3114122729539 37.0 37.0 40.0 33.0 40.0 66-67 36.08766740526987 37.0 37.0 40.0 33.0 40.0 68-69 35.87405998446283 37.0 37.0 37.0 33.0 40.0 70-71 35.66955934657825 37.0 37.0 37.0 33.0 40.0 72-73 35.447745267769065 37.0 37.0 37.0 33.0 40.0 74-75 35.24582070863801 37.0 37.0 37.0 33.0 38.5 76-77 35.050363028688906 37.0 37.0 37.0 33.0 37.0 78-79 34.892932388882926 37.0 33.0 37.0 33.0 37.0 80-81 34.766695663533795 37.0 33.0 37.0 33.0 37.0 82-83 34.664856786625094 37.0 33.0 37.0 33.0 37.0 84-85 34.561749545836406 37.0 33.0 37.0 30.0 37.0 86-87 34.47148470933297 37.0 33.0 37.0 27.0 37.0 88-89 34.38678802180995 37.0 33.0 37.0 27.0 37.0 90-91 34.28745000846462 37.0 33.0 37.0 27.0 37.0 92-93 34.228580822524094 37.0 33.0 37.0 27.0 37.0 94-95 34.15828507108317 37.0 33.0 37.0 27.0 37.0 96-97 34.089218066868 37.0 33.0 37.0 27.0 37.0 98-99 34.01008142567355 37.0 33.0 37.0 27.0 37.0 100-101 32.29710166151517 35.0 30.0 37.0 24.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 2306 1 0.1471375195883482 2306 2 0.21954867683827217 2306 3 0.258102742779883 2306 4 0.33122678490229873 2306 5 0.35119011091511965 2306 6 0.3859633825173958 2306 7 0.3697030973326818 2306 8 0.35814850498982054 2306 9 0.3747089379870374 2306 10-11 0.3915165904402045 2306 12-13 0.39746317308340195 2306 14-15 0.6356049457485113 2306 16-17 0.5494107958346817 2306 18-19 0.6511865161858594 2306 20-21 0.6759667633127293 2306 22-23 0.6512786203539775 2306 24-25 0.6666027643506709 2306 26-27 0.6998554946824882 2306 28-29 0.742875432586672 2306 30-31 0.7650216724536065 2306 32-33 0.7116821184572402 2306 34-35 0.7355713573316933 2306 36-37 0.744130266433153 2306 38-39 0.74109681789664 2306 40-41 0.7414566543864538 2306 42-43 0.7104110479337038 2306 44-45 0.7624087253881981 2306 46-47 0.725685938670324 2306 48-49 0.7656201369289093 2306 50-51 0.6507653742458146 2306 52-53 0.677026855317429 2306 54-55 0.7540185262843906 2306 56-57 0.6964545127377022 2306 58-59 0.7333387138723211 2306 60-61 0.7583411900068668 2306 62-63 0.7431188281457395 2306 64-65 0.736621902403499 2306 66-67 0.7594211405739415 2306 68-69 0.7897791011013808 2306 70-71 0.7502175851875279 2306 72-73 0.8148513152320191 2306 74-75 0.7907636517850278 2306 76-77 0.6776085232541575 2306 78-79 0.7440463700824935 2306 80-81 0.7648670696925635 2306 82-83 0.7887872829249432 2306 84-85 0.8234652371459674 2306 86-87 0.8176660355340957 2306 88-89 0.813532949550428 2306 90-91 0.8667246134012245 2306 92-93 0.8947991801517148 2306 94-95 0.8977544388630889 2306 96-97 0.8651061880429936 2306 98-99 0.896619487163484 2306 100-101 0.7913348557545632 2307 1 -0.03785807449951761 2307 2 0.14478018144062332 2307 3 0.25428963114050873 2307 4 0.31422812110336196 2307 5 0.31601939014340985 2307 6 0.39157231952728466 2307 7 0.34215832238933785 2307 8 0.36512050025762477 2307 9 0.36477338416276694 2307 10-11 0.3711360283923071 2307 12-13 0.3706340814115734 2307 14-15 0.621104084542516 2307 16-17 0.6012245445001412 2307 18-19 0.6378572724712157 2307 20-21 0.6528252092354236 2307 22-23 0.6922124573152502 2307 24-25 0.6583378780276448 2307 26-27 0.7168431991911746 2307 28-29 0.7190379497053598 2307 30-31 0.7253728747016268 2307 32-33 0.7082682579237769 2307 34-35 0.7549969074048306 2307 36-37 0.7454350878549789 2307 38-39 0.7470754298012565 2307 40-41 0.7836542501123844 2307 42-43 0.7498029821180339 2307 44-45 0.7890938203095175 2307 46-47 0.7766356513427084 2307 48-49 0.7878822842617197 2307 50-51 0.7470706506862825 2307 52-53 0.7923020250615735 2307 54-55 0.7772739460002356 2307 56-57 0.8048164789508974 2307 58-59 0.7456138958242846 2307 60-61 0.776501649553488 2307 62-63 0.7405009172805563 2307 64-65 0.7602341233161525 2307 66-67 0.8050187520590555 2307 68-69 0.7758162994295787 2307 70-71 0.726458787235778 2307 72-73 0.7902023454607914 2307 74-75 0.8183840319586508 2307 76-77 0.8182628566962507 2307 78-79 0.8781982930356307 2307 80-81 0.8477856979334604 2307 82-83 0.8507981656388921 2307 84-85 0.8243245287374421 2307 86-87 0.8237795160715038 2307 88-89 0.8231732129285305 2307 90-91 0.8621759530426729 2307 92-93 0.9020533979524927 2307 94-95 0.8861210828898081 2307 96-97 0.9561249525675137 2307 98-99 0.8920947430282311 2307 100-101 0.8186541467689494 2201 1 0.11869314674528653 2201 2 0.19603980070910865 2201 3 0.22503386951944293 2201 4 0.28767648256056333 2201 5 0.2839114242040921 2201 6 0.29428556727742006 2201 7 0.2848203524217894 2201 8 0.2891178009671478 2201 9 0.30226856874544694 2201 10-11 0.29597334172245127 2201 12-13 0.29795385402585595 2201 14-15 0.5050400848177148 2201 16-17 0.5343520825857979 2201 18-19 0.5203213010175176 2201 20-21 0.5481653201569898 2201 22-23 0.5848175371648381 2201 24-25 0.5805322257972918 2201 26-27 0.5710163245864237 2201 28-29 0.5849382653218953 2201 30-31 0.5593281183234851 2201 32-33 0.59261323657379 2201 34-35 0.6148261040370215 2201 36-37 0.6236500287997515 2201 38-39 0.6143703383717707 2201 40-41 0.6502698355649841 2201 42-43 0.6178705160957563 2201 44-45 0.5507063566467423 2201 46-47 0.5779294308724943 2201 48-49 0.636449233243539 2201 50-51 0.5039745333990737 2201 52-53 0.6101953611744548 2201 54-55 0.6343350392055527 2201 56-57 0.6495795975218925 2201 58-59 0.6186242638701245 2201 60-61 0.6425770722956017 2201 62-63 0.6505808832114468 2201 64-65 0.6120996961490377 2201 66-67 0.6502204286075823 2201 68-69 0.6734038589472178 2201 70-71 0.6768796970809987 2201 72-73 0.6519667157425388 2201 74-75 0.6407367995146842 2201 76-77 0.7608594577468182 2201 78-79 0.7187063920522476 2201 80-81 0.7000561208226088 2201 82-83 0.6846749368535967 2201 84-85 0.7184994231830331 2201 86-87 0.7351060567004382 2201 88-89 0.7516114563980523 2201 90-91 0.77088209358039 2201 92-93 0.7356598072466127 2201 94-95 0.7528180609194095 2201 96-97 0.7421531491778168 2201 98-99 0.8097635604515148 2201 100-101 0.7711582427938382 1101 1 -0.014584456994192863 1101 2 0.17956163742589126 1101 3 0.22339438886744034 1101 4 0.28145628114162236 1101 5 0.316190914435289 1101 6 0.35717225764129523 1101 7 0.336525579668276 1101 8 0.35945276895061795 1101 9 0.352178912512386 1101 10-11 0.33820922663687014 1101 12-13 0.360879874561725 1101 14-15 0.5778444992497 1101 16-17 0.6221907172143659 1101 18-19 0.5998055438153926 1101 20-21 0.6068744895047828 1101 22-23 0.6365068087480665 1101 24-25 0.6443197164955521 1101 26-27 0.6619724881253575 1101 28-29 0.6397742640711499 1101 30-31 0.667095423138413 1101 32-33 0.6479350387260538 1101 34-35 0.6669944244123727 1101 36-37 0.6239746430499622 1101 38-39 0.710689892164794 1101 40-41 0.7092778590229827 1101 42-43 0.7022679150391724 1101 44-45 0.6976042403176876 1101 46-47 0.7337619339983448 1101 48-49 0.7510973769085396 1101 50-51 0.6332283256195907 1101 52-53 0.6779298975195758 1101 54-55 0.7552041888553305 1101 56-57 0.7226330297590664 1101 58-59 0.7270622070566759 1101 60-61 0.7161998892289816 1101 62-63 0.7119112438673483 1101 64-65 0.7114301738786182 1101 66-67 0.7522830482101526 1101 68-69 0.7630864275053284 1101 70-71 0.7704153735464345 1101 72-73 0.7823818472983675 1101 74-75 0.796101887309149 1101 76-77 0.8053718336393771 1101 78-79 0.8566514085279309 1101 80-81 0.8221099936718517 1101 82-83 0.8183537990949645 1101 84-85 0.8430834030029501 1101 86-87 0.8519528870202251 1101 88-89 0.7811506561882808 1101 90-91 0.8711175114162657 1101 92-93 0.872041879734681 1101 94-95 0.861801331348012 1101 96-97 0.8412380238293125 1101 98-99 0.8125191282880238 1101 100-101 0.8092711768380525 2304 1 -0.029864341753388146 2304 2 0.14262137777211592 2304 3 0.19362596527371068 2304 4 0.22602522030589256 2304 5 0.2462083066166585 2304 6 0.3050605983578478 2304 7 0.2705033990906003 2304 8 0.2949394114832984 2304 9 0.24851122138884563 2304 10-11 0.3075226464843013 2304 12-13 0.3406296762839389 2304 14-15 0.4913140768494557 2304 16-17 0.5287362223643157 2304 18-19 0.5353180557360275 2304 20-21 0.5328998853375495 2304 22-23 0.5412821584070571 2304 24-25 0.5879825674561374 2304 26-27 0.5160709099003782 2304 28-29 0.574427535117529 2304 30-31 0.5797168109096944 2304 32-33 0.5705558437973863 2304 34-35 0.5574957574499919 2304 36-37 0.5657978130878334 2304 38-39 0.6562345471599755 2304 40-41 0.6468195408362547 2304 42-43 0.6466658161598957 2304 44-45 0.6385414871748836 2304 46-47 0.6368832500841606 2304 48-49 0.6531948245192396 2304 50-51 0.5923152963000433 2304 52-53 0.636831815442271 2304 54-55 0.694473740463998 2304 56-57 0.6538342774133099 2304 58-59 0.6828892725254718 2304 60-61 0.6791792156813514 2304 62-63 0.7265543486948474 2304 64-65 0.6669279795004712 2304 66-67 0.6713592918973461 2304 68-69 0.7015535078195896 2304 70-71 0.7158202948265782 2304 72-73 0.7289777127003205 2304 74-75 0.7598364103636612 2304 76-77 0.7849164408070806 2304 78-79 0.7582122416966968 2304 80-81 0.7301729295773001 2304 82-83 0.7340830716810345 2304 84-85 0.7469640917691578 2304 86-87 0.7169650874862512 2304 88-89 0.7444067651171977 2304 90-91 0.7396585909080571 2304 92-93 0.735253767757591 2304 94-95 0.7433647885246586 2304 96-97 0.7281900294580126 2304 98-99 0.7465733713358915 2304 100-101 0.8536797787528272 2202 1 0.18648811040957014 2202 2 0.20511753745665118 2202 3 0.21473065389380253 2202 4 0.27114111681376585 2202 5 0.2792514503734438 2202 6 0.3010440496769107 2202 7 0.24577335544387324 2202 8 0.2626683169707036 2202 9 0.28864630975174776 2202 10-11 0.28447911707558404 2202 12-13 0.30628319103140456 2202 14-15 0.483373503543838 2202 16-17 0.4985659748600355 2202 18-19 0.5260829396321114 2202 20-21 0.512543815176123 2202 22-23 0.5267826665601945 2202 24-25 0.5565141312844517 2202 26-27 0.5219737340392072 2202 28-29 0.5707127185646996 2202 30-31 0.5648943760783922 2202 32-33 0.5713076220092859 2202 34-35 0.5738648347761242 2202 36-37 0.5260420247439797 2202 38-39 0.6039719369899075 2202 40-41 0.5796267887592421 2202 42-43 0.564050162709961 2202 44-45 0.5689471930086683 2202 46-47 0.57317667805669 2202 48-49 0.6484067613215814 2202 50-51 0.5677686317679687 2202 52-53 0.5905161201215563 2202 54-55 0.6423077539274686 2202 56-57 0.6264113624291383 2202 58-59 0.6089340813911903 2202 60-61 0.6413318048641656 2202 62-63 0.674319090645092 2202 64-65 0.7002319406319373 2202 66-67 0.6288241522006075 2202 68-69 0.6651631645439977 2202 70-71 0.6728927461858163 2202 72-73 0.7080251459436511 2202 74-75 0.6880900325751327 2202 76-77 0.6851267183338834 2202 78-79 0.7384322836711803 2202 80-81 0.726653547813001 2202 82-83 0.651059226724783 2202 84-85 0.6844993027629016 2202 86-87 0.7129642727560253 2202 88-89 0.7175647262045928 2202 90-91 0.670643325573046 2202 92-93 0.7329524141517041 2202 94-95 0.7555005418940937 2202 96-97 0.7322103536389761 2202 98-99 0.8057522114245117 2202 100-101 0.767045956235684 1102 1 0.14121096172132752 1102 2 0.19375522183544547 1102 3 0.18102173687194778 1102 4 0.24097052737678126 1102 5 0.25379349790568995 1102 6 0.25003026555626207 1102 7 0.2630810417578715 1102 8 0.24226629404920885 1102 9 0.26583721538850824 1102 10-11 0.2454537268551178 1102 12-13 0.27193533217576515 1102 14-15 0.3776046313064185 1102 16-17 0.42193323133297156 1102 18-19 0.42836082301634093 1102 20-21 0.4009353282333379 1102 22-23 0.38777662833956583 1102 24-25 0.422386773308439 1102 26-27 0.43335875213460184 1102 28-29 0.43141525991012486 1102 30-31 0.449455985280764 1102 32-33 0.439064647309408 1102 34-35 0.45431121146123843 1102 36-37 0.44642354814587293 1102 38-39 0.46225037118362167 1102 40-41 0.49134119460892833 1102 42-43 0.5126601762790344 1102 44-45 0.5017754240347045 1102 46-47 0.5201422533140132 1102 48-49 0.5350711013093061 1102 50-51 0.43865604846299533 1102 52-53 0.47010000820750264 1102 54-55 0.5473447210639222 1102 56-57 0.520044334893484 1102 58-59 0.5171063345901459 1102 60-61 0.5756287124259316 1102 62-63 0.5695092757527789 1102 64-65 0.5900746482572217 1102 66-67 0.5670464344275672 1102 68-69 0.5447275377106209 1102 70-71 0.5443280043961849 1102 72-73 0.6033753108889996 1102 74-75 0.6067637419908323 1102 76-77 0.6121125721069944 1102 78-79 0.6340007394580951 1102 80-81 0.5930583556657822 1102 82-83 0.6243228734972348 1102 84-85 0.6316715732454838 1102 86-87 0.6162702559240429 1102 88-89 0.5934267331288154 1102 90-91 0.6318892316900673 1102 92-93 0.5983796201734393 1102 94-95 0.6674100827700187 1102 96-97 0.6942726279777958 1102 98-99 0.6196097237546851 1102 100-101 0.5980884127666428 2305 1 0.15418167158573937 2305 2 0.12298689185340095 2305 3 0.17335213323082144 2305 4 0.17737754016302176 2305 5 0.2156143707118403 2305 6 0.17588291692258196 2305 7 0.21501549869152115 2305 8 0.21187532804911768 2305 9 0.22329200301641094 2305 10-11 0.22486297639864716 2305 12-13 0.19558700751810676 2305 14-15 0.31738608308430827 2305 16-17 0.3452883843920489 2305 18-19 0.39573934860550963 2305 20-21 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-1.0272225148956977 1213 84-85 -1.1101406365749469 1213 86-87 -1.0685377205489104 1213 88-89 -1.08803673623229 1213 90-91 -1.0734302795863542 1213 92-93 -1.0538539947611056 1213 94-95 -1.1859558023652923 1213 96-97 -1.277940162449994 1213 98-99 -1.0987597350167917 1213 100-101 -1.1304074276801046 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13869.0 3 1426.0 4 872.0 5 745.0 6 715.0 7 1295.0 8 1974.0 9 3033.0 10 5794.0 11 10245.0 12 13865.0 13 16903.0 14 19007.0 15 21228.0 16 23974.0 17 26740.0 18 29968.0 19 33068.0 20 36132.0 21 40175.0 22 44640.0 23 49659.0 24 56610.0 25 64295.0 26 74317.0 27 87348.0 28 104551.0 29 126441.0 30 155708.0 31 196697.0 32 258366.0 33 347121.0 34 486401.0 35 775538.0 36 1745134.0 37 5043349.0 38 5135006.0 39 4590.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 20.293778323644666 12.57403526659614 11.556878508194036 55.575307901565154 2 22.534584725552072 19.721014163799087 38.91895649027517 18.82544462037367 3 23.327528140749212 24.88214501167524 24.27103324645168 27.519293601123866 4 26.186039665017734 29.35846150438437 18.159460004359524 26.296038826238377 5 28.002570905791945 30.816524681906287 21.437248625242585 19.74365578705918 6 20.36136408529612 33.80576382889901 22.59305747838985 23.239814607415024 7 19.00703770555121 14.628237378130713 41.604577184807404 24.760147731510674 8 20.909552509377807 20.18447982093221 27.55861300652283 31.347354663167152 9 21.53384588281829 19.51090414128018 31.354732772919302 27.60051720298223 10-11 24.926302324618344 28.350006278153227 21.21422208762653 25.50946930960189 12-13 22.877132981357875 22.549974159076452 27.80553838348529 26.767354476080385 14-15 23.583324133326062 24.178254242090098 26.315633184404057 25.92278844017978 16-17 24.980171853799725 24.447002655071 24.885102506585632 25.687722984543644 18-19 24.42571429188967 24.836723187982436 24.915633677231792 25.821928842896103 20-21 24.470616152508555 24.962954464951174 25.268422754808068 25.298006627732196 22-23 24.392413030935536 24.94775835060754 25.253417934957728 25.406410683499193 24-25 24.166610881313144 25.109444877702376 25.302872900812112 25.421071340172368 26-27 24.26216771082629 25.170004509290532 25.442949427812895 25.124878352070283 28-29 24.498456710587334 24.800058681773542 25.15917863339699 25.542305974242137 30-31 24.284659229776686 24.84460480011562 25.2512716690975 25.619464301010197 32-33 24.49265761304292 25.0740940519914 25.280171822007265 25.15307651295842 34-35 24.695986036862568 25.004584912784257 24.92721326274629 25.37221578760689 36-37 24.47015413277798 25.04864207020624 24.943605721944014 25.53759807507176 38-39 24.584777688935276 25.159979672623372 25.218525283670907 25.036717354770445 40-41 24.74149216836453 24.9568238936393 25.04600994370908 25.255673994287093 42-43 24.297324262935994 24.845943655983245 25.368840851359188 25.48789122972157 44-45 24.640002223826915 25.00588293326893 25.246048259903652 25.1080665830005 46-47 24.799259685165957 24.869217188444132 24.98694616671666 25.344576959673255 48-49 24.5264804736917 24.822971951283414 25.084766626660045 25.565780948364843 50-51 24.635064786770812 25.044722481134702 25.195310258751608 25.124902473342882 52-53 24.89717695138728 24.7696683259798 24.96021484879249 25.372939873840426 54-55 24.440740573899724 24.825941263602015 25.168272431406685 25.565045731091573 56-57 24.585396365273755 25.00908859427049 25.303627202702067 25.10188783775369 58-59 24.761554288797967 24.917061096538593 24.908995687333274 25.412388927330166 60-61 24.48932984631828 24.923668488693096 25.095191009227673 25.49181065576095 62-63 24.72051648812276 25.020548034000406 25.16405019816728 25.09488527970955 64-65 24.7396223032687 24.967533515675157 24.926473524234922 25.366370656821225 66-67 24.587710409395648 24.91123812138386 25.078167713547078 25.422883755673414 68-69 24.746660330203042 25.118558473146358 25.117035540558106 25.017745656092494 70-71 24.88322806282132 24.914457457671254 24.915285320901067 25.287029158606362 72-73 24.66904283165236 24.941845272230694 24.9984978974388 25.390613998678152 74-75 24.763766668903052 25.117509199145232 25.0766455454077 25.042078586544015 76-77 24.886141305459915 24.99119531358109 24.874539249137673 25.24812413182132 78-79 24.680330292211664 24.96429449596362 25.01735711943934 25.33801809238538 80-81 24.8291627658632 25.16582571595872 24.94684531747951 25.05816620069857 82-83 24.98524298412081 25.025890653612866 24.75597017840919 25.23289618385714 84-85 24.65960761238256 25.03590200657535 25.01183432818391 25.29265605285818 86-87 24.85516311950056 25.25216323500964 24.98352316522274 24.909150480267066 88-89 24.94191933818869 25.034342986432982 24.743728535425664 25.280009139952664 90-91 24.851024514493623 24.92488206204393 24.864942018050655 25.35915140541179 92-93 24.896258842291832 25.19035973020617 24.91203003814409 25.00135138935791 94-95 25.09405095170018 25.005064224473163 24.769019666212998 25.13186515761366 96-97 24.796310465452688 25.064892138118903 24.887000208189445 25.251797188238967 98-99 24.9754923221356 25.157749318597617 24.92570395842261 24.94105440084417 100-101 25.21787737987891 25.00565586057364 24.614341786322402 25.162124973225048 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12207.0 1 6487.0 2 708.0 3 611.0 4 558.0 5 511.0 6 427.0 7 378.5 8 359.5 9 338.0 10 340.5 11 380.0 12 450.5 13 478.0 14 521.0 15 607.5 16 671.0 17 762.0 18 911.5 19 1094.0 20 1418.0 21 1897.5 22 2600.0 23 3740.0 24 5474.0 25 8137.0 26 12057.5 27 17414.5 28 24274.5 29 33286.0 30 44889.5 31 59462.0 32 77198.0 33 99954.5 34 129439.0 35 165841.0 36 209671.5 37 264469.5 38 328370.5 39 395416.0 40 462964.0 41 528381.0 42 586428.5 43 626194.0 44 645776.0 45 645913.5 46 630196.5 47 602698.5 48 569010.0 49 533910.0 50 496386.5 51 462721.0 52 438153.5 53 421869.0 54 412992.5 55 404663.0 56 394879.0 57 390856.5 58 381685.5 59 364463.0 60 346458.5 61 327461.5 62 314994.5 63 298776.0 64 265410.5 65 237361.0 66 223168.5 67 204817.0 68 181409.5 69 155806.5 70 129723.5 71 107496.5 72 89845.5 73 73818.0 74 55819.5 75 40849.5 76 30126.5 77 21302.5 78 14665.0 79 9932.5 80 6645.5 81 4352.0 82 2849.5 83 1726.0 84 1029.5 85 719.0 86 515.0 87 408.0 88 333.0 89 255.5 90 205.5 91 176.0 92 137.5 93 109.0 94 80.5 95 60.0 96 44.0 97 26.5 98 19.0 99 11.5 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1555642736547124 2 0.14839807584600154 3 0.15744382321899894 4 0.15307370444408536 5 0.15916397635380536 6 0.10671590953694739 7 0.11165719885083145 8 0.10784496757909832 9 0.09529914027543304 10-11 0.08345731386863835 12-13 0.09164962619212756 14-15 0.13667579676131694 16-17 0.14601045016274708 18-19 0.13377013268225207 20-21 0.16577560741828326 22-23 0.1467742247206727 24-25 0.1427295403226144 26-27 0.13326537732223162 28-29 0.1312496766410975 30-31 0.0947445735312001 32-33 0.10704798543169766 34-35 0.12183864578387477 36-37 0.12716846389461664 38-39 0.12586340562824808 40-41 0.16058526118333652 42-43 0.14045149968462753 44-45 0.13055563802106943 46-47 0.16481590808245497 48-49 0.18620491646332 50-51 0.16185711186023005 52-53 0.11051485777289051 54-55 0.11274972854455985 56-57 0.11519380712992185 58-59 0.1146624856983214 60-61 0.11926505759956016 62-63 0.10104405325461274 64-65 0.11840830179110447 66-67 0.13429149183700997 68-69 0.1329665090169564 70-71 0.12009856809538336 72-73 0.1297985049810388 74-75 0.16672534447726903 76-77 0.1945333799036568 78-79 0.1693786308763237 80-81 0.14236093607944159 82-83 0.12643125540827105 84-85 0.13306281102643397 86-87 0.12632167036300346 88-89 0.1296557123462962 90-91 0.14461905216374343 92-93 0.11105946224028096 94-95 0.11607712900995755 96-97 0.1169704131668358 98-99 0.12050702144592619 100-101 0.11350022006669545 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1.5056799E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.79373285524795 #Duplication Level Percentage of deduplicated Percentage of total 1 78.50150163561301 46.93897817535655 2 11.826182096152396 14.142631459097052 3 4.019345215052941 7.209949621256842 4 1.855979002492255 4.4390365063988595 5 1.1024450630340925 3.295965279332376 6 0.6033861377811834 2.164722571862875 7 0.3954277688788762 1.655087166312318 8 0.3330809299311022 1.5932921714782309 9 0.19897656981310133 1.0707796673872358 >10 1.0768883172872854 11.181158174532145 >50 0.05975658995152548 2.4430173519970206 >100 0.025424973030224788 2.848388123653709 >500 0.0010544388402397107 0.43317902442021144 >1k 5.283675993316943E-4 0.49235927638315313 >5k 2.289454250282525E-5 0.09145543053142642 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA Adapter Nextera Transposase Sequence 1 0.010420541577263534 2.6566071580021755E-5 0.0 2 0.011151108545714132 2.6566071580021755E-5 6.641517895005439E-6 3 0.014046810347936504 2.6566071580021755E-5 6.641517895005439E-6 4 0.023378142990419147 2.6566071580021755E-5 1.3283035790010878E-5 5 0.025038522464170505 2.6566071580021755E-5 1.3283035790010878E-5 6 0.03027203856543479 3.32075894750272E-5 1.9924553685016317E-5 7 0.031374530536005696 5.977366105504895E-5 1.9924553685016317E-5 8 0.03396472251505782 5.977366105504895E-5 1.9924553685016317E-5 9 0.03763748191099582 7.305669684505983E-5 2.6566071580021755E-5 10-11 0.04553756744710479 7.969821474006527E-5 2.6566071580021755E-5 12-13 0.05401878579902674 9.962276842508158E-5 2.6566071580021755E-5 14-15 0.0633667222362469 1.0958504526758974E-4 3.9849107370032635E-5 16-17 0.0729072626924222 1.2950959895260608E-4 3.9849107370032635E-5 18-19 0.0824278785949125 1.3947187579511423E-4 3.9849107370032635E-5 20-21 0.09063347395419172 1.5607567053262783E-4 3.9849107370032635E-5 22-23 0.09834759698924053 1.7600022421764412E-4 3.9849107370032635E-5 24-25 0.10530790774320625 1.89283260007655E-4 4.649062526503807E-5 26-27 0.11173025554767649 1.9260401895515773E-4 4.981138421254079E-5 28-29 0.11715969642684344 2.0256629579766587E-4 6.64151789500544E-5 30-31 0.1221142687765175 2.2581160843018492E-4 1.0626428632008702E-4 32-33 0.12628846277352843 2.4573616211520123E-4 1.195473221100979E-4 34-35 0.1299379768568339 2.656607158002176E-4 1.4611339369011966E-4 36-37 0.13352439652013687 2.656607158002176E-4 1.527549115851251E-4 38-39 0.13733662779186995 2.72302233695223E-4 1.5939642948013054E-4 40-41 0.14085331151727534 2.8558526948523386E-4 2.0256629579766587E-4 42-43 0.14364606979212513 3.0883058211775294E-4 2.2581160843018492E-4 44-45 0.1464321865490799 3.420381715927801E-4 2.4905692106270397E-4 46-47 0.14940094504814735 3.586419663302937E-4 2.6233995685271484E-4 48-49 0.15257559060195997 3.984910737003264E-4 2.7894375159022845E-4 50-51 0.15565061338734748 4.283779042278508E-4 2.9222678738023933E-4 52-53 0.15857620202009737 4.4498169896536443E-4 3.1547210001275835E-4 54-55 0.16127929980336458 4.6822701159788345E-4 3.254343768552665E-4 56-57 0.1639691145508418 4.8483080633539706E-4 3.3207589475027196E-4 58-59 0.16671538220042653 5.014346010729107E-4 3.55321207382791E-4 60-61 0.1697140275300215 5.280006726529324E-4 3.586419663302937E-4 62-63 0.172789050315409 5.545667442329542E-4 3.719250021203046E-4 64-65 0.17593048827974656 5.645290210754624E-4 3.7856652001531E-4 66-67 0.17908853003882166 5.645290210754624E-4 3.918495558053209E-4 68-69 0.18225653407473927 5.645290210754624E-4 3.918495558053209E-4 70-71 0.18575329324645962 6.043781284454949E-4 4.084533505428345E-4 72-73 0.1896186566613528 6.176611642355059E-4 4.250571452803481E-4 74-75 0.1938957941857363 6.342649589730194E-4 4.283779042278508E-4 76-77 0.198431950908025 6.674725484480466E-4 4.3169866317535355E-4 78-79 0.2037850143313994 6.707933073955494E-4 4.3501942212285623E-4 80-81 0.2094269837832065 6.741140663430521E-4 4.4498169896536443E-4 82-83 0.21645703047506976 6.774348252905547E-4 4.8815156528289974E-4 84-85 0.22525704168595198 6.807555842380575E-4 4.914723242304025E-4 86-87 0.23613584799797088 7.139631737130847E-4 5.047553600204134E-4 88-89 0.24913329851849653 7.405292452931065E-4 5.113968779154188E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 5385 0.0 12.836361 14-15 CTCGGAT 7945 0.0 12.1973505 1 CTCGGGT 8670 0.0 11.78009 1 CTCGGTT 6515 0.0 11.520515 1 CGCGTAT 1130 0.0 11.350489 1 CTCGACT 7690 0.0 11.304569 1 CTCGTTT 6795 0.0 11.255523 1 CTCGATT 8415 0.0 11.177388 1 CTCGTAT 4075 0.0 10.957954 1 CGCAAAT 3500 0.0 10.858034 1 CTGGGAT 10575 0.0 10.241976 1 TTAGGTG 7010 0.0 9.962681 18-19 CTGGAAT 14185 0.0 9.912692 1 CGGGAAT 4435 0.0 9.854247 1 TTACTCG 4295 0.0 9.843616 3 GAGTGTC 7320 0.0 9.668882 24-25 AATTAGG 7465 0.0 9.610047 16-17 CTCAGAT 12190 0.0 9.586513 1 CTCAAAT 12245 0.0 9.543454 1 CGACAAT 5580 0.0 9.534832 1 >>END_MODULE