FastQCFastQC Report
Wed 17 Jun 2015
C5B16ACXX l06n02 kx12-2.3420000000cd0a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC5B16ACXX l06n02 kx12-2.3420000000cd0a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17067317
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT121200.026.1699128
GAGCGTC123750.025.4830079
TTAAAAA233250.023.37209956-57
AAGAGCG158950.020.9689037
ATCGGAA175600.020.3970032
TCGGGGG115950.020.34286138-39
CCGTATC96350.019.97348-49
TCGTGTA93750.018.95252214-15
CGCCGTA100350.018.82198746-47
TTTAAAA146450.018.64472456-57
TCGGAAG198000.017.8252983
GTAGGGA105200.017.70254718-19
GTGTAGG112050.017.27771616-17
GATCGGA211800.017.04551
GCCGTAT85500.017.00595546-47
ATCGGGA93050.016.8978392
GGGGCGC81200.016.6479442-43
CGGAAGA212000.016.6255554
CATTAAA146050.016.27329654-55
CGGGAGA100450.015.1324114
Produced by FastQC (version 0.11.2)